Details of the Target

General Information of Target

Target ID LDTP09666
Target Name Reticulon-4-interacting protein 1, mitochondrial (RTN4IP1)
Gene Name RTN4IP1
Gene ID 84816
Synonyms
NIMP; Reticulon-4-interacting protein 1, mitochondrial; NOGO-interacting mitochondrial protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFT
QNMMMPIIHYPNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLG
RDVSGVVMECGLDVKYFKPGDEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAAS
LPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDA
SELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLKKWSGATYVTL
VTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIAELVDAG
KIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV
Target Bioclass
Enzyme
Family
Zinc-containing alcohol dehydrogenase family, Quinone oxidoreductase subfamily
Subcellular location
Mitochondrion outer membrane
Function
Plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury.
Uniprot ID
Q8WWV3
Ensemble ID
ENST00000369063.8
HGNC ID
HGNC:18647

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HKGZCC SNV: p.F19V .
NCIH1155 SNV: p.A80T DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C349(1.34)  LDD3312  [1]
BTD
 Probe Info  		C203(0.78)  LDD2099  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		C236(0.00); C349(0.00)  LDD0037  [3]
STPyne
 Probe Info  		N.A.  LDD0009  [4]
NAIA_5
 Probe Info  		C130(0.00); C203(0.00)  LDD2223  [5]
HHS-465
 Probe Info  		N.A.  LDD2240  [6]
PAL-AfBPP Probe
Click To Hide/Show 45 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		13.55  LDD1740  [7]
C052
 Probe Info  		5.82  LDD1750  [7]
C056
 Probe Info  		22.01  LDD1753  [7]
C091
 Probe Info  		18.77  LDD1782  [7]
C092
 Probe Info  		83.29  LDD1783  [7]
C094
 Probe Info  		38.59  LDD1785  [7]
C112
 Probe Info  		23.92  LDD1799  [7]
C139
 Probe Info  		13.64  LDD1821  [7]
C140
 Probe Info  		21.41  LDD1822  [7]
C158
 Probe Info  		12.73  LDD1838  [7]
C161
 Probe Info  		67.65  LDD1841  [7]
C166
 Probe Info  		7.67  LDD1846  [7]
C169
 Probe Info  		42.22  LDD1849  [7]
C174
 Probe Info  		21.11  LDD1854  [7]
C185
 Probe Info  		5.28  LDD1863  [7]
C186
 Probe Info  		15.03  LDD1864  [7]
C187
 Probe Info  		48.50  LDD1865  [7]
C201
 Probe Info  		54.95  LDD1877  [7]
C206
 Probe Info  		35.75  LDD1881  [7]
C210
 Probe Info  		61.82  LDD1884  [7]
C218
 Probe Info  		15.45  LDD1892  [7]
C219
 Probe Info  		6.54  LDD1893  [7]
C220
 Probe Info  		24.08  LDD1894  [7]
C228
 Probe Info  		28.84  LDD1901  [7]
C232
 Probe Info  		68.12  LDD1905  [7]
C235
 Probe Info  		71.51  LDD1908  [7]
C246
 Probe Info  		18.13  LDD1919  [7]
C265
 Probe Info  		17.15  LDD1936  [7]
C270
 Probe Info  		20.82  LDD1940  [7]
C278
 Probe Info  		56.49  LDD1948  [7]
C280
 Probe Info  		11.24  LDD1950  [7]
C286
 Probe Info  		6.63  LDD1956  [7]
C287
 Probe Info  		16.45  LDD1957  [7]
C289
 Probe Info  		34.78  LDD1959  [7]
C322
 Probe Info  		6.77  LDD1988  [7]
C339
 Probe Info  		25.46  LDD2002  [7]
C379
 Probe Info  		6.73  LDD2038  [7]
C403
 Probe Info  		35.75  LDD2061  [7]
C407
 Probe Info  		13.27  LDD2064  [7]
C426
 Probe Info  		55.72  LDD2081  [7]
FFF probe11
 Probe Info  		20.00  LDD0471  [8]
FFF probe14
 Probe Info  		19.66  LDD0477  [8]
FFF probe2
 Probe Info  		20.00  LDD0463  [8]
JN0003
 Probe Info  		20.00  LDD0469  [8]
A-DA
 Probe Info  		2.14  LDD0142  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C349(1.13)  LDD1507  [10]
 LDCM0259  AC14 HEK-293T C349(0.93)  LDD1512  [10]
 LDCM0276  AC17 HEK-293T C349(1.45)  LDD1515  [10]
 LDCM0281  AC21 HEK-293T C349(0.92)  LDD1520  [10]
 LDCM0282  AC22 HEK-293T C349(0.94)  LDD1521  [10]
 LDCM0285  AC25 HEK-293T C349(1.33)  LDD1524  [10]
 LDCM0289  AC29 HEK-293T C349(1.00)  LDD1528  [10]
 LDCM0291  AC30 HEK-293T C349(1.05)  LDD1530  [10]
 LDCM0294  AC33 HEK-293T C349(1.34)  LDD1533  [10]
 LDCM0298  AC37 HEK-293T C349(1.04)  LDD1537  [10]
 LDCM0299  AC38 HEK-293T C349(1.01)  LDD1538  [10]
 LDCM0303  AC41 HEK-293T C349(1.06)  LDD1542  [10]
 LDCM0307  AC45 HEK-293T C349(0.93)  LDD1546  [10]
 LDCM0308  AC46 HEK-293T C349(0.97)  LDD1547  [10]
 LDCM0311  AC49 HEK-293T C349(1.20)  LDD1550  [10]
 LDCM0312  AC5 HEK-293T C349(0.92)  LDD1551  [10]
 LDCM0316  AC53 HEK-293T C349(0.93)  LDD1555  [10]
 LDCM0317  AC54 HEK-293T C349(1.02)  LDD1556  [10]
 LDCM0320  AC57 HEK-293T C349(1.14)  LDD1559  [10]
 LDCM0323  AC6 HEK-293T C349(0.94)  LDD1562  [10]
 LDCM0325  AC61 HEK-293T C349(0.96)  LDD1564  [10]
 LDCM0326  AC62 HEK-293T C349(1.05)  LDD1565  [10]
 LDCM0248  AKOS034007472 HEK-293T C349(1.00)  LDD1511  [10]
 LDCM0356  AKOS034007680 HEK-293T C349(1.33)  LDD1570  [10]
 LDCM0083  Avasimibe A-549 7.68  LDD0143  [9]
 LDCM0368  CL10 HEK-293T C349(0.81)  LDD1572  [10]
 LDCM0404  CL17 HEK-293T C349(1.04)  LDD1608  [10]
 LDCM0409  CL21 HEK-293T C349(0.85)  LDD1613  [10]
 LDCM0410  CL22 HEK-293T C349(1.08)  LDD1614  [10]
 LDCM0417  CL29 HEK-293T C349(1.14)  LDD1621  [10]
 LDCM0422  CL33 HEK-293T C349(0.76)  LDD1626  [10]
 LDCM0423  CL34 HEK-293T C349(0.85)  LDD1627  [10]
 LDCM0431  CL41 HEK-293T C349(1.15)  LDD1635  [10]
 LDCM0435  CL45 HEK-293T C349(0.91)  LDD1639  [10]
 LDCM0436  CL46 HEK-293T C349(0.96)  LDD1640  [10]
 LDCM0440  CL5 HEK-293T C349(1.14)  LDD1644  [10]
 LDCM0444  CL53 HEK-293T C349(1.07)  LDD1647  [10]
 LDCM0448  CL57 HEK-293T C349(0.82)  LDD1651  [10]
 LDCM0449  CL58 HEK-293T C349(1.02)  LDD1652  [10]
 LDCM0457  CL65 HEK-293T C349(1.16)  LDD1660  [10]
 LDCM0461  CL69 HEK-293T C349(1.01)  LDD1664  [10]
 LDCM0463  CL70 HEK-293T C349(0.94)  LDD1666  [10]
 LDCM0470  CL77 HEK-293T C349(0.93)  LDD1673  [10]
 LDCM0475  CL81 HEK-293T C349(0.92)  LDD1678  [10]
 LDCM0476  CL82 HEK-293T C349(0.97)  LDD1679  [10]
 LDCM0483  CL89 HEK-293T C349(1.22)  LDD1686  [10]
 LDCM0484  CL9 HEK-293T C349(0.98)  LDD1687  [10]
 LDCM0488  CL93 HEK-293T C349(0.89)  LDD1691  [10]
 LDCM0489  CL94 HEK-293T C349(0.98)  LDD1692  [10]
 LDCM0185  Compound 17 HEK-293T 4.26  LDD0512  [8]
 LDCM0625  F8 Ramos C203(4.67)  LDD2187  [11]
 LDCM0082  FK866 A-549 2.14  LDD0142  [9]
 LDCM0572  Fragment10 Ramos C203(1.66)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C203(6.23)  LDD2190  [11]
 LDCM0574  Fragment12 Ramos C203(2.17)  LDD2191  [11]
 LDCM0579  Fragment20 Ramos C203(1.57)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C203(0.91)  LDD2195  [11]
 LDCM0586  Fragment28 Ramos C203(0.61)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C203(1.81)  LDD2199  [11]
 LDCM0590  Fragment32 Ramos C203(1.72)  LDD2201  [11]
 LDCM0610  Fragment52 Ramos C203(1.42)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C203(2.83)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C203(2.19)  LDD2186  [11]
 LDCM0571  Fragment9 Ramos C203(1.60)  LDD2188  [11]
 LDCM0022  KB02 Ramos C203(1.66)  LDD2182  [11]
 LDCM0023  KB03 Ramos C203(2.27)  LDD2183  [11]
 LDCM0024  KB05 HMCB C349(1.34)  LDD3312  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C203(0.78)  LDD2099  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C203(0.72)  LDD2107  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C203(0.96)  LDD2146  [2]
 LDCM0084  Ro 48-8071 A-549 2.42  LDD0145  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase MARCHF5 (MARCHF5) . Q9NX47
E3 ubiquitin-protein ligase SMURF2 (SMURF2) . Q9HAU4
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Splicing factor, arginine/serine-rich 19 (SCAF1) Splicing factor SR family Q9H7N4
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Highly divergent homeobox (HDX) . Q7Z353
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Centromere protein K (CENPK) CENP-K/MCM22 family Q9BS16
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
ADP-ribosylation factor-binding protein GGA2 (GGA2) GGA protein family Q9UJY4
Partner of Y14 and mago (PYM1) Pym family Q9BRP8
Zyxin (ZYX) Zyxin/ajuba family Q15942
ADP-ribosylation factor GTPase-activating protein 3 (ARFGAP3) . Q9NP61
Ermin (ERMN) . Q8TAM6
WW domain-containing transcription regulator protein 1 (WWTR1) . Q9GZV5

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
9 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578