Details of the Probe

General Information of Probe

Probe ID
LDPC0202
Probe Name
Alkylaryl probe 1
Synonyms
(Prop-2-yn-1-yl)hydrazine; 6068-67-3; prop-2-ynylhydrazine; PROP-2-YNYL-HYDRAZINE; 1-(2-propynyl)hydrazine; propargylhydrazine; N-(prop-2-yn-1-yl)-hydrazine; DTXSID20574430; CYCYZHMUOSKYBG-UHFFFAOYSA-N; AKOS006307124; EN300-261024
Probe Type
ABPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 70.09
Lipid-water partition coefficient (xlogp) -0.8
Rotatable Bond Count (rotbonds) 1
Hydrogen Bond Donor Count (hbonddonor) 2
Hydrogen Bond Acceptor Count (hbondacc) 2
Chemical Identifiers
Formula
C3H6N2
IUPAC Name
prop-2-ynylhydrazine
Canonical SMILES
C#CCNN
InChI
InChI=1S/C3H6N2/c1-2-3-5-4/h1,5H,3-4H2
InChIKey
CYCYZHMUOSKYBG-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

110 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) LDTP04232 12.00  LDD0385 
4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) LDTP04232 12.00  LDD0385 
5'-3' exonuclease PLD3 (PLD3) LDTP08476 12.00  LDD0385 
5'-3' exonuclease PLD3 (PLD3) LDTP08476 12.00  LDD0385 
5'-nucleotidase domain-containing protein 2 (NT5DC2) LDTP18414 11.00  LDD0385 
5'-nucleotidase domain-containing protein 2 (NT5DC2) LDTP18414 11.00  LDD0385 
Acid ceramidase (ASAH1) LDTP05905 20.00  LDD0387 
Acid ceramidase (ASAH1) LDTP05905 20.00  LDD0387 
Acyl-coenzyme A thioesterase 9, mitochondrial (ACOT9) LDTP16274 3.00  LDD0388 
Acyl-coenzyme A thioesterase 9, mitochondrial (ACOT9) LDTP16274 3.00  LDD0388 
Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) LDTP03560 6.00  LDD0385 
Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) LDTP03560 6.00  LDD0385 
Aldehyde dehydrogenase, mitochondrial (ALDH2) LDTP02074 8.00  LDD0385 
Aldehyde dehydrogenase, mitochondrial (ALDH2) LDTP02074 8.00  LDD0385 
Alpha-galactosidase A (GLA) LDTP02141 11.00  LDD0385 
Alpha-galactosidase A (GLA) LDTP02141 11.00  LDD0385 
Beta-galactosidase (GLB1) LDTP02837 12.00  LDD0385 
Beta-galactosidase (GLB1) LDTP02837 12.00  LDD0385 
Beta-glucuronidase (GUSB) LDTP02260 4.00  LDD0388 
Beta-glucuronidase (GUSB) LDTP02260 4.00  LDD0388 
Beta-hexosaminidase subunit beta (HEXB) LDTP02216 12.00  LDD0387 
Beta-hexosaminidase subunit beta (HEXB) LDTP02216 12.00  LDD0387 
Bleomycin hydrolase (BLMH) LDTP05992 9.00  LDD0385 
Bleomycin hydrolase (BLMH) LDTP05992 9.00  LDD0385 
Branched-chain-amino-acid aminotransferase, cytosolic (BCAT1) LDTP04622 4.00  LDD0388 
Branched-chain-amino-acid aminotransferase, cytosolic (BCAT1) LDTP04622 4.00  LDD0388 
Cathepsin B (CTSB) LDTP02223 9.00  LDD0387 
Cathepsin B (CTSB) LDTP02223 9.00  LDD0387 
Cathepsin D (CTSD) LDTP02198 13.00  LDD0385 
Cathepsin D (CTSD) LDTP02198 13.00  LDD0385 
Cathepsin S (CTSS) LDTP03329 4.00  LDD0388 
Cathepsin S (CTSS) LDTP03329 4.00  LDD0388 
Cathepsin Z (CTSZ) LDTP13147 13.00  LDD0387 
Cathepsin Z (CTSZ) LDTP13147 13.00  LDD0387 
Cytosol aminopeptidase (LAP3) LDTP03455 3.00  LDD0388 
Cytosol aminopeptidase (LAP3) LDTP03455 3.00  LDD0388 
DCN1-like protein 5 (DCUN1D5) LDTP11199 6.00  LDD0385 
DCN1-like protein 5 (DCUN1D5) LDTP11199 6.00  LDD0385 
Dipeptidyl peptidase 1 (CTSC) LDTP04572 3.00  LDD0386 
Dipeptidyl peptidase 1 (CTSC) LDTP04572 3.00  LDD0386 
FAST kinase domain-containing protein 5, mitochondrial (FASTKD5) LDTP07979 18.00  LDD0385 
FAST kinase domain-containing protein 5, mitochondrial (FASTKD5) LDTP07979 18.00  LDD0385 
Flavin reductase (NADPH) (BLVRB) LDTP03511 20.00  LDD0385 
Flavin reductase (NADPH) (BLVRB) LDTP03511 20.00  LDD0385 
Fructose-bisphosphate aldolase A (ALDOA) LDTP02008 16.00  LDD0385 
Fructose-bisphosphate aldolase A (ALDOA) LDTP02008 16.00  LDD0385 
Fructose-bisphosphate aldolase C (ALDOC) LDTP02386 16.00  LDD0385 
Fructose-bisphosphate aldolase C (ALDOC) LDTP02386 16.00  LDD0385 
Gamma-glutamyl hydrolase (GGH) LDTP09886 5.00  LDD0387 
Gamma-glutamyl hydrolase (GGH) LDTP09886 5.00  LDD0387 
Inorganic pyrophosphatase 2, mitochondrial (PPA2) LDTP11842 7.00  LDD0385 
Inorganic pyrophosphatase 2, mitochondrial (PPA2) LDTP11842 7.00  LDD0385 
Kynurenine--oxoglutarate transaminase 3 (KYAT3) LDTP07763 10.00  LDD0385 
Kynurenine--oxoglutarate transaminase 3 (KYAT3) LDTP07763 10.00  LDD0385 
Legumain (LGMN) LDTP10888 20.00  LDD0385 
Legumain (LGMN) LDTP10888 20.00  LDD0385 
Leucine carboxyl methyltransferase 1 (LCMT1) LDTP13337 3.00  LDD0386 
Leucine carboxyl methyltransferase 1 (LCMT1) LDTP13337 3.00  LDD0386 
Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) LDTP03858 7.00  LDD0387 
Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) LDTP03858 7.00  LDD0387 
Lysosomal alpha-glucosidase (GAA) LDTP02486 9.00  LDD0387 
Lysosomal alpha-glucosidase (GAA) LDTP02486 9.00  LDD0387 
Lysosomal alpha-mannosidase (MAN2B1) LDTP00338 20.00  LDD0387 
Lysosomal alpha-mannosidase (MAN2B1) LDTP00338 20.00  LDD0387 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 3.00  LDD0388 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 3.00  LDD0388 
Lysosomal protective protein (CTSA) LDTP02505 4.00  LDD0388 
Lysosomal protective protein (CTSA) LDTP02505 4.00  LDD0388 
Matrix metalloproteinase-14 (MMP14) LDTP04352 13.00  LDD0388 
Matrix metalloproteinase-14 (MMP14) LDTP04352 13.00  LDD0388 
Monofunctional C1-tetrahydrofolate synthase, mitochondrial (MTHFD1L) LDTP07651 3.00  LDD0388 
Monofunctional C1-tetrahydrofolate synthase, mitochondrial (MTHFD1L) LDTP07651 3.00  LDD0388 
Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 (PLOD3) LDTP01005 14.00  LDD0387 
Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 (PLOD3) LDTP01005 14.00  LDD0387 
N-acetylglucosamine-6-sulfatase (GNS) LDTP02799 10.00  LDD0387 
N-acetylglucosamine-6-sulfatase (GNS) LDTP02799 10.00  LDD0387 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 5.00  LDD0388 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 5.00  LDD0388 
Presequence protease, mitochondrial (PITRM1) LDTP07003 12.00  LDD0387 
Presequence protease, mitochondrial (PITRM1) LDTP07003 12.00  LDD0387 
Probable arginine--tRNA ligase, mitochondrial (RARS2) LDTP07092 16.00  LDD0385 
Probable arginine--tRNA ligase, mitochondrial (RARS2) LDTP07092 16.00  LDD0385 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 13.00  LDD0385 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 13.00  LDD0385 
Procathepsin L (CTSL) LDTP02217 20.00  LDD0387 
Procathepsin L (CTSL) LDTP02217 20.00  LDD0387 
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) LDTP05373 6.00  LDD0387 
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) LDTP05373 6.00  LDD0387 
Prolyl 3-hydroxylase 1 (P3H1) LDTP06750 8.00  LDD0387 
Prolyl 3-hydroxylase 1 (P3H1) LDTP06750 8.00  LDD0387 
Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) LDTP02679 15.00  LDD0387 
Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) LDTP02679 15.00  LDD0387 
Putative transferase CAF17, mitochondrial (IBA57) LDTP07110 14.00  LDD0385 
Putative transferase CAF17, mitochondrial (IBA57) LDTP07110 14.00  LDD0385 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 5.00  LDD0385 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 5.00  LDD0385 
Ribonuclease T2 (RNASET2) LDTP00316 3.00  LDD0386 
Ribonuclease T2 (RNASET2) LDTP00316 3.00  LDD0386 
Ribose-5-phosphate isomerase (RPIA) LDTP04236 3.00  LDD0386 
Ribose-5-phosphate isomerase (RPIA) LDTP04236 3.00  LDD0386 
S-adenosylmethionine decarboxylase proenzyme (AMD1) LDTP02918 20.00  LDD0385 
S-adenosylmethionine decarboxylase proenzyme (AMD1) LDTP02918 20.00  LDD0385 
Secernin-2 (SCRN2) LDTP15725 20.00  LDD0385 
Secernin-2 (SCRN2) LDTP15725 20.00  LDD0385 
Secernin-3 (SCRN3) LDTP17022 20.00  LDD0385 
Secernin-3 (SCRN3) LDTP17022 20.00  LDD0385 
Sepiapterin reductase (SPR) LDTP03730 5.00  LDD0387 
Sepiapterin reductase (SPR) LDTP03730 5.00  LDD0387 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 15.00  LDD0387 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 15.00  LDD0387 
------------------------------------------------------------------------------------
⏷ Show the Full List of 110 Enzyme
14 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Amyloid-beta precursor protein (APP) LDTP02071 16.00  LDD0387 
Amyloid-beta precursor protein (APP) LDTP02071 16.00  LDD0387 
Cation-independent mannose-6-phosphate receptor (IGF2R) LDTP02586 20.00  LDD0387 
Cation-independent mannose-6-phosphate receptor (IGF2R) LDTP02586 20.00  LDD0387 
Lysosome-associated membrane glycoprotein 2 (LAMP2) LDTP02655 12.00  LDD0387 
Lysosome-associated membrane glycoprotein 2 (LAMP2) LDTP02655 12.00  LDD0387 
Prosaposin (PSAP) LDTP02215 3.00  LDD0386 
Prosaposin (PSAP) LDTP02215 3.00  LDD0386 
Transferrin receptor protein 1 (TFRC) LDTP01969 3.00  LDD0388 
Transferrin receptor protein 1 (TFRC) LDTP01969 3.00  LDD0388 
Vesicle-associated membrane protein-associated protein A (VAPA) LDTP12966 20.00  LDD0387 
Vesicle-associated membrane protein-associated protein A (VAPA) LDTP12966 20.00  LDD0387 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 6.00  LDD0387 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 6.00  LDD0387 
------------------------------------------------------------------------------------
⏷ Show the Full List of 14 Transporter and channel
40 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Amyloid beta precursor like protein 2 (APLP2) LDTP05491 19.00  LDD0387 
Amyloid beta precursor like protein 2 (APLP2) LDTP05491 19.00  LDD0387 
Calponin-2 (CNN2) LDTP10864 4.00  LDD0388 
Calponin-2 (CNN2) LDTP10864 4.00  LDD0388 
Dedicator of cytokinesis protein 7 (DOCK7) LDTP10613 5.00  LDD0388 
Dedicator of cytokinesis protein 7 (DOCK7) LDTP10613 5.00  LDD0388 
Density-regulated protein (DENR) LDTP00808 5.00  LDD0387 
Density-regulated protein (DENR) LDTP00808 5.00  LDD0387 
Galectin-3-binding protein (LGALS3BP) LDTP05558 7.00  LDD0387 
Galectin-3-binding protein (LGALS3BP) LDTP05558 7.00  LDD0387 
Insulin receptor substrate 4 (IRS4) LDTP00398 20.00  LDD0385 
Insulin receptor substrate 4 (IRS4) LDTP00398 20.00  LDD0385 
Kelch-like ECH-associated protein 1 (KEAP1) LDTP06051 17.00  LDD0385 
Kelch-like ECH-associated protein 1 (KEAP1) LDTP06051 17.00  LDD0385 
Lamina-associated polypeptide 2, isoform alpha (TMPO) LDTP03967 9.00  LDD0387 
Lamina-associated polypeptide 2, isoform alpha (TMPO) LDTP03967 9.00  LDD0387 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 20.00  LDD0387 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 20.00  LDD0387 
Melanoma-associated antigen D2 (MAGED2) LDTP13657 6.00  LDD0388 
Melanoma-associated antigen D2 (MAGED2) LDTP13657 6.00  LDD0388 
Nonsense-mediated mRNA decay factor SMG9 (SMG9) LDTP11737 16.00  LDD0387 
Nonsense-mediated mRNA decay factor SMG9 (SMG9) LDTP11737 16.00  LDD0387 
NSFL1 cofactor p47 (NSFL1C) LDTP13684 8.00  LDD0387 
NSFL1 cofactor p47 (NSFL1C) LDTP13684 8.00  LDD0387 
Plasminogen activator inhibitor 1 (SERPINE1) LDTP02085 17.00  LDD0387 
Plasminogen activator inhibitor 1 (SERPINE1) LDTP02085 17.00  LDD0387 
Progranulin (GRN) LDTP03453 20.00  LDD0387 
Progranulin (GRN) LDTP03453 20.00  LDD0387 
Protein canopy homolog 3 (CNPY3) LDTP11176 5.00  LDD0387 
Protein canopy homolog 3 (CNPY3) LDTP11176 5.00  LDD0387 
Protein CREG1 (CREG1) LDTP01279 20.00  LDD0387 
Protein CREG1 (CREG1) LDTP01279 20.00  LDD0387 
Protein FAM50A (FAM50A) LDTP06105 5.00  LDD0387 
Protein FAM50A (FAM50A) LDTP06105 5.00  LDD0387 
Tetratricopeptide repeat protein 38 (TTC38) LDTP19372 4.00  LDD0386 
Tetratricopeptide repeat protein 38 (TTC38) LDTP19372 4.00  LDD0386 
Thrombospondin-1 (THBS1) LDTP02238 16.00  LDD0387 
Thrombospondin-1 (THBS1) LDTP02238 16.00  LDD0387 
Zinc finger protein 185 (ZNF185) LDTP00580 20.00  LDD0388 
Zinc finger protein 185 (ZNF185) LDTP00580 20.00  LDD0388 
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⏷ Show the Full List of 40 Other

Competitor(s) Related To This Probe

Competitor ID Name Concentration Cell-system Ref
 LDCM0099  Phenelzine 10 mM Human normal cells (HEK-293T) [1]

Full Information of The Labelling Profiles of This Probe

Quantification: Probe vs DMSO
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 mM
Quantitative Method
SILAC
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Interaction Atlas ID
 LDD0385 
Download The Altas
  
Experiment 2 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 mM
Quantitative Method
SILAC
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Breast
Disease Model
Breast adenocarcinoma [ICD-11:2C60]
Model Name
Human breast adenocarcinoma cells (MDA-MB-231)
Interaction Atlas ID
 LDD0387 
Download The Altas
Quantification: Probe vs (Probe+Competitor)
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 mM
Quantitative Method
SILAC
Competitor Name
Phenelzine
 Competitor Info 
Competitor Concentration
10 mM
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Interaction Atlas ID
 LDD0386 
Download The Altas
  
Experiment 2 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 mM
Quantitative Method
SILAC
Competitor Name
Phenelzine
 Competitor Info 
Competitor Concentration
10 mM
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Breast
Disease Model
Breast adenocarcinoma [ICD-11:2C60]
Model Name
Human breast adenocarcinoma cells (MDA-MB-231)
Interaction Atlas ID
 LDD0388 
Download The Altas

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.