Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C286  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 63 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12013  dCTP pyrophosphatase 1 (DCTPP1) 24.9
 LDTP02713  Macrophage migration inhibitory factor (MIF) 23.8
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 18.5
 LDTP08598  NAD-dependent protein deacetylase sirtuin-2 (SIRT2) 18.3
 LDTP03730  Sepiapterin reductase (SPR) 16.7
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 16.4
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 13.7
 LDTP08859  Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4) 13.6
 LDTP06227  Prostaglandin reductase 1 (PTGR1) 13.5
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 13.5
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 12.8
 LDTP04211  Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) 11.2
 LDTP04246  Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (FNTA) 11.2
 LDTP15422  Prostaglandin reductase 3 (PTGR3) 11.2
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 10.6
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 10.4
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 8.9
 LDTP04247  Protein farnesyltransferase subunit beta (FNTB) 8.8
 LDTP06618  ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) 8.7
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 8.3
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 8.2
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 7.9
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 7.9
 LDTP06200  Delta(14)-sterol reductase LBR (LBR) 7.8
 LDTP12197  Ethanolamine kinase 1 (ETNK1) 7.7
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 7.7
 LDTP10032  7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) 7.7
 LDTP03540  Heme oxygenase 2 (HMOX2) 7.6
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 7.6
 LDTP05480  Amidophosphoribosyltransferase (PPAT) 7.5
 LDTP06638  Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) 7.5
 LDTP05568  Pseudouridine-5'-phosphatase (PUDP) 7.5
 LDTP01333  Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) 7.4
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 7.3
 LDTP03060  Proteasome subunit beta type-1 (PSMB1) 7.2
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 7.1
 LDTP00886  Nardilysin (NRDC) 6.7
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 6.7
 LDTP13620  Lysosomal thioesterase PPT2 (PPT2) 6.7
 LDTP09666  Reticulon-4-interacting protein 1, mitochondrial (RTN4IP1) 6.6
 LDTP08790  Mitochondrial cardiolipin hydrolase (PLD6) 6.5
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 6.5
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 6.5
 LDTP13358  Leucyl-cystinyl aminopeptidase (LNPEP) 6.4
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 6.3
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 6.3
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 6.2
 LDTP03001  E3 ubiquitin-protein ligase TRIM21 (TRIM21) 6.1
 LDTP13751  Exonuclease 1 (EXO1) 6.1
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 6.1
 LDTP06645  Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) 5.8
 LDTP19745  ADP-ribosylation factor-like protein 10 (ARL10) 5.7
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 5.7
 LDTP01241  Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) 5.7
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 5.6
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 5.4
 LDTP09799  DCN1-like protein 4 (DCUN1D4) 5.4
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 5.3
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 5.2
 LDTP00697  Cytochrome b5 type B (CYB5B) 5.1
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 5.0
 LDTP05405  Mevalonate kinase (MVK) 5.0
 LDTP01769  Dihydrofolate reductase (DHFR) 5.0
Transporter and channel
Click to Show/Hide to Show 40 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP07236  Protein GPR107 (GPR107) 19.3
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 19.0
 LDTP15733  Solute carrier family 25 member 44 (SLC25A44) 16.3
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 15.8
 LDTP20002  Transmembrane protein 160 (TMEM160) 14.6
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 14.4
 LDTP10317  THO complex subunit 1 (THOC1) 10.6
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 10.1
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 10.1
 LDTP12383  Protein GPR108 (GPR108) 9.7
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 9.6
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 8.9
 LDTP07667  Transmembrane protein 205 (TMEM205) 8.2
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 8.1
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 8.0
 LDTP00262  Acetyl-coenzyme A transporter 1 (SLC33A1) 7.8
 LDTP15948  Transmembrane protein 126A (TMEM126A) 7.5
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 7.4
 LDTP04800  Nuclear pore complex protein Nup107 (NUP107) 7.3
 LDTP11844  Protein spinster homolog 1 (SPNS1) 7.3
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 7.2
 LDTP16813  Tetraspanin-3 (TSPAN3) 6.9
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 6.9
 LDTP08881  Transmembrane protein 199 (TMEM199) 6.8
 LDTP16163  Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial (COX16) 6.6
 LDTP03546  Translocator protein (TSPO) 6.5
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 6.3
 LDTP10931  Succinate dehydrogenase cytochrome b560 subunit, mitochondrial (SDHC) 6.0
 LDTP15481  Transmembrane protein 87A (TMEM87A) 5.9
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 5.9
 LDTP06275  Lysosomal-associated transmembrane protein 4A (LAPTM4A) 5.9
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 5.8
 LDTP02215  Prosaposin (PSAP) 5.8
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 5.6
 LDTP11869  Growth hormone-inducible transmembrane protein (GHITM) 5.4
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 5.2
 LDTP00541  NPC intracellular cholesterol transporter 1 (NPC1) 5.2
 LDTP04597  Methylosome subunit pICln (CLNS1A) 5.1
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 5.1
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 5.0
Other
Click to Show/Hide to Show 42 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP18561  Small ribosomal subunit protein mS33 (MRPS33) 70.0
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 63.1
 LDTP10381  Peroxisomal membrane protein 11C (PEX11G) 19.8
 LDTP06892  Protein Hikeshi (HIKESHI) 15.8
 LDTP16006  Optic atrophy 3 protein (OPA3) 15.5
 LDTP06068  MAGUK p55 subfamily member 2 (MPP2) 13.9
 LDTP00417  Programmed cell death protein 5 (PDCD5) 10.8
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 10.3
 LDTP14250  Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) 10.1
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 9.4
 LDTP05160  Nucleobindin-2 (NUCB2) 8.6
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 8.5
 LDTP11871  Cobalamin trafficking protein CblD (MMADHC) 8.3
 LDTP07345  Cell division cycle-associated protein 2 (CDCA2) 8.2
 LDTP16156  Protein PALS2 (PALS2) 8.0
 LDTP01075  Protein CutA (CUTA) 7.9
 LDTP00105  Protein unc-119 homolog B (UNC119B) 7.8
 LDTP07335  Rho GTPase-activating protein 17 (ARHGAP17) 7.5
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 7.5
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 7.3
 LDTP12737  BRISC and BRCA1-A complex member 1 (BABAM1) 6.9
 LDTP00850  ER lumen protein-retaining receptor 3 (KDELR3) 6.8
 LDTP15915  Large ribosomal subunit protein uL13m (MRPL13) 6.6
 LDTP08689  Dyslexia-associated protein KIAA0319-like protein (KIAA0319L) 6.5
 LDTP13430  Protein TASOR (TASOR) 6.5
 LDTP12746  Gem-associated protein 8 (GEMIN8) 6.4
 LDTP03775  RNA-binding protein FUS (FUS) 6.3
 LDTP08594  Negative elongation factor C/D (NELFCD) 6.2
 LDTP11978  HAUS augmin-like complex subunit 4 (HAUS4) 6.0
 LDTP02345  Retinol-binding protein 1 (RBP1) 5.7
 LDTP00500  Heterogeneous nuclear ribonucleoprotein D-like (HNRNPDL) 5.6
 LDTP03614  Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3) 5.5
 LDTP11076  Apolipoprotein L2 (APOL2) 5.4
 LDTP05960  Trophoblast glycoprotein (TPBG) 5.3
 LDTP15714  Mdm2-binding protein (MTBP) 5.2
 LDTP15634  Ubiquinol-cytochrome c reductase complex assembly factor 5 (UQCC5) 5.2
 LDTP15623  Neuferricin (CYB5D2) 5.2
 LDTP09881  TATA-binding protein-associated factor 2N (TAF15) 5.1
 LDTP11611  Centrosomal protein of 44 kDa (CEP44) 5.0
 LDTP08094  Wings apart-like protein homolog (WAPL) 5.0
 LDTP06948  Protein YIF1B (YIF1B) 5.0
 LDTP09970  RNA-binding protein with multiple splicing (RBPMS) 5.0

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587