Details of the Target

General Information of Target

Target ID LDTP05568
Target Name Pseudouridine-5'-phosphatase (PUDP)
Gene Name PUDP
Gene ID 8226
Synonyms
DXF68S1E; FAM16AX; GS1; HDHD1; HDHD1A; Pseudouridine-5'-phosphatase; EC 3.1.3.96; Haloacid dehalogenase-like hydrolase domain-containing protein 1; Haloacid dehalogenase-like hydrolase domain-containing protein 1A; Protein GS1; Pseudouridine-5'-monophosphatase; 5'-PsiMPase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPPQPVTHLIFDMDGLLLDTERLYSVVFQEICNRYDKKYSWDVKSLVMGKKALEAAQI
IIDVLQLPMSKEELVEESQTKLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSGSASF
DMKTSRHKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNG
VEAALAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE
Target Bioclass
Enzyme
Family
HAD-like hydrolase superfamily, CbbY/CbbZ/Gph/YieH family
Function Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine.
Uniprot ID
Q08623
Ensemble ID
ENST00000381077.10
HGNC ID
HGNC:16818

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.E23K; p.P225S .
OCUG1 SNV: p.M1? .
SNU1 SNV: p.M193K .
SW1573 SNV: p.K123N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K46(5.79); K83(8.26)  LDD0277  [1]
HHS-475
 Probe Info  		Y26(1.15)  LDD0264  [2]
HHS-465
 Probe Info  		Y26(10.00)  LDD2237  [3]
DBIA
 Probe Info  		C171(1.13)  LDD1508  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		C34(0.00); C171(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C34(0.00); C171(0.00)  LDD0037  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
NAIA_5
 Probe Info  		C171(0.00); C158(0.00); C34(0.00)  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C286
 Probe Info  		7.46  LDD1956  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C171(1.13)  LDD1508  [4]
 LDCM0277  AC18 HEK-293T C171(1.00)  LDD1516  [4]
 LDCM0279  AC2 HEK-293T C171(1.14)  LDD1518  [4]
 LDCM0286  AC26 HEK-293T C171(1.30)  LDD1525  [4]
 LDCM0295  AC34 HEK-293T C171(0.92)  LDD1534  [4]
 LDCM0304  AC42 HEK-293T C171(0.87)  LDD1543  [4]
 LDCM0313  AC50 HEK-293T C171(1.14)  LDD1552  [4]
 LDCM0321  AC58 HEK-293T C171(1.13)  LDD1560  [4]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0369  CL100 HEK-293T C171(0.93)  LDD1573  [4]
 LDCM0373  CL104 HEK-293T C171(1.11)  LDD1577  [4]
 LDCM0377  CL108 HEK-293T C171(1.30)  LDD1581  [4]
 LDCM0382  CL112 HEK-293T C171(0.94)  LDD1586  [4]
 LDCM0386  CL116 HEK-293T C171(1.06)  LDD1590  [4]
 LDCM0391  CL120 HEK-293T C171(1.30)  LDD1595  [4]
 LDCM0395  CL124 HEK-293T C171(0.96)  LDD1599  [4]
 LDCM0399  CL128 HEK-293T C171(1.06)  LDD1603  [4]
 LDCM0403  CL16 HEK-293T C171(1.31)  LDD1607  [4]
 LDCM0405  CL18 HEK-293T C171(1.01)  LDD1609  [4]
 LDCM0416  CL28 HEK-293T C171(0.94)  LDD1620  [4]
 LDCM0419  CL30 HEK-293T C171(1.22)  LDD1623  [4]
 LDCM0429  CL4 HEK-293T C171(1.07)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C171(0.92)  LDD1634  [4]
 LDCM0432  CL42 HEK-293T C171(1.02)  LDD1636  [4]
 LDCM0443  CL52 HEK-293T C171(1.29)  LDD1646  [4]
 LDCM0445  CL54 HEK-293T C171(1.18)  LDD1648  [4]
 LDCM0451  CL6 HEK-293T C171(0.94)  LDD1654  [4]
 LDCM0456  CL64 HEK-293T C171(1.16)  LDD1659  [4]
 LDCM0458  CL66 HEK-293T C171(1.08)  LDD1661  [4]
 LDCM0469  CL76 HEK-293T C171(0.97)  LDD1672  [4]
 LDCM0471  CL78 HEK-293T C171(1.13)  LDD1674  [4]
 LDCM0482  CL88 HEK-293T C171(0.92)  LDD1685  [4]
 LDCM0485  CL90 HEK-293T C171(0.87)  LDD1688  [4]
 LDCM0576  Fragment14 Ramos C34(0.54)  LDD2193  [9]
 LDCM0566  Fragment4 Ramos C34(0.80)  LDD2184  [9]
 LDCM0569  Fragment7 Ramos C34(0.38)  LDD2186  [9]
 LDCM0116  HHS-0101 DM93 Y26(1.15)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y26(1.70)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y26(1.45)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y26(2.00)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y26(1.29)  LDD0268  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0022  KB02 Ramos C34(0.26)  LDD2182  [9]
 LDCM0023  KB03 Ramos C34(0.30)  LDD2183  [9]
 LDCM0024  KB05 Ramos C34(0.24)  LDD2185  [9]
 LDCM0109  NEM HeLa N.A.  LDD0224  [6]
 LDCM0131  RA190 MM1.R C34(1.12)  LDD0304  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glycerate kinase (GLYCTK) Glycerate kinase type-2 family Q8IVS8
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AFG2-interacting ribosome maturation factor (AIRIM) . Q9NX04
Melanoma-associated antigen 4 (MAGEA4) . P43358

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
10 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.