Details of the Target

General Information of Target

Target ID LDTP11844
Target Name Protein spinster homolog 1 (SPNS1)
Gene Name SPNS1
Gene ID 83985
Synonyms
SPIN1; Protein spinster homolog 1; HSpin1; SPNS1; Spinster-like protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLPGCIFLMILLIPQVKEKFILGVEGQQLVRPKKLPLIQKRDTGHTHDDDILKTYEEELL
YEIKLNRKTLVLHLLRSREFLGSNYSETFYSMKGEAFTRHPQIMDHCFYQGSIVHEYDSA
ASISTCNGLRGFFRINDQRYLIEPVKYSDEGEHLVFKYNLRVPYGANYSCTELNFTRKTV
PGDNESEEDSKIKGIHDEKYVELFIVADDTVYRRNGHPHNKLRNRIWGMVNFVNMIYKTL
NIHVTLVGIEIWTHEDKIELYSNIETTLLRFSFWQEKILKTRKDFDHVVLLSGKWLYSHV
QGISYPGGMCLPYYSTSIIKDLLPDTNIIANRMAHQLGHNLGMQHDEFPCTCPSGKCVMD
SDGSIPALKFSKCSQNQYHQYLKDYKPTCMLNIPFPYNFHDFQFCGNKKLDEGEECDCGP
AQECTNPCCDAHTCVLKPGFTCAEGECCESCQIKKAGSICRPAKDECDFPEMCTGHSPAC
PKDQFRVNGFPCKNSEGYCFMGKCPTREDQCSELFDDEAIESHDICYKMNTKGNKFGYCK
NKENRFLPCEEKDVRCGKIYCTGGELSSLLGEDKTYHLKDPQKNATVKCKTIFLYHDSTD
IGLVASGTKCGEGMVCNNGECLNMEKVYISTNCPSQCNENPVDGHGLQCHCEEGQAPVAC
EETLHVTNITILVVVLVLVIVGIGVLILLVRYRKCIKLKQVQSPPTETLGVENKGYFGDE
QQIRTEPILPEIHFLNKPASKDSRGIADPNQSAK
Target Bioclass
Transporter and channel
Family
Major facilitator superfamily, Spinster (TC 2.A.1.49) family
Subcellular location
Lysosome membrane
Function
Plays a critical role in the phospholipid salvage pathway from lysosomes to the cytosol. Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids, which can then be reacylated by acyltransferases in the endoplasmic reticulum to form phospholipids. Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), can also transport lysophosphatidylglycerol (LPG), but not other anionic lysophospholipids, sphingosine, nor sphingomyelin. Transports lysophospholipids with saturated, monounsaturated, and polyunsaturated fatty acids, such as 1-hexadecanoyl-sn-glycero-3-phosphocholine, 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine and 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine, respectively. Can also transport lysoplasmalogen (LPC with a fatty alcohol) such as 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine. Lysosomal LPC could function as intracellular signaling messenger. Essential player in lysosomal homeostasis. Crucial for cell survival under conditions of nutrient limitation. May be involved in necrotic or autophagic cell death.
Uniprot ID
Q9H2V7
Ensemble ID
ENST00000311008.16
HGNC ID
HGNC:30621

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ICC12 Deletion: p.F115AfsTer23 .
JURKAT SNV: p.A282V .
LOXIMVI SNV: p.S319F .
LS123 SNV: p.D488A .
LS180 SNV: p.R157W .
MFE296 SNV: p.A176T .
MOLT4 SNV: p.F462S .
NCIH2286 SNV: p.W260C .
SNU1 SNV: p.G314R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C96(1.61)  LDD3442  [1]
AOyne
 Probe Info  		15.00  LDD0443  [2]
PAL-AfBPP Probe
Click To Hide/Show 61 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		12.64  LDD1714  [3]
C022
 Probe Info  		11.96  LDD1728  [3]
C049
 Probe Info  		6.92  LDD1747  [3]
C056
 Probe Info  		27.28  LDD1753  [3]
C063
 Probe Info  		28.84  LDD1760  [3]
C089
 Probe Info  		5.50  LDD1781  [3]
C091
 Probe Info  		22.16  LDD1782  [3]
C092
 Probe Info  		40.22  LDD1783  [3]
C094
 Probe Info  		29.65  LDD1785  [3]
C106
 Probe Info  		38.32  LDD1793  [3]
C107
 Probe Info  		6.19  LDD1794  [3]
C108
 Probe Info  		13.00  LDD1795  [3]
C140
 Probe Info  		7.73  LDD1822  [3]
C143
 Probe Info  		16.00  LDD1825  [3]
C145
 Probe Info  		8.40  LDD1827  [3]
C147
 Probe Info  		7.78  LDD1829  [3]
C153
 Probe Info  		25.46  LDD1834  [3]
C159
 Probe Info  		14.42  LDD1839  [3]
C160
 Probe Info  		11.16  LDD1840  [3]
C161
 Probe Info  		26.17  LDD1841  [3]
C178
 Probe Info  		15.03  LDD1857  [3]
C201
 Probe Info  		28.25  LDD1877  [3]
C206
 Probe Info  		16.34  LDD1881  [3]
C225
 Probe Info  		6.63  LDD1898  [3]
C226
 Probe Info  		6.41  LDD1899  [3]
C228
 Probe Info  		19.16  LDD1901  [3]
C264
 Probe Info  		29.65  LDD1935  [3]
C265
 Probe Info  		16.91  LDD1936  [3]
C286
 Probe Info  		7.26  LDD1956  [3]
C287
 Probe Info  		33.82  LDD1957  [3]
C288
 Probe Info  		14.32  LDD1958  [3]
C289
 Probe Info  		38.05  LDD1959  [3]
C290
 Probe Info  		5.24  LDD1960  [3]
C296
 Probe Info  		15.14  LDD1966  [3]
C305
 Probe Info  		28.25  LDD1974  [3]
C314
 Probe Info  		15.56  LDD1981  [3]
C355
 Probe Info  		42.52  LDD2016  [3]
C356
 Probe Info  		14.32  LDD2017  [3]
C357
 Probe Info  		5.78  LDD2018  [3]
C362
 Probe Info  		69.07  LDD2023  [3]
C363
 Probe Info  		48.84  LDD2024  [3]
C364
 Probe Info  		43.11  LDD2025  [3]
C366
 Probe Info  		5.98  LDD2027  [3]
C367
 Probe Info  		5.06  LDD2028  [3]
C378
 Probe Info  		7.46  LDD2037  [3]
C382
 Probe Info  		16.22  LDD2041  [3]
C399
 Probe Info  		8.88  LDD2058  [3]
C403
 Probe Info  		27.47  LDD2061  [3]
C420
 Probe Info  		13.64  LDD2075  [3]
C429
 Probe Info  		18.13  LDD2084  [3]
C431
 Probe Info  		16.56  LDD2086  [3]
C433
 Probe Info  		6.06  LDD2088  [3]
FFF probe11
 Probe Info  		19.75  LDD0471  [4]
FFF probe13
 Probe Info  		20.00  LDD0475  [4]
FFF probe14
 Probe Info  		20.00  LDD0477  [4]
FFF probe15
 Probe Info  		17.00  LDD0478  [4]
FFF probe4
 Probe Info  		20.00  LDD0466  [4]
JN0003
 Probe Info  		20.00  LDD0469  [4]
Alk-rapa
 Probe Info  		3.35  LDD0213  [5]
LD-F
 Probe Info  		L429(0.00); G433(0.00); D431(0.00)  LDD0015  [6]
OEA-DA
 Probe Info  		20.00  LDD0046  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C376(1.00)  LDD1510  [8]
 LDCM0270  AC15 HEK-293T C376(1.41)  LDD1513  [8]
 LDCM0280  AC20 HEK-293T C376(0.85)  LDD1519  [8]
 LDCM0283  AC23 HEK-293T C376(1.09)  LDD1522  [8]
 LDCM0288  AC28 HEK-293T C376(1.23)  LDD1527  [8]
 LDCM0292  AC31 HEK-293T C376(1.08)  LDD1531  [8]
 LDCM0297  AC36 HEK-293T C376(0.90)  LDD1536  [8]
 LDCM0300  AC39 HEK-293T C376(1.14)  LDD1539  [8]
 LDCM0301  AC4 HEK-293T C376(0.89)  LDD1540  [8]
 LDCM0306  AC44 HEK-293T C376(0.82)  LDD1545  [8]
 LDCM0309  AC47 HEK-293T C376(0.99)  LDD1548  [8]
 LDCM0315  AC52 HEK-293T C376(0.83)  LDD1554  [8]
 LDCM0318  AC55 HEK-293T C376(1.14)  LDD1557  [8]
 LDCM0324  AC60 HEK-293T C376(1.13)  LDD1563  [8]
 LDCM0327  AC63 HEK-293T C376(1.12)  LDD1566  [8]
 LDCM0334  AC7 HEK-293T C376(1.04)  LDD1568  [8]
 LDCM0379  CL11 HEK-293T C376(1.47)  LDD1583  [8]
 LDCM0408  CL20 HEK-293T C376(1.21)  LDD1612  [8]
 LDCM0411  CL23 HEK-293T C376(1.08)  LDD1615  [8]
 LDCM0421  CL32 HEK-293T C376(1.10)  LDD1625  [8]
 LDCM0424  CL35 HEK-293T C376(0.92)  LDD1628  [8]
 LDCM0434  CL44 HEK-293T C376(0.81)  LDD1638  [8]
 LDCM0437  CL47 HEK-293T C376(1.15)  LDD1641  [8]
 LDCM0447  CL56 HEK-293T C376(1.15)  LDD1650  [8]
 LDCM0450  CL59 HEK-293T C376(0.99)  LDD1653  [8]
 LDCM0460  CL68 HEK-293T C376(0.97)  LDD1663  [8]
 LDCM0464  CL71 HEK-293T C376(1.23)  LDD1667  [8]
 LDCM0473  CL8 HEK-293T C376(1.19)  LDD1676  [8]
 LDCM0474  CL80 HEK-293T C376(0.82)  LDD1677  [8]
 LDCM0477  CL83 HEK-293T C376(1.38)  LDD1680  [8]
 LDCM0487  CL92 HEK-293T C376(1.11)  LDD1690  [8]
 LDCM0490  CL95 HEK-293T C376(1.26)  LDD1693  [8]
 LDCM0022  KB02 HuH-1 C96(1.42)  LDD2374  [1]
 LDCM0023  KB03 HuH-1 C96(1.84)  LDD2791  [1]
 LDCM0024  KB05 SNU-423 C96(1.61)  LDD3442  [1]
 LDCM0090  Rapamycin JHH-7 3.35  LDD0213  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bcl-2-like protein 1 (BCL2L1) Bcl-2 family Q07817

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
4 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
5 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.
6 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652
7 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402