General Information of Target

Target ID LDTP08878
Target Name ER membrane protein complex subunit 5 (MMGT1)
Gene Name MMGT1
Gene ID 93380
Synonyms
EMC5; TMEM32; ER membrane protein complex subunit 5; Membrane magnesium transporter 1; Transmembrane protein 32
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPSLWKGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDESLPIDIVLQTLLAFAVTCYGI
VHIAGEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVLFRPSDTANSSNQDALSSNTS
LKLRKLESLRR
Target Bioclass
Transporter and channel
Family
Membrane magnesium transporter (TC 1.A.67) family
Subcellular location
Endoplasmic reticulum membrane
Function
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes. May be involved in Mg(2+) transport.
Uniprot ID
Q8N4V1
Ensemble ID
ENST00000305963.3
HGNC ID
HGNC:28100

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.S40T .
WM2664 Insertion: p.E66RfsTer3 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info 
2.18  LDD0197  [1]
STPyne
 Probe Info 
K79(9.09)  LDD0277  [2]
SF
 Probe Info 
N.A.  LDD0028  [3]
Ox-W18
 Probe Info 
N.A.  LDD2175  [4]
1c-yne
 Probe Info 
N.A.  LDD0228  [5]
Acrolein
 Probe Info 
N.A.  LDD0217  [6]
NAIA_5
 Probe Info 
N.A.  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 93 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info 
23.43  LDD1740  [8]
C041
 Probe Info 
6.15  LDD1741  [8]
C049
 Probe Info 
22.32  LDD1747  [8]
C053
 Probe Info 
8.40  LDD1751  [8]
C055
 Probe Info 
29.65  LDD1752  [8]
C056
 Probe Info 
79.89  LDD1753  [8]
C063
 Probe Info 
46.21  LDD1760  [8]
C067
 Probe Info 
18.77  LDD1763  [8]
C070
 Probe Info 
55.33  LDD1766  [8]
C071
 Probe Info 
8.11  LDD1767  [8]
C072
 Probe Info 
27.67  LDD1768  [8]
C091
 Probe Info 
54.19  LDD1782  [8]
C092
 Probe Info 
58.89  LDD1783  [8]
C094
 Probe Info 
85.04  LDD1785  [8]
C095
 Probe Info 
10.06  LDD1786  [8]
C100
 Probe Info 
21.56  LDD1789  [8]
C106
 Probe Info 
30.27  LDD1793  [8]
C107
 Probe Info 
41.93  LDD1794  [8]
C108
 Probe Info 
9.85  LDD1795  [8]
C112
 Probe Info 
48.17  LDD1799  [8]
C129
 Probe Info 
5.90  LDD1811  [8]
C134
 Probe Info 
99.73  LDD1816  [8]
C135
 Probe Info 
32.45  LDD1817  [8]
C138
 Probe Info 
5.54  LDD1820  [8]
C139
 Probe Info 
25.63  LDD1821  [8]
C140
 Probe Info 
8.94  LDD1822  [8]
C141
 Probe Info 
10.63  LDD1823  [8]
C143
 Probe Info 
20.39  LDD1825  [8]
C158
 Probe Info 
24.08  LDD1838  [8]
C159
 Probe Info 
19.70  LDD1839  [8]
C160
 Probe Info 
12.21  LDD1840  [8]
C161
 Probe Info 
67.18  LDD1841  [8]
C169
 Probe Info 
99.04  LDD1849  [8]
C172
 Probe Info 
6.06  LDD1852  [8]
C183
 Probe Info 
11.08  LDD1861  [8]
C185
 Probe Info 
8.63  LDD1863  [8]
C186
 Probe Info 
33.13  LDD1864  [8]
C194
 Probe Info 
14.03  LDD1870  [8]
C198
 Probe Info 
17.27  LDD1874  [8]
C199
 Probe Info 
8.22  LDD1875  [8]
C229
 Probe Info 
18.90  LDD1902  [8]
C231
 Probe Info 
99.73  LDD1904  [8]
C232
 Probe Info 
99.73  LDD1905  [8]
C233
 Probe Info 
22.94  LDD1906  [8]
C234
 Probe Info 
11.24  LDD1907  [8]
C235
 Probe Info 
71.51  LDD1908  [8]
C238
 Probe Info 
36.50  LDD1911  [8]
C240
 Probe Info 
17.88  LDD1913  [8]
C243
 Probe Info 
10.93  LDD1916  [8]
C246
 Probe Info 
24.76  LDD1919  [8]
C249
 Probe Info 
17.63  LDD1922  [8]
C252
 Probe Info 
13.64  LDD1925  [8]
C278
 Probe Info 
99.73  LDD1948  [8]
C282
 Probe Info 
18.64  LDD1952  [8]
C285
 Probe Info 
24.59  LDD1955  [8]
C287
 Probe Info 
14.52  LDD1957  [8]
C288
 Probe Info 
19.84  LDD1958  [8]
C289
 Probe Info 
99.73  LDD1959  [8]
C293
 Probe Info 
20.68  LDD1963  [8]
C296
 Probe Info 
19.29  LDD1966  [8]
C310
 Probe Info 
10.63  LDD1977  [8]
C322
 Probe Info 
10.06  LDD1988  [8]
C326
 Probe Info 
17.15  LDD1990  [8]
C338
 Probe Info 
46.85  LDD2001  [8]
C339
 Probe Info 
17.03  LDD2002  [8]
C343
 Probe Info 
15.89  LDD2005  [8]
C348
 Probe Info 
30.48  LDD2009  [8]
C349
 Probe Info 
22.32  LDD2010  [8]
C350
 Probe Info 
67.18  LDD2011  [8]
C355
 Probe Info 
56.49  LDD2016  [8]
C356
 Probe Info 
19.43  LDD2017  [8]
C357
 Probe Info 
6.36  LDD2018  [8]
C362
 Probe Info 
99.73  LDD2023  [8]
C363
 Probe Info 
66.72  LDD2024  [8]
C364
 Probe Info 
66.26  LDD2025  [8]
C366
 Probe Info 
21.71  LDD2027  [8]
C378
 Probe Info 
7.06  LDD2037  [8]
C380
 Probe Info 
5.39  LDD2039  [8]
C382
 Probe Info 
22.78  LDD2041  [8]
C386
 Probe Info 
6.82  LDD2045  [8]
C388
 Probe Info 
99.73  LDD2047  [8]
C390
 Probe Info 
99.73  LDD2049  [8]
C391
 Probe Info 
17.27  LDD2050  [8]
C399
 Probe Info 
21.86  LDD2058  [8]
C403
 Probe Info 
99.73  LDD2061  [8]
C405
 Probe Info 
8.57  LDD2063  [8]
C407
 Probe Info 
49.87  LDD2064  [8]
C413
 Probe Info 
47.50  LDD2069  [8]
C419
 Probe Info 
50.21  LDD2074  [8]
C420
 Probe Info 
18.77  LDD2075  [8]
FFF probe13
 Probe Info 
20.00  LDD0475  [9]
FFF probe2
 Probe Info 
20.00  LDD0463  [9]
OEA-DA
 Probe Info 
6.29  LDD0046  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Anterior gradient protein 3 (AGR3) AGR family Q8TD06
NADH dehydrogenase 1 alpha subcomplex subunit 3 (NDUFA3) Complex I NDUFA3 subunit family O95167
Polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6 (PLPP6) PA-phosphatase related phosphoesterase family Q8IY26
Serine/threonine-protein kinase VRK2 (VRK2) CK1 Ser/Thr protein kinase family Q86Y07
Very-long-chain enoyl-CoA reductase (TECR) Steroid 5-alpha reductase family Q9NZ01
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
Transporter and channel
Click To Hide/Show 20 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Solute carrier family 7 member 14 (SLC7A14) Cationic amino acid transporter (CAT) (TC 2.A.3.3) family Q8TBB6
Sodium-coupled neutral amino acid transporter 7 (SLC38A7) Amino acid/polyamine transporter 2 family Q9NVC3
Bax inhibitor 1 (TMBIM6) BI1 family P55061
Complement component C8 alpha chain (C8A) Complement C6/C7/C8/C9 family P07357
ER membrane protein complex subunit 2 (EMC2) EMC2 family Q15006
ER membrane protein complex subunit 6 (EMC6) EMC6 family Q9BV81
FXYD domain-containing ion transport regulator 6 (FXYD6) FXYD family Q9H0Q3
Insulin-induced gene 2 protein (INSIG2) INSIG family Q9Y5U4
Major facilitator superfamily domain-containing protein 3 (MFSD3) Major facilitator superfamily Q96ES6
Molybdate-anion transporter (MFSD5) Major facilitator superfamily Q6N075
Membrane-spanning 4-domains subfamily A member 13 (MS4A13) MS4A family Q5J8X5
Nucleotide sugar transporter SLC35B4 (SLC35B4) Nucleotide-sugar transporter family Q969S0
Peroxisomal membrane protein PEX16 (PEX16) Peroxin-16 family Q9Y5Y5
Syntaxin-1A (STX1A) Syntaxin family Q16623
Syntaxin-3 (STX3) Syntaxin family Q13277
Syntaxin-6 (STX6) Syntaxin family O43752
Transmembrane protein 14A (TMEM14A) TMEM14 family Q9Y6G1
Transmembrane protein 14C (TMEM14C) TMEM14 family Q9P0S9
Solute carrier family 35 member E4 (SLC35E4) TPT transporter family Q6ICL7
Transmembrane protein 60 (TMEM60) . Q9H2L4
GPCR
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable G-protein coupled receptor 25 (GPR25) G-protein coupled receptor 1 family O00155
Adhesion G protein-coupled receptor E2 (ADGRE2) G-protein coupled receptor 2 family Q9UHX3
Metabotropic glutamate receptor 2 (GRM2) G-protein coupled receptor 3 family Q14416
Taste receptor type 2 member 19 (TAS2R19) G-protein coupled receptor T2R family P59542
Other
Click To Hide/Show 19 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Beta-defensin 127 (DEFB127) Beta-defensin family Q9H1M4
Mitochondrial fission 1 protein (FIS1) FIS1 family Q9Y3D6
Golgi SNAP receptor complex member 2 (GOSR2) GOSR2 family O14653
Integrin alpha-M (ITGAM) Integrin alpha chain family P11215
Ninjurin-2 (NINJ2) Ninjurin family Q9NZG7
ORM1-like protein 2 (ORMDL2) ORM family Q53FV1
RUS family member 1 (RUSF1) RUS1 family Q96GQ5
Vesicle-trafficking protein SEC22b (SEC22B) Synaptobrevin family O75396
Syntaxin-12 (STX12) Syntaxin family Q86Y82
Syntaxin-1B (STX1B) Syntaxin family P61266
Syntaxin-8 (STX8) Syntaxin family Q9UNK0
Transmembrane protein 229B (TMEM229B) TMEM229 family Q8NBD8
Protein YIF1A (YIF1A) YIF1 family O95070
Complement C5 (C5) . P01031
Emerin (EMD) . P50402
Protein TMED8 (TMED8) . Q6PL24
Thrombomodulin (THBD) . P07204
Transmembrane protein 254 (TMEM254) . Q8TBM7
Transmembrane protein 42 (TMEM42) . Q69YG0

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
4 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
5 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
10 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570