Details of the Probe

General Information of Probe

Probe ID
LDPC0462
Probe Name
C229
Synonyms
Z4225926264
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 327.4
Lipid-water partition coefficient (xlogp) 2.4
Rotatable Bond Count (rotbonds) 7
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 5
Chemical Identifiers
Formula
C18H21N3O3
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-6-methoxy-3,4-dihydro-2H-chromene-3-carboxamide
Canonical SMILES
COC1=CC2=C(C=C1)OCC(C2)C(=O)NCCC3(N=N3)CCC#C
InChI
InChI=1S/C18H21N3O3/c1-3-4-7-18(20-21-18)8-9-19-17(22)14-10-13-11-15(23-2)5-6-16(13)24-12-14/h1,5-6,11,14H,4,7-10,12H2,2H3,(H,19,22)
InChIKey
OWOWVWFPQZENAI-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

84 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-ketodihydrosphingosine reductase (KDSR) LDTP05476 13.64  LDD1902 
5-hydroxymethyl-dUMP N-hydrolase (DNPH1) LDTP00813 9.32  LDD1902 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 14.83  LDD1902 
Acid ceramidase (ASAH1) LDTP05905 9.58  LDD1902 
Adenosine kinase (ADK) LDTP04689 28.84  LDD1902 
ADP-ribosylation factor-like protein 10 (ARL10) LDTP19745 12.55  LDD1902 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 15.56  LDD1902 
Atlastin-2 (ATL2) LDTP09251 8.88  LDD1902 
ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) LDTP06618 30.70  LDD1902 
Atypical kinase COQ8A, mitochondrial (COQ8A) LDTP09285 15.35  LDD1902 
Atypical kinase COQ8B, mitochondrial (COQ8B) LDTP10201 7.11  LDD1902 
Beta-galactosidase (GLB1) LDTP02837 13.27  LDD1902 
Beta-hexosaminidase subunit alpha (HEXA) LDTP02169 7.94  LDD1902 
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 7.78  LDD1902 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 8.57  LDD1902 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 8.82  LDD1902 
Carboxypeptidase D (CPD) LDTP01372 7.06  LDD1902 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 17.15  LDD1902 
CDGSH iron-sulfur domain-containing protein 1 (CISD1) LDTP12873 11.39  LDD1902 
Ceramide synthase 2 (CERS2) LDTP10329 7.01  LDD1902 
Cytochrome b-c1 complex subunit 8 (UQCRQ) LDTP00486 13.64  LDD1902 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 14.93  LDD1902 
Cytochrome b5 type B (CYB5B) LDTP00697 8.94  LDD1902 
Cytochrome P450 20A1 (CYP20A1) LDTP17410 7.73  LDD1902 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 13.18  LDD1902 
Delta(14)-sterol reductase LBR (LBR) LDTP06200 10.27  LDD1902 
Delta(14)-sterol reductase TM7SF2 (TM7SF2) LDTP01389 7.46  LDD1902 
Delta(24)-sterol reductase (DHCR24) LDTP06375 11.47  LDD1902 
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) LDTP05724 10.34  LDD1902 
DNA replication licensing factor MCM4 (MCM4) LDTP03680 43.41  LDD1902 
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A) LDTP04120 13.18  LDD1902 
Dolichyldiphosphatase 1 (DOLPP1) LDTP15328 10.63  LDD1902 
Endoplasmic reticulum metallopeptidase 1 (ERMP1) LDTP08017 29.04  LDD1902 
Endoplasmic reticulum protein SC65 (P3H4) LDTP09874 7.46  LDD1902 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 9.51  LDD1902 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 8.94  LDD1902 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 7.36  LDD1902 
Ferrochelatase, mitochondrial (FECH) LDTP03198 15.67  LDD1902 
Glyoxalase domain-containing protein 4 (GLOD4) LDTP16040 8.00  LDD1902 
Heme A synthase COX15 (COX15) LDTP07931 8.00  LDD1902 
Heme oxygenase 1 (HMOX1) LDTP02359 7.62  LDD1902 
Heme oxygenase 2 (HMOX2) LDTP03540 33.82  LDD1902 
Holocytochrome c-type synthase (HCCS) LDTP04581 11.79  LDD1902 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 8.88  LDD1902 
Lanosterol synthase (LSS) LDTP04183 7.01  LDD1902 
Leucyl-cystinyl aminopeptidase (LNPEP) LDTP13358 7.16  LDD1902 
Leukotriene A-4 hydrolase (LTA4H) LDTP02385 12.13  LDD1902 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 14.93  LDD1902 
Lysosomal acid glucosylceramidase (GBA1) LDTP02005 9.38  LDD1902 
Lysosomal acid phosphatase (ACP2) LDTP02539 8.82  LDD1902 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 16.00  LDD1902 
Lysosomal protective protein (CTSA) LDTP02505 13.55  LDD1902 
Lysosomal thioesterase PPT2 (PPT2) LDTP13620 9.58  LDD1902 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 13.00  LDD1902 
Minor histocompatibility antigen H13 (HM13) LDTP09396 9.25  LDD1902 
NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) LDTP08385 9.38  LDD1902 
NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) LDTP12959 7.21  LDD1902 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 17.03  LDD1902 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 11.31  LDD1902 
NADPH--cytochrome P450 reductase (POR) LDTP02848 7.36  LDD1902 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 19.29  LDD1902 
Palmitoyltransferase ZDHHC13 (ZDHHC13) LDTP08455 8.57  LDD1902 
Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) LDTP05029 12.91  LDD1902 
Phospholipase A2 group XV (PLA2G15) LDTP09088 7.94  LDD1902 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 17.27  LDD1902 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 15.24  LDD1902 
Protein O-GlcNAcase (OGA) LDTP00988 7.67  LDD1902 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 24.42  LDD1902 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 13.18  LDD1902 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 17.15  LDD1902 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 15.67  LDD1902 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 14.42  LDD1902 
Ribosomal protein S6 kinase alpha-3 (RPS6KA3) LDTP04471 11.08  LDD1902 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 9.78  LDD1902 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 10.85  LDD1902 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 13.18  LDD1902 
Sphingomyelin phosphodiesterase (SMPD1) LDTP02896 14.52  LDD1902 
Sphingosine-1-phosphate lyase 1 (SGPL1) LDTP01613 8.22  LDD1902 
Sulfhydryl oxidase 2 (QSOX2) LDTP07813 7.16  LDD1902 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 8.88  LDD1902 
V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) LDTP14030 10.20  LDD1902 
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) LDTP08728 16.56  LDD1902 
Vitamin K-dependent gamma-carboxylase (GGCX) LDTP03854 11.39  LDD1902 
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) LDTP06320 7.73  LDD1902 
------------------------------------------------------------------------------------
⏷ Show the Full List of 84 Enzyme
71 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) LDTP01601 7.26  LDD1902 
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) LDTP09328 13.64  LDD1902 
ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) LDTP03284 17.51  LDD1902 
ATP synthase subunit a (MT-ATP6) LDTP01804 23.59  LDD1902 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 14.93  LDD1902 
BRI3-binding protein (BRI3BP) LDTP15648 16.68  LDD1902 
Complex I assembly factor TMEM126B, mitochondrial (TMEM126B) LDTP08469 7.78  LDD1902 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 18.51  LDD1902 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 16.45  LDD1902 
ER membrane protein complex subunit 5 (MMGT1) LDTP08878 18.90  LDD1902 
Essential MCU regulator, mitochondrial (SMDT1) LDTP11927 15.45  LDD1902 
H(+)/Cl(-) exchange transporter 7 (CLCN7) LDTP04463 10.85  LDD1902 
Integral membrane protein 2B (ITM2B) LDTP13833 13.18  LDD1902 
Lysosome membrane protein 2 (SCARB2) LDTP06034 23.59  LDD1902 
Magnesium transporter NIPA3 (NIPAL1) LDTP17362 18.64  LDD1902 
Major facilitator superfamily domain-containing protein 8 (MFSD8) LDTP09261 20.11  LDD1902 
Melanoma inhibitory activity protein 2 (MIA2) LDTP10669 8.94  LDD1902 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 9.25  LDD1902 
Metaxin-1 (MTX1) LDTP05900 17.03  LDD1902 
MFS-type transporter SLC18B1 (SLC18B1) LDTP07462 13.09  LDD1902 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 14.72  LDD1902 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 12.64  LDD1902 
Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10) LDTP04956 8.82  LDD1902 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 16.68  LDD1902 
Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) LDTP14099 13.36  LDD1902 
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) LDTP00477 7.21  LDD1902 
Mitochondrial inner membrane protein OXA1L (OXA1L) LDTP06308 9.45  LDD1902 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 7.73  LDD1902 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 9.45  LDD1902 
Mpv17-like protein 2 (MPV17L2) LDTP14952 16.34  LDD1902 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 11.16  LDD1902 
Nucleoporin NDC1 (NDC1) LDTP11213 9.13  LDD1902 
PAT complex subunit CCDC47 (CCDC47) LDTP10059 7.57  LDD1902 
Peroxisomal biogenesis factor 19 (PEX19) LDTP03905 7.31  LDD1902 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 12.47  LDD1902 
Peroxisomal membrane protein PEX16 (PEX16) LDTP14181 10.34  LDD1902 
Prosaposin (PSAP) LDTP02215 15.67  LDD1902 
Protein GPR108 (GPR108) LDTP12383 22.16  LDD1902 
Protein sel-1 homolog 1 (SEL1L) LDTP13164 9.71  LDD1902 
Protein tweety homolog 3 (TTYH3) LDTP11615 15.03  LDD1902 
Protein wntless homolog (WLS) LDTP07156 24.08  LDD1902 
Proton-coupled amino acid transporter 1 (SLC36A1) LDTP08016 7.84  LDD1902 
Putative divalent cation/proton antiporter TMEM165 (TMEM165) LDTP12207 18.00  LDD1902 
Reduced folate transporter (SLC19A1) LDTP03952 7.11  LDD1902 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 15.03  LDD1902 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 12.38  LDD1902 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 28.05  LDD1902 
Sodium-coupled neutral amino acid symporter 2 (SLC38A2) LDTP10731 10.41  LDD1902 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 13.09  LDD1902 
Solute carrier family 25 member 33 (SLC25A33) LDTP11162 10.41  LDD1902 
Solute carrier family 35 member F1 (SLC35F1) LDTP15000 19.97  LDD1902 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 13.09  LDD1902 
Solute carrier family 41 member 3 (SLC41A3) LDTP10370 7.31  LDD1902 
Sorting and assembly machinery component 50 homolog (SAMM50) LDTP14084 7.26  LDD1902 
StAR-related lipid transfer protein 3 (STARD3) LDTP06221 12.73  LDD1902 
Surfeit locus protein 4 (SURF4) LDTP00592 17.75  LDD1902 
Synaptojanin-2-binding protein (SYNJ2BP) LDTP04789 7.78  LDD1902 
Tetraspanin-3 (TSPAN3) LDTP16813 58.89  LDD1902 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 15.89  LDD1902 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 17.15  LDD1902 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 12.13  LDD1902 
Transmembrane protein 126A (TMEM126A) LDTP15948 17.27  LDD1902 
Transmembrane protein 160 (TMEM160) LDTP20002 11.08  LDD1902 
Transmembrane protein 199 (TMEM199) LDTP08881 14.42  LDD1902 
Transmembrane protein 205 (TMEM205) LDTP07667 9.45  LDD1902 
Transmembrane protein 87A (TMEM87A) LDTP15481 17.75  LDD1902 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 17.51  LDD1902 
Vang-like protein 1 (VANGL1) LDTP09288 7.67  LDD1902 
Vezatin (VEZT) LDTP12191 7.31  LDD1902 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 22.63  LDD1902 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 31.78  LDD1902 
------------------------------------------------------------------------------------
⏷ Show the Full List of 71 Transporter and channel
1 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
eIF5-mimic protein 1 (BZW2) LDTP14241 7.21  LDD1902 
------------------------------------------------------------------------------------
51 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Alpha-internexin (INA) LDTP06527 7.01  LDD1902 
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 10.34  LDD1902 
Apolipoprotein L2 (APOL2) LDTP11076 62.25  LDD1902 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 12.30  LDD1902 
Calpain small subunit 1 (CAPNS1) LDTP02047 9.58  LDD1902 
Centrosomal protein 43 (CEP43) LDTP01652 13.36  LDD1902 
Clustered mitochondria protein homolog (CLUH) LDTP14486 17.15  LDD1902 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 11.55  LDD1902 
Dynactin subunit 3 (DCTN3) LDTP01359 9.06  LDD1902 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 24.08  LDD1902 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 32.22  LDD1902 
ER lumen protein-retaining receptor 3 (KDELR3) LDTP00850 22.63  LDD1902 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 9.58  LDD1902 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 14.22  LDD1902 
Golgi phosphoprotein 3 (GOLPH3) LDTP11911 9.51  LDD1902 
Guanine nucleotide-binding protein G(i) subunit alpha-1 (GNAI1) LDTP05041 9.85  LDD1902 
Keratinocyte-associated transmembrane protein 2 (KCT2) LDTP19780 21.41  LDD1902 
Large proline-rich protein BAG6 (BAG6) LDTP04094 14.93  LDD1902 
Large ribosomal subunit protein mL55 (MRPL55) LDTP15269 9.06  LDD1902 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 23.92  LDD1902 
Membralin (TMEM259) LDTP14939 12.13  LDD1902 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 19.16  LDD1902 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 13.09  LDD1902 
NLR family member X1 (NLRX1) LDTP08248 8.40  LDD1902 
Nucleobindin-2 (NUCB2) LDTP05160 11.47  LDD1902 
Nucleosome assembly protein 1-like 1 (NAP1L1) LDTP04684 7.62  LDD1902 
Optic atrophy 3 protein (OPA3) LDTP16006 12.30  LDD1902 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 11.47  LDD1902 
Polyadenylate-binding protein-interacting protein 2 (PAIP2) LDTP11044 11.08  LDD1902 
Pre-mRNA 3'-end-processing factor FIP1 (FIP1L1) LDTP07655 8.63  LDD1902 
Protein ecdysoneless homolog (ECD) LDTP01711 7.62  LDD1902 
Protein FAM114A2 (FAM114A2) LDTP16080 11.31  LDD1902 
Protein FAM136A (FAM136A) LDTP18132 7.21  LDD1902 
Protein FAM162A (FAM162A) LDTP10056 36.50  LDD1902 
Protein LYRIC (MTDH) LDTP08224 10.13  LDD1902 
Protein SET (SET) LDTP05277 11.63  LDD1902 
Protein unc-119 homolog A (UNC119) LDTP05868 10.56  LDD1902 
Protein unc-119 homolog B (UNC119B) LDTP00105 7.11  LDD1902 
Protein YIF1A (YIF1A) LDTP01494 16.56  LDD1902 
Rab9 effector protein with kelch motifs (RABEPK) LDTP08130 7.16  LDD1902 
Reticulocalbin-2 (RCN2) LDTP06097 7.16  LDD1902 
Reticulon-3 (RTN3) LDTP01522 16.56  LDD1902 
Reticulon-4 (RTN4) LDTP12412 9.13  LDD1902 
Striatin (STRN) LDTP00875 10.48  LDD1902 
Syntaxin-18 (STX18) LDTP13086 8.51  LDD1902 
Tetraspanin-6 (TSPAN6) LDTP14458 22.47  LDD1902 
Tetratricopeptide repeat protein 38 (TTC38) LDTP19372 11.39  LDD1902 
Thioredoxin-related transmembrane protein 4 (TMX4) LDTP18373 7.67  LDD1902 
TraB domain-containing protein (TRABD) LDTP19963 7.94  LDD1902 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 8.51  LDD1902 
YEATS domain-containing protein 4 (YEATS4) LDTP01638 11.31  LDD1902 
------------------------------------------------------------------------------------
⏷ Show the Full List of 51 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1902 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587