Details of the Target

General Information of Target

Target ID LDTP07504
Target Name Lysophospholipid acyltransferase 5 (LPCAT3)
Gene Name LPCAT3
Gene ID 10162
Synonyms
MBOAT5; OACT5; Lysophospholipid acyltransferase 5; LPLAT 5; EC 2.3.1.-; 1-acylglycerophosphocholine O-acyltransferase; EC 2.3.1.23; 1-acylglycerophosphoethanolamine O-acyltransferase; EC 2.3.1.n7; 1-acylglycerophosphoserine O-acyltransferase; EC 2.3.1.n6; Lysophosphatidylcholine acyltransferase; LPCAT; Lyso-PC acyltransferase; Lysophosphatidylcholine acyltransferase 3; Lyso-PC acyltransferase 3; Lysophosphatidylserine acyltransferase; LPSAT; Lyso-PS acyltransferase; Membrane-bound O-acyltransferase domain-containing protein 5; O-acyltransferase domain-containing protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASSAEGDEGTVVALAGVLQSGFQELSLNKLATSLGASEQALRLIISIFLGYPFALFYRH
YLFYKETYLIHLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTFCF
QMAYLLAGYYYTATGNYDIKWTMPHCVLTLKLIGLAVDYFDGGKDQNSLSSEQQKYAIRG
VPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVQGELIDIPGKIPNSIIPALKRLSLGLFY
LVGYTLLSPHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGL
GFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIFKRLKFLGNKE
LSQGLSLLFLALWHGLHSGYLVCFQMEFLIVIVERQAARLIQESPTLSKLAAITVLQPFY
YLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYKSIYFLGHIFFLSLLFILPYIHKAMVPRK
EKLKKME
Target Bioclass
Enzyme
Family
Membrane-bound acyltransferase family
Subcellular location
Endoplasmic reticulum membrane
Function
Lysophospholipid O-acyltransferase (LPLAT) that catalyzes the reacylation step of the phospholipid remodeling process also known as the Lands cycle. Catalyzes transfer of the fatty acyl chain from fatty acyl-CoA to 1-acyl lysophospholipid to form various classes of phospholipids. Converts 1-acyl lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) (LPCAT activity), 1-acyl lysophosphatidylserine (LPS) into phosphatidylserine (PS) (LPSAT activity) and 1-acyl lysophosphatidylethanolamine (LPE) into phosphatidylethanolamine (PE) (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Has higher activity for LPC acyl acceptors compared to LPEs and LPSs. Can also transfer the fatty acyl chain from fatty acyl-CoA to 1-O-alkyl lysophospholipid or 1-O-alkenyl lysophospholipid with lower efficiency. Acts as a major LPC O-acyltransferase in liver and intestine. As a component of the liver X receptor/NR1H3 or NR1H2 signaling pathway, mainly catalyzes the incorporation of arachidonate into PCs of endoplasmic reticulum (ER) membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Promotes processing of sterol regulatory protein SREBF1 in hepatocytes, likely by facilitating the translocation of SREBF1-SCAP complex from ER to the Golgi apparatus. Participates in mechanisms by which the liver X receptor/NR1H3 or NR1H2 signaling pathway counteracts lipid-induced ER stress response and inflammation. Down-regulates hepatic inflammation by limiting arachidonic acid availability for synthesis of inflammatory eicosanoids, such as prostaglandins. In enterocytes, acts as a component of a gut-brain feedback loop that coordinates dietary lipid absorption and food intake. Regulates the abundance of PCs containing linoleate and arachidonate in enterocyte membranes, enabling passive diffusion of fatty acids and cholesterol across the membrane for efficient chylomicron assembly. In the intestinal crypt, acts as a component of dietary-responsive phospholipid-cholesterol axis, regulating the biosynthesis of cholesterol and its mitogenic effects on intestinal stem cells.
Uniprot ID
Q6P1A2
Ensemble ID
ENST00000261407.9
HGNC ID
HGNC:30244

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IM95 SNV: p.T11A .
Ishikawa (Heraklio) 02 ER SNV: p.W288R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
AF-1
 Probe Info  		2.33  LDD0421  [2]
DBIA
 Probe Info  		C316(2.07)  LDD0080  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0164  [5]
IPM
 Probe Info  		N.A.  LDD0005  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
PAL-AfBPP Probe
Click To Hide/Show 21 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C141
 Probe Info  		10.27  LDD1823  [8]
C145
 Probe Info  		11.39  LDD1827  [8]
C147
 Probe Info  		7.84  LDD1829  [8]
C225
 Probe Info  		5.90  LDD1898  [8]
C228
 Probe Info  		17.15  LDD1901  [8]
C231
 Probe Info  		11.71  LDD1904  [8]
C233
 Probe Info  		8.94  LDD1906  [8]
C280
 Probe Info  		9.65  LDD1950  [8]
C285
 Probe Info  		28.25  LDD1955  [8]
C290
 Probe Info  		6.06  LDD1960  [8]
FFF probe11
 Probe Info  		7.28  LDD0471  [9]
FFF probe14
 Probe Info  		20.00  LDD0477  [9]
FFF probe15
 Probe Info  		5.78  LDD0478  [9]
FFF probe3
 Probe Info  		6.46  LDD0465  [9]
FFF probe6
 Probe Info  		12.29  LDD0467  [9]
FFF probe7
 Probe Info  		19.13  LDD0483  [9]
STS-1
 Probe Info  		N.A.  LDD0136  [10]
STS-2
 Probe Info  		N.A.  LDD0138  [10]
AX-1
 Probe Info  		3.19  LDD0441  [11]
A-DA
 Probe Info  		3.29  LDD0142  [12]
OEA-DA
 Probe Info  		20.00  LDD0046  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0176  9im MDA-MB-231 3.19  LDD0441  [11]
 LDCM0214  AC1 HCT 116 C316(0.98)  LDD0531  [3]
 LDCM0215  AC10 PaTu 8988t C316(0.77)  LDD1094  [3]
 LDCM0216  AC100 HCT 116 C316(0.75)  LDD0533  [3]
 LDCM0217  AC101 HCT 116 C316(0.72)  LDD0534  [3]
 LDCM0218  AC102 HCT 116 C316(0.65)  LDD0535  [3]
 LDCM0219  AC103 HCT 116 C316(0.61)  LDD0536  [3]
 LDCM0220  AC104 HCT 116 C316(0.71)  LDD0537  [3]
 LDCM0221  AC105 HCT 116 C316(0.60)  LDD0538  [3]
 LDCM0222  AC106 HCT 116 C316(0.62)  LDD0539  [3]
 LDCM0223  AC107 HCT 116 C316(1.09)  LDD0540  [3]
 LDCM0224  AC108 HCT 116 C316(1.02)  LDD0541  [3]
 LDCM0225  AC109 HCT 116 C316(0.94)  LDD0542  [3]
 LDCM0226  AC11 PaTu 8988t C316(0.80)  LDD1105  [3]
 LDCM0227  AC110 HCT 116 C316(0.89)  LDD0544  [3]
 LDCM0228  AC111 HCT 116 C316(0.91)  LDD0545  [3]
 LDCM0229  AC112 HCT 116 C316(1.02)  LDD0546  [3]
 LDCM0230  AC113 HCT 116 C316(0.90)  LDD0547  [3]
 LDCM0231  AC114 HCT 116 C316(0.81)  LDD0548  [3]
 LDCM0232  AC115 HCT 116 C316(0.85)  LDD0549  [3]
 LDCM0233  AC116 HCT 116 C316(0.99)  LDD0550  [3]
 LDCM0234  AC117 HCT 116 C316(1.16)  LDD0551  [3]
 LDCM0235  AC118 HCT 116 C316(1.06)  LDD0552  [3]
 LDCM0236  AC119 HCT 116 C316(1.00)  LDD0553  [3]
 LDCM0237  AC12 PaTu 8988t C316(0.79)  LDD1116  [3]
 LDCM0238  AC120 HCT 116 C316(0.77)  LDD0555  [3]
 LDCM0239  AC121 HCT 116 C316(1.09)  LDD0556  [3]
 LDCM0240  AC122 HCT 116 C316(0.81)  LDD0557  [3]
 LDCM0241  AC123 HCT 116 C316(0.95)  LDD0558  [3]
 LDCM0242  AC124 HCT 116 C316(1.07)  LDD0559  [3]
 LDCM0243  AC125 HCT 116 C316(0.79)  LDD0560  [3]
 LDCM0244  AC126 HCT 116 C316(0.90)  LDD0561  [3]
 LDCM0245  AC127 HCT 116 C316(1.12)  LDD0562  [3]
 LDCM0246  AC128 HCT 116 C316(1.09)  LDD0563  [3]
 LDCM0247  AC129 HCT 116 C316(0.89)  LDD0564  [3]
 LDCM0249  AC130 HCT 116 C316(0.92)  LDD0566  [3]
 LDCM0250  AC131 HCT 116 C316(0.87)  LDD0567  [3]
 LDCM0251  AC132 HCT 116 C316(1.10)  LDD0568  [3]
 LDCM0252  AC133 HCT 116 C316(0.98)  LDD0569  [3]
 LDCM0253  AC134 HCT 116 C316(0.96)  LDD0570  [3]
 LDCM0254  AC135 HCT 116 C316(1.09)  LDD0571  [3]
 LDCM0255  AC136 HCT 116 C316(1.10)  LDD0572  [3]
 LDCM0256  AC137 HCT 116 C316(1.16)  LDD0573  [3]
 LDCM0257  AC138 HCT 116 C316(1.13)  LDD0574  [3]
 LDCM0258  AC139 HCT 116 C316(1.04)  LDD0575  [3]
 LDCM0259  AC14 PaTu 8988t C316(0.90)  LDD1138  [3]
 LDCM0260  AC140 HCT 116 C316(1.04)  LDD0577  [3]
 LDCM0261  AC141 HCT 116 C316(1.06)  LDD0578  [3]
 LDCM0262  AC142 HCT 116 C316(1.12)  LDD0579  [3]
 LDCM0263  AC143 HCT 116 C316(0.96)  LDD0580  [3]
 LDCM0264  AC144 HCT 116 C316(0.66)  LDD0581  [3]
 LDCM0265  AC145 HCT 116 C316(0.73)  LDD0582  [3]
 LDCM0266  AC146 HCT 116 C316(0.78)  LDD0583  [3]
 LDCM0267  AC147 HCT 116 C316(0.76)  LDD0584  [3]
 LDCM0268  AC148 HCT 116 C316(0.86)  LDD0585  [3]
 LDCM0269  AC149 HCT 116 C316(0.70)  LDD0586  [3]
 LDCM0270  AC15 PaTu 8988t C316(0.80)  LDD1149  [3]
 LDCM0271  AC150 HCT 116 C316(0.78)  LDD0588  [3]
 LDCM0272  AC151 HCT 116 C316(0.69)  LDD0589  [3]
 LDCM0273  AC152 HCT 116 C316(0.71)  LDD0590  [3]
 LDCM0274  AC153 HCT 116 C316(0.79)  LDD0591  [3]
 LDCM0621  AC154 HCT 116 C316(0.72)  LDD2158  [3]
 LDCM0622  AC155 HCT 116 C316(0.65)  LDD2159  [3]
 LDCM0623  AC156 HCT 116 C316(0.77)  LDD2160  [3]
 LDCM0624  AC157 HCT 116 C316(0.81)  LDD2161  [3]
 LDCM0276  AC17 HCT 116 C316(1.05)  LDD0593  [3]
 LDCM0277  AC18 HCT 116 C316(0.73)  LDD0594  [3]
 LDCM0278  AC19 HCT 116 C316(0.80)  LDD0595  [3]
 LDCM0279  AC2 HCT 116 C316(0.95)  LDD0596  [3]
 LDCM0280  AC20 HCT 116 C316(0.86)  LDD0597  [3]
 LDCM0281  AC21 HCT 116 C316(0.85)  LDD0598  [3]
 LDCM0282  AC22 HCT 116 C316(1.10)  LDD0599  [3]
 LDCM0283  AC23 HCT 116 C316(0.94)  LDD0600  [3]
 LDCM0284  AC24 HCT 116 C316(0.93)  LDD0601  [3]
 LDCM0285  AC25 HCT 116 C316(0.92)  LDD0602  [3]
 LDCM0286  AC26 HCT 116 C316(0.90)  LDD0603  [3]
 LDCM0287  AC27 HCT 116 C316(0.67)  LDD0604  [3]
 LDCM0288  AC28 HCT 116 C316(0.94)  LDD0605  [3]
 LDCM0289  AC29 HCT 116 C316(0.74)  LDD0606  [3]
 LDCM0290  AC3 HCT 116 C316(1.03)  LDD0607  [3]
 LDCM0291  AC30 HCT 116 C316(0.72)  LDD0608  [3]
 LDCM0292  AC31 HCT 116 C316(0.75)  LDD0609  [3]
 LDCM0293  AC32 HCT 116 C316(0.73)  LDD0610  [3]
 LDCM0294  AC33 HCT 116 C316(0.71)  LDD0611  [3]
 LDCM0295  AC34 HCT 116 C316(0.68)  LDD0612  [3]
 LDCM0296  AC35 HCT 116 C316(1.30)  LDD0613  [3]
 LDCM0297  AC36 HCT 116 C316(1.26)  LDD0614  [3]
 LDCM0298  AC37 HCT 116 C316(1.18)  LDD0615  [3]
 LDCM0299  AC38 HCT 116 C316(1.10)  LDD0616  [3]
 LDCM0300  AC39 HCT 116 C316(1.04)  LDD0617  [3]
 LDCM0301  AC4 HCT 116 C316(0.97)  LDD0618  [3]
 LDCM0302  AC40 HCT 116 C316(1.03)  LDD0619  [3]
 LDCM0303  AC41 HCT 116 C316(1.14)  LDD0620  [3]
 LDCM0304  AC42 HCT 116 C316(1.05)  LDD0621  [3]
 LDCM0305  AC43 HCT 116 C316(1.10)  LDD0622  [3]
 LDCM0306  AC44 HCT 116 C316(1.03)  LDD0623  [3]
 LDCM0307  AC45 HCT 116 C316(1.03)  LDD0624  [3]
 LDCM0308  AC46 HCT 116 C316(1.06)  LDD0625  [3]
 LDCM0309  AC47 HCT 116 C316(0.93)  LDD0626  [3]
 LDCM0310  AC48 HCT 116 C316(0.89)  LDD0627  [3]
 LDCM0311  AC49 HCT 116 C316(0.99)  LDD0628  [3]
 LDCM0312  AC5 HCT 116 C316(1.05)  LDD0629  [3]
 LDCM0313  AC50 HCT 116 C316(0.92)  LDD0630  [3]
 LDCM0314  AC51 HCT 116 C316(0.92)  LDD0631  [3]
 LDCM0315  AC52 HCT 116 C316(0.86)  LDD0632  [3]
 LDCM0316  AC53 HCT 116 C316(0.91)  LDD0633  [3]
 LDCM0317  AC54 HCT 116 C316(1.03)  LDD0634  [3]
 LDCM0318  AC55 HCT 116 C316(1.02)  LDD0635  [3]
 LDCM0319  AC56 HCT 116 C316(1.06)  LDD0636  [3]
 LDCM0320  AC57 HCT 116 C316(1.11)  LDD0637  [3]
 LDCM0321  AC58 HCT 116 C316(1.05)  LDD0638  [3]
 LDCM0322  AC59 HCT 116 C316(0.90)  LDD0639  [3]
 LDCM0323  AC6 PaTu 8988t C316(0.79)  LDD1202  [3]
 LDCM0324  AC60 HCT 116 C316(0.92)  LDD0641  [3]
 LDCM0325  AC61 HCT 116 C316(1.02)  LDD0642  [3]
 LDCM0326  AC62 HCT 116 C316(0.97)  LDD0643  [3]
 LDCM0327  AC63 HCT 116 C316(0.98)  LDD0644  [3]
 LDCM0328  AC64 HCT 116 C316(0.97)  LDD0645  [3]
 LDCM0329  AC65 HCT 116 C316(0.95)  LDD0646  [3]
 LDCM0330  AC66 HCT 116 C316(1.01)  LDD0647  [3]
 LDCM0331  AC67 HCT 116 C316(1.04)  LDD0648  [3]
 LDCM0332  AC68 HCT 116 C316(0.97)  LDD0649  [3]
 LDCM0333  AC69 HCT 116 C316(0.95)  LDD0650  [3]
 LDCM0334  AC7 PaTu 8988t C316(1.00)  LDD1213  [3]
 LDCM0335  AC70 HCT 116 C316(0.91)  LDD0652  [3]
 LDCM0336  AC71 HCT 116 C316(0.98)  LDD0653  [3]
 LDCM0337  AC72 HCT 116 C316(0.87)  LDD0654  [3]
 LDCM0338  AC73 HCT 116 C316(0.98)  LDD0655  [3]
 LDCM0339  AC74 HCT 116 C316(1.08)  LDD0656  [3]
 LDCM0340  AC75 HCT 116 C316(1.14)  LDD0657  [3]
 LDCM0341  AC76 HCT 116 C316(1.00)  LDD0658  [3]
 LDCM0342  AC77 HCT 116 C316(0.86)  LDD0659  [3]
 LDCM0343  AC78 HCT 116 C316(0.89)  LDD0660  [3]
 LDCM0344  AC79 HCT 116 C316(1.14)  LDD0661  [3]
 LDCM0345  AC8 PaTu 8988t C316(0.92)  LDD1224  [3]
 LDCM0346  AC80 HCT 116 C316(0.97)  LDD0663  [3]
 LDCM0347  AC81 HCT 116 C316(1.28)  LDD0664  [3]
 LDCM0348  AC82 HCT 116 C316(1.29)  LDD0665  [3]
 LDCM0349  AC83 HCT 116 C316(1.09)  LDD0666  [3]
 LDCM0350  AC84 HCT 116 C316(0.99)  LDD0667  [3]
 LDCM0351  AC85 HCT 116 C316(1.07)  LDD0668  [3]
 LDCM0352  AC86 HCT 116 C316(1.04)  LDD0669  [3]
 LDCM0353  AC87 HCT 116 C316(1.07)  LDD0670  [3]
 LDCM0354  AC88 HCT 116 C316(1.04)  LDD0671  [3]
 LDCM0355  AC89 HCT 116 C316(0.98)  LDD0672  [3]
 LDCM0357  AC90 HCT 116 C316(1.11)  LDD0674  [3]
 LDCM0358  AC91 HCT 116 C316(1.06)  LDD0675  [3]
 LDCM0359  AC92 HCT 116 C316(1.10)  LDD0676  [3]
 LDCM0360  AC93 HCT 116 C316(0.96)  LDD0677  [3]
 LDCM0361  AC94 HCT 116 C316(1.45)  LDD0678  [3]
 LDCM0362  AC95 HCT 116 C316(1.05)  LDD0679  [3]
 LDCM0363  AC96 HCT 116 C316(1.20)  LDD0680  [3]
 LDCM0364  AC97 HCT 116 C316(1.05)  LDD0681  [3]
 LDCM0365  AC98 HCT 116 C316(0.68)  LDD0682  [3]
 LDCM0366  AC99 HCT 116 C316(0.70)  LDD0683  [3]
 LDCM0166  Afatinib A431 2.33  LDD0421  [2]
 LDCM0248  AKOS034007472 PaTu 8988t C316(0.77)  LDD1127  [3]
 LDCM0356  AKOS034007680 PaTu 8988t C316(0.79)  LDD1235  [3]
 LDCM0275  AKOS034007705 PaTu 8988t C316(0.76)  LDD1154  [3]
 LDCM0083  Avasimibe A-549 2.05  LDD0143  [12]
 LDCM0367  CL1 HCT 116 C316(1.34)  LDD0684  [3]
 LDCM0368  CL10 HCT 116 C316(1.05)  LDD0685  [3]
 LDCM0369  CL100 HCT 116 C316(0.96)  LDD0686  [3]
 LDCM0370  CL101 PaTu 8988t C316(0.79)  LDD1249  [3]
 LDCM0371  CL102 PaTu 8988t C316(0.76)  LDD1250  [3]
 LDCM0372  CL103 PaTu 8988t C316(0.79)  LDD1251  [3]
 LDCM0373  CL104 PaTu 8988t C316(0.75)  LDD1252  [3]
 LDCM0374  CL105 HCT 116 C316(0.81)  LDD0691  [3]
 LDCM0375  CL106 HCT 116 C316(0.81)  LDD0692  [3]
 LDCM0376  CL107 HCT 116 C316(0.80)  LDD0693  [3]
 LDCM0377  CL108 HCT 116 C316(0.95)  LDD0694  [3]
 LDCM0378  CL109 HCT 116 C316(0.82)  LDD0695  [3]
 LDCM0379  CL11 HCT 116 C316(1.02)  LDD0696  [3]
 LDCM0380  CL110 HCT 116 C316(0.77)  LDD0697  [3]
 LDCM0381  CL111 HCT 116 C316(0.80)  LDD0698  [3]
 LDCM0382  CL112 HCT 116 C316(0.94)  LDD0699  [3]
 LDCM0383  CL113 HCT 116 C316(0.68)  LDD0700  [3]
 LDCM0384  CL114 HCT 116 C316(0.88)  LDD0701  [3]
 LDCM0385  CL115 HCT 116 C316(0.72)  LDD0702  [3]
 LDCM0386  CL116 HCT 116 C316(0.81)  LDD0703  [3]
 LDCM0387  CL117 HCT 116 C316(1.25)  LDD0704  [3]
 LDCM0388  CL118 HCT 116 C316(1.07)  LDD0705  [3]
 LDCM0389  CL119 HCT 116 C316(1.00)  LDD0706  [3]
 LDCM0390  CL12 HCT 116 C316(1.00)  LDD0707  [3]
 LDCM0391  CL120 HCT 116 C316(1.11)  LDD0708  [3]
 LDCM0392  CL121 HCT 116 C316(0.93)  LDD0709  [3]
 LDCM0393  CL122 HCT 116 C316(1.10)  LDD0710  [3]
 LDCM0394  CL123 HCT 116 C316(0.87)  LDD0711  [3]
 LDCM0395  CL124 HCT 116 C316(1.01)  LDD0712  [3]
 LDCM0396  CL125 HCT 116 C316(1.06)  LDD0713  [3]
 LDCM0397  CL126 HCT 116 C316(0.85)  LDD0714  [3]
 LDCM0398  CL127 HCT 116 C316(0.91)  LDD0715  [3]
 LDCM0399  CL128 HCT 116 C316(1.05)  LDD0716  [3]
 LDCM0400  CL13 HCT 116 C316(0.98)  LDD0717  [3]
 LDCM0401  CL14 HCT 116 C316(0.96)  LDD0718  [3]
 LDCM0402  CL15 HCT 116 C316(1.09)  LDD0719  [3]
 LDCM0403  CL16 HCT 116 C316(0.91)  LDD0720  [3]
 LDCM0404  CL17 HCT 116 C316(0.93)  LDD0721  [3]
 LDCM0405  CL18 HCT 116 C316(0.85)  LDD0722  [3]
 LDCM0406  CL19 HCT 116 C316(0.86)  LDD0723  [3]
 LDCM0407  CL2 HCT 116 C316(1.19)  LDD0724  [3]
 LDCM0408  CL20 HCT 116 C316(0.74)  LDD0725  [3]
 LDCM0409  CL21 HCT 116 C316(0.75)  LDD0726  [3]
 LDCM0410  CL22 HCT 116 C316(0.87)  LDD0727  [3]
 LDCM0411  CL23 HCT 116 C316(0.74)  LDD0728  [3]
 LDCM0412  CL24 HCT 116 C316(0.83)  LDD0729  [3]
 LDCM0413  CL25 HCT 116 C316(0.83)  LDD0730  [3]
 LDCM0414  CL26 HCT 116 C316(0.92)  LDD0731  [3]
 LDCM0415  CL27 HCT 116 C316(0.76)  LDD0732  [3]
 LDCM0416  CL28 HCT 116 C316(0.74)  LDD0733  [3]
 LDCM0417  CL29 HCT 116 C316(0.87)  LDD0734  [3]
 LDCM0418  CL3 HCT 116 C316(1.19)  LDD0735  [3]
 LDCM0419  CL30 HCT 116 C316(1.02)  LDD0736  [3]
 LDCM0420  CL31 HCT 116 C316(1.08)  LDD0737  [3]
 LDCM0421  CL32 HCT 116 C316(1.09)  LDD0738  [3]
 LDCM0422  CL33 HCT 116 C316(1.01)  LDD0739  [3]
 LDCM0423  CL34 HCT 116 C316(1.08)  LDD0740  [3]
 LDCM0424  CL35 HCT 116 C316(0.89)  LDD0741  [3]
 LDCM0425  CL36 HCT 116 C316(1.11)  LDD0742  [3]
 LDCM0426  CL37 HCT 116 C316(1.00)  LDD0743  [3]
 LDCM0428  CL39 HCT 116 C316(1.11)  LDD0745  [3]
 LDCM0429  CL4 HCT 116 C316(0.93)  LDD0746  [3]
 LDCM0430  CL40 HCT 116 C316(1.21)  LDD0747  [3]
 LDCM0431  CL41 HCT 116 C316(1.10)  LDD0748  [3]
 LDCM0432  CL42 HCT 116 C316(1.02)  LDD0749  [3]
 LDCM0433  CL43 HCT 116 C316(1.14)  LDD0750  [3]
 LDCM0434  CL44 HCT 116 C316(1.04)  LDD0751  [3]
 LDCM0435  CL45 HCT 116 C316(1.15)  LDD0752  [3]
 LDCM0436  CL46 HCT 116 C316(0.90)  LDD0753  [3]
 LDCM0437  CL47 HCT 116 C316(1.04)  LDD0754  [3]
 LDCM0438  CL48 HCT 116 C316(0.87)  LDD0755  [3]
 LDCM0439  CL49 HCT 116 C316(1.12)  LDD0756  [3]
 LDCM0440  CL5 HCT 116 C316(0.98)  LDD0757  [3]
 LDCM0441  CL50 HCT 116 C316(0.90)  LDD0758  [3]
 LDCM0442  CL51 HCT 116 C316(1.18)  LDD0759  [3]
 LDCM0443  CL52 HCT 116 C316(0.80)  LDD0760  [3]
 LDCM0444  CL53 HCT 116 C316(0.79)  LDD0761  [3]
 LDCM0445  CL54 HCT 116 C316(0.87)  LDD0762  [3]
 LDCM0446  CL55 HCT 116 C316(1.00)  LDD0763  [3]
 LDCM0447  CL56 HCT 116 C316(1.13)  LDD0764  [3]
 LDCM0448  CL57 HCT 116 C316(0.96)  LDD0765  [3]
 LDCM0449  CL58 HCT 116 C316(1.03)  LDD0766  [3]
 LDCM0450  CL59 HCT 116 C316(1.09)  LDD0767  [3]
 LDCM0451  CL6 HCT 116 C316(1.06)  LDD0768  [3]
 LDCM0452  CL60 HCT 116 C316(1.09)  LDD0769  [3]
 LDCM0453  CL61 HCT 116 C316(0.91)  LDD0770  [3]
 LDCM0454  CL62 HCT 116 C316(0.92)  LDD0771  [3]
 LDCM0455  CL63 HCT 116 C316(0.75)  LDD0772  [3]
 LDCM0456  CL64 HCT 116 C316(0.81)  LDD0773  [3]
 LDCM0457  CL65 HCT 116 C316(0.91)  LDD0774  [3]
 LDCM0458  CL66 HCT 116 C316(0.75)  LDD0775  [3]
 LDCM0459  CL67 HCT 116 C316(0.74)  LDD0776  [3]
 LDCM0460  CL68 HCT 116 C316(0.70)  LDD0777  [3]
 LDCM0461  CL69 HCT 116 C316(0.72)  LDD0778  [3]
 LDCM0462  CL7 HCT 116 C316(1.05)  LDD0779  [3]
 LDCM0463  CL70 HCT 116 C316(0.79)  LDD0780  [3]
 LDCM0464  CL71 HCT 116 C316(0.70)  LDD0781  [3]
 LDCM0465  CL72 HCT 116 C316(0.77)  LDD0782  [3]
 LDCM0466  CL73 HCT 116 C316(0.71)  LDD0783  [3]
 LDCM0467  CL74 HCT 116 C316(0.70)  LDD0784  [3]
 LDCM0469  CL76 HCT 116 C316(0.91)  LDD0786  [3]
 LDCM0470  CL77 HCT 116 C316(0.85)  LDD0787  [3]
 LDCM0471  CL78 HCT 116 C316(0.93)  LDD0788  [3]
 LDCM0472  CL79 HCT 116 C316(0.89)  LDD0789  [3]
 LDCM0473  CL8 HCT 116 C316(0.88)  LDD0790  [3]
 LDCM0474  CL80 HCT 116 C316(1.07)  LDD0791  [3]
 LDCM0475  CL81 HCT 116 C316(0.91)  LDD0792  [3]
 LDCM0476  CL82 HCT 116 C316(1.10)  LDD0793  [3]
 LDCM0477  CL83 HCT 116 C316(0.90)  LDD0794  [3]
 LDCM0478  CL84 HCT 116 C316(0.98)  LDD0795  [3]
 LDCM0479  CL85 HCT 116 C316(0.86)  LDD0796  [3]
 LDCM0480  CL86 HCT 116 C316(0.83)  LDD0797  [3]
 LDCM0481  CL87 HCT 116 C316(0.82)  LDD0798  [3]
 LDCM0482  CL88 HCT 116 C316(0.81)  LDD0799  [3]
 LDCM0483  CL89 HCT 116 C316(0.94)  LDD0800  [3]
 LDCM0484  CL9 HCT 116 C316(0.92)  LDD0801  [3]
 LDCM0485  CL90 HCT 116 C316(1.01)  LDD0802  [3]
 LDCM0486  CL91 HCT 116 C316(0.90)  LDD0803  [3]
 LDCM0487  CL92 HCT 116 C316(1.09)  LDD0804  [3]
 LDCM0488  CL93 HCT 116 C316(1.14)  LDD0805  [3]
 LDCM0489  CL94 HCT 116 C316(0.91)  LDD0806  [3]
 LDCM0490  CL95 HCT 116 C316(0.91)  LDD0807  [3]
 LDCM0491  CL96 HCT 116 C316(0.95)  LDD0808  [3]
 LDCM0492  CL97 HCT 116 C316(0.91)  LDD0809  [3]
 LDCM0493  CL98 HCT 116 C316(0.91)  LDD0810  [3]
 LDCM0494  CL99 HCT 116 C316(0.90)  LDD0811  [3]
 LDCM0182  Compound 18 HEK-293T 10.15  LDD0501  [9]
 LDCM0191  Compound 21 HEK-293T 8.73  LDD0493  [9]
 LDCM0190  Compound 34 HEK-293T 4.91  LDD0497  [9]
 LDCM0192  Compound 35 HEK-293T 4.55  LDD0491  [9]
 LDCM0193  Compound 36 HEK-293T 9.80  LDD0494  [9]
 LDCM0195  Compound 38 HEK-293T 4.28  LDD0499  [9]
 LDCM0196  Compound 39 HEK-293T 8.70  LDD0496  [9]
 LDCM0197  Compound 40 HEK-293T 12.99  LDD0495  [9]
 LDCM0495  E2913 HEK-293T C316(0.85); C270(0.99)  LDD1698  [14]
 LDCM0625  F8 Ramos C316(1.09)  LDD2187  [15]
 LDCM0082  FK866 A-549 3.29  LDD0142  [12]
 LDCM0573  Fragment11 Ramos C316(2.21)  LDD2190  [15]
 LDCM0574  Fragment12 Ramos C316(8.46)  LDD2191  [15]
 LDCM0575  Fragment13 Ramos C316(1.27)  LDD2192  [15]
 LDCM0582  Fragment23 Ramos C316(1.09)  LDD2196  [15]
 LDCM0586  Fragment28 Ramos C316(0.53)  LDD2198  [15]
 LDCM0588  Fragment30 Ramos C316(2.83)  LDD2199  [15]
 LDCM0468  Fragment33 HCT 116 C316(0.74)  LDD0785  [3]
 LDCM0427  Fragment51 HCT 116 C316(1.62)  LDD0744  [3]
 LDCM0022  KB02 HCT 116 C316(2.07)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C316(0.59)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C316(1.04)  LDD0082  [3]
 LDCM0084  Ro 48-8071 A-549 2.71  LDD0145  [12]

References

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2 Minimalist linkers suitable for irreversible inhibitors in simultaneous proteome profiling, live-cell imaging and drug screening. Chem Commun (Camb). 2019 Jan 15;55(6):834-837. doi: 10.1039/c8cc08685k.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
10 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
11 Cell- and Tissue-Based Proteome Profiling and Bioimaging with Probes Derived from a Potent AXL Kinase Inhibitor. Chem Asian J. 2018 Sep 17;13(18):2601-2605. doi: 10.1002/asia.201800605. Epub 2018 Aug 7.
12 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
13 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
14 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
15 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578