Details of the Target

General Information of Target

Target ID LDTP12681
Target Name ATPase family AAA domain-containing protein 3A (ATAD3A)
Gene Name ATAD3A
Gene ID 55210
Synonyms
ATPase family AAA domain-containing protein 3A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEA
GTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGS
LVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSM
FKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSAL
DKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGD
SNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYV
STMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACK
LGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSK
VTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGF
LLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQAD
VRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISL
QEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELED
FELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKK
LSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMR
YAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEK
GKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIR
QFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSRE
DALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTA
APTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGT
LLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKL
SGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQY
EKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKE
PAKKKRKK
Target Bioclass
Enzyme
Family
AAA ATPase family
Subcellular location
Mitochondrion inner membrane
Function
Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis. Involved in mitochondrial-mediated antiviral innate immunity.
Uniprot ID
Q9NVI7
Ensemble ID
ENST00000378755.9
HGNC ID
HGNC:25567

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 25 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.77  LDD0402  [1]
CY-1
 Probe Info  		100.00  LDD0243  [2]
CY4
 Probe Info  		100.00  LDD0244  [2]
N1
 Probe Info  		100.00  LDD0242  [2]
TH211
 Probe Info  		Y540(20.00); Y399(9.56); Y559(5.35)  LDD0260  [3]
YN-1
 Probe Info  		100.00  LDD0444  [4]
STPyne
 Probe Info  		K262(4.35); K329(5.56); K406(10.00); K411(7.69)  LDD0277  [5]
BTD
 Probe Info  		C563(2.79)  LDD1699  [6]
AZ-9
 Probe Info  		E283(10.00)  LDD2209  [7]
ONAyne
 Probe Info  		N.A.  LDD0273  [5]
Probe 1
 Probe Info  		Y399(6.82)  LDD3495  [8]
ATP probe
 Probe Info  		K539(0.00); K46(0.00)  LDD0199  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [10]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [11]
IPM
 Probe Info  		N.A.  LDD0025  [12]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [12]
NHS
 Probe Info  		K549(0.00); K46(0.00)  LDD0010  [13]
SF
 Probe Info  		K472(0.00); K262(0.00); Y399(0.00); Y540(0.00)  LDD0028  [14]
1c-yne
 Probe Info  		K539(0.00); K194(0.00); K46(0.00); K72(0.00)  LDD0228  [15]
Acrolein
 Probe Info  		H613(0.00); H520(0.00)  LDD0217  [16]
Crotonaldehyde
 Probe Info  		H67(0.00); H520(0.00); K616(0.00); H613(0.00)  LDD0219  [16]
Methacrolein
 Probe Info  		N.A.  LDD0218  [16]
AOyne
 Probe Info  		12.80  LDD0443  [17]
TER-AC
 Probe Info  		N.A.  LDD0426  [18]
TPP-AC
 Probe Info  		N.A.  LDD0427  [18]
PAL-AfBPP Probe
Click To Hide/Show 57 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		7.84  LDD1728  [19]
C040
 Probe Info  		9.99  LDD1740  [19]
C055
 Probe Info  		15.24  LDD1752  [19]
C056
 Probe Info  		26.72  LDD1753  [19]
C070
 Probe Info  		8.51  LDD1766  [19]
C091
 Probe Info  		10.85  LDD1782  [19]
C092
 Probe Info  		18.00  LDD1783  [19]
C094
 Probe Info  		24.59  LDD1785  [19]
C106
 Probe Info  		21.41  LDD1793  [19]
C108
 Probe Info  		8.00  LDD1795  [19]
C112
 Probe Info  		21.86  LDD1799  [19]
C134
 Probe Info  		25.63  LDD1816  [19]
C135
 Probe Info  		10.13  LDD1817  [19]
C153
 Probe Info  		15.56  LDD1834  [19]
C169
 Probe Info  		20.53  LDD1849  [19]
C178
 Probe Info  		16.80  LDD1857  [19]
C201
 Probe Info  		30.70  LDD1877  [19]
C218
 Probe Info  		14.12  LDD1892  [19]
C220
 Probe Info  		13.93  LDD1894  [19]
C228
 Probe Info  		16.91  LDD1901  [19]
C231
 Probe Info  		18.77  LDD1904  [19]
C232
 Probe Info  		44.94  LDD1905  [19]
C233
 Probe Info  		8.69  LDD1906  [19]
C235
 Probe Info  		26.35  LDD1908  [19]
C238
 Probe Info  		12.13  LDD1911  [19]
C243
 Probe Info  		10.93  LDD1916  [19]
C246
 Probe Info  		13.18  LDD1919  [19]
C264
 Probe Info  		16.68  LDD1935  [19]
C278
 Probe Info  		59.71  LDD1948  [19]
C282
 Probe Info  		16.56  LDD1952  [19]
C285
 Probe Info  		20.53  LDD1955  [19]
C287
 Probe Info  		10.20  LDD1957  [19]
C289
 Probe Info  		34.06  LDD1959  [19]
C310
 Probe Info  		7.52  LDD1977  [19]
C349
 Probe Info  		10.34  LDD2010  [19]
C350
 Probe Info  		25.46  LDD2011  [19]
C355
 Probe Info  		23.75  LDD2016  [19]
C362
 Probe Info  		47.18  LDD2023  [19]
C363
 Probe Info  		19.56  LDD2024  [19]
C364
 Probe Info  		16.34  LDD2025  [19]
C388
 Probe Info  		46.21  LDD2047  [19]
C390
 Probe Info  		30.06  LDD2049  [19]
C407
 Probe Info  		12.04  LDD2064  [19]
FFF probe11
 Probe Info  		20.00  LDD0471  [20]
FFF probe13
 Probe Info  		20.00  LDD0475  [20]
FFF probe14
 Probe Info  		20.00  LDD0477  [20]
FFF probe15
 Probe Info  		8.86  LDD0478  [20]
FFF probe2
 Probe Info  		20.00  LDD0463  [20]
FFF probe3
 Probe Info  		20.00  LDD0464  [20]
FFF probe4
 Probe Info  		6.34  LDD0466  [20]
FFF probe6
 Probe Info  		6.26  LDD0467  [20]
JN0003
 Probe Info  		17.62  LDD0469  [20]
STS-1
 Probe Info  		4.90  LDD0136  [21]
STS-2
 Probe Info  		3.60  LDD0138  [21]
Dasatinib-CA-3PAP
 Probe Info  		4.33  LDD0365  [22]
DA-2
 Probe Info  		N.A.  LDD0071  [23]
OEA-DA
 Probe Info  		9.85  LDD0046  [24]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C563(1.08)  LDD2112  [6]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C563(0.56)  LDD2095  [6]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C563(0.99)  LDD2130  [6]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C563(1.15)  LDD2117  [6]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C563(1.34)  LDD2152  [6]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C563(0.96)  LDD2103  [6]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C563(0.54)  LDD2132  [6]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C563(0.83)  LDD2131  [6]
 LDCM0545  Acetamide MDA-MB-231 C563(0.36)  LDD2138  [6]
 LDCM0498  BS-3668 MDA-MB-231 C563(0.39)  LDD2091  [6]
 LDCM0108  Chloroacetamide HeLa H613(0.00); H520(0.00); H230(0.00)  LDD0222  [16]
 LDCM0097  Dasatinib K562 4.33  LDD0365  [22]
 LDCM0107  IAA HeLa H613(0.00); H520(0.00); H230(0.00)  LDD0221  [16]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C563(0.43)  LDD2121  [6]
 LDCM0109  NEM HeLa H520(0.00); H613(0.00); H347(0.00)  LDD0223  [16]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C563(0.51)  LDD2089  [6]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C563(0.88)  LDD2092  [6]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C563(0.93)  LDD2093  [6]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C563(1.10)  LDD2094  [6]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C563(0.36)  LDD2096  [6]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C563(0.79)  LDD2097  [6]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C563(1.11)  LDD2098  [6]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C563(0.86)  LDD2099  [6]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C563(1.03)  LDD2100  [6]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C563(0.52)  LDD2101  [6]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C563(0.89)  LDD2104  [6]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C563(1.42)  LDD2105  [6]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C563(0.89)  LDD2107  [6]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C563(0.77)  LDD2108  [6]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C563(0.76)  LDD2109  [6]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C563(0.84)  LDD2111  [6]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C563(0.47)  LDD2115  [6]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C563(0.63)  LDD2116  [6]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C563(1.03)  LDD2118  [6]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C563(2.37)  LDD2119  [6]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C563(0.94)  LDD2120  [6]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C563(0.67)  LDD2122  [6]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C563(0.83)  LDD2123  [6]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C563(1.13)  LDD2124  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C563(0.61)  LDD2125  [6]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C563(0.92)  LDD2127  [6]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C563(1.06)  LDD2128  [6]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C563(0.99)  LDD2129  [6]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C563(0.63)  LDD2133  [6]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C563(0.99)  LDD2134  [6]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C563(1.01)  LDD2135  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C563(1.07)  LDD2136  [6]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C563(1.23)  LDD1700  [6]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C563(0.62)  LDD2140  [6]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C563(0.86)  LDD2141  [6]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C563(1.06)  LDD2143  [6]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C563(2.54)  LDD2144  [6]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C563(0.72)  LDD2146  [6]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C563(3.23)  LDD2147  [6]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C563(0.63)  LDD2149  [6]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C563(0.31)  LDD2150  [6]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C563(0.59)  LDD2151  [6]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C563(1.97)  LDD2153  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
60 kDa heat shock protein, mitochondrial (HSPD1) Chaperonin (HSP60) family P10809
Dynamin-1-like protein (DNM1L) Dynamin/Fzo/YdjA family O00429

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
8 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
11 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
12 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
13 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
14 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
15 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
16 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
17 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
18 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
19 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
20 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
23 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
24 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570