Details of the Target

General Information of Target

Target ID LDTP05318
Target Name RNA-binding protein EWS (EWSR1)
Gene Name EWSR1
Gene ID 2130
Synonyms
EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTAT
YGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGT
QPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSY
PPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNR
GRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDN
SAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDP
PTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGLPPREGRGMPPPLRGGPGGPGGP
GGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTEC
NQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFR
GGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY
Target Bioclass
Other
Family
RRM TET family
Subcellular location
Nucleus
Function
Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Uniprot ID
Q01844
Ensemble ID
ENST00000332035.10
HGNC ID
HGNC:3508
ChEMBL ID
CHEMBL3351202

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.C543R DBIA    Probe Info 
HCT15 SNV: p.Y208C .
JURKAT SNV: p.Q280Ter .
MOLT4 SNV: p.G601V IA-alkyne    Probe Info 
REH SNV: p.S443A DBIA    Probe Info 
SH4 SNV: p.S274R DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 30 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		16.87  LDD0066  [1]
TH211
 Probe Info  		Y278(5.38)  LDD0257  [2]
TH216
 Probe Info  		Y278(20.00)  LDD0259  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
STPyne
 Probe Info  		K433(1.03); K439(7.14); K441(6.21)  LDD0277  [4]
BTD
 Probe Info  		C384(2.57)  LDD1699  [5]
AZ-9
 Probe Info  		E428(0.78)  LDD2208  [6]
IPM
 Probe Info  		C540(0.00); C543(0.00)  LDD0241  [7]
OPA-S-S-alkyne
 Probe Info  		K644(1.76)  LDD3494  [8]
Probe 1
 Probe Info  		Y278(12.41); Y401(8.74); Y417(57.83)  LDD3495  [9]
DBIA
 Probe Info  		C389(1.92)  LDD3324  [10]
AHL-Pu-1
 Probe Info  		C540(5.47); C524(4.21)  LDD0170  [11]
HHS-482
 Probe Info  		Y278(0.88)  LDD0285  [12]
HHS-475
 Probe Info  		Y278(0.92); Y401(0.93)  LDD0264  [13]
HHS-465
 Probe Info  		Y278(10.00); Y401(10.00)  LDD2237  [14]
5E-2FA
 Probe Info  		H647(0.00); H271(0.00); H399(0.00)  LDD2235  [15]
ATP probe
 Probe Info  		K408(0.00); K410(0.00); K404(0.00)  LDD0199  [16]
m-APA
 Probe Info  		H271(0.00); H399(0.00)  LDD2231  [15]
4-Iodoacetamidophenylacetylene
 Probe Info  		C529(0.00); C524(0.00); C540(0.00); C543(0.00)  LDD0038  [17]
IA-alkyne
 Probe Info  		C540(0.00); C543(0.00)  LDD0036  [17]
Lodoacetamide azide
 Probe Info  		C540(0.00); C543(0.00)  LDD0037  [17]
NHS
 Probe Info  		N.A.  LDD0010  [18]
SF
 Probe Info  		Y401(0.00); K441(0.00)  LDD0028  [19]
TFBX
 Probe Info  		C543(0.00); C540(0.00)  LDD0148  [20]
Ox-W18
 Probe Info  		N.A.  LDD2175  [21]
1c-yne
 Probe Info  		N.A.  LDD0228  [22]
Acrolein
 Probe Info  		N.A.  LDD0217  [23]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [23]
Methacrolein
 Probe Info  		N.A.  LDD0218  [23]
W1
 Probe Info  		C540(0.00); C543(0.00); C529(0.00); C524(0.00)  LDD0236  [7]
PAL-AfBPP Probe
Click To Hide/Show 30 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		5.50  LDD1714  [24]
C027
 Probe Info  		5.74  LDD1733  [24]
C040
 Probe Info  		6.63  LDD1740  [24]
C087
 Probe Info  		8.34  LDD1779  [24]
C106
 Probe Info  		16.68  LDD1793  [24]
C108
 Probe Info  		8.17  LDD1795  [24]
C165
 Probe Info  		16.91  LDD1845  [24]
C196
 Probe Info  		10.85  LDD1872  [24]
C197
 Probe Info  		5.43  LDD1873  [24]
C213
 Probe Info  		15.56  LDD1887  [24]
C232
 Probe Info  		41.07  LDD1905  [24]
C249
 Probe Info  		13.09  LDD1922  [24]
C252
 Probe Info  		10.56  LDD1925  [24]
C313
 Probe Info  		14.22  LDD1980  [24]
C314
 Probe Info  		12.73  LDD1981  [24]
C338
 Probe Info  		10.13  LDD2001  [24]
C348
 Probe Info  		11.24  LDD2009  [24]
C350
 Probe Info  		22.16  LDD2011  [24]
C353
 Probe Info  		5.94  LDD2014  [24]
C363
 Probe Info  		16.34  LDD2024  [24]
C407
 Probe Info  		11.39  LDD2064  [24]
C420
 Probe Info  		9.99  LDD2075  [24]
FFF probe11
 Probe Info  		14.91  LDD0471  [25]
FFF probe13
 Probe Info  		20.00  LDD0475  [25]
FFF probe14
 Probe Info  		20.00  LDD0477  [25]
FFF probe2
 Probe Info  		19.37  LDD0463  [25]
FFF probe3
 Probe Info  		13.88  LDD0465  [25]
FFF probe6
 Probe Info  		5.11  LDD0468  [25]
JN0003
 Probe Info  		20.00  LDD0469  [25]
Photonaproxen
 Probe Info  		N.A.  LDD0157  [26]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C384(0.44); C543(0.45); C540(0.41)  LDD2142  [5]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C384(0.68); C543(0.42); C540(0.37)  LDD2112  [5]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C543(0.60); C540(0.93)  LDD2117  [5]
 LDCM0025  4SU-RNA DM93 C540(5.47); C524(4.21)  LDD0170  [11]
 LDCM0214  AC1 HEK-293T C529(0.98); C524(0.80); C543(0.97)  LDD1507  [27]
 LDCM0215  AC10 HEK-293T C529(0.95); C524(0.89); C543(1.13)  LDD1508  [27]
 LDCM0226  AC11 HEK-293T C529(1.02); C524(0.90); C543(1.03)  LDD1509  [27]
 LDCM0237  AC12 HEK-293T C529(1.08); C524(1.11); C543(1.00)  LDD1510  [27]
 LDCM0259  AC14 HEK-293T C529(0.80); C524(1.01); C543(1.05)  LDD1512  [27]
 LDCM0270  AC15 HEK-293T C529(0.95); C524(1.01); C543(1.03)  LDD1513  [27]
 LDCM0276  AC17 HEK-293T C529(0.96); C524(0.93); C543(1.00)  LDD1515  [27]
 LDCM0277  AC18 HEK-293T C529(0.94); C524(0.92); C543(1.03)  LDD1516  [27]
 LDCM0278  AC19 HEK-293T C529(0.47); C524(0.51); C543(0.62)  LDD1517  [27]
 LDCM0279  AC2 HEK-293T C529(0.96); C524(0.85); C543(0.94)  LDD1518  [27]
 LDCM0280  AC20 HEK-293T C529(1.03); C524(0.98); C543(0.99)  LDD1519  [27]
 LDCM0281  AC21 HEK-293T C529(0.93); C524(1.00); C543(0.99)  LDD1520  [27]
 LDCM0282  AC22 HEK-293T C529(0.99); C524(0.98); C543(0.99)  LDD1521  [27]
 LDCM0283  AC23 HEK-293T C529(0.88); C524(1.02); C543(1.06)  LDD1522  [27]
 LDCM0284  AC24 HEK-293T C529(0.91); C524(1.03); C543(0.98)  LDD1523  [27]
 LDCM0285  AC25 HEK-293T C529(1.00); C524(0.94); C543(0.97)  LDD1524  [27]
 LDCM0286  AC26 HEK-293T C529(0.92); C524(0.98); C543(1.03)  LDD1525  [27]
 LDCM0287  AC27 HEK-293T C529(0.82); C524(0.92); C543(0.95)  LDD1526  [27]
 LDCM0288  AC28 HEK-293T C529(1.08); C524(0.96); C543(0.96)  LDD1527  [27]
 LDCM0289  AC29 HEK-293T C529(1.10); C524(0.99); C543(0.93)  LDD1528  [27]
 LDCM0290  AC3 HEK-293T C529(0.91); C524(0.81); C543(0.94)  LDD1529  [27]
 LDCM0291  AC30 HEK-293T C529(0.99); C524(0.99); C543(1.03)  LDD1530  [27]
 LDCM0292  AC31 HEK-293T C529(0.96); C524(0.99); C543(0.98)  LDD1531  [27]
 LDCM0293  AC32 HEK-293T C529(0.97); C524(0.94); C543(1.05)  LDD1532  [27]
 LDCM0294  AC33 HEK-293T C529(1.04); C524(0.91); C543(1.08)  LDD1533  [27]
 LDCM0295  AC34 HEK-293T C529(0.84); C524(0.93); C543(1.02)  LDD1534  [27]
 LDCM0296  AC35 HEK-293T C529(1.03); C524(0.83); C543(1.00)  LDD1535  [27]
 LDCM0297  AC36 HEK-293T C529(1.11); C524(0.98); C543(1.02)  LDD1536  [27]
 LDCM0298  AC37 HEK-293T C529(0.95); C524(1.11); C543(1.03)  LDD1537  [27]
 LDCM0299  AC38 HEK-293T C529(1.14); C524(1.04); C543(1.06)  LDD1538  [27]
 LDCM0300  AC39 HEK-293T C529(0.97); C524(1.07); C543(1.04)  LDD1539  [27]
 LDCM0301  AC4 HEK-293T C529(0.92); C524(0.98); C543(0.93)  LDD1540  [27]
 LDCM0302  AC40 HEK-293T C529(0.92); C524(1.22); C543(0.99)  LDD1541  [27]
 LDCM0303  AC41 HEK-293T C529(1.04); C524(0.99); C543(1.07)  LDD1542  [27]
 LDCM0304  AC42 HEK-293T C529(0.84); C524(0.98); C543(0.98)  LDD1543  [27]
 LDCM0305  AC43 HEK-293T C529(0.94); C524(0.92); C543(1.02)  LDD1544  [27]
 LDCM0306  AC44 HEK-293T C529(1.18); C524(0.98); C543(1.04)  LDD1545  [27]
 LDCM0307  AC45 HEK-293T C529(0.94); C524(1.07); C543(1.08)  LDD1546  [27]
 LDCM0308  AC46 HEK-293T C529(1.09); C524(1.07); C543(1.02)  LDD1547  [27]
 LDCM0309  AC47 HEK-293T C529(0.98); C524(0.93); C543(1.04)  LDD1548  [27]
 LDCM0310  AC48 HEK-293T C529(1.06); C524(0.94); C543(1.08)  LDD1549  [27]
 LDCM0311  AC49 HEK-293T C529(0.85); C524(0.89); C543(1.07)  LDD1550  [27]
 LDCM0312  AC5 HEK-293T C529(0.86); C524(0.91); C543(0.96)  LDD1551  [27]
 LDCM0313  AC50 HEK-293T C529(0.89); C524(1.01); C543(1.01)  LDD1552  [27]
 LDCM0314  AC51 HEK-293T C529(0.93); C524(0.91); C543(1.06)  LDD1553  [27]
 LDCM0315  AC52 HEK-293T C529(0.98); C524(1.00); C543(0.99)  LDD1554  [27]
 LDCM0316  AC53 HEK-293T C529(0.96); C524(0.95); C543(1.04)  LDD1555  [27]
 LDCM0317  AC54 HEK-293T C529(0.90); C524(0.95); C543(1.00)  LDD1556  [27]
 LDCM0318  AC55 HEK-293T C529(0.91); C524(1.15); C543(1.08)  LDD1557  [27]
 LDCM0319  AC56 HEK-293T C529(1.05); C524(1.02); C543(1.07)  LDD1558  [27]
 LDCM0320  AC57 HEK-293T C529(0.89); C524(0.78); C543(0.94)  LDD1559  [27]
 LDCM0321  AC58 HEK-293T C529(0.98); C524(0.91); C543(1.02)  LDD1560  [27]
 LDCM0322  AC59 HEK-293T C529(0.91); C524(0.77); C543(0.95)  LDD1561  [27]
 LDCM0323  AC6 HEK-293T C529(0.82); C524(0.88); C543(0.96)  LDD1562  [27]
 LDCM0324  AC60 HEK-293T C529(1.07); C524(0.91); C543(0.91)  LDD1563  [27]
 LDCM0325  AC61 HEK-293T C529(1.02); C524(0.87); C543(0.94)  LDD1564  [27]
 LDCM0326  AC62 HEK-293T C529(0.93); C524(0.97); C543(0.93)  LDD1565  [27]
 LDCM0327  AC63 HEK-293T C529(0.96); C524(0.96); C543(0.97)  LDD1566  [27]
 LDCM0328  AC64 HEK-293T C529(1.14); C524(1.29); C543(1.01)  LDD1567  [27]
 LDCM0334  AC7 HEK-293T C529(0.92); C524(1.01); C543(1.05)  LDD1568  [27]
 LDCM0345  AC8 HEK-293T C529(1.03); C524(1.16); C543(1.01)  LDD1569  [27]
 LDCM0520  AKOS000195272 MDA-MB-231 C384(0.71); C543(0.61); C540(0.58)  LDD2113  [5]
 LDCM0248  AKOS034007472 HEK-293T C529(0.96); C524(1.17); C543(1.03)  LDD1511  [27]
 LDCM0356  AKOS034007680 HEK-293T C529(1.04); C524(0.88); C543(1.07)  LDD1570  [27]
 LDCM0275  AKOS034007705 HEK-293T C529(0.97); C524(0.95); C543(1.08)  LDD1514  [27]
 LDCM0498  BS-3668 MDA-MB-231 C384(0.97)  LDD2091  [5]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [23]
 LDCM0367  CL1 HEK-293T C529(1.03); C524(0.96); C543(0.99)  LDD1571  [27]
 LDCM0368  CL10 HEK-293T C529(0.91); C524(0.81); C543(0.80)  LDD1572  [27]
 LDCM0369  CL100 HEK-293T C529(0.88); C524(0.97); C543(0.92)  LDD1573  [27]
 LDCM0370  CL101 HEK-293T C529(0.91); C524(0.99); C543(0.97)  LDD1574  [27]
 LDCM0371  CL102 HEK-293T C529(0.86); C524(0.83); C543(0.96)  LDD1575  [27]
 LDCM0372  CL103 HEK-293T C529(0.97); C524(0.90); C543(1.11)  LDD1576  [27]
 LDCM0373  CL104 HEK-293T C529(0.98); C524(0.91); C543(1.09)  LDD1577  [27]
 LDCM0374  CL105 HEK-293T C529(0.99); C524(0.78); C543(0.92)  LDD1578  [27]
 LDCM0375  CL106 HEK-293T C529(0.84); C524(0.98); C543(1.01)  LDD1579  [27]
 LDCM0376  CL107 HEK-293T C529(0.93); C524(0.93); C543(1.04)  LDD1580  [27]
 LDCM0377  CL108 HEK-293T C529(0.88); C524(0.88); C543(1.05)  LDD1581  [27]
 LDCM0378  CL109 HEK-293T C529(0.88); C524(0.87); C543(0.95)  LDD1582  [27]
 LDCM0379  CL11 HEK-293T C529(0.84); C524(0.94); C543(0.92)  LDD1583  [27]
 LDCM0380  CL110 HEK-293T C529(0.89); C524(0.83); C543(0.90)  LDD1584  [27]
 LDCM0381  CL111 HEK-293T C529(0.77); C524(0.81); C543(0.95)  LDD1585  [27]
 LDCM0382  CL112 HEK-293T C529(1.04); C524(0.80); C543(0.98)  LDD1586  [27]
 LDCM0383  CL113 HEK-293T C529(0.94); C524(0.89); C543(0.99)  LDD1587  [27]
 LDCM0384  CL114 HEK-293T C529(0.92); C524(0.97); C543(0.92)  LDD1588  [27]
 LDCM0385  CL115 HEK-293T C529(1.04); C524(0.87); C543(1.06)  LDD1589  [27]
 LDCM0386  CL116 HEK-293T C529(0.92); C524(1.05); C543(1.04)  LDD1590  [27]
 LDCM0387  CL117 HEK-293T C529(0.81); C524(0.99); C543(0.99)  LDD1591  [27]
 LDCM0388  CL118 HEK-293T C529(0.84); C524(0.91); C543(0.97)  LDD1592  [27]
 LDCM0389  CL119 HEK-293T C529(0.92); C524(1.13); C543(1.10)  LDD1593  [27]
 LDCM0390  CL12 HEK-293T C529(1.02); C524(0.89); C543(1.03)  LDD1594  [27]
 LDCM0391  CL120 HEK-293T C529(0.91); C524(0.94); C543(1.03)  LDD1595  [27]
 LDCM0392  CL121 HEK-293T C529(0.87); C524(0.91); C543(0.99)  LDD1596  [27]
 LDCM0393  CL122 HEK-293T C529(0.85); C524(0.99); C543(0.96)  LDD1597  [27]
 LDCM0394  CL123 HEK-293T C529(0.98); C524(0.89); C543(0.91)  LDD1598  [27]
 LDCM0395  CL124 HEK-293T C529(0.94); C524(0.88); C543(1.07)  LDD1599  [27]
 LDCM0396  CL125 HEK-293T C529(0.96); C524(0.92); C543(0.99)  LDD1600  [27]
 LDCM0397  CL126 HEK-293T C529(0.88); C524(1.02); C543(0.88)  LDD1601  [27]
 LDCM0398  CL127 HEK-293T C529(0.95); C524(0.97); C543(0.99)  LDD1602  [27]
 LDCM0399  CL128 HEK-293T C529(1.05); C524(1.12); C543(1.00)  LDD1603  [27]
 LDCM0400  CL13 HEK-293T C529(0.89); C524(0.76); C543(0.84)  LDD1604  [27]
 LDCM0401  CL14 HEK-293T C529(1.00); C524(0.95); C543(1.11)  LDD1605  [27]
 LDCM0402  CL15 HEK-293T C529(0.94); C524(0.96); C543(0.93)  LDD1606  [27]
 LDCM0403  CL16 HEK-293T C529(1.16); C524(0.82); C543(1.09)  LDD1607  [27]
 LDCM0404  CL17 HEK-293T C529(0.80); C524(0.76); C543(0.91)  LDD1608  [27]
 LDCM0405  CL18 HEK-293T C529(1.02); C524(1.05); C543(1.08)  LDD1609  [27]
 LDCM0406  CL19 HEK-293T C529(0.85); C524(0.90); C543(1.09)  LDD1610  [27]
 LDCM0407  CL2 HEK-293T C529(0.92); C524(1.02); C543(0.98)  LDD1611  [27]
 LDCM0408  CL20 HEK-293T C529(1.06); C524(1.00); C543(0.99)  LDD1612  [27]
 LDCM0409  CL21 HEK-293T C529(0.82); C524(0.79); C543(0.92)  LDD1613  [27]
 LDCM0410  CL22 HEK-293T C529(1.05); C524(0.96); C543(1.06)  LDD1614  [27]
 LDCM0411  CL23 HEK-293T C529(0.91); C524(0.99); C543(1.10)  LDD1615  [27]
 LDCM0412  CL24 HEK-293T C529(1.08); C524(1.22); C543(1.10)  LDD1616  [27]
 LDCM0413  CL25 HEK-293T C529(0.91); C524(0.76); C543(0.89)  LDD1617  [27]
 LDCM0414  CL26 HEK-293T C529(0.97); C524(1.11); C543(1.06)  LDD1618  [27]
 LDCM0415  CL27 HEK-293T C529(0.95); C524(0.83); C543(1.05)  LDD1619  [27]
 LDCM0416  CL28 HEK-293T C529(1.03); C524(0.90); C543(1.06)  LDD1620  [27]
 LDCM0417  CL29 HEK-293T C529(0.83); C524(0.93); C543(1.06)  LDD1621  [27]
 LDCM0418  CL3 HEK-293T C529(0.89); C524(0.98); C543(0.97)  LDD1622  [27]
 LDCM0419  CL30 HEK-293T C529(1.03); C524(0.97); C543(1.06)  LDD1623  [27]
 LDCM0420  CL31 HEK-293T C529(0.88); C524(0.97); C543(1.06)  LDD1624  [27]
 LDCM0421  CL32 HEK-293T C529(0.99); C524(1.07); C543(1.07)  LDD1625  [27]
 LDCM0422  CL33 HEK-293T C529(0.90); C524(0.88); C543(0.90)  LDD1626  [27]
 LDCM0423  CL34 HEK-293T C529(1.31); C524(0.91); C543(1.01)  LDD1627  [27]
 LDCM0424  CL35 HEK-293T C529(0.95); C524(1.03); C543(1.06)  LDD1628  [27]
 LDCM0425  CL36 HEK-293T C529(0.94); C524(1.10); C543(1.05)  LDD1629  [27]
 LDCM0426  CL37 HEK-293T C529(1.06); C524(0.89); C543(0.98)  LDD1630  [27]
 LDCM0428  CL39 HEK-293T C529(1.01); C524(0.93); C543(1.07)  LDD1632  [27]
 LDCM0429  CL4 HEK-293T C529(0.97); C524(1.02); C543(0.92)  LDD1633  [27]
 LDCM0430  CL40 HEK-293T C529(1.09); C524(1.01); C543(1.04)  LDD1634  [27]
 LDCM0431  CL41 HEK-293T C529(0.88); C524(0.96); C543(1.00)  LDD1635  [27]
 LDCM0432  CL42 HEK-293T C529(1.06); C524(1.01); C543(1.01)  LDD1636  [27]
 LDCM0433  CL43 HEK-293T C529(0.89); C524(0.93); C543(1.07)  LDD1637  [27]
 LDCM0434  CL44 HEK-293T C529(1.07); C524(0.89); C543(1.02)  LDD1638  [27]
 LDCM0435  CL45 HEK-293T C529(0.92); C524(0.98); C543(0.97)  LDD1639  [27]
 LDCM0436  CL46 HEK-293T C529(0.96); C524(0.89); C543(0.99)  LDD1640  [27]
 LDCM0437  CL47 HEK-293T C529(0.91); C524(1.08); C543(1.02)  LDD1641  [27]
 LDCM0438  CL48 HEK-293T C529(1.10); C524(1.15); C543(1.11)  LDD1642  [27]
 LDCM0439  CL49 HEK-293T C529(1.15); C524(0.91); C543(0.96)  LDD1643  [27]
 LDCM0440  CL5 HEK-293T C529(1.07); C524(0.84); C543(1.01)  LDD1644  [27]
 LDCM0441  CL50 HEK-293T C529(0.91); C524(0.96); C543(1.01)  LDD1645  [27]
 LDCM0443  CL52 HEK-293T C529(1.02); C524(1.03); C543(1.05)  LDD1646  [27]
 LDCM0444  CL53 HEK-293T C529(0.99); C524(0.81); C543(0.98)  LDD1647  [27]
 LDCM0445  CL54 HEK-293T C529(0.95); C524(0.96); C543(0.94)  LDD1648  [27]
 LDCM0446  CL55 HEK-293T C529(0.81); C524(0.92); C543(1.06)  LDD1649  [27]
 LDCM0447  CL56 HEK-293T C529(1.02); C524(0.98); C543(1.01)  LDD1650  [27]
 LDCM0448  CL57 HEK-293T C529(0.89); C524(0.96); C543(0.92)  LDD1651  [27]
 LDCM0449  CL58 HEK-293T C529(1.07); C524(1.05); C543(1.06)  LDD1652  [27]
 LDCM0450  CL59 HEK-293T C529(0.88); C524(1.03); C543(1.01)  LDD1653  [27]
 LDCM0451  CL6 HEK-293T C529(0.90); C524(0.82); C543(0.99)  LDD1654  [27]
 LDCM0452  CL60 HEK-293T C529(1.15); C524(0.91); C543(1.12)  LDD1655  [27]
 LDCM0453  CL61 HEK-293T C529(1.05); C524(0.92); C543(1.04)  LDD1656  [27]
 LDCM0454  CL62 HEK-293T C529(0.95); C524(1.13); C543(1.05)  LDD1657  [27]
 LDCM0455  CL63 HEK-293T C529(0.93); C524(0.79); C543(1.07)  LDD1658  [27]
 LDCM0456  CL64 HEK-293T C529(0.90); C524(1.16); C543(0.99)  LDD1659  [27]
 LDCM0457  CL65 HEK-293T C529(0.88); C524(0.97); C543(1.04)  LDD1660  [27]
 LDCM0458  CL66 HEK-293T C529(0.95); C524(1.00); C543(0.99)  LDD1661  [27]
 LDCM0459  CL67 HEK-293T C529(0.77); C524(0.85); C543(1.06)  LDD1662  [27]
 LDCM0460  CL68 HEK-293T C529(1.07); C524(1.11); C543(0.96)  LDD1663  [27]
 LDCM0461  CL69 HEK-293T C529(0.93); C524(1.06); C543(1.01)  LDD1664  [27]
 LDCM0462  CL7 HEK-293T C529(0.86); C524(0.90); C543(1.02)  LDD1665  [27]
 LDCM0463  CL70 HEK-293T C529(0.95); C524(1.03); C543(1.09)  LDD1666  [27]
 LDCM0464  CL71 HEK-293T C529(1.00); C524(1.12); C543(1.10)  LDD1667  [27]
 LDCM0465  CL72 HEK-293T C529(1.07); C524(1.10); C543(1.14)  LDD1668  [27]
 LDCM0466  CL73 HEK-293T C529(1.31); C524(0.88); C543(0.95)  LDD1669  [27]
 LDCM0467  CL74 HEK-293T C529(0.94); C524(1.03); C543(1.11)  LDD1670  [27]
 LDCM0469  CL76 HEK-293T C529(0.92); C524(1.01); C543(1.05)  LDD1672  [27]
 LDCM0470  CL77 HEK-293T C529(0.95); C524(0.85); C543(0.93)  LDD1673  [27]
 LDCM0471  CL78 HEK-293T C529(1.10); C524(0.83); C543(1.12)  LDD1674  [27]
 LDCM0472  CL79 HEK-293T C529(1.01); C524(1.04); C543(1.11)  LDD1675  [27]
 LDCM0473  CL8 HEK-293T C529(0.69); C524(0.71); C543(0.74)  LDD1676  [27]
 LDCM0474  CL80 HEK-293T C529(0.97); C524(0.98); C543(1.04)  LDD1677  [27]
 LDCM0475  CL81 HEK-293T C529(0.96); C524(1.05); C543(1.09)  LDD1678  [27]
 LDCM0476  CL82 HEK-293T C529(1.03); C524(1.00); C543(1.05)  LDD1679  [27]
 LDCM0477  CL83 HEK-293T C529(0.98); C524(0.96); C543(1.04)  LDD1680  [27]
 LDCM0478  CL84 HEK-293T C529(1.17); C524(1.33); C543(1.07)  LDD1681  [27]
 LDCM0479  CL85 HEK-293T C529(1.10); C524(0.96); C543(1.05)  LDD1682  [27]
 LDCM0480  CL86 HEK-293T C529(0.79); C524(0.90); C543(0.92)  LDD1683  [27]
 LDCM0481  CL87 HEK-293T C529(0.98); C524(0.90); C543(1.03)  LDD1684  [27]
 LDCM0482  CL88 HEK-293T C529(0.93); C524(0.94); C543(0.98)  LDD1685  [27]
 LDCM0483  CL89 HEK-293T C529(1.00); C524(0.89); C543(1.08)  LDD1686  [27]
 LDCM0484  CL9 HEK-293T C529(1.03); C524(0.81); C543(0.93)  LDD1687  [27]
 LDCM0485  CL90 HEK-293T C529(0.80); C524(0.74); C543(0.77)  LDD1688  [27]
 LDCM0486  CL91 HEK-293T C529(0.86); C524(0.82); C543(1.04)  LDD1689  [27]
 LDCM0487  CL92 HEK-293T C529(1.03); C524(0.90); C543(0.90)  LDD1690  [27]
 LDCM0488  CL93 HEK-293T C529(0.84); C524(0.99); C543(0.93)  LDD1691  [27]
 LDCM0489  CL94 HEK-293T C529(0.84); C524(0.92); C543(0.97)  LDD1692  [27]
 LDCM0490  CL95 HEK-293T C529(0.79); C524(0.85); C543(0.83)  LDD1693  [27]
 LDCM0491  CL96 HEK-293T C529(1.02); C524(0.93); C543(0.98)  LDD1694  [27]
 LDCM0492  CL97 HEK-293T C529(0.88); C524(0.81); C543(0.89)  LDD1695  [27]
 LDCM0493  CL98 HEK-293T C529(0.82); C524(0.98); C543(0.87)  LDD1696  [27]
 LDCM0494  CL99 HEK-293T C529(0.94); C524(0.92); C543(0.97)  LDD1697  [27]
 LDCM0495  E2913 HEK-293T C529(1.11); C524(0.95); C543(1.01)  LDD1698  [27]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C384(0.95)  LDD1702  [5]
 LDCM0574  Fragment12 Ramos 0.82  LDD2191  [28]
 LDCM0575  Fragment13 Ramos 1.04  LDD2192  [28]
 LDCM0580  Fragment21 Ramos 0.99  LDD2195  [28]
 LDCM0578  Fragment27 Ramos 1.36  LDD2197  [28]
 LDCM0586  Fragment28 Ramos 1.23  LDD2198  [28]
 LDCM0588  Fragment30 Ramos 0.97  LDD2199  [28]
 LDCM0589  Fragment31 Ramos 0.79  LDD2200  [28]
 LDCM0590  Fragment32 Ramos 1.19  LDD2201  [28]
 LDCM0468  Fragment33 HEK-293T C529(1.09); C524(0.87); C543(1.06)  LDD1671  [27]
 LDCM0596  Fragment38 Ramos 0.77  LDD2203  [28]
 LDCM0427  Fragment51 HEK-293T C529(0.92); C524(1.03); C543(0.94)  LDD1631  [27]
 LDCM0116  HHS-0101 DM93 Y278(0.92); Y401(0.93)  LDD0264  [13]
 LDCM0117  HHS-0201 DM93 Y278(0.94); Y401(1.03)  LDD0265  [13]
 LDCM0118  HHS-0301 DM93 Y278(1.04); Y401(1.06)  LDD0266  [13]
 LDCM0119  HHS-0401 DM93 Y278(1.42); Y401(1.59)  LDD0267  [13]
 LDCM0120  HHS-0701 DM93 Y278(1.95); Y401(4.22)  LDD0268  [13]
 LDCM0107  IAA HeLa N.A.  LDD0221  [23]
 LDCM0123  JWB131 DM93 Y278(0.88)  LDD0285  [12]
 LDCM0124  JWB142 DM93 Y278(0.57)  LDD0286  [12]
 LDCM0125  JWB146 DM93 Y278(1.36)  LDD0287  [12]
 LDCM0126  JWB150 DM93 Y278(6.30)  LDD0288  [12]
 LDCM0127  JWB152 DM93 Y278(1.67)  LDD0289  [12]
 LDCM0128  JWB198 DM93 Y278(1.14)  LDD0290  [12]
 LDCM0129  JWB202 DM93 Y278(0.22)  LDD0291  [12]
 LDCM0130  JWB211 DM93 Y278(1.14)  LDD0292  [12]
 LDCM0022  KB02 HEK-293T C524(1.10); C529(1.00); C543(0.94)  LDD1492  [27]
 LDCM0023  KB03 HEK-293T C524(1.15); C529(0.90); C543(1.01)  LDD1497  [27]
 LDCM0024  KB05 SKMEL3 C389(1.92)  LDD3324  [10]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C384(0.67)  LDD2121  [5]
 LDCM0109  NEM HeLa N.A.  LDD0223  [23]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C384(0.71)  LDD2089  [5]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C384(1.83)  LDD2094  [5]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C543(0.72)  LDD2097  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C384(1.12); C543(0.72)  LDD2099  [5]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C543(0.31)  LDD2100  [5]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C384(1.00); C543(0.81); C540(1.06)  LDD2101  [5]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C540(0.32)  LDD2104  [5]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C384(1.26)  LDD2105  [5]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C384(0.48); C543(0.16)  LDD2106  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C543(0.58); C540(0.59)  LDD2107  [5]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C384(1.13); C543(0.33); C540(0.48)  LDD2108  [5]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C543(0.54); C540(0.40)  LDD2109  [5]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C384(0.47); C543(0.34)  LDD2110  [5]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C384(1.25); C543(0.66); C540(0.54)  LDD2111  [5]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C384(0.50)  LDD2114  [5]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C543(0.36); C540(0.54)  LDD2115  [5]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C384(1.97)  LDD2119  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C543(0.54)  LDD2123  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C384(1.00)  LDD2124  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C384(0.74); C543(0.78); C540(0.58)  LDD2125  [5]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C384(1.05)  LDD2126  [5]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C384(0.85); C543(0.59); C540(0.59)  LDD2127  [5]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C384(1.11)  LDD2129  [5]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C384(0.57); C543(0.46); C540(0.46)  LDD2133  [5]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C384(0.48); C543(0.39)  LDD2134  [5]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C384(0.74); C543(0.68); C540(0.65)  LDD2136  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C543(0.63); C540(1.20)  LDD2137  [5]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C543(1.20)  LDD1700  [5]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C384(0.71); C543(0.75)  LDD2140  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C384(0.52); C543(0.79); C540(0.99)  LDD2141  [5]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C384(0.95)  LDD2144  [5]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C384(1.07); C543(0.85); C540(0.68)  LDD2145  [5]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C543(0.64)  LDD2146  [5]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C384(0.38); C543(0.38)  LDD2148  [5]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C384(0.52)  LDD2150  [5]
 LDCM0131  RA190 MM1.R C524(1.17); C529(1.17)  LDD0304  [29]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein arginine N-methyltransferase 1 (PRMT1) Protein arginine N-methyltransferase family Q99873
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Phospholipid scramblase 1 (PLSCR1) Phospholipid scramblase family O15162
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Ataxin-3 (ATXN3) . P54252
Histone acetyltransferase p300 (EP300) . Q09472
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Wolframin (WFS1) . O76024
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 4 (SMAD4) Dwarfin/SMAD family Q13485
Zinc finger protein 165 (ZNF165) Krueppel C2H2-type zinc-finger protein family P49910
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) ATP12 family Q8N5M1
MAPK-interacting and spindle-stabilizing protein-like (MAPK1IP1L) MISS family Q8NDC0
RNA-binding protein EWS (EWSR1) RRM TET family Q01844
RNA-binding protein FUS (FUS) RRM TET family P35637
Protein transport protein Sec24A (SEC24A) SEC23/SEC24 family O95486
Protein transport protein Sec24D (SEC24D) SEC23/SEC24 family O94855
Peflin (PEF1) . Q9UBV8
Proline-rich protein 13 (PRR13) . Q9NZ81
Protein TFG (TFG) . Q92734
Single-stranded DNA-binding protein 3 (SSBP3) . Q9BWW4
TNF receptor-associated factor 1 (TRAF1) . Q13077

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
7 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
8 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
9 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
11 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
12 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
13 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
15 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
16 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
17 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
18 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
19 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
20 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
21 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
22 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
23 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
24 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
25 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
26 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094
27 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
28 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
29 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.