Details of the Target

General Information of Target

Target ID LDTP00316
Target Name Ribonuclease T2 (RNASET2)
Gene Name RNASET2
Gene ID 8635
Synonyms
RNASE6PL; Ribonuclease T2; EC 4.6.1.19; Ribonuclease 6
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRPAALRGALLGCLCLALLCLGGADKRLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPD
YWTIHGLWPDKSEGCNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGT
CAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYYQVADFKDALARVYGVIP
KIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGAAESRGLRV
CEDGPVFYPPPKKTKH
Target Bioclass
Enzyme
Family
RNase T2 family
Subcellular location
Secreted
Function
Ribonuclease that plays an essential role in innate immune response by recognizing and degrading RNAs from microbial pathogens that are subsequently sensed by TLR8. Cleaves preferentially single-stranded RNA molecules between purine and uridine residues, which critically contributes to the supply of catabolic uridine and the generation of purine-2',3'-cyclophosphate-terminated oligoribonucleotides. In turn, RNase T2 degradation products promote the RNA-dependent activation of TLR8. Also plays a key role in degradation of mitochondrial RNA and processing of non-coding RNA imported from the cytosol into mitochondria. Participates as well in degradation of mitochondrion-associated cytosolic rRNAs.
Uniprot ID
O00584
Ensemble ID
ENST00000421787.5
HGNC ID
HGNC:21686

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.E140K .
NCIH2286 SNV: p.R174G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		4.89  LDD0066  [1]
Alkylaryl probe 1
 Probe Info  		3.00  LDD0386  [2]
FBPP2
 Probe Info  		3.61  LDD0054  [3]
DBIA
 Probe Info  		C48(1.04)  LDD1492  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
NAIA_5
 Probe Info  		C121(0.00); C48(0.00); C241(0.00)  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 40 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C036
 Probe Info  		5.62  LDD1736  [8]
C041
 Probe Info  		6.28  LDD1741  [8]
C045
 Probe Info  		12.73  LDD1744  [8]
C087
 Probe Info  		8.34  LDD1779  [8]
C106
 Probe Info  		24.08  LDD1793  [8]
C108
 Probe Info  		8.11  LDD1795  [8]
C115
 Probe Info  		12.73  LDD1802  [8]
C149
 Probe Info  		13.64  LDD1830  [8]
C164
 Probe Info  		10.70  LDD1844  [8]
C170
 Probe Info  		42.81  LDD1850  [8]
C171
 Probe Info  		10.56  LDD1851  [8]
C177
 Probe Info  		8.06  LDD1856  [8]
C184
 Probe Info  		7.73  LDD1862  [8]
C186
 Probe Info  		20.97  LDD1864  [8]
C187
 Probe Info  		16.34  LDD1865  [8]
C197
 Probe Info  		18.25  LDD1873  [8]
C208
 Probe Info  		19.03  LDD1883  [8]
C211
 Probe Info  		9.85  LDD1885  [8]
C221
 Probe Info  		16.34  LDD1895  [8]
C223
 Probe Info  		16.11  LDD1897  [8]
C232
 Probe Info  		33.36  LDD1905  [8]
C237
 Probe Info  		12.21  LDD1910  [8]
C281
 Probe Info  		10.85  LDD1951  [8]
C302
 Probe Info  		12.38  LDD1971  [8]
C310
 Probe Info  		28.05  LDD1977  [8]
C314
 Probe Info  		13.27  LDD1981  [8]
C326
 Probe Info  		7.84  LDD1990  [8]
C334
 Probe Info  		8.34  LDD1997  [8]
C336
 Probe Info  		6.28  LDD1999  [8]
C346
 Probe Info  		15.89  LDD2007  [8]
C383
 Probe Info  		11.39  LDD2042  [8]
C386
 Probe Info  		7.16  LDD2045  [8]
C390
 Probe Info  		24.25  LDD2049  [8]
C413
 Probe Info  		14.52  LDD2069  [8]
C420
 Probe Info  		14.62  LDD2075  [8]
C425
 Probe Info  		25.11  LDD2080  [8]
C431
 Probe Info  		18.25  LDD2086  [8]
FFF probe12
 Probe Info  		20.00  LDD0473  [9]
FFF probe13
 Probe Info  		20.00  LDD0475  [9]
FFF probe9
 Probe Info  		20.00  LDD0470  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C48(0.92); C184(1.18)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C48(1.03); C55(1.02)  LDD1509  [4]
 LDCM0237  AC12 HEK-293T C48(0.99); C55(0.79)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C48(1.02)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C48(1.00); C55(1.00)  LDD1513  [4]
 LDCM0277  AC18 HEK-293T C48(1.15); C184(1.19)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C48(1.32); C55(1.11)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C48(0.83); C184(0.92)  LDD1518  [4]
 LDCM0280  AC20 HEK-293T C48(1.22); C55(0.99)  LDD1519  [4]
 LDCM0281  AC21 HEK-293T C48(1.04); C55(1.00)  LDD1520  [4]
 LDCM0282  AC22 HEK-293T C48(1.05)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C48(1.15); C55(1.02)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C48(1.01); C55(1.06); C121(1.03)  LDD1523  [4]
 LDCM0286  AC26 HEK-293T C48(0.95); C184(1.17)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C48(0.97); C55(1.00)  LDD1526  [4]
 LDCM0288  AC28 HEK-293T C48(1.17); C55(0.91)  LDD1527  [4]
 LDCM0289  AC29 HEK-293T C48(1.03); C55(0.91)  LDD1528  [4]
 LDCM0290  AC3 HEK-293T C48(0.95); C55(1.01)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C48(0.95)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C48(1.02); C55(1.23)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C48(0.96); C55(1.24); C121(1.07)  LDD1532  [4]
 LDCM0295  AC34 HEK-293T C48(1.04); C184(1.06)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C48(1.10); C55(0.79)  LDD1535  [4]
 LDCM0297  AC36 HEK-293T C48(1.07); C55(0.92)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C48(0.92); C55(0.91)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C48(0.96)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C48(1.08); C55(0.86)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C48(1.04); C55(0.76)  LDD1540  [4]
 LDCM0302  AC40 HEK-293T C48(0.88); C55(1.12); C121(1.10)  LDD1541  [4]
 LDCM0304  AC42 HEK-293T C48(1.00); C184(1.16)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C48(1.08); C55(0.85)  LDD1544  [4]
 LDCM0306  AC44 HEK-293T C48(1.12); C55(0.81)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C48(0.98); C55(1.02)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C48(1.03)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C48(1.00); C55(1.15)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C48(1.08); C55(0.98); C121(1.01)  LDD1549  [4]
 LDCM0312  AC5 HEK-293T C48(0.98); C55(1.02)  LDD1551  [4]
 LDCM0313  AC50 HEK-293T C48(0.99); C184(0.99)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C48(1.16); C55(1.00)  LDD1553  [4]
 LDCM0315  AC52 HEK-293T C48(1.13); C55(1.19)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C48(1.07); C55(1.13)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C48(0.99)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C48(1.00); C55(1.05)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C48(1.02); C55(1.15); C121(1.02)  LDD1558  [4]
 LDCM0321  AC58 HEK-293T C48(0.97); C184(1.03)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C48(0.99); C55(0.89)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C48(1.06)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C48(0.98); C55(1.03)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C48(1.02); C55(1.13)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C48(1.01)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C48(1.06); C55(1.43)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C48(1.09); C55(1.31); C121(1.04)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C48(1.06); C55(0.90)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C48(0.97); C55(0.96); C121(1.01)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C48(0.98); C55(1.05)  LDD1511  [4]
 LDCM0275  AKOS034007705 HEK-293T C48(1.03); C55(0.97); C121(1.00)  LDD1514  [4]
 LDCM0367  CL1 HEK-293T C48(1.27); C55(0.88)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C48(1.37)  LDD1572  [4]
 LDCM0370  CL101 HEK-293T C48(0.98); C55(1.09)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C48(1.01)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C48(0.95); C55(0.94)  LDD1576  [4]
 LDCM0374  CL105 HEK-293T C48(0.99); C55(1.23)  LDD1578  [4]
 LDCM0375  CL106 HEK-293T C48(1.10)  LDD1579  [4]
 LDCM0376  CL107 HEK-293T C48(1.08); C55(0.88)  LDD1580  [4]
 LDCM0378  CL109 HEK-293T C48(1.04); C55(1.02)  LDD1582  [4]
 LDCM0379  CL11 HEK-293T C48(1.22); C55(0.59)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C48(1.12)  LDD1584  [4]
 LDCM0381  CL111 HEK-293T C48(1.10); C55(0.99)  LDD1585  [4]
 LDCM0383  CL113 HEK-293T C48(1.17); C55(0.83)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C48(1.08)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C48(1.00); C55(0.94)  LDD1589  [4]
 LDCM0387  CL117 HEK-293T C48(1.15); C55(1.06)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C48(1.20)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C48(1.08); C55(0.90)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C48(1.04); C55(0.90); C121(0.95)  LDD1594  [4]
 LDCM0392  CL121 HEK-293T C48(1.09); C55(1.11)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C48(1.02)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C48(0.98); C55(1.10)  LDD1598  [4]
 LDCM0396  CL125 HEK-293T C48(1.12); C55(1.49)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C48(0.89)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C48(1.03); C55(1.21)  LDD1602  [4]
 LDCM0400  CL13 HEK-293T C48(1.08); C55(1.04)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C48(0.98)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C48(1.13); C55(0.89)  LDD1606  [4]
 LDCM0405  CL18 HEK-293T C48(1.04); C184(0.97)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C48(0.97); C55(0.77)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C48(0.92)  LDD1611  [4]
 LDCM0408  CL20 HEK-293T C48(1.04); C55(0.59)  LDD1612  [4]
 LDCM0409  CL21 HEK-293T C48(1.02); C55(0.68)  LDD1613  [4]
 LDCM0410  CL22 HEK-293T C48(1.07)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C48(1.27); C55(0.62)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C48(1.11); C55(0.72); C121(1.02)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C48(1.19); C55(0.94)  LDD1617  [4]
 LDCM0414  CL26 HEK-293T C48(0.92)  LDD1618  [4]
 LDCM0415  CL27 HEK-293T C48(1.02); C55(0.89)  LDD1619  [4]
 LDCM0418  CL3 HEK-293T C48(1.13); C55(0.92)  LDD1622  [4]
 LDCM0419  CL30 HEK-293T C48(0.85); C184(1.02)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C48(1.08); C55(0.67)  LDD1624  [4]
 LDCM0421  CL32 HEK-293T C48(1.23); C55(0.63)  LDD1625  [4]
 LDCM0422  CL33 HEK-293T C48(1.08); C55(0.62)  LDD1626  [4]
 LDCM0423  CL34 HEK-293T C48(1.18)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C48(1.07); C55(0.58)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C48(1.08); C55(0.67); C121(0.97)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C48(1.02); C55(1.19)  LDD1630  [4]
 LDCM0428  CL39 HEK-293T C48(0.97); C55(0.93)  LDD1632  [4]
 LDCM0432  CL42 HEK-293T C48(0.96); C184(0.88)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C48(0.99); C55(0.79)  LDD1637  [4]
 LDCM0434  CL44 HEK-293T C48(1.03); C55(1.02)  LDD1638  [4]
 LDCM0435  CL45 HEK-293T C48(0.98); C55(0.62)  LDD1639  [4]
 LDCM0436  CL46 HEK-293T C48(1.00)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C48(1.19); C55(0.59)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C48(0.96); C55(0.71); C121(0.95)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C48(1.15); C55(1.24)  LDD1643  [4]
 LDCM0441  CL50 HEK-293T C48(0.85)  LDD1645  [4]
 LDCM0445  CL54 HEK-293T C48(0.94); C184(1.21)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C48(0.98); C55(0.65)  LDD1649  [4]
 LDCM0447  CL56 HEK-293T C48(1.11); C55(0.62)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C48(0.96); C55(0.76)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C48(1.01)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C48(1.14); C55(0.67)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C48(0.96); C184(0.95)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C48(0.98); C55(0.78); C121(0.96)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C48(1.04); C55(1.34)  LDD1656  [4]
 LDCM0454  CL62 HEK-293T C48(0.91)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C48(1.04); C55(0.93)  LDD1658  [4]
 LDCM0458  CL66 HEK-293T C48(0.79); C184(1.08)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C48(0.95); C55(0.84)  LDD1662  [4]
 LDCM0460  CL68 HEK-293T C48(1.11); C55(0.52)  LDD1663  [4]
 LDCM0461  CL69 HEK-293T C48(0.99); C55(0.73)  LDD1664  [4]
 LDCM0462  CL7 HEK-293T C48(1.18); C55(0.63)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C48(1.19)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C48(1.12); C55(0.73)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C48(1.04); C55(0.72); C121(1.10)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C48(1.15); C55(1.14)  LDD1669  [4]
 LDCM0467  CL74 HEK-293T C48(0.97)  LDD1670  [4]
 LDCM0471  CL78 HEK-293T C48(0.93); C184(0.97)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C48(0.99); C55(0.65)  LDD1675  [4]
 LDCM0473  CL8 HEK-293T C48(1.30); C55(0.65)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C48(1.00); C55(0.67)  LDD1677  [4]
 LDCM0475  CL81 HEK-293T C48(1.05); C55(0.64)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C48(1.12)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C48(1.18); C55(0.76)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C48(0.99); C55(0.95); C121(0.95)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C48(0.96); C55(1.46)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C48(0.98)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C48(0.98); C55(1.32)  LDD1684  [4]
 LDCM0484  CL9 HEK-293T C48(1.01); C55(0.68)  LDD1687  [4]
 LDCM0485  CL90 HEK-293T C48(0.99); C184(1.01)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C48(1.06); C55(0.88)  LDD1689  [4]
 LDCM0487  CL92 HEK-293T C48(1.04); C55(1.02)  LDD1690  [4]
 LDCM0488  CL93 HEK-293T C48(1.00); C55(0.72)  LDD1691  [4]
 LDCM0489  CL94 HEK-293T C48(1.01)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C48(1.16); C55(0.80)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C48(0.84); C55(0.92); C121(0.95)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C48(0.97); C55(0.94)  LDD1695  [4]
 LDCM0493  CL98 HEK-293T C48(1.05)  LDD1696  [4]
 LDCM0494  CL99 HEK-293T C48(1.00); C55(1.54)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C48(1.00); C55(0.97)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C48(1.03); C55(1.03)  LDD1671  [4]
 LDCM0427  Fragment51 HEK-293T C48(0.97)  LDD1631  [4]
 LDCM0022  KB02 HEK-293T C48(1.04)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C48(1.00)  LDD1497  [4]
 LDCM0024  KB05 HEK-293T C48(0.97)  LDD1502  [4]
 LDCM0099  Phenelzine HEK-293T 3.00  LDD0386  [2]
 LDCM0008  Tranylcypromine SH-SY5Y 3.61  LDD0054  [3]

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.