Probe Name	FFF probe15					Probe Info
Probe concentration	Probe:200uM; negative probe:200uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	SILAC
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP10032	7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B)					20.0
LDTP00968	Acyl-CoA (8-3)-desaturase (FADS1)					20.0
LDTP01699	Acyl-CoA 6-desaturase (FADS2)					20.0
LDTP06618	ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)					20.0
LDTP02254	ATP-dependent translocase ABCB1 (ABCB1)					20.0
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					20.0
LDTP05171	Dermcidin (DCD)					20.0
LDTP01769	Dihydrofolate reductase (DHFR)					20.0
LDTP06150	DNA replication licensing factor MCM6 (MCM6)					20.0
LDTP11259	Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (ALG12)					20.0
LDTP12279	Endoplasmic reticulum transmembrane helix translocase (ATP13A1)					20.0
LDTP09200	FAD synthase (FLAD1)					20.0
LDTP02651	Gamma-interferon-inducible lysosomal thiol reductase (IFI30)					20.0
LDTP03540	Heme oxygenase 2 (HMOX2)					20.0
LDTP02005	Lysosomal acid glucosylceramidase (GBA1)					20.0
LDTP02486	Lysosomal alpha-glucosidase (GAA)					20.0
LDTP02505	Lysosomal protective protein (CTSA)					20.0
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					20.0
LDTP06638	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2)					20.0
LDTP08859	Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4)					20.0
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					20.0
LDTP15422	Prostaglandin reductase 3 (PTGR3)					20.0
LDTP00877	Protein SCO2 homolog, mitochondrial (SCO2)					20.0
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					20.0
LDTP06333	Serum paraoxonase/arylesterase 2 (PON2)					20.0
LDTP00544	Sphingolipid delta(4)-desaturase DES1 (DEGS1)					20.0
LDTP01613	Sphingosine-1-phosphate lyase 1 (SGPL1)					20.0
LDTP03842	Squalene synthase (FDFT1)					20.0
LDTP00344	Stearoyl-CoA desaturase (SCD)					20.0
LDTP04251	Elongation factor Tu, mitochondrial (TUFM)					19.1
LDTP12628	Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10)					18.8
LDTP02198	Cathepsin D (CTSD)					17.6
LDTP13620	Lysosomal thioesterase PPT2 (PPT2)					16.7
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					16.6
LDTP05029	Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A)					16.6
LDTP02602	Histidine--tRNA ligase, cytoplasmic (HARS1)					16.6
LDTP04634	Alpha-N-acetylglucosaminidase (NAGLU)					16.2
LDTP12253	Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)					15.7
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					15.5
LDTP12048	Complex I assembly factor ACAD9, mitochondrial (ACAD9)					14.7
LDTP14276	NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9)					14.2
LDTP04051	Nicotinamide phosphoribosyltransferase (NAMPT)					14.2
LDTP02141	Alpha-galactosidase A (GLA)					14.1
LDTP02169	Beta-hexosaminidase subunit alpha (HEXA)					13.8
LDTP06270	Signal peptidase complex subunit 2 (SPCS2)					13.5
LDTP07154	ATPase family AAA domain-containing protein 3B (ATAD3B)					13.3
LDTP03680	DNA replication licensing factor MCM4 (MCM4)					13.3
LDTP05724	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)					12.8
LDTP10101	Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10)					12.7
LDTP02565	Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM)					12.4
LDTP03433	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1)					12.4
LDTP05862	NAD(P) transhydrogenase, mitochondrial (NNT)					12.2
LDTP00950	Dynamin-like 120 kDa protein, mitochondrial (OPA1)					12.1
LDTP05301	Hydroxymethylglutaryl-CoA synthase, cytoplasmic (HMGCS1)					12.1
LDTP02231	Tyrosine-protein kinase Yes (YES1)					12.1
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					12.0
LDTP03145	Catechol O-methyltransferase (COMT)					11.5
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					11.4
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					10.9
LDTP08799	Lysophosphatidylserine lipase ABHD12 (ABHD12)					10.8
LDTP11081	NAD-capped RNA hydrolase NUDT12 (NUDT12)					10.8
LDTP03418	Proteasome subunit beta type-6 (PSMB6)					10.6
LDTP00428	Tripeptidyl-peptidase 1 (TPP1)					10.5
LDTP06901	Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12)					10.1
LDTP11874	Thioredoxin-related transmembrane protein 1 (TMX1)					9.9
LDTP12233	Helicase MOV-10 (MOV10)					9.8
LDTP02837	Beta-galactosidase (GLB1)					9.7
LDTP01328	CAAX prenyl protease 1 homolog (ZMPSTE24)					9.6
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					9.4
LDTP11215	Plasma alpha-L-fucosidase (FUCA2)					9.2
LDTP06905	Acylglycerol kinase, mitochondrial (AGK)					9.0
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					9.0
LDTP12681	ATPase family AAA domain-containing protein 3A (ATAD3A)					8.9
LDTP06375	Delta(24)-sterol reductase (DHCR24)					8.8
LDTP09251	Atlastin-2 (ATL2)					8.7
LDTP02223	Cathepsin B (CTSB)					8.7
LDTP00988	Protein O-GlcNAcase (OGA)					8.7
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					8.5
LDTP04572	Dipeptidyl peptidase 1 (CTSC)					8.5
LDTP06720	Peroxisomal leader peptide-processing protease (TYSND1)					8.3
LDTP00697	Cytochrome b5 type B (CYB5B)					8.0
LDTP01768	Glutamate dehydrogenase 1, mitochondrial (GLUD1)					7.9
LDTP01773	Cytochrome c oxidase subunit 2 (MT-CO2)					7.8
LDTP14213	Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (ALG6)					7.6
LDTP00486	Cytochrome b-c1 complex subunit 8 (UQCRQ)					7.5
LDTP03189	Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2)					7.5
LDTP12867	Very-long-chain enoyl-CoA reductase (TECR)					7.4
LDTP05632	Mitochondrial-processing peptidase subunit alpha (PMPCA)					7.3
LDTP09188	Lysophosphatidylcholine acyltransferase 1 (LPCAT1)					7.2
LDTP04120	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A)					7.2
LDTP11437	Peroxisomal trans-2-enoyl-CoA reductase (PECR)					6.8
LDTP03139	Succinate dehydrogenase iron-sulfur subunit, mitochondrial (SDHB)					6.8
LDTP05128	Glutathione S-transferase omega-1 (GSTO1)					6.8
LDTP04284	Proteasome subunit beta type-3 (PSMB3)					6.6
LDTP03060	Proteasome subunit beta type-1 (PSMB1)					6.6
LDTP02848	NADPH--cytochrome P450 reductase (POR)					6.5
LDTP03419	Proteasome subunit beta type-5 (PSMB5)					6.5
LDTP06104	Peptidyl-prolyl cis-trans isomerase FKBP8 (FKBP8)					6.4
LDTP09388	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B (STT3B)					6.4
LDTP04471	Ribosomal protein S6 kinase alpha-3 (RPS6KA3)					6.4
LDTP01241	Bis(monoacylglycero)phosphate synthase CLN5 (CLN5)					6.3
LDTP11209	Phosphatidylinositol 4-kinase type 2-alpha (PI4K2A)					6.1
LDTP03753	Cystathionine beta-synthase (CBS)					6.0
LDTP10888	Legumain (LGMN)					5.9
LDTP00597	Serine palmitoyltransferase 1 (SPTLC1)					5.9
LDTP00783	Beta-1,4-glucuronyltransferase 1 (B4GAT1)					5.9
LDTP02743	Insulin-degrading enzyme (IDE)					5.8
LDTP07504	Lysophospholipid acyltransferase 5 (LPCAT3)					5.8
LDTP04581	Holocytochrome c-type synthase (HCCS)					5.7
LDTP12020	Histone-lysine N-methyltransferase SMYD3 (SMYD3)					5.7
LDTP04327	Cytosolic purine 5'-nucleotidase (NT5C2)					5.7
LDTP09962	Ubiquitin carboxyl-terminal hydrolase 7 (USP7)					5.7
LDTP01168	NADH dehydrogenase iron-sulfur protein 2, mitochondrial (NDUFS2)					5.6
LDTP07003	Presequence protease, mitochondrial (PITRM1)					5.6
LDTP03689	Serine hydroxymethyltransferase, mitochondrial (SHMT2)					5.5
LDTP07362	Atlastin-3 (ATL3)					5.5
LDTP03525	Cell division cycle protein 27 homolog (CDC27)					5.5
LDTP08596	Mitochondrial Rho GTPase 2 (RHOT2)					5.5
LDTP13744	Dynamin-3 (DNM3)					5.5
LDTP02860	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2)					5.5
LDTP09886	Gamma-glutamyl hydrolase (GGH)					5.4
LDTP10862	Proteasome subunit beta type-7 (PSMB7)					5.4
LDTP04962	26S proteasome regulatory subunit 4 (PSMC1)					5.4
LDTP00032	2-hydroxyacyl-CoA lyase 2 (ILVBL)					5.4
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					5.4
LDTP12954	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 (HACD3)					5.3
LDTP02216	Beta-hexosaminidase subunit beta (HEXB)					5.3
LDTP01687	Apoptosis-inducing factor 1, mitochondrial (AIFM1)					5.3
LDTP10318	Ubiquitin thioesterase OTUB1 (OTUB1)					5.2
LDTP11321	Acetyl-CoA acetyltransferase, cytosolic (ACAT2)					5.2
LDTP04437	Aldehyde dehydrogenase family 3 member A2 (ALDH3A2)					5.2
LDTP02385	Leukotriene A-4 hydrolase (LTA4H)					5.1
LDTP11187	COP9 signalosome complex subunit 4 (COPS4)					5.1
LDTP03912	Trifunctional enzyme subunit alpha, mitochondrial (HADHA)					5.1

Target ID	Target Name					Binding Ratio
LDTP11245	Derlin-1 (DERL1)					20.0
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					20.0
LDTP10513	Exocyst complex component 2 (EXOC2)					20.0
LDTP01366	Flotillin-1 (FLOT1)					20.0
LDTP05080	Hemoglobin subunit beta (HBB)					20.0
LDTP05900	Metaxin-1 (MTX1)					20.0
LDTP07472	Mitochondrial adenyl nucleotide antiporter SLC25A24 (SLC25A24)					20.0
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					20.0
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					20.0
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					20.0
LDTP00541	NPC intracellular cholesterol transporter 1 (NPC1)					20.0
LDTP02215	Prosaposin (PSAP)					20.0
LDTP07236	Protein GPR107 (GPR107)					20.0
LDTP13164	Protein sel-1 homolog 1 (SEL1L)					20.0
LDTP14084	Sorting and assembly machinery component 50 homolog (SAMM50)					20.0
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					20.0
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					20.0
LDTP11615	Protein tweety homolog 3 (TTYH3)					19.4
LDTP13174	Mitochondrial dicarboxylate carrier (SLC25A10)					18.3
LDTP15948	Transmembrane protein 126A (TMEM126A)					18.2
LDTP15481	Transmembrane protein 87A (TMEM87A)					17.7
LDTP18448	Transmembrane 9 superfamily member 3 (TM9SF3)					17.4
LDTP11844	Protein spinster homolog 1 (SPNS1)					17.0
LDTP14952	Mpv17-like protein 2 (MPV17L2)					16.6
LDTP06426	Translocating chain-associated membrane protein 1 (TRAM1)					15.9
LDTP06308	Mitochondrial inner membrane protein OXA1L (OXA1L)					15.7
LDTP09968	V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1)					15.0
LDTP07156	Protein wntless homolog (WLS)					14.3
LDTP01301	Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial (SLC25A12)					14.2
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					14.2
LDTP01745	Putative lipid scramblase CLPTM1 (CLPTM1)					13.2
LDTP11213	Nucleoporin NDC1 (NDC1)					12.9
LDTP03104	V-type proton ATPase subunit B, brain isoform (ATP6V1B2)					12.9
LDTP04553	Tricarboxylate transport protein, mitochondrial (SLC25A1)					12.2
LDTP04501	Importin subunit alpha-1 (KPNA2)					12.1
LDTP13393	Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial (SLC25A13)					11.8
LDTP00310	Syntenin-1 (SDCBP)					11.6
LDTP00562	Membrane-associated progesterone receptor component 2 (PGRMC2)					11.5
LDTP05511	Cytoskeleton-associated protein 4 (CKAP4)					11.2
LDTP00434	Transportin-2 (TNPO2)					10.7
LDTP10036	BOS complex subunit NCLN (NCLN)					9.7
LDTP03559	High affinity cationic amino acid transporter 1 (SLC7A1)					9.5
LDTP00477	Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23)					9.1
LDTP12042	Proton-activated chloride channel (PACC1)					8.8
LDTP04707	Protein SEC13 homolog (SEC13)					8.7
LDTP00592	Surfeit locus protein 4 (SURF4)					7.8
LDTP11531	Oxysterol-binding protein-related protein 8 (OSBPL8)					7.8
LDTP14235	Mitochondrial carrier homolog 2 (MTCH2)					7.7
LDTP04932	Protein transport protein Sec61 subunit alpha isoform 1 (SEC61A1)					7.6
LDTP02655	Lysosome-associated membrane glycoprotein 2 (LAMP2)					7.5
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					6.6
LDTP01525	Mitochondrial proton/calcium exchanger protein (LETM1)					6.5
LDTP08960	ER membrane protein complex subunit 1 (EMC1)					6.5
LDTP06660	MICOS complex subunit MIC60 (IMMT)					6.4
LDTP13243	Translocation protein SEC63 homolog (SEC63)					6.4
LDTP01601	Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1)					6.3
LDTP13824	Voltage-dependent anion-selective channel protein 3 (VDAC3)					6.3
LDTP05690	Vesicular integral-membrane protein VIP36 (LMAN2)					6.2
LDTP03810	ATP synthase subunit gamma, mitochondrial (ATP5F1C)					5.8
LDTP13969	Transmembrane emp24 domain-containing protein 7 (TMED7)					5.8
LDTP11934	EH domain-containing protein 1 (EHD1)					5.7
LDTP10059	PAT complex subunit CCDC47 (CCDC47)					5.4
LDTP10663	Importin-9 (IPO9)					5.3
LDTP04293	Transmembrane emp24 domain-containing protein 10 (TMED10)					5.1
LDTP03405	Calnexin (CANX)					5.1
LDTP02071	Amyloid-beta precursor protein (APP)					5.1
LDTP12090	Sideroflexin-1 (SFXN1)					5.0

Target ID	Target Name					Binding Ratio
LDTP00286	Homeobox protein Meis1 (MEIS1)					18.2
LDTP00425	Homeobox protein Meis2 (MEIS2)					12.8

Target ID	Target Name					Binding Ratio
LDTP02491	HLA class I histocompatibility antigen, C alpha chain (HLA-C)					13.9
LDTP03772	Basigin (BSG)					5.6

Target ID	Target Name					Binding Ratio
LDTP04904	Alpha-centractin (ACTR1A)					20.0
LDTP13755	Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2)					20.0
LDTP14486	Clustered mitochondria protein homolog (CLUH)					20.0
LDTP05567	Desmocollin-1 (DSC1)					20.0
LDTP05353	Desmoglein-1 (DSG1)					20.0
LDTP03678	ER lumen protein-retaining receptor 2 (KDELR2)					20.0
LDTP15253	HEAT repeat-containing protein 3 (HEATR3)					20.0
LDTP08425	Hornerin (HRNR)					20.0
LDTP07136	Keratinocyte proline-rich protein (KPRP)					20.0
LDTP04094	Large proline-rich protein BAG6 (BAG6)					20.0
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					20.0
LDTP05375	Nucleobindin-1 (NUCB1)					20.0
LDTP05160	Nucleobindin-2 (NUCB2)					20.0
LDTP13311	Poly(U)-binding-splicing factor PUF60 (PUF60)					20.0
LDTP08130	Rab9 effector protein with kelch motifs (RABEPK)					20.0
LDTP07476	RAD50-interacting protein 1 (RINT1)					20.0
LDTP06352	Reticulocalbin-1 (RCN1)					20.0
LDTP03493	Serpin B3 (SERPINB3)					20.0
LDTP19372	Tetratricopeptide repeat protein 38 (TTC38)					20.0
LDTP08154	Large ribosomal subunit protein uL10m (MRPL10)					15.0
LDTP01652	Centrosomal protein 43 (CEP43)					14.9
LDTP00887	Calumenin (CALU)					14.8
LDTP13630	Neudesin (NENF)					14.1
LDTP00512	Protein transport protein Sec16A (SEC16A)					13.6
LDTP14792	Small ribosomal subunit protein uS9m (MRPS9)					13.4
LDTP16323	Signal recognition particle receptor subunit beta (SRPRB)					13.2
LDTP08364	Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2)					13.1
LDTP10185	FAS-associated factor 2 (FAF2)					12.3
LDTP13044	Ribosome-binding protein 1 (RRBP1)					12.3
LDTP16285	Transducin beta-like protein 2 (TBL2)					12.2
LDTP09545	Nucleolar complex protein 3 homolog (NOC3L)					11.8
LDTP00199	HCLS1-associated protein X-1 (HAX1)					11.0
LDTP00281	Golgi integral membrane protein 4 (GOLIM4)					10.8
LDTP05491	Amyloid beta precursor like protein 2 (APLP2)					10.7
LDTP16080	Protein FAM114A2 (FAM114A2)					9.9
LDTP05012	Small ribosomal subunit protein eS24 (RPS24)					9.1
LDTP13338	Vacuolar protein sorting-associated protein 51 homolog (VPS51)					8.3
LDTP14217	Lipid droplet-regulating VLDL assembly factor AUP1 (AUP1)					7.8
LDTP13430	Protein TASOR (TASOR)					7.8
LDTP10965	Nucleosome assembly protein 1-like 4 (NAP1L4)					7.6
LDTP06305	WD repeat-containing protein 43 (WDR43)					7.5
LDTP12412	Reticulon-4 (RTN4)					7.4
LDTP04684	Nucleosome assembly protein 1-like 1 (NAP1L1)					7.3
LDTP02297	Vimentin (VIM)					6.6
LDTP03614	Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3)					6.5
LDTP09787	Nicastrin (NCSTN)					6.3
LDTP13402	Dynactin subunit 4 (DCTN4)					6.1
LDTP03352	Splicing factor U2AF 65 kDa subunit (U2AF2)					6.0
LDTP07598	BRCA1-associated ATM activator 1 (BRAT1)					5.9
LDTP02756	Junction plakoglobin (JUP)					5.9
LDTP05922	Dynactin subunit 2 (DCTN2)					5.7
LDTP17065	Heterogeneous nuclear ribonucleoprotein U-like protein 2 (HNRNPUL2)					5.5
LDTP01432	Mitochondrial import receptor subunit TOM70 (TOMM70)					5.3
LDTP04290	YLP motif-containing protein 1 (YLPM1)					5.3
LDTP06097	Reticulocalbin-2 (RCN2)					5.3
LDTP04382	Kinetochore-associated protein 1 (KNTC1)					5.1

1	Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.