Details of the Target

General Information of Target

Target ID LDTP13684
Target Name NSFL1 cofactor p47 (NSFL1C)
Gene Name NSFL1C
Gene ID 55968
Synonyms
UBXN2C; NSFL1 cofactor p47; UBX domain-containing protein 2C; p97 cofactor p47
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MEPPGPVRGPLQDSSWYEPSAELVQTRMAVSLTAAETLALQGTQGQEKMMMMGPKEEEQS
CEYETRLPGNHSTSQEIFRQRFRHLRYQETPGPREALSQLRVLCCEWLRPEKHTKEQILE
FLVLEQFLTILPEELQSWVRGHHPKSGEEAVTVLEDLEKGLEPEPQVPGPAHGPAQEEPW
EKKESLGAAQEALSIQLQPKETQPFPKSEQVYLHFLSVVTEDGPEPKDKGSLPQPPITEV
ESQVFSEKLATDTSTFEATSEGTLELQQRNPKAERLRWSPAQEESFRQMVVIHKEIPTGK
KDHECSECGKTFIYNSHLVVHQRVHSGEKPYKCSDCGKTFKQSSNLGQHQRIHTGEKPFE
CNECGKAFRWGAHLVQHQRIHSGEKPYECNECGKAFSQSSYLSQHRRIHSGEKPFICKEC
GKAYGWCSELIRHRRVHARKEPSH
Target Bioclass
Other
Family
NSFL1C family
Subcellular location
Nucleus
Function
Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.
Uniprot ID
Q9UNZ2
Ensemble ID
ENST00000216879.9
HGNC ID
HGNC:15912

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
EFO27 SNV: p.P330S .
HCT15 SNV: p.T295I .
LNCaP clone FGC SNV: p.S315I .
LS123 Insertion: p.S315QfsTer36 .
LS180 Deletion: p.Q266RfsTer3 .
NCIH1993 SNV: p.R329W .
SW1271 SNV: p.G305R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 1
 Probe Info  		8.00  LDD0387  [1]
Probe 1
 Probe Info  		Y167(77.61)  LDD3495  [2]
HHS-475
 Probe Info  		Y167(0.61); Y95(0.99)  LDD0264  [3]
HHS-465
 Probe Info  		Y155(9.34); Y167(6.08); Y95(4.45)  LDD2237  [4]
AMP probe
 Probe Info  		N.A.  LDD0200  [5]
ATP probe
 Probe Info  		K172(0.00); K124(0.00); K127(0.00); K112(0.00)  LDD0199  [5]
m-APA
 Probe Info  		N.A.  LDD2232  [6]
NHS
 Probe Info  		K172(0.00); K251(0.00)  LDD0010  [7]
SF
 Probe Info  		N.A.  LDD0028  [8]
STPyne
 Probe Info  		N.A.  LDD0009  [7]
1c-yne
 Probe Info  		N.A.  LDD0228  [9]
Acrolein
 Probe Info  		N.A.  LDD0217  [10]
HHS-482
 Probe Info  		Y155(1.51); Y167(0.96); Y95(1.01)  LDD2239  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C087
 Probe Info  		7.26  LDD1779  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [10]
 LDCM0116  HHS-0101 DM93 Y167(0.61); Y95(0.99)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y95(0.87); Y167(0.91)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y167(0.65); Y95(0.93)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y167(0.49); Y95(0.91)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y167(0.69); Y95(0.82)  LDD0268  [3]
 LDCM0107  IAA HeLa N.A.  LDD0221  [10]
 LDCM0109  NEM HeLa N.A.  LDD0224  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transitional endoplasmic reticulum ATPase (VCP) AAA ATPase family P55072
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TAR DNA-binding protein 43 (TARDBP) . Q13148

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587