Details of the Target

General Information of Target

Target ID LDTP03284
Target Name ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB)
Gene Name ATP5PB
Gene ID 515
Synonyms
ATP5F1; ATP synthase F(0) complex subunit B1, mitochondrial; ATP synthase peripheral stalk-membrane subunit b; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATPase subunit b
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLSRVVLSAAATAAPSLKNAAFLGPGVLQATRTFHTGQPHLVPVPPLPEYGGKVRYGLIP
EEFFQFLYPKTGVTGPYVLGTGLILYALSKEIYVISAETFTALSVLGVMVYGIKKYGPFV
ADFADKLNEQKLAQLEEAKQASIQHIQNAIDTEKSQQALVQKRHYLFDVQRNNIAMALEV
TYRERLYRVYKEVKNRLDYHISVQNMMRRKEQEHMINWVEKHVVQSISTQQEKETIAKCI
ADLKLLAKKAQAQPVM
Target Bioclass
Transporter and channel
Family
Eukaryotic ATPase B chain family
Subcellular location
Mitochondrion
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Uniprot ID
P24539
Ensemble ID
ENST00000369722.8
HGNC ID
HGNC:840

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 29 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		9.03  LDD0402  [2]
HDSF-alk
 Probe Info  		3.29  LDD0197  [3]
CY4
 Probe Info  		100.00  LDD0244  [4]
N1
 Probe Info  		100.00  LDD0242  [4]
TH211
 Probe Info  		Y56(12.30); Y116(11.28)  LDD0260  [5]
YN-1
 Probe Info  		100.00  LDD0444  [6]
YN-4
 Probe Info  		100.00  LDD0445  [6]
ONAyne
 Probe Info  		K162(0.00); K53(0.00)  LDD0273  [7]
DBIA
 Probe Info  		C239(15.53)  LDD3319  [8]
BTD
 Probe Info  		C239(0.09)  LDD2116  [9]
Jackson_14
 Probe Info  		2.50  LDD0123  [10]
W1
 Probe Info  		D198(2.44)  LDD0238  [11]
5E-2FA
 Probe Info  		H222(0.00); H200(0.00); H145(0.00)  LDD2235  [12]
ATP probe
 Probe Info  		N.A.  LDD0199  [13]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [14]
ATP probe
 Probe Info  		N.A.  LDD0035  [15]
TFBX
 Probe Info  		N.A.  LDD0027  [16]
WYneN
 Probe Info  		N.A.  LDD0021  [17]
IPM
 Probe Info  		N.A.  LDD0147  [16]
NHS
 Probe Info  		K162(0.00); K131(0.00)  LDD0010  [17]
SF
 Probe Info  		N.A.  LDD0028  [18]
STPyne
 Probe Info  		K162(0.00); K131(0.00)  LDD0009  [17]
Ox-W18
 Probe Info  		N.A.  LDD2175  [19]
1c-yne
 Probe Info  		N.A.  LDD0228  [20]
Acrolein
 Probe Info  		H222(0.00); H164(0.00); H145(0.00)  LDD0217  [21]
AOyne
 Probe Info  		8.90  LDD0443  [22]
MPP-AC
 Probe Info  		N.A.  LDD0428  [23]
TER-AC
 Probe Info  		N.A.  LDD0426  [23]
TPP-AC
 Probe Info  		N.A.  LDD0427  [23]
PAL-AfBPP Probe
Click To Hide/Show 82 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		7.62  LDD1714  [24]
C022
 Probe Info  		16.91  LDD1728  [24]
C027
 Probe Info  		5.50  LDD1733  [24]
C040
 Probe Info  		18.38  LDD1740  [24]
C041
 Probe Info  		7.26  LDD1741  [24]
C053
 Probe Info  		6.15  LDD1751  [24]
C056
 Probe Info  		66.26  LDD1753  [24]
C063
 Probe Info  		13.09  LDD1760  [24]
C070
 Probe Info  		13.00  LDD1766  [24]
C091
 Probe Info  		15.89  LDD1782  [24]
C092
 Probe Info  		19.43  LDD1783  [24]
C094
 Probe Info  		39.12  LDD1785  [24]
C106
 Probe Info  		31.78  LDD1793  [24]
C107
 Probe Info  		5.98  LDD1794  [24]
C108
 Probe Info  		10.70  LDD1795  [24]
C112
 Probe Info  		23.26  LDD1799  [24]
C134
 Probe Info  		35.26  LDD1816  [24]
C143
 Probe Info  		49.18  LDD1825  [24]
C153
 Probe Info  		29.86  LDD1834  [24]
C159
 Probe Info  		9.92  LDD1839  [24]
C160
 Probe Info  		7.46  LDD1840  [24]
C161
 Probe Info  		17.75  LDD1841  [24]
C163
 Probe Info  		7.06  LDD1843  [24]
C165
 Probe Info  		13.64  LDD1845  [24]
C178
 Probe Info  		26.91  LDD1857  [24]
C187
 Probe Info  		32.45  LDD1865  [24]
C191
 Probe Info  		13.18  LDD1868  [24]
C193
 Probe Info  		5.78  LDD1869  [24]
C198
 Probe Info  		11.24  LDD1874  [24]
C201
 Probe Info  		99.73  LDD1877  [24]
C206
 Probe Info  		47.84  LDD1881  [24]
C207
 Probe Info  		18.90  LDD1882  [24]
C213
 Probe Info  		14.93  LDD1887  [24]
C218
 Probe Info  		17.03  LDD1892  [24]
C220
 Probe Info  		20.97  LDD1894  [24]
C228
 Probe Info  		22.94  LDD1901  [24]
C229
 Probe Info  		17.51  LDD1902  [24]
C231
 Probe Info  		19.56  LDD1904  [24]
C233
 Probe Info  		9.51  LDD1906  [24]
C235
 Probe Info  		33.36  LDD1908  [24]
C243
 Probe Info  		12.21  LDD1916  [24]
C246
 Probe Info  		13.93  LDD1919  [24]
C264
 Probe Info  		24.76  LDD1935  [24]
C265
 Probe Info  		13.27  LDD1936  [24]
C277
 Probe Info  		14.32  LDD1947  [24]
C278
 Probe Info  		99.73  LDD1948  [24]
C287
 Probe Info  		15.56  LDD1957  [24]
C289
 Probe Info  		39.67  LDD1959  [24]
C293
 Probe Info  		51.98  LDD1963  [24]
C296
 Probe Info  		11.96  LDD1966  [24]
C299
 Probe Info  		5.86  LDD1968  [24]
C314
 Probe Info  		14.12  LDD1981  [24]
C339
 Probe Info  		9.99  LDD2002  [24]
C343
 Probe Info  		19.16  LDD2005  [24]
C348
 Probe Info  		13.74  LDD2009  [24]
C349
 Probe Info  		12.73  LDD2010  [24]
C350
 Probe Info  		74.54  LDD2011  [24]
C355
 Probe Info  		24.25  LDD2016  [24]
C362
 Probe Info  		99.73  LDD2023  [24]
C363
 Probe Info  		16.00  LDD2024  [24]
C364
 Probe Info  		21.56  LDD2025  [24]
C366
 Probe Info  		8.06  LDD2027  [24]
C367
 Probe Info  		5.58  LDD2028  [24]
C376
 Probe Info  		10.41  LDD2036  [24]
C388
 Probe Info  		78.79  LDD2047  [24]
C390
 Probe Info  		99.73  LDD2049  [24]
C407
 Probe Info  		13.64  LDD2064  [24]
C431
 Probe Info  		20.39  LDD2086  [24]
FFF probe11
 Probe Info  		20.00  LDD0471  [25]
FFF probe13
 Probe Info  		20.00  LDD0475  [25]
FFF probe14
 Probe Info  		20.00  LDD0477  [25]
FFF probe2
 Probe Info  		20.00  LDD0463  [25]
FFF probe3
 Probe Info  		20.00  LDD0464  [25]
FFF probe4
 Probe Info  		14.28  LDD0466  [25]
JN0003
 Probe Info  		20.00  LDD0469  [25]
STS-1
 Probe Info  		N.A.  LDD0136  [26]
STS-2
 Probe Info  		N.A.  LDD0138  [26]
BD-F
 Probe Info  		Y77(0.00); G75(0.00); Y86(0.00); G117(0.00)  LDD0024  [27]
LD-F
 Probe Info  		I59(0.00); V73(0.00); L58(0.00); P76(0.00)  LDD0015  [27]
TM-F
 Probe Info  		T81(0.00); G80(0.00); L85(0.00)  LDD0020  [27]
DA-2
 Probe Info  		N.A.  LDD0073  [28]
OEA-DA
 Probe Info  		14.62  LDD0046  [29]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H222(0.00); H164(0.00)  LDD0222  [21]
 LDCM0107  IAA HeLa H222(0.00); H164(0.00); H145(0.00); H214(0.00)  LDD0221  [21]
 LDCM0022  KB02 42-MG-BA C239(1.84)  LDD2244  [8]
 LDCM0023  KB03 42-MG-BA C239(6.55)  LDD2661  [8]
 LDCM0024  KB05 MEWO C239(15.53)  LDD3319  [8]
 LDCM0109  NEM HeLa H222(0.00); H145(0.00); H200(0.00); H164(0.00)  LDD0223  [21]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C239(0.09)  LDD2116  [9]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C239(0.09)  LDD2118  [9]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C239(0.79)  LDD2120  [9]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C239(0.04)  LDD2122  [9]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C239(0.05)  LDD2124  [9]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C239(0.05)  LDD2126  [9]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C239(0.03)  LDD2149  [9]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.50  LDD0123  [10]
 LDCM0111  W14 Hep-G2 D198(2.44)  LDD0238  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein-arginine deiminase type-3 (PADI3) Protein arginine deiminase family Q9ULW8
Methyltransferase-like protein 27 (METTL27) . Q8N6F8
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP synthase subunit alpha, mitochondrial (ATP5F1A) ATPase alpha/beta chains family P25705
ATP synthase subunit d, mitochondrial (ATP5PD) ATPase d subunit family O75947
Huntingtin (HTT) Huntingtin family P42858
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
DAP3-binding cell death enhancer 1 (DELE1) DELE1 family Q14154
Kinetochore-associated protein DSN1 homolog (DSN1) . Q9H410
Protein FAM117B (FAM117B) . Q6P1L5

References

1 Labeling Preferences of Diazirines with Protein Biomolecules. J Am Chem Soc. 2021 May 5;143(17):6691-6700. doi: 10.1021/jacs.1c02509. Epub 2021 Apr 20.
Mass spectrometry data entry: PXD025140
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
9 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
10 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
11 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
12 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
15 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
16 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
19 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
20 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
21 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
22 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
23 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
24 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
25 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
26 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
27 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652
28 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
29 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570