General Information of Target

Target ID LDTP12331
Target Name Endoplasmic reticulum membrane protein complex subunit 7 (EMC7)
Gene Name EMC7
Gene ID 56851
Synonyms
C11orf3; C15orf24; Endoplasmic reticulum membrane protein complex subunit 7; ER membrane protein complex subunit 7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRKTRLWGLLWMLFVSELRAATKLTEEKYELKEGQTLDVKCDYTLEKFASSQKAWQIIRD
GEMPKTLACTERPSKNSHPVQVGRIILEDYHDHGLLRVRMVNLQVEDSGLYQCVIYQPPK
EPHMLFDRIRLVVTKGFSGTPGSNENSTQNVYKIPPTTTKALCPLYTSPRTVTQAPPKST
ADVSTPDSEINLTNVTDIIRVPVFNIVILLAGGFLSKSLVFSVLFAVTLRSFVP
Target Bioclass
Transporter and channel
Family
EMC7 family
Subcellular location
Endoplasmic reticulum membrane
Function
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Uniprot ID
Q9NPA0
Ensemble ID
ENST00000256545.9
HGNC ID
HGNC:24301

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Jackson_1
 Probe Info 
20.00  LDD0122  [1]
STPyne
 Probe Info 
K141(3.45); K221(5.28); K225(10.00); K232(1.22)  LDD0277  [2]
Jackson_14
 Probe Info 
2.63  LDD0123  [1]
HPAP
 Probe Info 
3.86  LDD0063  [3]
Acrolein
 Probe Info 
H87(0.00); H74(0.00)  LDD0227  [4]
m-APA
 Probe Info 
N.A.  LDD2231  [5]
PAL-AfBPP Probe
Click To Hide/Show 42 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info 
8.69  LDD1728  [6]
C040
 Probe Info 
14.72  LDD1740  [6]
C041
 Probe Info 
5.70  LDD1741  [6]
C055
 Probe Info 
12.04  LDD1752  [6]
C056
 Probe Info 
22.32  LDD1753  [6]
C091
 Probe Info 
14.62  LDD1782  [6]
C092
 Probe Info 
31.78  LDD1783  [6]
C094
 Probe Info 
64.89  LDD1785  [6]
C100
 Probe Info 
5.46  LDD1789  [6]
C106
 Probe Info 
23.92  LDD1793  [6]
C112
 Probe Info 
28.44  LDD1799  [6]
C134
 Probe Info 
26.54  LDD1816  [6]
C159
 Probe Info 
7.94  LDD1839  [6]
C161
 Probe Info 
11.16  LDD1841  [6]
C201
 Probe Info 
37.01  LDD1877  [6]
C210
 Probe Info 
39.12  LDD1884  [6]
C218
 Probe Info 
13.45  LDD1892  [6]
C220
 Probe Info 
14.52  LDD1894  [6]
C228
 Probe Info 
14.83  LDD1901  [6]
C231
 Probe Info 
16.56  LDD1904  [6]
C232
 Probe Info 
56.49  LDD1905  [6]
C235
 Probe Info 
52.35  LDD1908  [6]
C264
 Probe Info 
25.11  LDD1935  [6]
C265
 Probe Info 
16.00  LDD1936  [6]
C270
 Probe Info 
6.50  LDD1940  [6]
C285
 Probe Info 
25.81  LDD1955  [6]
C287
 Probe Info 
13.36  LDD1957  [6]
C288
 Probe Info 
6.73  LDD1958  [6]
C289
 Probe Info 
73.52  LDD1959  [6]
C296
 Probe Info 
13.18  LDD1966  [6]
C299
 Probe Info 
7.46  LDD1968  [6]
C314
 Probe Info 
11.39  LDD1981  [6]
C338
 Probe Info 
14.03  LDD2001  [6]
C349
 Probe Info 
12.21  LDD2010  [6]
C350
 Probe Info 
41.93  LDD2011  [6]
C362
 Probe Info 
81.01  LDD2023  [6]
C364
 Probe Info 
18.00  LDD2025  [6]
C388
 Probe Info 
75.06  LDD2047  [6]
C390
 Probe Info 
26.72  LDD2049  [6]
C407
 Probe Info 
14.03  LDD2064  [6]
FFF probe11
 Probe Info 
20.00  LDD0471  [7]
FFF probe13
 Probe Info 
20.00  LDD0476  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0109  NEM HeLa H87(0.00); H74(0.00)  LDD0227  [4]
 LDCM0014  Panhematin K562 3.86  LDD0063  [3]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.63  LDD0123  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Keratin-associated protein 1-1 (KRTAP1-1) KRTAP type 1 family Q07627
Keratin-associated protein 5-2 (KRTAP5-2) KRTAP type 5 family Q701N4
Keratin-associated protein 5-9 (KRTAP5-9) KRTAP type 5 family P26371
Notch homolog 2 N-terminal-like protein C (NOTCH2NLC) NOTCH family P0DPK4

References

1 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.