Details of the Target

General Information of Target

Target ID LDTP11277
Target Name Tubulin beta-2B chain (TUBB2B)
Gene Name TUBB2B
Gene ID 347733
Synonyms
Tubulin beta-2B chain
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATPVVTKTAWKLQEIVAHASNVSSLVLGKASGRLLATGGDDCRVNLWSINKPNCIMSLT
GHTSPVESVRLNTPEELIVAGSQSGSIRVWDLEAAKILRTLMGHKANICSLDFHPYGEFV
ASGSQDTNIKLWDIRRKGCVFRYRGHSQAVRCLRFSPDGKWLASAADDHTVKLWDLTAGK
MMSEFPGHTGPVNVVEFHPNEYLLASGSSDRTIRFWDLEKFQVVSCIEGEPGPVRSVLFN
PDGCCLYSGCQDSLRVYGWEPERCFDVVLVNWGKVADLAICNDQLIGVAFSQSNVSSYVV
DLTRVTRTGTVARDPVQDHRPLAQPLPNPSAPLRRIYERPSTTCSKPQRVKQNSESERRS
PSSEDDRDERESRAEIQNAEDYNEIFQPKNSISRTPPRRSEPFPAPPEDDAATAKEAAKP
SPAMDVQFPVPNLEVLPRPPVVASTPAPKAEPAIIPATRNEPIGLKASDFLPAVKIPQQA
ELVDEDAMSQIRKGHDTMCVVLTSRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVVVDL
LNIVNQKASLWKLDLCTTVLPQIEKLLQSKYESYVQTGCTSLKLILQRFLPLITDMLAAP
PSVGVDISREERLHKCRLCYKQLKSISGLVKSKSGLSGRHGSTFRELHLLMASLD
Target Bioclass
Other
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. Plays a critical role in proper axon guidance in both central and peripheral axon tracts. Implicated in neuronal migration.
Uniprot ID
Q9BVA1
Ensemble ID
ENST00000259818.8
HGNC ID
HGNC:30829
ChEMBL ID
CHEMBL2095182

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y36(20.00); Y51(20.00); Y59(20.00); Y310(15.87)  LDD0257  [2]
TH214
 Probe Info  		Y51(9.48); Y36(9.08); Y310(8.46)  LDD0258  [2]
TH216
 Probe Info  		Y36(20.00); Y51(20.00); Y159(14.36); Y310(10.30)  LDD0259  [2]
AZ-9
 Probe Info  		D74(0.84); E383(0.90)  LDD2208  [3]
AHL-Pu-1
 Probe Info  		C303(2.15)  LDD0168  [4]
HHS-482
 Probe Info  		Y159(0.80); Y310(0.94); Y36(0.89); Y50(1.06)  LDD0285  [5]
HHS-475
 Probe Info  		Y222(0.69); Y106(0.78); Y281(0.79); Y340(0.90)  LDD0264  [6]
HHS-465
 Probe Info  		Y106(10.00); Y159(8.69); Y183(10.00); Y200(3.29)  LDD2237  [7]
DBIA
 Probe Info  		C127(1.42); C129(1.42)  LDD0078  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		C127(0.00); C129(0.00)  LDD0038  [9]
IA-alkyne
 Probe Info  		C12(0.00); C201(0.00); C354(0.00); C239(0.00)  LDD0032  [10]
Lodoacetamide azide
 Probe Info  		C127(0.00); C129(0.00)  LDD0037  [9]
JW-RF-010
 Probe Info  		C239(0.00); C12(0.00); C129(0.00); C303(0.00)  LDD0026  [11]
1d-yne
 Probe Info  		K216(0.00); K362(0.00); K252(0.00); K103(0.00)  LDD0356  [12]
IPM
 Probe Info  		C127(0.00); C129(0.00)  LDD2156  [13]
SF
 Probe Info  		Y59(0.00); Y222(0.00); Y50(0.00); Y281(0.00)  LDD0028  [14]
VSF
 Probe Info  		C239(0.00); C12(0.00); C129(0.00)  LDD0007  [15]
Phosphinate-6
 Probe Info  		C201(0.00); C12(0.00); C211(0.00); C354(0.00)  LDD0018  [16]
AOyne
 Probe Info  		15.00  LDD0443  [17]
PAL-AfBPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C049
 Probe Info  		9.99  LDD1747  [18]
C159
 Probe Info  		29.65  LDD1839  [18]
C198
 Probe Info  		69.55  LDD1874  [18]
C212
 Probe Info  		5.90  LDD1886  [18]
C213
 Probe Info  		19.03  LDD1887  [18]
C218
 Probe Info  		13.36  LDD1892  [18]
C252
 Probe Info  		9.85  LDD1925  [18]
C255
 Probe Info  		15.89  LDD1928  [18]
C264
 Probe Info  		99.73  LDD1935  [18]
C269
 Probe Info  		5.94  LDD1939  [18]
C270
 Probe Info  		15.56  LDD1940  [18]
C287
 Probe Info  		21.71  LDD1957  [18]
C349
 Probe Info  		9.85  LDD2010  [18]
C378
 Probe Info  		5.94  LDD2037  [18]
C382
 Probe Info  		28.44  LDD2041  [18]
C390
 Probe Info  		22.16  LDD2049  [18]
C413
 Probe Info  		13.18  LDD2069  [18]
C424
 Probe Info  		11.63  LDD2079  [18]
FFF probe11
 Probe Info  		20.00  LDD0472  [19]
FFF probe13
 Probe Info  		9.87  LDD0475  [19]
FFF probe3
 Probe Info  		10.48  LDD0464  [19]
FFF probe6
 Probe Info  		9.92  LDD0467  [19]
JN0003
 Probe Info  		9.71  LDD0469  [19]
A-DA
 Probe Info  		2.31  LDD0143  [20]
Kambe_55
 Probe Info  		3.04  LDD0129  [21]
DA-2
 Probe Info  		N.A.  LDD0071  [22]
OEA-DA
 Probe Info  		5.72  LDD0046  [23]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C303(2.15)  LDD0168  [4]
 LDCM0156  Aniline NCI-H1299 11.71  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C127(1.42); C129(1.42)  LDD0078  [8]
 LDCM0083  Avasimibe A-549 2.31  LDD0143  [20]
 LDCM0189  Compound 16 HEK-293T 10.67  LDD0492  [19]
 LDCM0182  Compound 18 HEK-293T 7.06  LDD0501  [19]
 LDCM0191  Compound 21 HEK-293T 7.60  LDD0493  [19]
 LDCM0190  Compound 34 HEK-293T 11.92  LDD0497  [19]
 LDCM0192  Compound 35 HEK-293T 6.64  LDD0491  [19]
 LDCM0193  Compound 36 HEK-293T 5.33  LDD0494  [19]
 LDCM0194  Compound 37 HEK-293T 9.14  LDD0498  [19]
 LDCM0195  Compound 38 HEK-293T 7.03  LDD0499  [19]
 LDCM0196  Compound 39 HEK-293T 6.54  LDD0496  [19]
 LDCM0197  Compound 40 HEK-293T 6.05  LDD0495  [19]
 LDCM0181  Compound 41 HEK-293T 8.06  LDD0502  [19]
 LDCM0183  Compound 42 HEK-293T 4.58  LDD0500  [19]
 LDCM0625  F8 Ramos 0.86  LDD2187  [24]
 LDCM0572  Fragment10 Ramos 0.26  LDD2189  [24]
 LDCM0573  Fragment11 Ramos 0.16  LDD2190  [24]
 LDCM0574  Fragment12 Ramos 0.32  LDD2191  [24]
 LDCM0575  Fragment13 Ramos 0.49  LDD2192  [24]
 LDCM0576  Fragment14 Ramos 1.61  LDD2193  [24]
 LDCM0579  Fragment20 Ramos 0.73  LDD2194  [24]
 LDCM0580  Fragment21 Ramos 0.47  LDD2195  [24]
 LDCM0582  Fragment23 Ramos 0.69  LDD2196  [24]
 LDCM0578  Fragment27 Ramos 0.77  LDD2197  [24]
 LDCM0586  Fragment28 Ramos 1.20  LDD2198  [24]
 LDCM0588  Fragment30 Ramos 0.53  LDD2199  [24]
 LDCM0589  Fragment31 Ramos 0.48  LDD2200  [24]
 LDCM0590  Fragment32 Ramos 0.48  LDD2201  [24]
 LDCM0468  Fragment33 Ramos 0.39  LDD2202  [24]
 LDCM0596  Fragment38 Ramos 0.27  LDD2203  [24]
 LDCM0566  Fragment4 Ramos 0.72  LDD2184  [24]
 LDCM0610  Fragment52 Ramos 0.44  LDD2204  [24]
 LDCM0614  Fragment56 Ramos 0.67  LDD2205  [24]
 LDCM0569  Fragment7 Ramos 0.68  LDD2186  [24]
 LDCM0571  Fragment9 Ramos 0.51  LDD2188  [24]
 LDCM0116  HHS-0101 DM93 Y222(0.69); Y106(0.78); Y281(0.79); Y340(0.90)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y222(0.59); Y106(0.68); Y281(0.80); Y340(0.92)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y222(0.62); Y106(0.66); Y281(0.85); Y340(0.89)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y222(0.63); Y106(0.63); Y281(0.91); Y340(1.26)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y106(0.62); Y222(0.63); Y281(0.76); Y340(1.21)  LDD0268  [6]
 LDCM0123  JWB131 DM93 Y159(0.80); Y310(0.94); Y36(0.89); Y50(1.06)  LDD0285  [5]
 LDCM0124  JWB142 DM93 Y159(0.94); Y310(0.75); Y36(0.63); Y50(0.96)  LDD0286  [5]
 LDCM0125  JWB146 DM93 Y159(0.67); Y310(1.49); Y36(1.11); Y50(1.26)  LDD0287  [5]
 LDCM0126  JWB150 DM93 Y159(3.60); Y310(2.86); Y36(2.34); Y50(1.54)  LDD0288  [5]
 LDCM0127  JWB152 DM93 Y159(1.27); Y310(1.95); Y36(1.64); Y50(1.90)  LDD0289  [5]
 LDCM0128  JWB198 DM93 Y159(0.40); Y310(1.16); Y36(1.12); Y50(1.75)  LDD0290  [5]
 LDCM0129  JWB202 DM93 Y159(0.61); Y310(0.46); Y36(0.44); Y51(0.50)  LDD0291  [5]
 LDCM0130  JWB211 DM93 Y159(0.62); Y310(1.05); Y36(0.94); Y50(1.06)  LDD0292  [5]
 LDCM0073  Kambe_cp3 PC-3 3.04  LDD0129  [21]
 LDCM0022  KB02 HEK-293T C354(0.93); C12(0.93); C239(0.95); C127(0.94)  LDD1492  [25]
 LDCM0023  KB03 Jurkat C127,C129(5.42)  LDD0315  [10]
 LDCM0024  KB05 HEK-293T C354(0.88); C12(1.00); C239(0.95); C127(1.03)  LDD1502  [25]
 LDCM0627  NUDT7-COV-1 HEK-293T C127(15.00); C129(15.00); C239(1.43); C12(1.05)  LDD2206  [26]
 LDCM0628  OTUB2-COV-1 HEK-293T C129(14.14); C239(1.14); C201(1.01); C211(1.01)  LDD2207  [26]
 LDCM0131  RA190 MM1.R C129(1.28)  LDD0304  [27]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Voltage-dependent P/Q-type calcium channel subunit alpha-1A (CACNA1A) Calcium channel alpha-1 subunit (TC 1.A.1.11) family O00555

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2-mercapto-n-[12310-tetramethoxy-9-oxo-5679-tetrahydro-benzo[A]Heptalen-7-yl]Acetamide . DB07574
Cyt997 . DB05147

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
14 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
15 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
16 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
17 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
18 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
19 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
20 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
21 Mapping the protein interaction landscape for fully functionalized small-molecule probes in human cells. J Am Chem Soc. 2014 Jul 30;136(30):10777-82. doi: 10.1021/ja505517t. Epub 2014 Jul 21.
22 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
23 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
26 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
27 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.