Details of the Probe

General Information of Probe

Probe ID
LDPC0015
Probe Name
LD-F
Synonyms
FFF-34
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 386.5
Lipid-water partition coefficient (xlogp) 3.7
Rotatable Bond Count (rotbonds) 9
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 4
Chemical Identifiers
Formula
C24H26N4O
IUPAC Name
1-benzhydryl-N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]azetidine-3-carboxamide
Canonical SMILES
C#CCCC1(N=N1)CCNC(=O)C2CN(C2)C(C3=CC=CC=C3)C4=CC=CC=C4
InChI
InChI=1S/C24H26N4O/c1-2-3-14-24(26-27-24)15-16-25-23(29)21-17-28(18-21)22(19-10-6-4-7-11-19)20-12-8-5-9-13-20/h1,4-13,21-22H,3,14-18H2,(H,25,29)
InChIKey
UDMXAOCUXYXPSG-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

51 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
60 kDa heat shock protein, mitochondrial (HSPD1) LDTP02522 N106; T105; A109; E108  LDD0015 
Acetyl-CoA acetyltransferase, cytosolic (ACAT2) LDTP11321 G244; L234; K235  LDD0015 
Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) LDTP03291 M335; K338  LDD0015 
Actin, cytoplasmic 1 (ACTB) LDTP04876 E226; D222  LDD0015 
ADP-dependent glucokinase (ADPGK) LDTP11127 S38; W36  LDD0015 
ADP-ribosylation factor 3 (ARF3) LDTP04907 E57; E41; I42; P47  LDD0015 
Alkyldihydroxyacetonephosphate synthase, peroxisomal (AGPS) LDTP00186 S392; A394; V393  LDD0015 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 E601; A605  LDD0015 
Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G) LDTP05920 L457; E461; S459  LDD0015 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 Y486  LDD0015 
Cathepsin B (CTSB) LDTP02223 E324; S323; E322; I321  LDD0015 
Cathepsin D (CTSD) LDTP02198 Q240; E324; G323; P237  LDD0015 
CDGSH iron-sulfur domain-containing protein 1 (CISD1) LDTP12873 I14; E12  LDD0015 
Creatine kinase B-type (CKB) LDTP02616 E181; E160; A161; A182  LDD0015 
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) LDTP03610 E411; V409; P408  LDD0015 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 H46; Y44  LDD0015 
Cytochrome c oxidase subunit 2 (MT-CO2) LDTP01773 T63; L50; E60  LDD0015 
Delta(24)-sterol reductase (DHCR24) LDTP06375 D341; W337  LDD0015 
E3 ubiquitin-protein ligase TRIP12 (TRIP12) LDTP06173 Q534; D537; I536; C535  LDD0015 
Elongation factor 2 (EEF2) LDTP02672 L174; E742; E170; E748  LDD0015 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 N574; E575  LDD0015 
Epoxide hydrolase 1 (EPHX1) LDTP02175 P318; T305; H301; Y299  LDD0015 
Exonuclease 1 (EXO1) LDTP13751 D152; A151; P148; E150  LDD0015 
Fatty acid synthase (FASN) LDTP04243 A2453; D2457  LDD0015 
Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) LDTP17305 V37; I42  LDD0015 
Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase (BPNT2) LDTP12763 E44  LDD0015 
GPI-anchor transamidase (PIGK) LDTP09835 S175; E177; F172  LDD0015 
Heat shock protein HSP 90-alpha (HSP90AA1) LDTP02225 V530; P524; E527; D526  LDD0015 
Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) LDTP06645 M256; Y264; E260  LDD0015 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 C265  LDD0015 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 I225; L221; S229  LDD0015 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 M77; I256; E78; V80  LDD0015 
Presequence protease, mitochondrial (PITRM1) LDTP07003 S375; G382; F374  LDD0015 
Proteasome subunit alpha type-2 (PSMA2) LDTP03331 Y97; Q96; Q95; E103  LDD0015 
Proteasome subunit alpha type-4 (PSMA4) LDTP03333 E89; T80  LDD0015 
Proteasome subunit beta type-1 (PSMB1) LDTP03060 Y132; V134; P131; Y133  LDD0015 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 D108; E109  LDD0015 
Proteasome subunit beta type-6 (PSMB6) LDTP03418 G98; Y95  LDD0015 
Pyruvate kinase PKM (PKM) LDTP02733 T409; P408; E418; A413  LDD0015 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 A256; T264; E261; M258  LDD0015 
rRNA 2'-O-methyltransferase fibrillarin (FBL) LDTP03155 S271; C268; D270  LDD0015 
Serine hydroxymethyltransferase, mitochondrial (SHMT2) LDTP03689 Y105; Y106  LDD0015 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 I149; Y148  LDD0015 
Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3) LDTP02671 E352; A353; V354; E355  LDD0015 
Transmembrane protein with metallophosphoesterase domain (TMPPE) LDTP07831 D239; V254; S250; D246  LDD0015 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 E524; S525; W542; Q534  LDD0015 
Tubulin alpha-1C chain (TUBA1C) LDTP11074 L378; M377  LDD0015 
Tubulin alpha-3D chain (TUBA3D) LDTP02453 C295; E297; I355; V375  LDD0015 
Very long chain fatty acid elongase 2 (ELOVL2) LDTP12773 I11; D8  LDD0015 
Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12) LDTP06901 G46; V39; E42; V36  LDD0015 
X-ray repair cross-complementing protein 6 (XRCC6) LDTP02640 A494; D13; D496; N23  LDD0015 
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⏷ Show the Full List of 51 Enzyme
36 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
14-3-3 protein epsilon (YWHAE) LDTP04969 T208; I203; A204; E205  LDD0015 
14-3-3 protein zeta/delta (YWHAZ) LDTP05043 T205; S207; L203; A201  LDD0015 
Annexin A1 (ANXA1) LDTP02010 E17; I14; E15; E18  LDD0015 
ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) LDTP03284 I59; V73; L58; P76  LDD0015 
ATP synthase subunit a (MT-ATP6) LDTP01804 T146; I147; M140; P139  LDD0015 
ATP synthase subunit alpha, mitochondrial (ATP5F1A) LDTP03327 V443  LDD0015 
BRI3-binding protein (BRI3BP) LDTP15648 T96; L99; L98; V101  LDD0015 
CD63 antigen (CD63) LDTP02320 D109  LDD0015 
Complement component 1 Q subcomponent-binding protein, mitochondrial (C1QBP) LDTP05510 E192; P160; Q195; P189  LDD0015 
Cytochrome c oxidase subunit 5A, mitochondrial (COX5A) LDTP03064 T79; Y80  LDD0015 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 G64; Y71; E72; A74  LDD0015 
Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial (SLC25A13) LDTP13393 T265; M267; I271; L272  LDD0015 
Exportin-2 (CSE1L) LDTP04662 E34; S35  LDD0015 
Heat shock protein HSP 90-beta (HSP90AB1) LDTP02262 G687; P516; E519; V695  LDD0015 
Importin subunit alpha-1 (KPNA2) LDTP04501 F138; E139; S134; A143  LDD0015 
Membrane-associated progesterone receptor component 1 (PGRMC1) LDTP00235 P51; D49; D56  LDD0015 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 A87; M85; V88  LDD0015 
Mitochondrial carrier homolog 1 (MTCH1) LDTP12909 G70; A83; E82; P79  LDD0015 
Mitochondrial carrier homolog 2 (MTCH2) LDTP14235 V91; Y95; Q96; Q93  LDD0015 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 A20; G18; D16  LDD0015 
Mitochondrial import inner membrane translocase subunit Tim23B (TIMM23B) LDTP19379 S139; D154; S133; Y138  LDD0015 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 P182; Y184  LDD0015 
Multiple coagulation factor deficiency protein 2 (MCFD2) LDTP09278 D115; E104; A109  LDD0015 
Peroxisomal membrane protein PEX16 (PEX16) LDTP14181 L46; A41; D42; E45  LDD0015 
Protein spinster homolog 1 (SPNS1) LDTP11844 L429; G433; D431  LDD0015 
Putative lipid scramblase CLPTM1 (CLPTM1) LDTP01745 D339; S338; D554; Y335  LDD0015 
Sideroflexin-4 (SFXN4) LDTP07531 D51; E48; L50; T47  LDD0015 
Solute carrier family 25 member 3 (SLC25A3) LDTP05238 S169; E173; M207; A168  LDD0015 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 V230; Y227  LDD0015 
T-complex protein 1 subunit delta (CCT4) LDTP04391 S463; T464; N468; P462  LDD0015 
Transmembrane protein 126A (TMEM126A) LDTP15948 Q177; E176  LDD0015 
Vacuole membrane protein 1 (VMP1) LDTP10343 Q225; E229; E222  LDD0015 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 H181; N183; I123; L125  LDD0015 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 K45; C47; V217; S216  LDD0015 
Voltage-dependent anion-selective channel protein 3 (VDAC3) LDTP13824 S35; C36; S37; G38  LDD0015 
Zinc transporter SLC39A7 (SLC39A7) LDTP09769 D326; F331; A328; A324  LDD0015 
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⏷ Show the Full List of 36 Transporter and channel
1 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Non-POU domain-containing octamer-binding protein (NONO) LDTP06341 V159; S160; F168; Y158  LDD0015 
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1 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Basigin (BSG) LDTP03772 E293; E288  LDD0015 
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40 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
26S proteasome non-ATPase regulatory subunit 11 (PSMD11) LDTP00223 F236; A235  LDD0015 
Elongation factor 1-gamma (EEF1G) LDTP03367 E261; D264  LDD0015 
Endoplasmin (HSP90B1) LDTP02734 D573; V572; Y575; P571  LDD0015 
Eukaryotic translation initiation factor 3 subunit L (EIF3L) LDTP13813 V246; Y247; D252  LDD0015 
F-actin-capping protein subunit beta (CAPZB) LDTP04127 P33; L39; S42  LDD0015 
FUN14 domain-containing protein 1 (FUNDC1) LDTP08509 N125; E130; I124  LDD0015 
Ganglioside GM2 activator (GM2A) LDTP02927 D156; L157  LDD0015 
Heat shock 70 kDa protein 1A (HSPA1A) LDTP02434 G372; A370; P365  LDD0015 
Heat shock protein 75 kDa, mitochondrial (TRAP1) LDTP05697 Q671; A676  LDD0015 
Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) LDTP02364 G241; N249; G246; N245  LDD0015 
Heterogeneous nuclear ribonucleoprotein F (HNRNPF) LDTP04514 G241; D251; S250  LDD0015 
Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) LDTP03615 C267; Y266; D245; Y240  LDD0015 
Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2) LDTP04714 G238; D244  LDD0015 
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) LDTP04952 A447; D382; P106; L114  LDD0015 
Heterogeneous nuclear ribonucleoprotein L (HNRNPL) LDTP02750 P370; E120; S129; P369  LDD0015 
Heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1) LDTP03182 G337; G334; N335; N245  LDD0015 
Histone H2A type 2-C (H2AC20) LDTP06627 E57; Y58; E62; L59  LDD0015 
Histone H2A type 3 (H2AC25) LDTP07973 L56; E62; A54; T60  LDD0015 
Histone H2A.V (H2AZ2) LDTP07908 E60; E65; T63; L59  LDD0015 
Histone H3.2 (H3C15; H3C14; H3C13) LDTP07899 D107; F105; A99  LDD0015 
Inversin (INVS) LDTP13829 V649; N648  LDD0015 
Large ribosomal subunit protein P1 (RPLP1) LDTP02107 N53; G64; N62; A69  LDD0015 
Large ribosomal subunit protein uL2 (RPL8) LDTP05027 G131; N132  LDD0015 
MICOS complex subunit MIC13 (MICOS13) LDTP07274 D27; L24  LDD0015 
Microtubule-associated protein RP/EB family member 1 (MAPRE1) LDTP06444 L226; E225; I227; N231  LDD0015 
Mitochondrial import receptor subunit TOM20 homolog (TOMM20) LDTP06373 Q102; G101  LDD0015 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 T66; A65  LDD0015 
Nascent polypeptide-associated complex subunit alpha (NACA) LDTP05977 S132; D130; L131  LDD0015 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 V483; G481; L479; D478  LDD0015 
Proliferating cell nuclear antigen (PCNA) LDTP02596 G127; V123; E124  LDD0015 
Proteasome activator complex subunit 3 (PSME3) LDTP04917 E171; E169  LDD0015 
Reticulocalbin-2 (RCN2) LDTP06097 E290; E299; E285  LDD0015 
Sideroflexin-5 (SFXN5) LDTP09413 E223; G222  LDD0015 
Small ribosomal subunit protein eS10 (RPS10) LDTP04111 L83; H84; D81  LDD0015 
T-complex protein 1 subunit epsilon (CCT5) LDTP04201 I102; T107; G103; D104  LDD0015 
T-complex protein 1 subunit eta (CCT7) LDTP10990 Y111; K109; Q107  LDD0015 
Transmembrane protein 256 (TMEM256) LDTP19689 F32; Q31; A27; S24  LDD0015 
Tubulin beta chain (TUBB) LDTP02205 S25; D31; E27  LDD0015 
Tubulin beta-2A chain (TUBB2A) LDTP05998 E69; D67; V66  LDD0015 
Vimentin (VIM) LDTP02297 E346; V353; A355; E348  LDD0015 
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⏷ Show the Full List of 40 Other

Full Information of The Labelling Profiles of This Probe

Qualitation: Labeling site identification
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
200 uM
Quantitative Method
Isotope tag
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Interaction Atlas ID
 LDD0015 
Download The Altas

References

1 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652