Details of the Probe

General Information of Probe

Probe ID
LDPC0433
Probe Name
C197
Synonyms
Z4282860310
Probe Type
PAL-AfBPP Probe
Structure
2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 350.5
Lipid-water partition coefficient (xlogp) 2.5
Rotatable Bond Count (rotbonds) 6
Hydrogen Bond Donor Count (hbonddonor) 0
Hydrogen Bond Acceptor Count (hbondacc) 4
Chemical Identifiers
Formula
C21H26N4O
IUPAC Name
3-(3-but-3-ynyldiazirin-3-yl)-1-(2-methyl-3-phenyl-1,3,3a,4,6,6a-hexahydropyrrolo[3,4-c]pyrrol-5-yl)propan-1-one
Canonical SMILES
CN1CC2CN(CC2C1C3=CC=CC=C3)C(=O)CCC4(N=N4)CCC#C
InChI
InChI=1S/C21H26N4O/c1-3-4-11-21(22-23-21)12-10-19(26)25-14-17-13-24(2)20(18(17)15-25)16-8-6-5-7-9-16/h1,5-9,17-18,20H,4,10-15H2,2H3
InChIKey
IGPYVVOODXVYCI-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

76 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-ketodihydrosphingosine reductase (KDSR) LDTP05476 6.82  LDD1873 
5'-3' exonuclease PLD3 (PLD3) LDTP08476 9.38  LDD1873 
7-dehydrocholesterol reductase (DHCR7) LDTP13131 9.32  LDD1873 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 5.58  LDD1873 
Acid ceramidase (ASAH1) LDTP05905 6.23  LDD1873 
Adenylosuccinate synthetase isozyme 2 (ADSS2) LDTP03541 9.06  LDD1873 
Alpha-galactosidase A (GLA) LDTP02141 17.51  LDD1873 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 9.78  LDD1873 
ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) LDTP06618 8.34  LDD1873 
Beta-1,4-glucuronyltransferase 1 (B4GAT1) LDTP00783 7.36  LDD1873 
Beta-galactosidase (GLB1) LDTP02837 11.55  LDD1873 
Beta-hexosaminidase subunit alpha (HEXA) LDTP02169 12.30  LDD1873 
Beta-hexosaminidase subunit beta (HEXB) LDTP02216 6.77  LDD1873 
Beta-mannosidase (MANBA) LDTP00282 11.00  LDD1873 
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 20.68  LDD1873 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 9.85  LDD1873 
Carboxypeptidase D (CPD) LDTP01372 12.21  LDD1873 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 7.31  LDD1873 
Cathepsin B (CTSB) LDTP02223 19.84  LDD1873 
CCR4-NOT transcription complex subunit 4 (CNOT4) LDTP01640 5.10  LDD1873 
Ceramide glucosyltransferase (UGCG) LDTP06617 9.92  LDD1873 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 5.82  LDD1873 
Cytochrome b5 type B (CYB5B) LDTP00697 10.48  LDD1873 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 21.86  LDD1873 
Delta(24)-sterol reductase (DHCR24) LDTP06375 8.17  LDD1873 
Deoxyribonuclease-2-alpha (DNASE2) LDTP00185 12.73  LDD1873 
Di-N-acetylchitobiase (CTBS) LDTP14801 5.39  LDD1873 
Dipeptidyl peptidase 1 (CTSC) LDTP04572 7.84  LDD1873 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 14.32  LDD1873 
Ephrin type-A receptor 7 (EPHA7) LDTP06370 6.11  LDD1873 
Estradiol 17-beta-dehydrogenase 11 (HSD17B11) LDTP09063 5.62  LDD1873 
Exonuclease 1 (EXO1) LDTP13751 5.31  LDD1873 
Gamma-glutamyl hydrolase (GGH) LDTP09886 8.00  LDD1873 
Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) LDTP12732 22.16  LDD1873 
Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) LDTP01333 5.46  LDD1873 
Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) LDTP04211 6.32  LDD1873 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 15.89  LDD1873 
Lathosterol oxidase (SC5D) LDTP01329 4.92  LDD1873 
Legumain (LGMN) LDTP10888 15.78  LDD1873 
Leucyl-cystinyl aminopeptidase (LNPEP) LDTP13358 8.46  LDD1873 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 12.91  LDD1873 
Long-chain fatty acid transport protein 2 (SLC27A2) LDTP00496 5.03  LDD1873 
Lysosomal acid phosphatase (ACP2) LDTP02539 10.34  LDD1873 
Lysosomal alpha-glucosidase (GAA) LDTP02486 7.21  LDD1873 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 7.94  LDD1873 
Lysosomal protective protein (CTSA) LDTP02505 8.51  LDD1873 
Lysosomal thioesterase PPT2 (PPT2) LDTP13620 5.78  LDD1873 
N-acetylglucosamine-6-sulfatase (GNS) LDTP02799 15.67  LDD1873 
N-alpha-acetyltransferase 40 (NAA40) LDTP08265 6.23  LDD1873 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 7.06  LDD1873 
Nicotinamide phosphoribosyltransferase (NAMPT) LDTP04051 11.71  LDD1873 
Obg-like ATPase 1 (OLA1) LDTP12610 6.19  LDD1873 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 17.63  LDD1873 
Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) LDTP12628 12.47  LDD1873 
Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) LDTP12720 4.96  LDD1873 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 6.73  LDD1873 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 6.87  LDD1873 
Phospholipase A2 group XV (PLA2G15) LDTP09088 8.46  LDD1873 
Plasma alpha-L-fucosidase (FUCA2) LDTP11215 13.93  LDD1873 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 8.75  LDD1873 
Presequence protease, mitochondrial (PITRM1) LDTP07003 5.66  LDD1873 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 7.84  LDD1873 
Prostaglandin reductase 3 (PTGR3) LDTP15422 9.51  LDD1873 
Proteasome subunit beta type-1 (PSMB1) LDTP03060 5.10  LDD1873 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 9.65  LDD1873 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 7.73  LDD1873 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 8.17  LDD1873 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 26.91  LDD1873 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 18.90  LDD1873 
Ribonuclease T2 (RNASET2) LDTP00316 18.25  LDD1873 
Signal peptide peptidase-like 2A (SPPL2A) LDTP09395 13.55  LDD1873 
Sulfhydryl oxidase 2 (QSOX2) LDTP07813 6.06  LDD1873 
Tissue alpha-L-fucosidase (FUCA1) LDTP02006 7.84  LDD1873 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 9.25  LDD1873 
Ubiquitin thioesterase OTUB1 (OTUB1) LDTP10318 10.70  LDD1873 
V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) LDTP14030 7.21  LDD1873 
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⏷ Show the Full List of 76 Enzyme
51 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) LDTP09328 6.23  LDD1873 
Amyloid-beta precursor protein (APP) LDTP02071 16.22  LDD1873 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 9.06  LDD1873 
Cation-independent mannose-6-phosphate receptor (IGF2R) LDTP02586 12.13  LDD1873 
CMP-sialic acid transporter (SLC35A1) LDTP05116 8.22  LDD1873 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 7.16  LDD1873 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 8.46  LDD1873 
H(+)/Cl(-) exchange transporter 3 (CLCN3) LDTP04459 10.56  LDD1873 
Integral membrane protein 2C (ITM2C) LDTP12451 12.04  LDD1873 
Lysosome membrane protein 2 (SCARB2) LDTP06034 13.45  LDD1873 
Lysosome-associated membrane glycoprotein 1 (LAMP1) LDTP02562 7.21  LDD1873 
Magnesium transporter NIPA3 (NIPAL1) LDTP17362 10.70  LDD1873 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 6.87  LDD1873 
Metaxin-1 (MTX1) LDTP05900 5.31  LDD1873 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 25.99  LDD1873 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 11.47  LDD1873 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 5.35  LDD1873 
Mpv17-like protein 2 (MPV17L2) LDTP14952 9.78  LDD1873 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 6.63  LDD1873 
NPC intracellular cholesterol transporter 2 (NPC2) LDTP04940 11.00  LDD1873 
Occludin (OCLN) LDTP06581 7.89  LDD1873 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 6.73  LDD1873 
Prosaposin (PSAP) LDTP02215 16.68  LDD1873 
Protein FAM8A1 (FAM8A1) LDTP16190 5.35  LDD1873 
Protein GPR107 (GPR107) LDTP07236 11.71  LDD1873 
Protein GPR108 (GPR108) LDTP12383 9.13  LDD1873 
Protein tweety homolog 3 (TTYH3) LDTP11615 15.03  LDD1873 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 8.69  LDD1873 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 8.88  LDD1873 
Slit homolog 2 protein (SLIT2) LDTP01427 6.11  LDD1873 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 8.88  LDD1873 
Solute carrier family 25 member 44 (SLC25A44) LDTP15733 7.73  LDD1873 
Solute carrier family 35 member F1 (SLC35F1) LDTP15000 7.06  LDD1873 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 5.98  LDD1873 
Sortilin (SORT1) LDTP10885 7.36  LDD1873 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 6.59  LDD1873 
Stomatin (STOM) LDTP03380 10.56  LDD1873 
Surfeit locus protein 4 (SURF4) LDTP00592 5.46  LDD1873 
Tetraspanin-3 (TSPAN3) LDTP16813 15.14  LDD1873 
Transferrin receptor protein 1 (TFRC) LDTP01969 7.11  LDD1873 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 7.84  LDD1873 
Translocator protein (TSPO) LDTP03546 5.82  LDD1873 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 5.31  LDD1873 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 6.28  LDD1873 
Transmembrane protein 126A (TMEM126A) LDTP15948 10.93  LDD1873 
Transmembrane protein 160 (TMEM160) LDTP20002 9.00  LDD1873 
Transmembrane protein 199 (TMEM199) LDTP08881 7.11  LDD1873 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 9.65  LDD1873 
V-type proton ATPase subunit S1 (ATP6AP1) LDTP06499 6.41  LDD1873 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 15.56  LDD1873 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 11.08  LDD1873 
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⏷ Show the Full List of 51 Transporter and channel
3 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
HLA class I histocompatibility antigen, B alpha chain (HLA-B) LDTP01913 5.10  LDD1873 
HLA class I histocompatibility antigen, C alpha chain (HLA-C) LDTP02491 7.78  LDD1873 
IgG receptor FcRn large subunit p51 (FCGRT) LDTP04720 7.57  LDD1873 
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37 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Amyloid beta precursor like protein 2 (APLP2) LDTP05491 8.82  LDD1873 
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 6.77  LDD1873 
Apolipoprotein L2 (APOL2) LDTP11076 17.51  LDD1873 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 7.16  LDD1873 
Calumenin (CALU) LDTP00887 5.46  LDD1873 
Clustered mitochondria protein homolog (CLUH) LDTP14486 9.99  LDD1873 
Collectin-12 (COLEC12) LDTP07031 7.57  LDD1873 
Cytoplasmic tRNA 2-thiolation protein 2 (CTU2) LDTP06735 5.10  LDD1873 
Desmocollin-3 (DSC3) LDTP06154 7.46  LDD1873 
Desmoglein-2 (DSG2) LDTP06042 5.10  LDD1873 
Dyslexia-associated protein KIAA0319-like protein (KIAA0319L) LDTP08689 15.14  LDD1873 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 6.02  LDD1873 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 4.96  LDD1873 
Fragile X messenger ribonucleoprotein 1 (FMR1) LDTP05501 5.17  LDD1873 
Galectin-3-binding protein (LGALS3BP) LDTP05558 12.30  LDD1873 
Ganglioside GM2 activator (GM2A) LDTP02927 7.41  LDD1873 
Golgi apparatus protein 1 (GLG1) LDTP09920 8.46  LDD1873 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 9.06  LDD1873 
Keratinocyte-associated transmembrane protein 2 (KCT2) LDTP19780 11.55  LDD1873 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 23.10  LDD1873 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 8.57  LDD1873 
Negative elongation factor C/D (NELFCD) LDTP08594 4.96  LDD1873 
Nicastrin (NCSTN) LDTP09787 6.54  LDD1873 
Nucleobindin-1 (NUCB1) LDTP05375 6.50  LDD1873 
Nucleobindin-2 (NUCB2) LDTP05160 17.39  LDD1873 
Pre-mRNA-splicing factor 38B (PRPF38B) LDTP17266 4.96  LDD1873 
Protein SYS1 homolog (SYS1) LDTP08796 10.34  LDD1873 
Protein unc-119 homolog B (UNC119B) LDTP00105 6.50  LDD1873 
Reticulocalbin-2 (RCN2) LDTP06097 5.54  LDD1873 
RNA-binding protein EWS (EWSR1) LDTP05318 5.43  LDD1873 
RNA-binding protein FUS (FUS) LDTP03775 5.31  LDD1873 
Sequestosome-1 (SQSTM1) LDTP05898 5.50  LDD1873 
Small ribosomal subunit protein mS33 (MRPS33) LDTP18561 6.59  LDD1873 
Tetraspanin-6 (TSPAN6) LDTP14458 17.88  LDD1873 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 5.24  LDD1873 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 7.67  LDD1873 
Zinc finger CCHC domain-containing protein 3 (ZCCHC3) LDTP12623 6.59  LDD1873 
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⏷ Show the Full List of 37 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1873 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587