Details of the Target

General Information of Target

Target ID LDTP09261
Target Name Major facilitator superfamily domain-containing protein 8 (MFSD8)
Gene Name MFSD8
Gene ID 256471
Synonyms
CLN7; Major facilitator superfamily domain-containing protein 8; Ceroid-lipofuscinosis neuronal protein 7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGLRNESEQEPLLGDTPGSREWDILETEEHYKSRWRSIRILYLTMFLSSVGFSVVMMSI
WPYLQKIDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCL
YAYLHIPASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGF
ILGPVFQTCFTFLGEKGVTWDVIKLQINMYTTPVLLSAFLGILNIILILAILREHRVDDS
GRQCKSINFEEASTDEAQVPQGNIDQVAVVAINVLFFVTLFIFALFETIITPLTMDMYAW
TQEQAVLYNGIILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVGFFILLPWGNQ
FPKIQWEDLHNNSIPNTTFGEIIIGLWKSPMEDDNERPTGCSIEQAWCLYTPVIHLAQFL
TSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWG
PRWAFSLVCGIIVLTITLLGVVYKRLIALSVRYGRIQE
Target Bioclass
Transporter and channel
Family
Major facilitator superfamily
Subcellular location
Lysosome membrane
Function
Outward-rectifying chloride channel involved in endolysosomal chloride homeostasis, membrane fusion and function. Conducts chloride currents up to hundreds of picoamperes. Regulates lysosomal calcium content by reducing the lysosomal membrane potential, thereby activating TRPML1 channel and further release of lysosomal calcium ions. Regulates the pH in endolysosomal compartments and may contribute to progressive acidification from endosome to lysosome. Permeable to other halides such as iodide and fluoride ions.
Uniprot ID
Q8NHS3
Ensemble ID
ENST00000296468.8
HGNC ID
HGNC:28486

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.S422P .
CHL1 SNV: p.Q10R .
COLO678 SNV: p.Q10R .
HCT15 SNV: p.G179S .
JURKAT SNV: p.V274I .
KMS27 SNV: p.I228V .
OVTOKO SNV: p.Q243P .
RKO Insertion: p.V278CfsTer10 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AOyne
 Probe Info  		11.60  LDD0443  [1]
PAL-AfBPP Probe
Click To Hide/Show 24 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C145
 Probe Info  		11.08  LDD1827  [2]
C170
 Probe Info  		15.03  LDD1850  [2]
C176
 Probe Info  		5.13  LDD1855  [2]
C177
 Probe Info  		14.12  LDD1856  [2]
C178
 Probe Info  		21.56  LDD1857  [2]
C229
 Probe Info  		20.11  LDD1902  [2]
C231
 Probe Info  		20.82  LDD1904  [2]
C232
 Probe Info  		43.41  LDD1905  [2]
C233
 Probe Info  		21.71  LDD1906  [2]
C234
 Probe Info  		7.89  LDD1907  [2]
C235
 Probe Info  		29.65  LDD1908  [2]
C265
 Probe Info  		12.82  LDD1936  [2]
C270
 Probe Info  		8.51  LDD1940  [2]
C273
 Probe Info  		5.21  LDD1943  [2]
C277
 Probe Info  		7.73  LDD1947  [2]
C303
 Probe Info  		4.92  LDD1972  [2]
C307
 Probe Info  		8.69  LDD1975  [2]
C310
 Probe Info  		9.58  LDD1977  [2]
C424
 Probe Info  		9.51  LDD2079  [2]
C426
 Probe Info  		31.78  LDD2081  [2]
C428
 Probe Info  		8.40  LDD2083  [2]
FFF probe11
 Probe Info  		20.00  LDD0471  [3]
FFF probe13
 Probe Info  		20.00  LDD0475  [3]
OEA-DA
 Probe Info  		20.00  LDD0046  [4]

References

1 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
2 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
3 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
4 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570