Details of the Target

General Information of Target

Target ID LDTP00338
Target Name Lysosomal alpha-mannosidase (MAN2B1)
Gene Name MAN2B1
Gene ID 4125
Synonyms
LAMAN; MANB; Lysosomal alpha-mannosidase; Laman; EC 3.2.1.24; Lysosomal acid alpha-mannosidase; Mannosidase alpha class 2B member 1; Mannosidase alpha-B) [Cleaved into: Lysosomal alpha-mannosidase A peptide; Lysosomal alpha-mannosidase B peptide; Lysosomal alpha-mannosidase C peptide; Lysosomal alpha-mannosidase D peptide; Lysosomal alpha-mannosidase E peptide]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQP
NMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVE
IAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLE
DTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWR
ASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVA
TAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVHVLYSTPACYL
WELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG
LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNAL
ARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDP
NGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRS
WSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAY
IFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIP
VGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNT
RIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG
RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSV
HLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQ
LREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG
Target Type
Successful
Target Bioclass
Enzyme
Family
Glycosyl hydrolase 38 family
Subcellular location
Lysosome
Function Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. Cleaves all known types of alpha-mannosidic linkages.
TTD ID
T63156
Uniprot ID
O00754
DrugMap ID
TTC12RO
Ensemble ID
ENST00000221363.8
HGNC ID
HGNC:6826
ChEMBL ID
CHEMBL4059

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HS936T SNV: p.P553S .
IM95 Deletion: p.G801AfsTer4 .
JURKAT SNV: p.S547N .
LS123 SNV: p.T452P .
MOLT4 SNV: p.R527W .
NCIH358 SNV: p.R732L .
OCUG1 SNV: p.A958G .
OVCAR5 SNV: p.R841L .
RKO SNV: p.V1004A .
RL SNV: p.T452P .
RL952 SNV: p.R308H .
SNU1 Deletion: p.G801AfsTer4 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 1
 Probe Info  		20.00  LDD0387  [1]
Alkylaryl probe 2
 Probe Info  		20.00  LDD0391  [1]
SAA-alkyne
 Probe Info  		1.08  LDD0252  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
FBPP2
 Probe Info  		7.62  LDD0054  [4]
PAL-AfBPP Probe
Click To Hide/Show 24 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		5.62  LDD1711  [5]
C045
 Probe Info  		7.46  LDD1744  [5]
C106
 Probe Info  		16.56  LDD1793  [5]
C108
 Probe Info  		8.22  LDD1795  [5]
C115
 Probe Info  		5.90  LDD1802  [5]
C130
 Probe Info  		6.50  LDD1812  [5]
C141
 Probe Info  		10.13  LDD1823  [5]
C149
 Probe Info  		6.87  LDD1830  [5]
C170
 Probe Info  		18.64  LDD1850  [5]
C171
 Probe Info  		7.41  LDD1851  [5]
C186
 Probe Info  		10.20  LDD1864  [5]
C208
 Probe Info  		6.02  LDD1883  [5]
C223
 Probe Info  		5.54  LDD1897  [5]
C237
 Probe Info  		6.63  LDD1910  [5]
C302
 Probe Info  		5.28  LDD1971  [5]
C310
 Probe Info  		14.12  LDD1977  [5]
C343
 Probe Info  		11.16  LDD2005  [5]
C346
 Probe Info  		11.16  LDD2007  [5]
C376
 Probe Info  		8.57  LDD2036  [5]
FFF probe12
 Probe Info  		20.00  LDD0473  [6]
FFF probe14
 Probe Info  		20.00  LDD0477  [6]
FFF probe9
 Probe Info  		20.00  LDD0470  [6]
JN0003
 Probe Info  		20.00  LDD0469  [6]
VE-P
 Probe Info  		N.A.  LDD0396  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0099  Phenelzine MDA-MB-231 8.00  LDD0388  [1]
 LDCM0008  Tranylcypromine SH-SY5Y 7.62  LDD0054  [4]

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
7 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.