Details of the Target

General Information of Target

Target ID LDTP06325
Target Name 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP)
Gene Name EBP
Gene ID 10682
Synonyms
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase; EC 5.3.3.5; Cholestenol Delta-isomerase; Delta(8)-Delta(7) sterol isomerase; D8-D7 sterol isomerase; Emopamil-binding protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTTNAGPLHPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVTTWLLSGRAAVVPLGT
WRRLSLCWFAVCGFIHLVIEGWFVLYYEDLLGDQAFLSQLWKEYAKGDSRYILGDNFTVC
METITACLWGPLSLWVVIAFLRQHPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGELG
HPLYFWFYFVFMNALWLVLPGVLVLDAVKHLTHAQSTLDAKATKAKSKKN
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
EBP family
Subcellular location
Endoplasmic reticulum membrane
Function Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers.
TTD ID
T25317
Uniprot ID
Q15125
DrugMap ID
TT4VQZX
Ensemble ID
ENST00000495186.6
HGNC ID
HGNC:3133
ChEMBL ID
CHEMBL4931

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		8.01  LDD0066  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
YN-4
 Probe Info  		100.00  LDD0445  [2]
ONAyne
 Probe Info  		K106(7.94)  LDD0275  [3]
OPA-S-S-alkyne
 Probe Info  		K106(4.19)  LDD3494  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
ATP probe
 Probe Info  		K221(0.00); K224(0.00)  LDD0199  [6]
Acrolein
 Probe Info  		H210(0.00); H213(0.00)  LDD0217  [7]
Crotonaldehyde
 Probe Info  		H213(0.00); H210(0.00)  LDD0219  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 46 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		13.93  LDD1728  [9]
C039
 Probe Info  		5.90  LDD1739  [9]
C040
 Probe Info  		38.05  LDD1740  [9]
C041
 Probe Info  		32.90  LDD1741  [9]
C049
 Probe Info  		5.98  LDD1747  [9]
C063
 Probe Info  		21.11  LDD1760  [9]
C064
 Probe Info  		16.34  LDD1761  [9]
C082
 Probe Info  		5.28  LDD1774  [9]
C085
 Probe Info  		18.00  LDD1777  [9]
C100
 Probe Info  		6.06  LDD1789  [9]
C106
 Probe Info  		19.29  LDD1793  [9]
C107
 Probe Info  		41.93  LDD1794  [9]
C108
 Probe Info  		10.06  LDD1795  [9]
C112
 Probe Info  		21.56  LDD1799  [9]
C134
 Probe Info  		38.32  LDD1816  [9]
C163
 Probe Info  		12.73  LDD1843  [9]
C164
 Probe Info  		6.68  LDD1844  [9]
C165
 Probe Info  		19.03  LDD1845  [9]
C166
 Probe Info  		9.99  LDD1846  [9]
C169
 Probe Info  		25.63  LDD1849  [9]
C183
 Probe Info  		8.28  LDD1861  [9]
C185
 Probe Info  		6.92  LDD1863  [9]
C186
 Probe Info  		11.71  LDD1864  [9]
C201
 Probe Info  		33.82  LDD1877  [9]
C287
 Probe Info  		12.82  LDD1957  [9]
C288
 Probe Info  		42.22  LDD1958  [9]
C290
 Probe Info  		4.96  LDD1960  [9]
C296
 Probe Info  		72.50  LDD1966  [9]
C314
 Probe Info  		62.68  LDD1981  [9]
C322
 Probe Info  		9.13  LDD1988  [9]
C326
 Probe Info  		6.82  LDD1990  [9]
C343
 Probe Info  		12.73  LDD2005  [9]
C355
 Probe Info  		43.11  LDD2016  [9]
C356
 Probe Info  		9.78  LDD2017  [9]
C376
 Probe Info  		16.56  LDD2036  [9]
C378
 Probe Info  		17.27  LDD2037  [9]
C379
 Probe Info  		5.98  LDD2038  [9]
C380
 Probe Info  		17.88  LDD2039  [9]
C382
 Probe Info  		18.77  LDD2041  [9]
C383
 Probe Info  		11.55  LDD2042  [9]
C386
 Probe Info  		7.46  LDD2045  [9]
C388
 Probe Info  		52.71  LDD2047  [9]
C389
 Probe Info  		5.98  LDD2048  [9]
STS-1
 Probe Info  		N.A.  LDD0136  [10]
VE-P
 Probe Info  		N.A.  LDD0396  [11]
A-DA
 Probe Info  		16.03  LDD0144  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H213(0.00); H210(0.00)  LDD0222  [7]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0107  IAA HeLa H210(0.00); H213(0.00)  LDD0221  [7]
 LDCM0109  NEM HeLa H213(0.00); H210(0.00)  LDD0223  [7]
 LDCM0084  Ro 48-8071 A-549 16.03  LDD0144  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 35 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysophosphatidylcholine acyltransferase 2 (LPCAT2) 1-acyl-sn-glycerol-3-phosphate acyltransferase family Q7L5N7
Phosphatidylserine lipase ABHD16A (ABHD16A) ABHD16 family O95870
Inactive pancreatic lipase-related protein 1 (PNLIPRP1) Lipase family P54315
N-acetyltransferase 8 (NAT8) Camello family Q9UHE5
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT) CDP-alcohol phosphatidyltransferase class-I family O14735
NADH dehydrogenase 1 alpha subcomplex subunit 3 (NDUFA3) Complex I NDUFA3 subunit family O95167
NADH dehydrogenase 1 beta subcomplex subunit 11, mitochondrial (NDUFB11) Complex I NDUFB11 subunit family Q9NX14
Cytochrome b5 type B (CYB5B) Cytochrome b5 family O43169
Cytochrome P450 4F2 (CYP4F2) Cytochrome P450 family P78329
Probable palmitoyltransferase ZDHHC24 (ZDHHC24) DHHC palmitoyltransferase family Q6UX98
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) EBP family Q15125
Peroxisomal bifunctional enzyme (EHHADH) Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family Q08426
Sialidase-1 (NEU1) Glycosyl hydrolase 33 family Q99519
Heme oxygenase 2 (HMOX2) Heme oxygenase family P30519
Leukotriene C4 synthase (LTC4S) MAPEG family Q16873
Polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6 (PLPP6) PA-phosphatase related phosphoesterase family Q8IY26
Matrix metalloproteinase-14 (MMP14) Peptidase M10A family P50281
Complement C2 (C2) Peptidase S1 family P06681
Tyrosine-protein phosphatase non-receptor type 9 (PTPN9) Protein-tyrosine phosphatase family P43378
E3 ubiquitin-protein ligase RNF152 (RNF152) RNF152 family Q8N8N0
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
ADP-ribosylation factor-like protein 13B (ARL13B) Arf family Q3SXY8
Fatty acid hydroxylase domain-containing protein 2 (FAXDC2) Sterol desaturase family Q96IV6
Fatty acid 2-hydroxylase (FA2H) Sterol desaturase family Q7L5A8
TLC domain-containing protein 4 (TLCD4) TLCD4 family Q96MV1
Lysoplasmalogenase TMEM86A (TMEM86A) TMEM86 family Q8N2M4
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
GTPase IMAP family member 1 (GIMAP1) AIG1/Toc34/Toc159-like paraseptin GTPase family Q8WWP7
GTPase IMAP family member 5 (GIMAP5) AIG1/Toc34/Toc159-like paraseptin GTPase family Q96F15
UbiA prenyltransferase domain-containing protein 1 (UBIAD1) UbiA prenyltransferase family Q9Y5Z9
Ubiquitin-conjugating enzyme E2 J1 (UBE2J1) Ubiquitin-conjugating enzyme family Q9Y385
Peptidyl-prolyl cis-trans isomerase FKBP8 (FKBP8) . Q14318
Phosphatidylinositol-3-phosphatase SAC1 (SACM1L) . Q9NTJ5
TLC domain-containing protein 1 (TLCD1) . Q96CP7
Transmembrane ascorbate-dependent reductase CYB561 (CYB561) . P49447
Transporter and channel
Click To Hide/Show 52 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sodium-coupled neutral amino acid transporter 7 (SLC38A7) Amino acid/polyamine transporter 2 family Q9NVC3
Protein ARV1 (ARV1) ARV1 family Q9H2C2
Beta-defensin 103 (DEFB103A; DEFB103B) Beta-defensin family P81534
Bax inhibitor 1 (TMBIM6) BI1 family P55061
Proton-coupled zinc antiporter SLC30A8 (SLC30A8) Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family Q8IWU4
Gap junction beta-2 protein (GJB2) Connexin family P29033
ER membrane protein complex subunit 6 (EMC6) EMC6 family Q9BV81
FXYD domain-containing ion transport regulator 6 (FXYD6) FXYD family Q9H0Q3
Glycophorin-A (GYPA) Glycophorin A family P02724
Heme transporter HRG1 (SLC48A1) HRG family Q6P1K1
Insulin-induced gene 2 protein (INSIG2) INSIG family Q9Y5U4
Major facilitator superfamily domain-containing protein 6 (MFSD6) MFSD6 family Q6ZSS7
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Membrane-spanning 4-domains subfamily A member 13 (MS4A13) MS4A family Q5J8X5
Ninjurin-1 (NINJ1) Ninjurin family Q92982
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) NIP3 family Q12983
Probable UDP-sugar transporter protein SLC35A4 (SLC35A4) Nucleotide-sugar transporter family Q96G79
Proton channel OTOP3 (OTOP3) Otopetrin family Q7RTS5
Peripheral myelin protein 22 (PMP22) PMP-22/EMP/MP20 family Q01453
Secretory carrier-associated membrane protein 4 (SCAMP4) SCAMP family Q969E2
Solute carrier family 41 member 2 (SLC41A2) SLC41A transporter family Q96JW4
Small integral membrane protein 1 (SMIM1) SMIM1 family B2RUZ4
Vesicle-associated membrane protein 2 (VAMP2) Synaptobrevin family P63027
Vesicle-associated membrane protein 3 (VAMP3) Synaptobrevin family Q15836
Syntaxin-5 (STX5) Syntaxin family Q13190
Syntaxin-6 (STX6) Syntaxin family O43752
CD81 antigen (CD81) Tetraspanin (TM4SF) family P60033
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) Tim17/Tim22/Tim23 family O14925
Ion channel TACAN (TMEM120A) TMEM120 family Q9BXJ8
Transmembrane protein 14B (TMEM14B) TMEM14 family Q9NUH8
Transmembrane protein 14C (TMEM14C) TMEM14 family Q9P0S9
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8
Solute carrier family 35 member G1 (SLC35G1) TMEM20 family Q2M3R5
Transmembrane protein 208 (TMEM208) TMEM208 family Q9BTX3
Transmembrane protein 218 (TMEM218) TMEM218 family A2RU14
Transmembrane protein 242 (TMEM242) TMEM242 family Q9NWH2
Sigma intracellular receptor 2 (TMEM97) TMEM97/sigma-2 receptor family Q5BJF2
Translocator protein 2 (TSPO2) TspO/BZRP family Q5TGU0
Protein unc-93 homolog B1 (UNC93B1) Unc-93 family Q9H1C4
Vesicle-associated membrane protein-associated protein A (VAPA) VAMP-associated protein (VAP) (TC 9.B.17) family Q9P0L0
Vesicle-associated membrane protein-associated protein B/C (VAPB) VAMP-associated protein (VAP) (TC 9.B.17) family O95292
Protein YIPF1 (YIPF1) YIP1 family Q9Y548
Early activation antigen CD69 (CD69) . Q07108
Leucine-rich repeat-containing protein 59 (LRRC59) . Q96AG4
Phospholipid transfer protein C2CD2L (C2CD2L) . O14523
Proteolipid protein 2 (PLP2) . Q04941
Synaptojanin-2-binding protein (SYNJ2BP) . P57105
Transmembrane protein 100 (TMEM100) . Q9NV29
Transmembrane protein 199 (TMEM199) . Q8N511
Transmembrane protein 203 (TMEM203) . Q969S6
Transmembrane protein 60 (TMEM60) . Q9H2L4
Transmembrane protein 65 (TMEM65) . Q6PI78
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable G-protein coupled receptor 152 (GPR152) G-protein coupled receptor 1 family Q8TDT2
Immunoglobulin
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Butyrophilin subfamily 2 member A2 (BTN2A2) BTN/MOG family Q8WVV5
Poliovirus receptor (PVR) Nectin family P15151
Cytokine and receptor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
C-C motif chemokine 4-like (CCL4L1; CCL4L2) Intercrine beta (chemokine CC) family Q8NHW4
Tumor necrosis factor receptor superfamily member 5 (CD40) . P25942
Other
Click To Hide/Show 66 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ADP-ribosylation factor-like protein 6-interacting protein 6 (ARL6IP6) ARL6IP6 family Q8N6S5
Beta-defensin 127 (DEFB127) Beta-defensin family Q9H1M4
Gap junction beta-6 protein (GJB6) Connexin family O95452
Ergosterol biosynthetic protein 28 homolog (ERG28) ERG28 family Q9UKR5
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Fetuin-B (FETUB) Fetuin family Q9UGM5
Mitochondrial fission 1 protein (FIS1) FIS1 family Q9Y3D6
FUN14 domain-containing protein 2 (FUNDC2) FUN14 family Q9BWH2
Golgi SNAP receptor complex member 2 (GOSR2) GOSR2 family O14653
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Protein kish-B (TMEM167B) KISH family Q9NRX6
Low-density lipoprotein receptor class A domain-containing protein 1 (LDLRAD1) LDLR family Q5T700
Low-density lipoprotein receptor-related protein 10 (LRP10) LDLR family Q7Z4F1
Plasmolipin (PLLP) MAL family Q9Y342
Ninjurin-2 (NINJ2) Ninjurin family Q9NZG7
Nurim (NRM) Nurim family Q8IXM6
ORM1-like protein 1 (ORMDL1) ORM family Q9P0S3
ORM1-like protein 2 (ORMDL2) ORM family Q53FV1
ORM1-like protein 3 (ORMDL3) ORM family Q8N138
Claudin domain-containing protein 2 (CLDND2) PMP-22/EMP/MP20 family Q8NHS1
Epithelial membrane protein 1 (EMP1) PMP-22/EMP/MP20 family P54849
Protein NKG7 (NKG7) PMP-22/EMP/MP20 family Q16617
Stress-associated endoplasmic reticulum protein 1 (SERP1) RAMP4 family Q9Y6X1
Stress-associated endoplasmic reticulum protein 2 (SERP2) RAMP4 family Q8N6R1
RUS family member 1 (RUSF1) RUS1 family Q96GQ5
Sideroflexin-3 (SFXN3) Sideroflexin family Q9BWM7
Sideroflexin-5 (SFXN5) Sideroflexin family Q8TD22
Small cell adhesion glycoprotein (SMAGP) SMAGP family Q0VAQ4
Single-pass membrane and coiled-coil domain-containing protein 4 (SMCO4) SMCO4 family Q9NRQ5
Vesicle-associated membrane protein 4 (VAMP4) Synaptobrevin family O75379
Vesicle-trafficking protein SEC22b (SEC22B) Synaptobrevin family O75396
Syntaxin-12 (STX12) Syntaxin family Q86Y82
Syntaxin-1B (STX1B) Syntaxin family P61266
Syntaxin-7 (STX7) Syntaxin family O15400
Syntaxin-8 (STX8) Syntaxin family Q9UNK0
Protein SYS1 homolog (SYS1) SYS1 family Q8N2H4
Bone morphogenetic protein 10 (BMP10) TGF-beta family O95393
Transmembrane protein 11, mitochondrial (TMEM11) TMEM11 family P17152
Transmembrane protein 120B (TMEM120B) TMEM120 family A0PK00
Transmembrane protein 229B (TMEM229B) TMEM229 family Q8NBD8
Transmembrane protein 243 (TMEM243) TMEM243 family Q9BU79
Receptor-transporting protein 2 (RTP2) TMEM7 family Q5QGT7
Protein unc-50 homolog (UNC50) Unc-50 family Q53HI1
Vesicle transport protein USE1 (USE1) USE1 family Q9NZ43
Protein YIF1A (YIF1A) YIF1 family O95070
Protein YIPF4 (YIPF4) YIP1 family Q9BSR8
Protein YIPF6 (YIPF6) YIP1 family Q96EC8
Immediate early response 3-interacting protein 1 (IER3IP1) YOS1 family Q9Y5U9
Zinc finger protein-like 1 (ZFPL1) ZFPL1 family O95159
Chondrolectin (CHODL) . Q9H9P2
Coiled-coil domain-containing protein 167 (CCDC167) . Q9P0B6
Complement C5 (C5) . P01031
DnaJ homolog subfamily C member 30, mitochondrial (DNAJC30) . Q96LL9
LEM domain-containing protein 1 (LEMD1) . Q68G75
Outer dense fiber protein 4 (ODF4) . Q2M2E3
Protein SNORC (SNORC) . Q6UX34
Small integral membrane protein 3 (SMIM3) . Q9BZL3
TBC1 domain family member 20 (TBC1D20) . Q96BZ9
Thrombomodulin (THBD) . P07204
Transmembrane protein 140 (TMEM140) . Q9NV12
Transmembrane protein 222 (TMEM222) . Q9H0R3
Transmembrane protein 254 (TMEM254) . Q8TBM7
Transmembrane protein 42 (TMEM42) . Q69YG0
Transmembrane protein 51 (TMEM51) . Q9NW97
Uncharacterized protein C5orf46 (C5orf46) . Q6UWT4

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Tamoxifen Small molecular drug DB00675
Phase 2
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Sr-31747 Small molecular drug D0D4JI

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
11 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
12 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.