Probe Name	FFF probe6					Probe Info
Probe concentration	Probe:200uM; negative probe:200uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	SILAC
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP06612	2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial (DECR1)					20.0
LDTP18414	5'-nucleotidase domain-containing protein 2 (NT5DC2)					20.0
LDTP10982	Aconitate hydratase, mitochondrial (ACO2)					20.0
LDTP00150	Acyl-coenzyme A diphosphatase NUDT19 (NUDT19)					20.0
LDTP01687	Apoptosis-inducing factor 1, mitochondrial (AIFM1)					20.0
LDTP01773	Cytochrome c oxidase subunit 2 (MT-CO2)					20.0
LDTP13709	Dolichol kinase (DOLK)					20.0
LDTP13702	E3 ubiquitin-protein ligase TRIM33 (TRIM33)					20.0
LDTP02175	Epoxide hydrolase 1 (EPHX1)					20.0
LDTP12253	Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)					20.0
LDTP03540	Heme oxygenase 2 (HMOX2)					20.0
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					20.0
LDTP06796	Mitochondrial 10-formyltetrahydrofolate dehydrogenase (ALDH1L2)					20.0
LDTP08596	Mitochondrial Rho GTPase 2 (RHOT2)					20.0
LDTP05862	NAD(P) transhydrogenase, mitochondrial (NNT)					20.0
LDTP11081	NAD-capped RNA hydrolase NUDT12 (NUDT12)					20.0
LDTP04051	Nicotinamide phosphoribosyltransferase (NAMPT)					20.0
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					20.0
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					19.1
LDTP06150	DNA replication licensing factor MCM6 (MCM6)					18.4
LDTP00890	S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1)					17.0
LDTP11215	Plasma alpha-L-fucosidase (FUCA2)					16.4
LDTP03419	Proteasome subunit beta type-5 (PSMB5)					15.3
LDTP10188	FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1)					15.3
LDTP06750	Prolyl 3-hydroxylase 1 (P3H1)					15.2
LDTP01768	Glutamate dehydrogenase 1, mitochondrial (GLUD1)					15.1
LDTP06720	Peroxisomal leader peptide-processing protease (TYSND1)					14.8
LDTP04471	Ribosomal protein S6 kinase alpha-3 (RPS6KA3)					14.2
LDTP06384	Ribosomal protein S6 kinase alpha-1 (RPS6KA1)					13.9
LDTP06307	Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1)					13.8
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					13.0
LDTP00246	Eukaryotic translation initiation factor 3 subunit F (EIF3F)					12.8
LDTP12279	Endoplasmic reticulum transmembrane helix translocase (ATP13A1)					12.7
LDTP13751	Exonuclease 1 (EXO1)					12.6
LDTP06849	Prolyl endopeptidase-like (PREPL)					12.5
LDTP04284	Proteasome subunit beta type-3 (PSMB3)					12.4
LDTP11841	ATP-dependent DNA/RNA helicase DHX36 (DHX36)					12.4
LDTP07504	Lysophospholipid acyltransferase 5 (LPCAT3)					12.3
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					11.2
LDTP07154	ATPase family AAA domain-containing protein 3B (ATAD3B)					11.0
LDTP04374	Dynamin-2 (DNM2)					10.8
LDTP00886	Nardilysin (NRDC)					9.7
LDTP05447	Dynamin-1 (DNM1)					9.7
LDTP13744	Dynamin-3 (DNM3)					9.5
LDTP00267	DNA-directed RNA polymerase, mitochondrial (POLRMT)					9.4
LDTP04032	Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2)					9.3
LDTP04251	Elongation factor Tu, mitochondrial (TUFM)					9.1
LDTP00601	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT)					9.0
LDTP02150	ATP synthase subunit beta, mitochondrial (ATP5F1B)					8.8
LDTP19391	ATPase family AAA domain-containing protein 3C (ATAD3C)					8.7
LDTP05724	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)					8.7
LDTP12588	Isoleucine--tRNA ligase, mitochondrial (IARS2)					8.5
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					8.4
LDTP03189	Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2)					7.8
LDTP03330	Proteasome subunit alpha type-1 (PSMA1)					7.7
LDTP01328	CAAX prenyl protease 1 homolog (ZMPSTE24)					7.5
LDTP12048	Complex I assembly factor ACAD9, mitochondrial (ACAD9)					7.4
LDTP07003	Presequence protease, mitochondrial (PITRM1)					7.3
LDTP01245	Enoyl-CoA delta isomerase 2 (ECI2)					7.2
LDTP03680	DNA replication licensing factor MCM4 (MCM4)					7.1
LDTP04634	Alpha-N-acetylglucosaminidase (NAGLU)					7.1
LDTP13344	Tyrosine-protein kinase BAZ1B (BAZ1B)					7.1
LDTP03817	Lon protease homolog, mitochondrial (LONP1)					6.9
LDTP03333	Proteasome subunit alpha type-4 (PSMA4)					6.9
LDTP02671	Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3)					6.8
LDTP06905	Acylglycerol kinase, mitochondrial (AGK)					6.6
LDTP03813	Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial (CPOX)					6.6
LDTP02375	Poly [ADP-ribose] polymerase 1 (PARP1)					6.6
LDTP04120	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A)					6.5
LDTP05632	Mitochondrial-processing peptidase subunit alpha (PMPCA)					6.5
LDTP12681	ATPase family AAA domain-containing protein 3A (ATAD3A)					6.3
LDTP02198	Cathepsin D (CTSD)					6.1
LDTP09110	NAD(P)H-hydrate epimerase (NAXE)					6.1
LDTP00464	Glutathione S-transferase 3, mitochondrial (MGST3)					6.0
LDTP00988	Protein O-GlcNAcase (OGA)					5.9
LDTP04211	Isocitrate dehydrogenase [NADP], mitochondrial (IDH2)					5.8
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					5.7
LDTP02534	Endoplasmic reticulum chaperone BiP (HSPA5)					5.6
LDTP11319	Threonine--tRNA ligase, mitochondrial (TARS2)					5.6
LDTP11819	STE20-like serine/threonine-protein kinase (SLK)					5.4
LDTP04519	Kinesin-like protein KIF11 (KIF11)					5.3
LDTP03765	Myosin-10 (MYH10)					5.3
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					5.2
LDTP07651	Monofunctional C1-tetrahydrofolate synthase, mitochondrial (MTHFD1L)					5.1

Target ID	Target Name					Binding Ratio
LDTP11116	45 kDa calcium-binding protein (SDF4)					20.0
LDTP10036	BOS complex subunit NCLN (NCLN)					20.0
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					20.0
LDTP05900	Metaxin-1 (MTX1)					20.0
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					20.0
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					20.0
LDTP14952	Mpv17-like protein 2 (MPV17L2)					20.0
LDTP11213	Nucleoporin NDC1 (NDC1)					20.0
LDTP04932	Protein transport protein Sec61 subunit alpha isoform 1 (SEC61A1)					20.0
LDTP01745	Putative lipid scramblase CLPTM1 (CLPTM1)					20.0
LDTP14084	Sorting and assembly machinery component 50 homolog (SAMM50)					20.0
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					20.0
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					18.9
LDTP06426	Translocating chain-associated membrane protein 1 (TRAM1)					18.8
LDTP06660	MICOS complex subunit MIC60 (IMMT)					17.8
LDTP01525	Mitochondrial proton/calcium exchanger protein (LETM1)					16.3
LDTP13393	Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial (SLC25A13)					13.4
LDTP05238	Solute carrier family 25 member 3 (SLC25A3)					12.9
LDTP00562	Membrane-associated progesterone receptor component 2 (PGRMC2)					11.3
LDTP02215	Prosaposin (PSAP)					10.7
LDTP03405	Calnexin (CANX)					10.1
LDTP07156	Protein wntless homolog (WLS)					9.8
LDTP01043	Sorting nexin-2 (SNX2)					9.4
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					8.8
LDTP12042	Proton-activated chloride channel (PACC1)					8.7
LDTP14235	Mitochondrial carrier homolog 2 (MTCH2)					8.3
LDTP13243	Translocation protein SEC63 homolog (SEC63)					7.9
LDTP00592	Surfeit locus protein 4 (SURF4)					7.5
LDTP12090	Sideroflexin-1 (SFXN1)					7.2
LDTP02057	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1)					7.2
LDTP08769	Nuclear pore complex protein Nup93 (NUP93)					6.8
LDTP09968	V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1)					6.6
LDTP10059	PAT complex subunit CCDC47 (CCDC47)					6.2
LDTP13416	Stomatin-like protein 2, mitochondrial (STOML2)					6.2
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					6.1
LDTP00821	Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44)					6.0
LDTP03402	Calreticulin (CALR)					5.8
LDTP11531	Oxysterol-binding protein-related protein 8 (OSBPL8)					5.5
LDTP03810	ATP synthase subunit gamma, mitochondrial (ATP5F1C)					5.3
LDTP02058	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (RPN2)					5.3
LDTP13824	Voltage-dependent anion-selective channel protein 3 (VDAC3)					5.1
LDTP04707	Protein SEC13 homolog (SEC13)					5.1
LDTP02612	Nucleoprotein TPR (TPR)					5.0

Target ID	Target Name					Binding Ratio
LDTP09694	Paraspeckle component 1 (PSPC1)					13.9
LDTP05065	Y-box-binding protein 1 (YBX1)					9.4
LDTP10891	DnaJ homolog subfamily C member 2 (DNAJC2)					8.5
LDTP08216	Protein polybromo-1 (PBRM1)					6.6
LDTP09932	SWI/SNF complex subunit SMARCC1 (SMARCC1)					5.7
LDTP06341	Non-POU domain-containing octamer-binding protein (NONO)					5.6
LDTP10868	Cell division cycle 5-like protein (CDC5L)					5.3

Target ID	Target Name					Binding Ratio
LDTP00887	Calumenin (CALU)					20.0
LDTP12677	DnaJ homolog subfamily C member 11 (DNAJC11)					20.0
LDTP05400	Lamin-B2 (LMNB2)					20.0
LDTP15269	Large ribosomal subunit protein mL55 (MRPL55)					20.0
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					20.0
LDTP05375	Nucleobindin-1 (NUCB1)					20.0
LDTP05160	Nucleobindin-2 (NUCB2)					20.0
LDTP01208	Pre-mRNA-processing factor 40 homolog A (PRPF40A)					20.0
LDTP01608	Proteasome assembly chaperone 1 (PSMG1)					20.0
LDTP16080	Protein FAM114A2 (FAM114A2)					20.0
LDTP11046	RNA-binding protein 4B (RBM4B)					20.0
LDTP06181	Structural maintenance of chromosomes protein 1A (SMC1A)					20.0
LDTP07368	Tetratricopeptide repeat protein 19, mitochondrial (TTC19)					20.0
LDTP10295	Zinc finger protein 503 (ZNF503)					20.0
LDTP01932	Prelamin-A/C (LMNA)					17.4
LDTP06097	Reticulocalbin-2 (RCN2)					15.8
LDTP02297	Vimentin (VIM)					15.0
LDTP13630	Neudesin (NENF)					14.3
LDTP04652	Eukaryotic translation initiation factor 5 (EIF5)					13.3
LDTP10684	RNA-binding protein 14 (RBM14)					13.3
LDTP09878	Symplekin (SYMPK)					13.0
LDTP04396	RNA-binding protein FXR2 (FXR2)					12.8
LDTP04679	Afadin (AFDN)					12.5
LDTP06881	Programmed cell death protein 4 (PDCD4)					12.5
LDTP04708	NHP2-like protein 1 (SNU13)					12.2
LDTP04094	Large proline-rich protein BAG6 (BAG6)					12.2
LDTP10185	FAS-associated factor 2 (FAF2)					12.1
LDTP03404	Microtubule-associated protein 4 (MAP4)					12.0
LDTP14486	Clustered mitochondria protein homolog (CLUH)					11.6
LDTP11239	Protein misato homolog 1 (MSTO1)					10.7
LDTP11277	Tubulin beta-2B chain (TUBB2B)					9.9
LDTP02205	Tubulin beta chain (TUBB)					9.8
LDTP08130	Rab9 effector protein with kelch motifs (RABEPK)					9.7
LDTP08238	Kinectin (KTN1)					9.5
LDTP05076	Tubulin beta-4B chain (TUBB4B)					9.0
LDTP06809	Tubulin beta-8 chain (TUBB8)					8.8
LDTP05904	Tubulin beta-3 chain (TUBB3)					8.8
LDTP10924	Prohibitin-2 (PHB2)					8.3
LDTP11233	Tubulin beta-6 chain (TUBB6)					8.1
LDTP15705	BTB/POZ domain-containing protein KCTD12 (KCTD12)					7.9
LDTP08594	Negative elongation factor C/D (NELFCD)					7.6
LDTP03065	Lamin-B1 (LMNB1)					7.5
LDTP03614	Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3)					7.2
LDTP03775	RNA-binding protein FUS (FUS)					7.2
LDTP13338	Vacuolar protein sorting-associated protein 51 homolog (VPS51)					7.2
LDTP13519	Proteasome activator complex subunit 2 (PSME2)					7.0
LDTP06393	Splicing factor 3A subunit 1 (SF3A1)					7.0
LDTP17640	RNA-binding protein 12B (RBM12B)					7.0
LDTP04201	T-complex protein 1 subunit epsilon (CCT5)					6.4
LDTP03322	Bromodomain-containing protein 2 (BRD2)					6.1
LDTP05922	Dynactin subunit 2 (DCTN2)					6.1
LDTP09938	Far upstream element-binding protein 2 (KHSRP)					6.1
LDTP02734	Endoplasmin (HSP90B1)					6.0
LDTP10078	Far upstream element-binding protein 1 (FUBP1)					5.8
LDTP00756	Heterogeneous nuclear ribonucleoprotein R (HNRNPR)					5.8
LDTP18146	RNA binding motif protein, X-linked-like-1 (RBMXL1)					5.7
LDTP13591	A-kinase anchor protein 8-like (AKAP8L)					5.6
LDTP00991	Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP)					5.5
LDTP04290	YLP motif-containing protein 1 (YLPM1)					5.5
LDTP04500	Heterogeneous nuclear ribonucleoprotein M (HNRNPM)					5.4
LDTP04356	Emerin (EMD)					5.3
LDTP09793	Small ribosomal subunit protein mS27 (MRPS27)					5.3
LDTP03850	RNA-binding motif protein, X chromosome (RBMX)					5.2
LDTP03615	Heterogeneous nuclear ribonucleoprotein H (HNRNPH1)					5.2
LDTP10285	Small ribosomal subunit protein mS39 (PTCD3)					5.2
LDTP17065	Heterogeneous nuclear ribonucleoprotein U-like protein 2 (HNRNPUL2)					5.1
LDTP00862	Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA)					5.1
LDTP01432	Mitochondrial import receptor subunit TOM70 (TOMM70)					5.0
LDTP19912	Protein NipSnap homolog 1 (NIPSNAP1)					5.0

1	Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.