Details of the Target

General Information of Target

Target ID LDTP06809
Target Name Tubulin beta-8 chain (TUBB8)
Gene Name TUBB8
Gene ID 347688
Synonyms
Tubulin beta-8 chain; Tubulin beta 8 class VIII
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MREIVLTQIGQCGNQIGAKFWEVISDEHAIDSAGTYHGDSHLQLERINVYYNEASGGRYV
PRAVLVDLEPGTMDSVRSGPFGQVFRPDNFIFGQCGAGNNWAKGHYTEGAELMESVMDVV
RKEAESCDCLQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRIINTFSILPSPKVSDTVV
EPYNATLSVHQLIENADETFCIDNEALYDICSKTLKLPTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVAELTQQMFDAKNMM
AACDPRHGRYLTAAAIFRGRMPMREVDEQMFNIQDKNSSYFADWLPNNVKTAVCDIPPRG
LKMSATFIGNNTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATAEEEEDEEYAEEEVA
Target Bioclass
Other
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB8 has a key role in meiotic spindle assembly and oocyte maturation.
Uniprot ID
Q3ZCM7
Ensemble ID
ENST00000568584.6
HGNC ID
HGNC:20773
ChEMBL ID
CHEMBL2095182

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
AZ-5
 Probe Info  		2.43  LDD0394  [2]
N1
 Probe Info  		100.00  LDD0242  [3]
DBIA
 Probe Info  		C406(1.32)  LDD3314  [4]
P26
 Probe Info  		1.81  LDD0409  [5]
BTD
 Probe Info  		C12(1.71)  LDD2096  [6]
NAIA_5
 Probe Info  		C127(0.09)  LDD2227  [7]
AHL-Pu-1
 Probe Info  		C129(2.47); C303(2.15)  LDD0168  [8]
Alkyne-RA190
 Probe Info  		2.10  LDD0299  [9]
HHS-475
 Probe Info  		Y281(0.79)  LDD0264  [10]
HHS-465
 Probe Info  		Y159(8.69); Y222(6.05); Y281(7.61)  LDD2237  [11]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [12]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [12]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [13]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [12]
ATP probe
 Probe Info  		K362(0.00); K252(0.00)  LDD0035  [14]
WYneO
 Probe Info  		C303(0.00); C354(0.00); C12(0.00)  LDD0022  [15]
ENE
 Probe Info  		C12(0.00); C303(0.00); C354(0.00)  LDD0006  [15]
IPM
 Probe Info  		C12(0.00); C303(0.00); C239(0.00); C354(0.00)  LDD0005  [15]
TFBX
 Probe Info  		C303(0.00); C95(0.00); C354(0.00)  LDD0148  [16]
VSF
 Probe Info  		N.A.  LDD0007  [15]
1c-yne
 Probe Info  		N.A.  LDD0228  [17]
Acrolein
 Probe Info  		C354(0.00); C303(0.00); C129(0.00); C127(0.00)  LDD0217  [18]
Cinnamaldehyde
 Probe Info  		C354(0.00); C303(0.00)  LDD0220  [18]
Crotonaldehyde
 Probe Info  		C354(0.00); C303(0.00)  LDD0219  [18]
Methacrolein
 Probe Info  		C127(0.00); C354(0.00); C303(0.00)  LDD0218  [18]
AOyne
 Probe Info  		15.00  LDD0443  [19]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0472  [20]
FFF probe3
 Probe Info  		8.61  LDD0464  [20]
FFF probe6
 Probe Info  		8.79  LDD0467  [20]
STS-2
 Probe Info  		N.A.  LDD0138  [21]
DA-2
 Probe Info  		N.A.  LDD0070  [22]
STS-1
 Probe Info  		N.A.  LDD0069  [23]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C129(2.47); C303(2.15)  LDD0168  [8]
 LDCM0108  Chloroacetamide HeLa H264(0.00); C354(0.00); C303(0.00); C129(0.00)  LDD0222  [18]
 LDCM0632  CL-Sc Hep-G2 C127(0.09)  LDD2227  [7]
 LDCM0182  Compound 18 HEK-293T 9.95  LDD0501  [20]
 LDCM0191  Compound 21 HEK-293T 4.44  LDD0508  [20]
 LDCM0181  Compound 41 HEK-293T 8.85  LDD0502  [20]
 LDCM0183  Compound 42 HEK-293T 5.15  LDD0500  [20]
 LDCM0116  HHS-0101 DM93 Y281(0.79)  LDD0264  [10]
 LDCM0117  HHS-0201 DM93 Y281(0.80)  LDD0265  [10]
 LDCM0118  HHS-0301 DM93 Y281(0.85)  LDD0266  [10]
 LDCM0119  HHS-0401 DM93 Y281(0.91)  LDD0267  [10]
 LDCM0120  HHS-0701 DM93 Y281(0.76)  LDD0268  [10]
 LDCM0107  IAA HeLa C354(0.00); C303(0.00); H137(0.00)  LDD0221  [18]
 LDCM0022  KB02 A-375 C406(1.70)  LDD2255  [4]
 LDCM0023  KB03 A-375 C406(2.62)  LDD2672  [4]
 LDCM0024  KB05 IGR37 C406(1.32)  LDD3314  [4]
 LDCM0109  NEM HeLa H264(0.00); H137(0.00)  LDD0223  [18]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C12(1.71)  LDD2096  [6]
 LDCM0159  P28 BxPC-3 1.81  LDD0409  [5]
 LDCM0131  RA190 MM1.R 2.10  LDD0299  [9]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Integrated phenotypic screening and activity-based protein profiling to reveal potential therapy targets of pancreatic cancer. Chem Commun (Camb). 2019 Jan 31;55(11):1596-1599. doi: 10.1039/c8cc08753a.
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
9 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
10 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
11 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
12 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
13 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
14 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
15 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
16 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
17 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
18 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
19 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
20 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
23 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.