Probe Name	C212					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP05758	S-methyl-5'-thioadenosine phosphorylase (MTAP)					31.1
LDTP01803	Adenosine deaminase (ADA)					29.0
LDTP04542	Thimet oligopeptidase (THOP1)					18.9
LDTP09799	DCN1-like protein 4 (DCUN1D4)					16.6
LDTP03198	Ferrochelatase, mitochondrial (FECH)					15.5
LDTP15484	Saccharopine dehydrogenase-like oxidoreductase (SCCPDH)					15.5
LDTP03787	Choline kinase alpha (CHKA)					14.0
LDTP03540	Heme oxygenase 2 (HMOX2)					10.5
LDTP06333	Serum paraoxonase/arylesterase 2 (PON2)					9.6
LDTP05920	Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G)					8.9
LDTP01518	NADH dehydrogenase 1 alpha subcomplex subunit 7 (NDUFA7)					8.9
LDTP04565	Methionine aminopeptidase 1 (METAP1)					8.3
LDTP15422	Prostaglandin reductase 3 (PTGR3)					8.3
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					7.7
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					7.7
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					7.5
LDTP02175	Epoxide hydrolase 1 (EPHX1)					7.3
LDTP10329	Ceramide synthase 2 (CERS2)					7.3
LDTP03671	Multidrug resistance-associated protein 1 (ABCC1)					7.0
LDTP05128	Glutathione S-transferase omega-1 (GSTO1)					6.8
LDTP11273	N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1)					6.4
LDTP12013	dCTP pyrophosphatase 1 (DCTPP1)					6.3
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					6.3
LDTP09095	Diacylglycerol lipase-beta (DAGLB)					6.3
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					6.2
LDTP05724	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1)					6.1
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					6.0
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					5.9
LDTP13620	Lysosomal thioesterase PPT2 (PPT2)					5.8
LDTP12363	Lysophosphatidic acid phosphatase type 6 (ACP6)					5.7
LDTP10550	Dehydrogenase/reductase SDR family member 1 (DHRS1)					5.6
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					5.5
LDTP01687	Apoptosis-inducing factor 1, mitochondrial (AIFM1)					5.5
LDTP15480	Prenylcysteine oxidase-like (PCYOX1L)					5.5
LDTP00575	Glutathione S-transferase A4 (GSTA4)					5.4
LDTP00832	NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3)					5.4
LDTP13200	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (FTSJ1)					5.3
LDTP17247	5'-nucleotidase domain-containing protein 1 (NT5DC1)					5.3

Target ID	Target Name					Binding Ratio
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					68.1
LDTP03061	Cation-dependent mannose-6-phosphate receptor (M6PR)					15.1
LDTP02215	Prosaposin (PSAP)					9.5
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					9.3
LDTP02071	Amyloid-beta precursor protein (APP)					8.2
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					7.8
LDTP16190	Protein FAM8A1 (FAM8A1)					7.2
LDTP04789	Synaptojanin-2-binding protein (SYNJ2BP)					7.1
LDTP05236	Phosphatidylinositol transfer protein alpha isoform (PITPNA)					7.0
LDTP11813	Solute carrier family 25 member 32 (SLC25A32)					6.5
LDTP00592	Surfeit locus protein 4 (SURF4)					6.4
LDTP11406	Transmembrane protein 59 (TMEM59)					6.4
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					6.3
LDTP13256	SUN domain-containing protein 2 (SUN2)					6.1
LDTP07236	Protein GPR107 (GPR107)					6.1
LDTP17641	Solute carrier family 35 member F2 (SLC35F2)					6.0
LDTP02544	Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)					5.9
LDTP14952	Mpv17-like protein 2 (MPV17L2)					5.6
LDTP15733	Solute carrier family 25 member 44 (SLC25A44)					5.5
LDTP00310	Syntenin-1 (SDCBP)					5.5
LDTP15481	Transmembrane protein 87A (TMEM87A)					5.5
LDTP16813	Tetraspanin-3 (TSPAN3)					5.4

Target ID	Target Name					Binding Ratio
LDTP05065	Y-box-binding protein 1 (YBX1)					6.7

Target ID	Target Name					Binding Ratio
LDTP00105	Protein unc-119 homolog B (UNC119B)					14.9
LDTP15623	Neuferricin (CYB5D2)					14.3
LDTP13630	Neudesin (NENF)					10.1
LDTP13606	Mammalian ependymin-related protein 1 (EPDR1)					9.5
LDTP08154	Large ribosomal subunit protein uL10m (MRPL10)					8.6
LDTP04094	Large proline-rich protein BAG6 (BAG6)					8.1
LDTP01075	Protein CutA (CUTA)					7.7
LDTP13828	Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3)					7.3
LDTP19372	Tetratricopeptide repeat protein 38 (TTC38)					7.1
LDTP05160	Nucleobindin-2 (NUCB2)					6.5
LDTP13755	Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2)					6.3
LDTP10281	Cytoplasmic dynein 2 intermediate chain 2 (DYNC2I2)					6.3
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					6.2
LDTP02443	Calmodulin-3 (CALM3)					6.2
LDTP11978	HAUS augmin-like complex subunit 4 (HAUS4)					6.0
LDTP13210	Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1)					6.0
LDTP11277	Tubulin beta-2B chain (TUBB2B)					5.9
LDTP05226	RNA-binding protein 3 (RBM3)					5.7
LDTP02205	Tubulin beta chain (TUBB)					5.6
LDTP02351	Tropomyosin alpha-1 chain (TPM1)					5.6
LDTP14486	Clustered mitochondria protein homolog (CLUH)					5.4
LDTP02297	Vimentin (VIM)					5.3
LDTP13170	COP9 signalosome complex subunit 7a (COPS7A)					5.2
LDTP08689	Dyslexia-associated protein KIAA0319-like protein (KIAA0319L)					5.2
LDTP00871	Microtubule nucleation factor SSNA1 (SSNA1)					5.2
LDTP08130	Rab9 effector protein with kelch motifs (RABEPK)					5.2
LDTP13214	UPF0449 protein C19orf25 (C19orf25)					5.2

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587