Details of the Target

General Information of Target

Target ID LDTP03614
Target Name Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3)
Gene Name HNRNPH3
Gene ID 3189
Synonyms
HNRPH3; Heterogeneous nuclear ribonucleoprotein H3; hnRNP H3; Heterogeneous nuclear ribonucleoprotein 2H9; hnRNP 2H9
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MDWVMKHNGPNDASDGTVRLRGLPFGCSKEEIVQFFQGLEIVPNGITLTMDYQGRSTGEA
FVQFASKEIAENALGKHKERIGHRYIEIFRSSRSEIKGFYDPPRRLLGQRPGPYDRPIGG
RGGYYGAGRGSMYDRMRRGGDGYDGGYGGFDDYGGYNNYGYGNDGFDDRMRDGRGMGGHG
YGGAGDASSGFHGGHFVHMRGLPFRATENDIANFFSPLNPIRVHIDIGADGRATGEADVE
FVTHEDAVAAMSKDKNNMQHRYIELFLNSTPGGGSGMGGSGMGGYGRDGMDNQGGYGSVG
RMGMGNNYSGGYGTPDGLGGYGRGGGGSGGYYGQGGMSGGGWRGMY
Target Bioclass
Other
Subcellular location
Nucleus
Function
Involved in the splicing process and participates in early heat shock-induced splicing arrest. Due to their great structural variations the different isoforms may possess different functions in the splicing reaction.
Uniprot ID
P31942
Ensemble ID
ENST00000265866.12
HGNC ID
HGNC:5043

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-EBA
 Probe Info  		3.86  LDD0215  [1]
ILS-1
 Probe Info  		2.76  LDD0415  [2]
TH211
 Probe Info  		Y296(20.00); Y85(9.01); Y100(7.11); Y262(20.00)  LDD0260  [3]
C-Sul
 Probe Info  		2.41  LDD0066  [4]
TH216
 Probe Info  		Y296(20.00); Y85(12.38)  LDD0259  [3]
YN-1
 Probe Info  		100.00  LDD0444  [5]
STPyne
 Probe Info  		K255(10.00); K67(7.18); K97(3.53)  LDD0277  [6]
ONAyne
 Probe Info  		K97(0.00); K255(0.00)  LDD0273  [6]
Probe 1
 Probe Info  		Y296(63.26); Y312(14.49); Y332(7.93)  LDD3495  [7]
m-APA
 Probe Info  		11.35  LDD0403  [8]
P11
 Probe Info  		1.28  LDD0211  [9]
HPAP
 Probe Info  		3.77  LDD0063  [10]
5E-2FA
 Probe Info  		H7(0.00); H224(0.00)  LDD2235  [11]
ATP probe
 Probe Info  		N.A.  LDD0199  [12]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [13]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [14]
TFBX
 Probe Info  		N.A.  LDD0027  [15]
WYneO
 Probe Info  		N.A.  LDD0022  [16]
NHS
 Probe Info  		N.A.  LDD0010  [16]
SF
 Probe Info  		Y100(0.00); Y114(0.00); Y296(0.00)  LDD0028  [17]
1c-yne
 Probe Info  		K97(0.00); K6(0.00); K67(0.00)  LDD0228  [18]
Acrolein
 Probe Info  		H244(0.00); H7(0.00); H224(0.00)  LDD0217  [19]
Crotonaldehyde
 Probe Info  		H224(0.00); H7(0.00)  LDD0219  [19]
Methacrolein
 Probe Info  		N.A.  LDD0218  [19]
W1
 Probe Info  		N.A.  LDD0236  [20]
MPP-AC
 Probe Info  		N.A.  LDD0428  [21]
NAIA_5
 Probe Info  		N.A.  LDD2223  [22]
PAL-AfBPP Probe
Click To Hide/Show 60 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		6.23  LDD1714  [23]
C041
 Probe Info  		5.86  LDD1741  [23]
C063
 Probe Info  		11.63  LDD1760  [23]
C087
 Probe Info  		7.36  LDD1779  [23]
C106
 Probe Info  		18.51  LDD1793  [23]
C108
 Probe Info  		8.75  LDD1795  [23]
C112
 Probe Info  		18.00  LDD1799  [23]
C129
 Probe Info  		6.41  LDD1811  [23]
C145
 Probe Info  		8.22  LDD1827  [23]
C153
 Probe Info  		15.56  LDD1834  [23]
C159
 Probe Info  		7.89  LDD1839  [23]
C160
 Probe Info  		7.31  LDD1840  [23]
C161
 Probe Info  		14.22  LDD1841  [23]
C165
 Probe Info  		15.78  LDD1845  [23]
C187
 Probe Info  		19.29  LDD1865  [23]
C191
 Probe Info  		16.56  LDD1868  [23]
C193
 Probe Info  		5.66  LDD1869  [23]
C218
 Probe Info  		11.88  LDD1892  [23]
C220
 Probe Info  		14.62  LDD1894  [23]
C228
 Probe Info  		15.14  LDD1901  [23]
C232
 Probe Info  		60.13  LDD1905  [23]
C285
 Probe Info  		30.70  LDD1955  [23]
C286
 Probe Info  		5.46  LDD1956  [23]
C288
 Probe Info  		7.41  LDD1958  [23]
C289
 Probe Info  		37.01  LDD1959  [23]
C290
 Probe Info  		6.06  LDD1960  [23]
C313
 Probe Info  		16.22  LDD1980  [23]
C314
 Probe Info  		12.91  LDD1981  [23]
C322
 Probe Info  		6.87  LDD1988  [23]
C326
 Probe Info  		7.67  LDD1990  [23]
C338
 Probe Info  		23.10  LDD2001  [23]
C346
 Probe Info  		12.21  LDD2007  [23]
C348
 Probe Info  		13.18  LDD2009  [23]
C349
 Probe Info  		8.69  LDD2010  [23]
C350
 Probe Info  		23.75  LDD2011  [23]
C353
 Probe Info  		8.00  LDD2014  [23]
C355
 Probe Info  		29.45  LDD2016  [23]
C361
 Probe Info  		25.46  LDD2022  [23]
C380
 Probe Info  		4.99  LDD2039  [23]
C382
 Probe Info  		9.99  LDD2041  [23]
C383
 Probe Info  		11.96  LDD2042  [23]
C390
 Probe Info  		22.01  LDD2049  [23]
C391
 Probe Info  		15.24  LDD2050  [23]
C420
 Probe Info  		12.38  LDD2075  [23]
C431
 Probe Info  		14.32  LDD2086  [23]
FFF probe11
 Probe Info  		15.13  LDD0471  [24]
FFF probe12
 Probe Info  		6.26  LDD0473  [24]
FFF probe13
 Probe Info  		16.78  LDD0475  [24]
FFF probe14
 Probe Info  		13.03  LDD0477  [24]
FFF probe15
 Probe Info  		6.54  LDD0478  [24]
FFF probe2
 Probe Info  		20.00  LDD0463  [24]
FFF probe3
 Probe Info  		18.20  LDD0464  [24]
FFF probe4
 Probe Info  		13.10  LDD0466  [24]
FFF probe6
 Probe Info  		7.21  LDD0467  [24]
FFF probe7
 Probe Info  		6.22  LDD0483  [24]
JN0003
 Probe Info  		15.02  LDD0469  [24]
STS-2
 Probe Info  		N.A.  LDD0139  [25]
VE-P
 Probe Info  		N.A.  LDD0396  [26]
Photoindomethacin
 Probe Info  		E208(0.00); I211(0.00)  LDD0155  [27]
OEA-DA
 Probe Info  		8.97  LDD0046  [28]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.35  LDD0403  [8]
 LDCM0088  C45 HEK-293T 1.28  LDD0211  [9]
 LDCM0108  Chloroacetamide HeLa H195(0.00); H7(0.00); H244(0.00); H224(0.00)  LDD0222  [19]
 LDCM0107  IAA HeLa H244(0.00); H7(0.00); H224(0.00); H195(0.00)  LDD0221  [19]
 LDCM0109  NEM HeLa H244(0.00); H7(0.00); H224(0.00); H195(0.00)  LDD0223  [19]
 LDCM0014  Panhematin K562 3.77  LDD0063  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome activator complex subunit 3 (PSME3) PA28 family P61289
Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) . P09651
Spermatogenesis-associated protein 46 (SPATA46) . Q5T0L3

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Copper . DB09130

References

1 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
2 A Chemical Proteomic Analysis of Illudin-Interacting Proteins. Chemistry. 2019 Sep 25;25(54):12644-12651. doi: 10.1002/chem.201902919. Epub 2019 Sep 3.
Mass spectrometry data entry: PXD014175
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
8 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
9 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
10 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
11 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
12 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
13 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
14 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
15 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
16 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
17 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
18 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
19 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
20 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
21 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
22 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
23 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
24 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
25 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
26 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
27 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094
28 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570