Details of the Target

General Information of Target

Target ID LDTP05977
Target Name Nascent polypeptide-associated complex subunit alpha (NACA)
Gene Name NACA
Gene ID 4666
Synonyms
Nascent polypeptide-associated complex subunit alpha; NAC-alpha; Alpha-NAC; allergen Hom s 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEID
EEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTY
IVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKD
IELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM
Target Bioclass
Other
Family
NAC-alpha family
Subcellular location
Cytoplasm
Function
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
Uniprot ID
Q13765
Ensemble ID
ENST00000356769.7
HGNC ID
HGNC:7629

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-9
 Probe Info  		8.05  LDD0393  [1]
C-Sul
 Probe Info  		8.27  LDD0066  [2]
TH211
 Probe Info  		Y112(20.00)  LDD0257  [3]
TH216
 Probe Info  		Y112(20.00)  LDD0259  [3]
OPA-S-S-alkyne
 Probe Info  		K82(3.23)  LDD3494  [4]
Probe 1
 Probe Info  		Y120(33.59)  LDD3495  [5]
P11
 Probe Info  		5.69  LDD0201  [6]
HHS-482
 Probe Info  		Y120(0.29)  LDD0285  [7]
HHS-475
 Probe Info  		Y112(0.84); Y120(0.94)  LDD0264  [8]
HHS-465
 Probe Info  		Y120(10.00)  LDD2237  [9]
ATP probe
 Probe Info  		K142(0.00); K144(0.00); K82(0.00); K100(0.00)  LDD0199  [10]
NHS
 Probe Info  		K108(0.00); K142(0.00); K100(0.00)  LDD0010  [11]
SF
 Probe Info  		Y112(0.00); K100(0.00); Y120(0.00); K113(0.00)  LDD0028  [12]
STPyne
 Probe Info  		N.A.  LDD0009  [11]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe3
 Probe Info  		13.09  LDD0464  [13]
PARPYnD
 Probe Info  		0.56  LDD0376  [14]
Diazir
 Probe Info  		N.A.  LDD0011  [11]
LD-F
 Probe Info  		S132(0.00); D130(0.00); L131(0.00)  LDD0015  [15]
DA-2
 Probe Info  		N.A.  LDD0073  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0147  AZ0108 MDA-MB-468 0.56  LDD0376  [14]
 LDCM0088  C45 HEK-293T 5.69  LDD0201  [6]
 LDCM0116  HHS-0101 DM93 Y112(0.84); Y120(0.94)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y112(0.71); Y120(1.06)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y112(0.90); Y120(1.32)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y112(0.84); Y120(1.29)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y112(0.74); Y120(0.98)  LDD0268  [8]
 LDCM0123  JWB131 DM93 Y120(0.29)  LDD0285  [7]
 LDCM0124  JWB142 DM93 Y120(0.86)  LDD0286  [7]
 LDCM0125  JWB146 DM93 Y112(0.09); Y120(0.85)  LDD0287  [7]
 LDCM0126  JWB150 DM93 Y112(0.14); Y120(2.36)  LDD0288  [7]
 LDCM0127  JWB152 DM93 Y112(0.26); Y120(1.10)  LDD0289  [7]
 LDCM0128  JWB198 DM93 Y112(0.07); Y120(0.34)  LDD0290  [7]
 LDCM0129  JWB202 DM93 Y112(0.09)  LDD0291  [7]
 LDCM0130  JWB211 DM93 Y112(0.18)  LDD0292  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-taxilin (TXLNA) Taxilin family P40222

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
7 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
14 Structure-Guided Design and In-Cell Target Profiling of a Cell-Active Target Engagement Probe for PARP Inhibitors. ACS Chem Biol. 2020 Feb 21;15(2):325-333. doi: 10.1021/acschembio.9b00963. Epub 2020 Feb 10.
15 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652
16 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.