Details of the Probe

General Information of Probe

Probe ID
LDPC0597
Probe Name
C378
Synonyms
Z4225967456
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 282.3
Lipid-water partition coefficient (xlogp) 1.2
Rotatable Bond Count (rotbonds) 6
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 5
Chemical Identifiers
Formula
C14H14N6O
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]pyrrolo[2,1-f][1,2,4]triazine-4-carboxamide
Canonical SMILES
C#CCCC1(N=N1)CCNC(=O)C2=NC=NN3C2=CC=C3
InChI
InChI=1S/C14H14N6O/c1-2-3-6-14(18-19-14)7-8-15-13(21)12-11-5-4-9-20(11)17-10-16-12/h1,4-5,9-10H,3,6-8H2,(H,15,21)
InChIKey
URZNVGPLOLOWPJ-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

75 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) LDTP06325 17.27  LDD2037 
5'-3' exonuclease PLD3 (PLD3) LDTP08476 7.01  LDD2037 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 10.85  LDD2037 
Acid ceramidase (ASAH1) LDTP05905 23.10  LDD2037 
Adenosine deaminase (ADA) LDTP01803 14.52  LDD2037 
ADP-dependent glucokinase (ADPGK) LDTP11127 6.50  LDD2037 
Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) LDTP04437 6.92  LDD2037 
Aldehyde dehydrogenase, mitochondrial (ALDH2) LDTP02074 6.54  LDD2037 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 6.59  LDD2037 
Apoptosis-inducing factor 1, mitochondrial (AIFM1) LDTP01687 8.22  LDD2037 
ATP-dependent translocase ABCB1 (ABCB1) LDTP02254 6.73  LDD2037 
Beta-galactosidase (GLB1) LDTP02837 14.93  LDD2037 
Beta-hexosaminidase subunit alpha (HEXA) LDTP02169 7.01  LDD2037 
Beta-hexosaminidase subunit beta (HEXB) LDTP02216 9.00  LDD2037 
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 7.26  LDD2037 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 8.28  LDD2037 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 8.22  LDD2037 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 6.68  LDD2037 
Ceramide synthase 2 (CERS2) LDTP10329 10.27  LDD2037 
Choline kinase alpha (CHKA) LDTP03787 7.84  LDD2037 
Cytochrome b-c1 complex subunit 8 (UQCRQ) LDTP00486 8.94  LDD2037 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 9.13  LDD2037 
Cytochrome b5 type B (CYB5B) LDTP00697 11.08  LDD2037 
DCN1-like protein 4 (DCUN1D4) LDTP09799 6.77  LDD2037 
Dehydrogenase/reductase SDR family member 1 (DHRS1) LDTP10550 6.54  LDD2037 
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) LDTP05724 10.20  LDD2037 
E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) LDTP10699 8.40  LDD2037 
Endonuclease domain-containing 1 protein (ENDOD1) LDTP01462 7.06  LDD2037 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 8.57  LDD2037 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 8.46  LDD2037 
Epoxide hydrolase 1 (EPHX1) LDTP02175 10.93  LDD2037 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 8.69  LDD2037 
Farnesyl pyrophosphate synthase (FDPS) LDTP02721 6.54  LDD2037 
Ferrochelatase, mitochondrial (FECH) LDTP03198 15.03  LDD2037 
Heme oxygenase 1 (HMOX1) LDTP02359 15.56  LDD2037 
Heme oxygenase 2 (HMOX2) LDTP03540 14.62  LDD2037 
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (PDE8A) LDTP01018 5.86  LDD2037 
Histone-lysine N-methyltransferase SMYD3 (SMYD3) LDTP12020 6.06  LDD2037 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 6.45  LDD2037 
L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) LDTP12105 5.90  LDD2037 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 9.51  LDD2037 
Leucyl-cystinyl aminopeptidase (LNPEP) LDTP13358 6.06  LDD2037 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 28.84  LDD2037 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 10.13  LDD2037 
Lysosomal protective protein (CTSA) LDTP02505 6.02  LDD2037 
Macrophage migration inhibitory factor (MIF) LDTP02713 7.89  LDD2037 
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) LDTP11273 6.28  LDD2037 
NAD(P) transhydrogenase, mitochondrial (NNT) LDTP05862 7.01  LDD2037 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 8.63  LDD2037 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 10.85  LDD2037 
Nicotinamide phosphoribosyltransferase (NAMPT) LDTP04051 8.82  LDD2037 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 11.88  LDD2037 
Peroxisomal acyl-coenzyme A oxidase 3 (ACOX3) LDTP00589 7.62  LDD2037 
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma (PIP4K2C) LDTP09355 6.41  LDD2037 
Phospholipase A2 group XV (PLA2G15) LDTP09088 7.31  LDD2037 
Pirin (PIR) LDTP00325 6.54  LDD2037 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 12.55  LDD2037 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 11.71  LDD2037 
Prostaglandin reductase 3 (PTGR3) LDTP15422 5.86  LDD2037 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 6.28  LDD2037 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 8.06  LDD2037 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 8.69  LDD2037 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 16.45  LDD2037 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 10.34  LDD2037 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 8.40  LDD2037 
RNA cytidine acetyltransferase (NAT10) LDTP11690 7.89  LDD2037 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 7.84  LDD2037 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 8.94  LDD2037 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 6.06  LDD2037 
Sphingosine-1-phosphate lyase 1 (SGPL1) LDTP01613 6.06  LDD2037 
Squalene monooxygenase (SQLE) LDTP06142 6.02  LDD2037 
Sulfotransferase 2A1 (SULT2A1) LDTP05493 7.41  LDD2037 
Transmembrane protein with metallophosphoesterase domain (TMPPE) LDTP07831 7.41  LDD2037 
Trimethyllysine dioxygenase, mitochondrial (TMLHE) LDTP12679 6.32  LDD2037 
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) LDTP08728 10.63  LDD2037 
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⏷ Show the Full List of 75 Enzyme
48 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) LDTP09328 8.34  LDD2037 
ATP synthase subunit a (MT-ATP6) LDTP01804 8.63  LDD2037 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 6.19  LDD2037 
Cytochrome c oxidase subunit 5A, mitochondrial (COX5A) LDTP03064 11.79  LDD2037 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 6.02  LDD2037 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 31.12  LDD2037 
ER membrane protein complex subunit 5 (MMGT1) LDTP08878 7.06  LDD2037 
H(+)/Cl(-) exchange transporter 7 (CLCN7) LDTP04463 7.01  LDD2037 
Lysosome membrane protein 2 (SCARB2) LDTP06034 13.27  LDD2037 
Lysosome-associated membrane glycoprotein 2 (LAMP2) LDTP02655 6.63  LDD2037 
Magnesium transporter NIPA3 (NIPAL1) LDTP17362 9.92  LDD2037 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 9.58  LDD2037 
Metaxin-1 (MTX1) LDTP05900 6.19  LDD2037 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 6.28  LDD2037 
Mitochondrial chaperone BCS1 (BCS1L) LDTP13823 6.06  LDD2037 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 8.06  LDD2037 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 19.70  LDD2037 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 26.54  LDD2037 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 6.11  LDD2037 
Mpv17-like protein 2 (MPV17L2) LDTP14952 6.02  LDD2037 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 7.26  LDD2037 
Nucleoporin NDC1 (NDC1) LDTP11213 6.68  LDD2037 
Prosaposin (PSAP) LDTP02215 10.27  LDD2037 
Protein FAM8A1 (FAM8A1) LDTP16190 7.57  LDD2037 
Protein GPR107 (GPR107) LDTP07236 7.16  LDD2037 
Protein GPR108 (GPR108) LDTP12383 9.51  LDD2037 
Protein spinster homolog 1 (SPNS1) LDTP11844 7.46  LDD2037 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 7.16  LDD2037 
Secretory carrier-associated membrane protein 2 (SCAMP2) LDTP00547 10.93  LDD2037 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 7.41  LDD2037 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 7.16  LDD2037 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 6.54  LDD2037 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 5.90  LDD2037 
StAR-related lipid transfer protein 3 (STARD3) LDTP06221 12.91  LDD2037 
STARD3 N-terminal-like protein (STARD3NL) LDTP01671 6.63  LDD2037 
Surfeit locus protein 4 (SURF4) LDTP00592 12.64  LDD2037 
Tetraspanin-10 (TSPAN10) LDTP15967 7.94  LDD2037 
Tetraspanin-3 (TSPAN3) LDTP16813 14.72  LDD2037 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 12.55  LDD2037 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 6.59  LDD2037 
Transmembrane emp24 domain-containing protein 2 (TMED2) LDTP06365 8.57  LDD2037 
Transmembrane protein 126A (TMEM126A) LDTP15948 15.78  LDD2037 
Transmembrane protein 160 (TMEM160) LDTP20002 14.72  LDD2037 
Transmembrane protein 199 (TMEM199) LDTP08881 9.71  LDD2037 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 6.15  LDD2037 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 26.54  LDD2037 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 20.25  LDD2037 
Zinc transporter SLC39A7 (SLC39A7) LDTP09769 6.28  LDD2037 
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⏷ Show the Full List of 48 Transporter and channel
39 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 9.78  LDD2037 
Apolipoprotein L2 (APOL2) LDTP11076 45.89  LDD2037 
Centrosomal protein 43 (CEP43) LDTP01652 5.98  LDD2037 
Clustered mitochondria protein homolog (CLUH) LDTP14486 8.46  LDD2037 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 12.30  LDD2037 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 6.02  LDD2037 
Ganglioside GM2 activator (GM2A) LDTP02927 5.94  LDD2037 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 6.50  LDD2037 
Kinetochore protein Spc24 (SPC24) LDTP09069 8.46  LDD2037 
Large proline-rich protein BAG6 (BAG6) LDTP04094 13.09  LDD2037 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 15.35  LDD2037 
Microtubule nucleation factor SSNA1 (SSNA1) LDTP00871 12.91  LDD2037 
Mitochondrial import receptor subunit TOM20 homolog (TOMM20) LDTP06373 6.59  LDD2037 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 25.28  LDD2037 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 8.94  LDD2037 
Neuferricin (CYB5D2) LDTP15623 5.86  LDD2037 
Nucleobindin-2 (NUCB2) LDTP05160 11.79  LDD2037 
Nurim (NRM) LDTP08606 5.90  LDD2037 
Optic atrophy 3 protein (OPA3) LDTP16006 10.06  LDD2037 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 6.28  LDD2037 
Polycomb protein EED (EED) LDTP01249 12.82  LDD2037 
Protein CEBPZOS (CEBPZOS) LDTP18883 9.51  LDD2037 
Protein CutA (CUTA) LDTP01075 6.06  LDD2037 
Protein FAM114A2 (FAM114A2) LDTP16080 7.36  LDD2037 
Protein unc-119 homolog A (UNC119) LDTP05868 8.57  LDD2037 
Protein unc-119 homolog B (UNC119B) LDTP00105 10.70  LDD2037 
Protein XRP2 (RP2) LDTP01296 6.50  LDD2037 
Reticulon-2 (RTN2) LDTP01167 5.94  LDD2037 
Reticulon-3 (RTN3) LDTP01522 6.59  LDD2037 
Reticulon-4 (RTN4) LDTP12412 6.36  LDD2037 
Signal recognition particle receptor subunit beta (SRPRB) LDTP16323 7.94  LDD2037 
Syntaxin-18 (STX18) LDTP13086 7.89  LDD2037 
Tetraspanin-6 (TSPAN6) LDTP14458 13.55  LDD2037 
Transmembrane protein 209 (TMEM209) LDTP18272 5.86  LDD2037 
Tubulin beta chain (TUBB) LDTP02205 6.82  LDD2037 
Tubulin beta-2B chain (TUBB2B) LDTP11277 5.94  LDD2037 
Tubulin beta-4B chain (TUBB4B) LDTP05076 6.45  LDD2037 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 20.11  LDD2037 
Zinc finger CCCH domain-containing protein 7B (ZC3H7B) LDTP13245 10.27  LDD2037 
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⏷ Show the Full List of 39 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD2037 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587