Details of the Target

General Information of Target

Target ID LDTP13709
Target Name Dolichol kinase (DOLK)
Gene Name DOLK
Gene ID 22845
Synonyms
KIAA1094; TMEM15; Dolichol kinase; EC 2.7.1.108; Transmembrane protein 15
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGFELDRFDGDVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQEGSCPARCRGRL
SAKELNHVLPLKRLILKLDIKCAYATRGCGRVVKLQQLPEHLERCDFAPARCRHAGCGQV
LLRRDVEAHMRDACDARPVGRCQEGCGLPLTHGEQRAGGHCCARALRAHNGALQARLGAL
HKALKKEALRAGKREKSLVAQLAAAQLELQMTALRYQKKFTEYSARLDSLSRCVAAPPGG
KGEETKSLTLVLHRDSGSLGFNIIGGRPSVDNHDGSSSEGIFVSKIVDSGPAAKEGGLQI
HDRIIEVNGRDLSRATHDQAVEAFKTAKEPIVVQVLRRTPRTKMFTPPSESQLVDTGTQT
DITFEHIMALTKMSSPSPPVLDPYLLPEEHPSAHEYYDPNDYIGDIHQEMDREELELEEV
DLYRMNSQDKLGLTVCYRTDDEDDIGIYISEIDPNSIAAKDGRIREGDRIIQINGIEVQN
REEAVALLTSEENKNFSLLIARPELQLDEGWMDDDRNDFLDDLHMDMLEEQHHQAMQFTA
SVLQQKKHDEDGGTTDTATILSNQHEKDSGVGRTDESTRNDESSEQENNGDDATASSNPL
AGQRKLTCSQDTLGSGDLPFSNESFISADCTDADYLGIPVDECERFRELLELKCQVKSAT
PYGLYYPSGPLDAGKSDPESVDKELELLNEELRSIELECLSIVRAHKMQQLKEQYRESWM
LHNSGFRNYNTSIDVRRHELSDITELPEKSDKDSSSAYNTGESCRSTPLTLEISPDNSLR
RAAEGISCPSSEGAVGTTEAYGPASKNLLSITEDPEVGTPTYSPSLKELDPNQPLESKER
RASDGSRSPTPSQKLGSAYLPSYHHSPYKHAHIPAHAQHYQSYMQLIQQKSAVEYAQSQM
SLVSMCKDLSSPTPSEPRMEWKVKIRSDGTRYITKRPVRDRLLRERALKIREERSGMTTD
DDAVSEMKMGRYWSKEERKQHLVKAKEQRRRREFMMQSRLDCLKEQQAADDRKEMNILEL
SHKKMMKKRNKKIFDNWMTIQELLTHGTKSPDGTRVYNSFLSVTTV
Target Bioclass
Enzyme
Family
Polyprenol kinase family
Subcellular location
Endoplasmic reticulum membrane
Function
Catalyzes CTP-mediated phosphorylation of dolichol, the terminal step in de novo dolichyl monophosphate (Dol-P) biosynthesis. Dol-P is a lipid carrier essential for the synthesis of N-linked and O-linked oligosaccharides and for GPI anchors.
Uniprot ID
Q9UPQ8
Ensemble ID
ENST00000372586.4
HGNC ID
HGNC:23406

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C99(1.06)  LDD1507  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0471  [3]
FFF probe6
 Probe Info  		20.00  LDD0467  [3]
OEA-DA
 Probe Info  		17.52  LDD0046  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C99(1.06)  LDD1507  [1]
 LDCM0226  AC11 HEK-293T C99(1.02)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C99(1.10)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C99(1.02)  LDD1512  [1]
 LDCM0270  AC15 HEK-293T C99(1.11)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C99(1.08)  LDD1515  [1]
 LDCM0278  AC19 HEK-293T C99(1.00)  LDD1517  [1]
 LDCM0280  AC20 HEK-293T C99(1.03)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C99(0.98)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C99(0.89)  LDD1521  [1]
 LDCM0283  AC23 HEK-293T C99(0.99)  LDD1522  [1]
 LDCM0284  AC24 HEK-293T C99(0.99)  LDD1523  [1]
 LDCM0285  AC25 HEK-293T C99(1.06)  LDD1524  [1]
 LDCM0287  AC27 HEK-293T C99(0.93)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C99(1.16)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C99(1.04)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C99(0.89)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C99(0.90)  LDD1530  [1]
 LDCM0292  AC31 HEK-293T C99(0.99)  LDD1531  [1]
 LDCM0293  AC32 HEK-293T C99(0.98)  LDD1532  [1]
 LDCM0294  AC33 HEK-293T C99(1.08)  LDD1533  [1]
 LDCM0296  AC35 HEK-293T C99(0.96)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C99(0.93)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C99(0.95)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C99(1.03)  LDD1538  [1]
 LDCM0300  AC39 HEK-293T C99(1.11)  LDD1539  [1]
 LDCM0301  AC4 HEK-293T C99(0.83)  LDD1540  [1]
 LDCM0302  AC40 HEK-293T C99(1.07)  LDD1541  [1]
 LDCM0303  AC41 HEK-293T C99(1.08)  LDD1542  [1]
 LDCM0305  AC43 HEK-293T C99(0.93)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C99(0.93)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C99(0.99)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C99(0.99)  LDD1547  [1]
 LDCM0309  AC47 HEK-293T C99(0.95)  LDD1548  [1]
 LDCM0310  AC48 HEK-293T C99(1.00)  LDD1549  [1]
 LDCM0311  AC49 HEK-293T C99(1.08)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C99(0.93)  LDD1551  [1]
 LDCM0314  AC51 HEK-293T C99(0.95)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C99(1.17)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C99(0.97)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C99(1.00)  LDD1556  [1]
 LDCM0318  AC55 HEK-293T C99(1.03)  LDD1557  [1]
 LDCM0319  AC56 HEK-293T C99(1.05)  LDD1558  [1]
 LDCM0320  AC57 HEK-293T C99(1.13)  LDD1559  [1]
 LDCM0322  AC59 HEK-293T C99(0.92)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C99(0.96)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C99(1.00)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C99(1.06)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C99(1.00)  LDD1565  [1]
 LDCM0327  AC63 HEK-293T C99(1.19)  LDD1566  [1]
 LDCM0328  AC64 HEK-293T C99(1.04)  LDD1567  [1]
 LDCM0334  AC7 HEK-293T C99(0.94)  LDD1568  [1]
 LDCM0345  AC8 HEK-293T C99(0.90)  LDD1569  [1]
 LDCM0248  AKOS034007472 HEK-293T C99(1.09)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C99(1.04)  LDD1570  [1]
 LDCM0275  AKOS034007705 HEK-293T C99(1.03)  LDD1514  [1]
 LDCM0367  CL1 HEK-293T C99(1.05)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C99(1.27)  LDD1572  [1]
 LDCM0369  CL100 HEK-293T C99(1.01)  LDD1573  [1]
 LDCM0370  CL101 HEK-293T C99(1.02)  LDD1574  [1]
 LDCM0372  CL103 HEK-293T C99(1.26)  LDD1576  [1]
 LDCM0373  CL104 HEK-293T C99(1.03)  LDD1577  [1]
 LDCM0374  CL105 HEK-293T C99(1.17)  LDD1578  [1]
 LDCM0376  CL107 HEK-293T C99(1.02)  LDD1580  [1]
 LDCM0377  CL108 HEK-293T C99(1.05)  LDD1581  [1]
 LDCM0378  CL109 HEK-293T C99(1.06)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C99(1.20)  LDD1583  [1]
 LDCM0381  CL111 HEK-293T C99(1.18)  LDD1585  [1]
 LDCM0382  CL112 HEK-293T C99(1.08)  LDD1586  [1]
 LDCM0383  CL113 HEK-293T C99(0.94)  LDD1587  [1]
 LDCM0385  CL115 HEK-293T C99(1.19)  LDD1589  [1]
 LDCM0386  CL116 HEK-293T C99(0.99)  LDD1590  [1]
 LDCM0387  CL117 HEK-293T C99(1.19)  LDD1591  [1]
 LDCM0389  CL119 HEK-293T C99(1.21)  LDD1593  [1]
 LDCM0390  CL12 HEK-293T C99(1.20)  LDD1594  [1]
 LDCM0391  CL120 HEK-293T C99(1.05)  LDD1595  [1]
 LDCM0392  CL121 HEK-293T C99(1.10)  LDD1596  [1]
 LDCM0394  CL123 HEK-293T C99(1.43)  LDD1598  [1]
 LDCM0395  CL124 HEK-293T C99(1.04)  LDD1599  [1]
 LDCM0396  CL125 HEK-293T C99(1.08)  LDD1600  [1]
 LDCM0398  CL127 HEK-293T C99(1.36)  LDD1602  [1]
 LDCM0399  CL128 HEK-293T C99(1.09)  LDD1603  [1]
 LDCM0400  CL13 HEK-293T C99(1.33)  LDD1604  [1]
 LDCM0402  CL15 HEK-293T C99(1.30)  LDD1606  [1]
 LDCM0403  CL16 HEK-293T C99(1.05)  LDD1607  [1]
 LDCM0404  CL17 HEK-293T C99(1.19)  LDD1608  [1]
 LDCM0406  CL19 HEK-293T C99(1.11)  LDD1610  [1]
 LDCM0408  CL20 HEK-293T C99(1.19)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C99(1.30)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C99(1.22)  LDD1614  [1]
 LDCM0411  CL23 HEK-293T C99(1.43)  LDD1615  [1]
 LDCM0412  CL24 HEK-293T C99(1.21)  LDD1616  [1]
 LDCM0413  CL25 HEK-293T C99(1.13)  LDD1617  [1]
 LDCM0415  CL27 HEK-293T C99(1.17)  LDD1619  [1]
 LDCM0416  CL28 HEK-293T C99(1.14)  LDD1620  [1]
 LDCM0417  CL29 HEK-293T C99(1.03)  LDD1621  [1]
 LDCM0418  CL3 HEK-293T C99(1.26)  LDD1622  [1]
 LDCM0420  CL31 HEK-293T C99(0.98)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C99(1.05)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C99(1.66)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C99(1.02)  LDD1627  [1]
 LDCM0424  CL35 HEK-293T C99(1.39)  LDD1628  [1]
 LDCM0425  CL36 HEK-293T C99(1.18)  LDD1629  [1]
 LDCM0426  CL37 HEK-293T C99(1.09)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C99(1.37)  LDD1632  [1]
 LDCM0429  CL4 HEK-293T C99(1.13)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C99(0.94)  LDD1634  [1]
 LDCM0431  CL41 HEK-293T C99(1.05)  LDD1635  [1]
 LDCM0433  CL43 HEK-293T C99(1.04)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C99(1.09)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C99(1.12)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C99(1.09)  LDD1640  [1]
 LDCM0437  CL47 HEK-293T C99(1.63)  LDD1641  [1]
 LDCM0438  CL48 HEK-293T C99(1.15)  LDD1642  [1]
 LDCM0439  CL49 HEK-293T C99(1.35)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C99(1.15)  LDD1644  [1]
 LDCM0443  CL52 HEK-293T C99(1.04)  LDD1646  [1]
 LDCM0444  CL53 HEK-293T C99(1.19)  LDD1647  [1]
 LDCM0446  CL55 HEK-293T C99(1.02)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C99(1.23)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C99(1.25)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C99(1.26)  LDD1652  [1]
 LDCM0450  CL59 HEK-293T C99(1.32)  LDD1653  [1]
 LDCM0452  CL60 HEK-293T C99(1.20)  LDD1655  [1]
 LDCM0453  CL61 HEK-293T C99(1.02)  LDD1656  [1]
 LDCM0455  CL63 HEK-293T C99(1.03)  LDD1658  [1]
 LDCM0456  CL64 HEK-293T C99(1.07)  LDD1659  [1]
 LDCM0457  CL65 HEK-293T C99(1.12)  LDD1660  [1]
 LDCM0459  CL67 HEK-293T C99(0.97)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C99(1.21)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C99(1.14)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C99(1.15)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C99(1.09)  LDD1666  [1]
 LDCM0464  CL71 HEK-293T C99(1.21)  LDD1667  [1]
 LDCM0465  CL72 HEK-293T C99(1.22)  LDD1668  [1]
 LDCM0466  CL73 HEK-293T C99(1.15)  LDD1669  [1]
 LDCM0469  CL76 HEK-293T C99(1.02)  LDD1672  [1]
 LDCM0470  CL77 HEK-293T C99(1.10)  LDD1673  [1]
 LDCM0472  CL79 HEK-293T C99(0.97)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C99(2.07)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C99(0.88)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C99(1.08)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C99(1.11)  LDD1679  [1]
 LDCM0477  CL83 HEK-293T C99(1.41)  LDD1680  [1]
 LDCM0478  CL84 HEK-293T C99(1.34)  LDD1681  [1]
 LDCM0479  CL85 HEK-293T C99(1.34)  LDD1682  [1]
 LDCM0481  CL87 HEK-293T C99(1.25)  LDD1684  [1]
 LDCM0482  CL88 HEK-293T C99(1.09)  LDD1685  [1]
 LDCM0483  CL89 HEK-293T C99(1.12)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C99(1.20)  LDD1687  [1]
 LDCM0486  CL91 HEK-293T C99(1.07)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C99(1.10)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C99(1.18)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C99(1.10)  LDD1692  [1]
 LDCM0490  CL95 HEK-293T C99(1.43)  LDD1693  [1]
 LDCM0491  CL96 HEK-293T C99(1.23)  LDD1694  [1]
 LDCM0492  CL97 HEK-293T C99(1.05)  LDD1695  [1]
 LDCM0494  CL99 HEK-293T C99(1.02)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C99(1.10)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C99(1.26)  LDD1671  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable glutathione peroxidase 8 (GPX8) Glutathione peroxidase family Q8TED1
Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Y+L amino acid transporter 2 (SLC7A6) L-type amino acid transporter (LAT) (TC 2.A.3.8) family Q92536
Gap junction alpha-8 protein (GJA8) Connexin family P48165
Potassium voltage-gated channel subfamily A member 1 (KCNA1) Potassium channel family Q09470
Potassium voltage-gated channel subfamily A member 3 (KCNA3) Potassium channel family P22001
Potassium voltage-gated channel subfamily A member 6 (KCNA6) Potassium channel family P17658
Potassium voltage-gated channel subfamily A member 10 (KCNA10) Potassium channel family Q16322
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Immunoglobulin
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B-cell antigen receptor complex-associated protein alpha chain (CD79A) . P11912
Leucine-rich repeat-containing protein 4C (LRRC4C) . Q9HCJ2
V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR) . Q9H7M9
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ectodysplasin-A (EDA) Tumor necrosis factor family Q92838
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Synaptoporin (SYNPR) Synaptophysin/synaptobrevin family Q8TBG9
Transmembrane protein 45B (TMEM45B) TMEM45 family Q96B21
Amyloid protein-binding protein 2 (APPBP2) . Q92624
Chondrolectin (CHODL) . Q9H9P2
Fibronectin type III domain-containing protein 9 (FNDC9) . Q8TBE3
Transmembrane protein 80 (TMEM80) . Q96HE8

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
4 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570