Details of the Target

General Information of Target

Target ID LDTP07156
Target Name Protein wntless homolog (WLS)
Gene Name WLS
Gene ID 79971
Synonyms
C1orf139; GPR177; Protein wntless homolog; Integral membrane protein GPR177; Protein evenness interrupted homolog; EVI; Putative NF-kappa-B-activating protein 373
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIAPGPTTAVSYMSVKCVDARKNHHKT
KWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAF
KLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECD
VLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTF
LTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFG
DIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQ
LTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKV
RRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGM
WNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQ
E
Target Bioclass
Transporter and channel
Family
Wntless family
Subcellular location
Golgi apparatus membrane
Function
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
Uniprot ID
Q5T9L3
Ensemble ID
ENST00000262348.9
HGNC ID
HGNC:30238

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ICC12 SNV: p.K419N DBIA    Probe Info 
IPC298 SNV: p.I373T DBIA    Probe Info 
JM1 SNV: p.L349V .
NCIH661 SNV: p.P263A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K12(6.25)  LDD0277  [1]
OPA-S-S-alkyne
 Probe Info  		K217(2.13); K168(5.48); K161(9.24)  LDD3494  [2]
DBIA
 Probe Info  		C71(3.65)  LDD3311  [3]
BTD
 Probe Info  		C162(2.19)  LDD1700  [4]
ATP probe
 Probe Info  		K73(0.00); K12(0.00); K13(0.00)  LDD0199  [5]
IA-alkyne
 Probe Info  		C162(0.00); C179(0.00); C71(0.00)  LDD0165  [6]
IPM
 Probe Info  		C162(0.00); C71(0.00)  LDD0005  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
AOyne
 Probe Info  		13.10  LDD0443  [9]
PAL-AfBPP Probe
Click To Hide/Show 51 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C017
 Probe Info  		8.94  LDD1725  [10]
C040
 Probe Info  		12.04  LDD1740  [10]
C041
 Probe Info  		5.82  LDD1741  [10]
C055
 Probe Info  		14.52  LDD1752  [10]
C056
 Probe Info  		20.97  LDD1753  [10]
C063
 Probe Info  		21.56  LDD1760  [10]
C070
 Probe Info  		16.91  LDD1766  [10]
C072
 Probe Info  		12.82  LDD1768  [10]
C106
 Probe Info  		28.44  LDD1793  [10]
C107
 Probe Info  		12.04  LDD1794  [10]
C108
 Probe Info  		15.89  LDD1795  [10]
C112
 Probe Info  		26.17  LDD1799  [10]
C141
 Probe Info  		12.30  LDD1823  [10]
C143
 Probe Info  		19.56  LDD1825  [10]
C147
 Probe Info  		8.17  LDD1829  [10]
C159
 Probe Info  		9.58  LDD1839  [10]
C160
 Probe Info  		10.48  LDD1840  [10]
C161
 Probe Info  		13.36  LDD1841  [10]
C187
 Probe Info  		17.03  LDD1865  [10]
C220
 Probe Info  		12.21  LDD1894  [10]
C225
 Probe Info  		5.54  LDD1898  [10]
C226
 Probe Info  		5.62  LDD1899  [10]
C229
 Probe Info  		24.08  LDD1902  [10]
C231
 Probe Info  		46.21  LDD1904  [10]
C232
 Probe Info  		75.06  LDD1905  [10]
C233
 Probe Info  		34.30  LDD1906  [10]
C234
 Probe Info  		28.84  LDD1907  [10]
C235
 Probe Info  		59.71  LDD1908  [10]
C243
 Probe Info  		25.81  LDD1916  [10]
C244
 Probe Info  		39.67  LDD1917  [10]
C245
 Probe Info  		8.00  LDD1918  [10]
C246
 Probe Info  		38.05  LDD1919  [10]
C249
 Probe Info  		33.13  LDD1922  [10]
C264
 Probe Info  		18.25  LDD1935  [10]
C287
 Probe Info  		10.48  LDD1957  [10]
C288
 Probe Info  		6.36  LDD1958  [10]
C292
 Probe Info  		5.62  LDD1962  [10]
C293
 Probe Info  		40.79  LDD1963  [10]
C296
 Probe Info  		56.89  LDD1966  [10]
C297
 Probe Info  		7.52  LDD1967  [10]
C313
 Probe Info  		13.64  LDD1980  [10]
C314
 Probe Info  		15.56  LDD1981  [10]
C317
 Probe Info  		6.59  LDD1983  [10]
C382
 Probe Info  		9.06  LDD2041  [10]
FFF probe11
 Probe Info  		20.00  LDD0471  [11]
FFF probe13
 Probe Info  		20.00  LDD0475  [11]
FFF probe14
 Probe Info  		20.00  LDD0477  [11]
FFF probe15
 Probe Info  		14.30  LDD0478  [11]
FFF probe3
 Probe Info  		20.00  LDD0464  [11]
FFF probe6
 Probe Info  		9.80  LDD0467  [11]
OEA-DA
 Probe Info  		20.00  LDD0046  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0498  BS-3668 MDA-MB-231 C162(0.43)  LDD2091  [4]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C162(2.80)  LDD1702  [4]
 LDCM0107  IAA HeLa H153(0.00); H192(0.00)  LDD0221  [8]
 LDCM0022  KB02 HEK-293T C162(0.88); C71(0.95); C179(0.95)  LDD1492  [13]
 LDCM0023  KB03 HEK-293T C162(0.95); C71(0.99); C179(0.97)  LDD1497  [13]
 LDCM0024  KB05 G361 C71(3.65)  LDD3311  [3]
 LDCM0109  NEM HeLa N.A.  LDD0225  [8]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C162(2.19)  LDD1700  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C162(1.87)  LDD2144  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endoplasmic reticulum membrane protein complex subunit 7 (EMC7) EMC7 family Q9NPA0
Aquaporin-4 (AQP4) MIP/aquaporin (TC 1.A.8) family P55087
Sodium-dependent dopamine transporter (SLC6A3) Sodium:neurotransmitter symporter (SNF) family Q01959
GPCR
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Adenosine receptor A2a (ADORA2A) G-protein coupled receptor 1 family P29274
Mu-type opioid receptor (OPRM1) G-protein coupled receptor 1 family P35372
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Neuronal membrane glycoprotein M6-a (GPM6A) Myelin proteolipid protein family P51674
Protein Wnt-3a (WNT3A) Wnt family P56704
SH3-containing GRB2-like protein 3-interacting protein 1 (SGIP1) . Q9BQI5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
12 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
13 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402