Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C252  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 92 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 78.2
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 53.4
 LDTP00697  Cytochrome b5 type B (CYB5B) 41.9
 LDTP03540  Heme oxygenase 2 (HMOX2) 41.1
 LDTP10329  Ceramide synthase 2 (CERS2) 32.7
 LDTP08565  E3 ubiquitin-protein ligase UBR2 (UBR2) 31.8
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 31.1
 LDTP03842  Squalene synthase (FDFT1) 30.7
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 30.5
 LDTP11127  ADP-dependent glucokinase (ADPGK) 29.4
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 28.8
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 28.1
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 25.5
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 25.3
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 24.8
 LDTP02359  Heme oxygenase 1 (HMOX1) 24.1
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 23.6
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 23.4
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 22.3
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 21.4
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 19.6
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 18.1
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 17.6
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 17.4
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 17.0
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 16.4
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 16.4
 LDTP15328  Dolichyldiphosphatase 1 (DOLPP1) 15.2
 LDTP06618  ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) 15.0
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 14.4
 LDTP11187  COP9 signalosome complex subunit 4 (COPS4) 14.2
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 13.9
 LDTP03912  Trifunctional enzyme subunit alpha, mitochondrial (HADHA) 13.5
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 13.4
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 13.0
 LDTP13751  Exonuclease 1 (EXO1) 12.9
 LDTP02385  Leukotriene A-4 hydrolase (LTA4H) 12.9
 LDTP03419  Proteasome subunit beta type-5 (PSMB5) 12.8
 LDTP02254  ATP-dependent translocase ABCB1 (ABCB1) 12.7
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 12.7
 LDTP04358  Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) 12.6
 LDTP06849  Prolyl endopeptidase-like (PREPL) 12.6
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 12.6
 LDTP01769  Dihydrofolate reductase (DHFR) 12.3
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 12.2
 LDTP01641  STAM-binding protein (STAMBP) 12.1
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 12.0
 LDTP04581  Holocytochrome c-type synthase (HCCS) 11.8
 LDTP10612  Lysophospholipid acyltransferase 7 (MBOAT7) 11.6
 LDTP03145  Catechol O-methyltransferase (COMT) 11.4
 LDTP12790  Glutaminyl-peptide cyclotransferase-like protein (QPCTL) 11.1
 LDTP18414  5'-nucleotidase domain-containing protein 2 (NT5DC2) 10.6
 LDTP06617  Ceramide glucosyltransferase (UGCG) 10.6
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 10.6
 LDTP10020  Ras-related protein Rab-24 (RAB24) 10.3
 LDTP04689  Adenosine kinase (ADK) 10.3
 LDTP02860  Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) 10.1
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 10.1
 LDTP06270  Signal peptidase complex subunit 2 (SPCS2) 10.0
 LDTP09874  Endoplasmic reticulum protein SC65 (P3H4) 9.9
 LDTP07990  Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) 9.9
 LDTP03787  Choline kinase alpha (CHKA) 9.8
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 9.7
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 9.6
 LDTP06150  DNA replication licensing factor MCM6 (MCM6) 9.5
 LDTP13358  Leucyl-cystinyl aminopeptidase (LNPEP) 9.5
 LDTP03697  Mannose-6-phosphate isomerase (MPI) 9.4
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 9.4
 LDTP06142  Squalene monooxygenase (SQLE) 9.4
 LDTP03854  Vitamin K-dependent gamma-carboxylase (GGCX) 9.4
 LDTP08666  Xaa-Arg dipeptidase (PM20D2) 9.4
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 9.3
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 9.2
 LDTP13375  2-hydroxyacyl-CoA lyase 1 (HACL1) 9.1
 LDTP06303  Kinesin-like protein KIF14 (KIF14) 9.0
 LDTP02679  Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) 9.0
 LDTP04284  Proteasome subunit beta type-3 (PSMB3) 9.0
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 8.9
 LDTP12867  Very-long-chain enoyl-CoA reductase (TECR) 8.9
 LDTP07154  ATPase family AAA domain-containing protein 3B (ATAD3B) 8.8
 LDTP10495  Ubiquitin carboxyl-terminal hydrolase 47 (USP47) 8.8
 LDTP12586  Phenylalanine--tRNA ligase beta subunit (FARSB) 8.8
 LDTP12720  Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) 8.7
 LDTP12040  Prostaglandin E synthase 2 (PTGES2) 8.7
 LDTP05408  Antigen peptide transporter 1 (TAP1) 8.6
 LDTP02713  Macrophage migration inhibitory factor (MIF) 8.5
 LDTP07003  Presequence protease, mitochondrial (PITRM1) 8.5
 LDTP00988  Protein O-GlcNAcase (OGA) 8.5
 LDTP04893  Ras-related protein Rab-5B (RAB5B) 8.5
 LDTP12020  Histone-lysine N-methyltransferase SMYD3 (SMYD3) 8.3
 LDTP09188  Lysophosphatidylcholine acyltransferase 1 (LPCAT1) 8.3
 LDTP11823  Peptidyl-prolyl cis-trans isomerase-like 3 (PPIL3) 8.3
Transporter and channel
Click to Show/Hide to Show 53 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 99.7
 LDTP01804  ATP synthase subunit a (MT-ATP6) 52.3
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 42.2
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 39.7
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 32.9
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 31.8
 LDTP06633  Reticulon-1 (RTN1) 31.3
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 29.0
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 23.8
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 23.4
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 20.8
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 19.8
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 19.7
 LDTP07236  Protein GPR107 (GPR107) 19.7
 LDTP20002  Transmembrane protein 160 (TMEM160) 19.2
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 18.1
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 17.9
 LDTP18153  Major facilitator superfamily domain-containing protein 3 (MFSD3) 17.5
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 16.7
 LDTP05900  Metaxin-1 (MTX1) 16.3
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 16.2
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 15.7
 LDTP16190  Protein FAM8A1 (FAM8A1) 15.0
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 14.1
 LDTP11213  Nucleoporin NDC1 (NDC1) 13.8
 LDTP08878  ER membrane protein complex subunit 5 (MMGT1) 13.6
 LDTP01060  PRA1 family protein 2 (PRAF2) 13.6
 LDTP11634  Derlin-2 (DERL2) 13.0
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 12.9
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 12.6
 LDTP00235  Membrane-associated progesterone receptor component 1 (PGRMC1) 12.0
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 11.8
 LDTP11245  Derlin-1 (DERL1) 10.8
 LDTP10343  Vacuole membrane protein 1 (VMP1) 10.6
 LDTP10059  PAT complex subunit CCDC47 (CCDC47) 10.5
 LDTP10065  Transmembrane protein 230 (TMEM230) 10.5
 LDTP10669  Melanoma inhibitory activity protein 2 (MIA2) 10.3
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 10.3
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 9.8
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 9.6
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 9.5
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 9.5
 LDTP11095  Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) 9.4
 LDTP07058  Transmembrane protein 201 (TMEM201) 9.2
 LDTP12909  Mitochondrial carrier homolog 1 (MTCH1) 9.1
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 8.9
 LDTP17362  Magnesium transporter NIPA3 (NIPAL1) 8.8
 LDTP01454  SUN domain-containing protein 1 (SUN1) 8.7
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 8.6
 LDTP08030  Autophagy-related protein 9A (ATG9A) 8.5
 LDTP12210  Mitochondrial thiamine pyrophosphate carrier (SLC25A19) 8.5
 LDTP00821  Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) 8.4
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 8.3
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP10501  DnaJ homolog subfamily C member 1 (DNAJC1) 9.1
Other
Click to Show/Hide to Show 49 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 76.1
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 35.8
 LDTP01522  Reticulon-3 (RTN3) 29.9
 LDTP12412  Reticulon-4 (RTN4) 26.0
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 25.6
 LDTP05960  Trophoblast glycoprotein (TPBG) 25.5
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 22.0
 LDTP00105  Protein unc-119 homolog B (UNC119B) 21.3
 LDTP16006  Optic atrophy 3 protein (OPA3) 21.1
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 20.0
 LDTP05160  Nucleobindin-2 (NUCB2) 19.6
 LDTP16080  Protein FAM114A2 (FAM114A2) 19.0
 LDTP10056  Protein FAM162A (FAM162A) 18.5
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 18.0
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 17.4
 LDTP09970  RNA-binding protein with multiple splicing (RBPMS) 17.1
 LDTP19963  TraB domain-containing protein (TRABD) 15.5
 LDTP04853  Eukaryotic translation initiation factor 3 subunit E (EIF3E) 13.2
 LDTP05375  Nucleobindin-1 (NUCB1) 13.2
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 12.8
 LDTP15623  Neuferricin (CYB5D2) 12.6
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 12.2
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 12.0
 LDTP15741  Cytochrome c oxidase assembly protein COX14 (COX14) 11.3
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 11.1
 LDTP08606  Nurim (NRM) 11.1
 LDTP00859  Synaptogyrin-2 (SYNGR2) 10.9
 LDTP01652  Centrosomal protein 43 (CEP43) 10.9
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 10.9
 LDTP13086  Syntaxin-18 (STX18) 10.9
 LDTP01494  Protein YIF1A (YIF1A) 10.8
 LDTP05089  Immunoglobulin-binding protein 1 (IGBP1) 10.6
 LDTP05318  RNA-binding protein EWS (EWSR1) 10.6
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 10.5
 LDTP00716  RNA binding protein fox-1 homolog 2 (RBFOX2) 10.3
 LDTP04652  Eukaryotic translation initiation factor 5 (EIF5) 10.0
 LDTP11978  HAUS augmin-like complex subunit 4 (HAUS4) 9.9
 LDTP11277  Tubulin beta-2B chain (TUBB2B) 9.8
 LDTP16078  Heme-binding protein 1 (HEBP1) 9.8
 LDTP19780  Keratinocyte-associated transmembrane protein 2 (KCT2) 9.8
 LDTP14058  Rap guanine nucleotide exchange factor 2 (RAPGEF2) 9.6
 LDTP10357  RUS family member 1 (RUSF1) 9.6
 LDTP02869  Stathmin (STMN1) 9.6
 LDTP03968  Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO) 9.5
 LDTP00417  Programmed cell death protein 5 (PDCD5) 9.0
 LDTP08224  Protein LYRIC (MTDH) 9.0
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 8.8
 LDTP05257  Receptor expression-enhancing protein 5 (REEP5) 8.7
 LDTP02297  Vimentin (VIM) 8.4

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587