Details of the Target

General Information of Target

Target ID LDTP16274
Target Name Acyl-coenzyme A thioesterase 9, mitochondrial (ACOT9)
Gene Name ACOT9
Gene ID 23597
Synonyms
Acyl-coenzyme A thioesterase 9, mitochondrial; Acyl-CoA thioesterase 9; EC 3.1.2.-; Acyl-CoA thioester hydrolase 9
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MGGLRPWSRYGLLVVAHLLALGLGAVVFQALEGPPACRLQAELRAELAAFQAEHRACLPP
GALEELLGTALATQAHGVSTLGNSSEGRTWDLPSALLFAASILTTTGYGHMAPLSPGGKA
FCMVYAALGLPASLALVATLRHCLLPVLSRPRAWVAVHWQLSPARAALLQAVALGLLVAS
SFVLLPALVLWGLQGDCSLLGAVYFCFSSLSTIGLEDLLPGRGRSLHPVIYHLGQLALLG
YLLLGLLAMLLAVETFSELPQVRAMGKFFRPSGPVTAEDQGGILGQDELALSTLPPAAPA
SGQAPAC
Target Bioclass
Enzyme
Family
Acyl coenzyme A hydrolase family
Subcellular location
Mitochondrion
Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.
Uniprot ID
Q9Y305
Ensemble ID
ENST00000336430.11
HGNC ID
HGNC:17152
ChEMBL ID
CHEMBL4295987

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 29 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		14.83  LDD0402  [1]
CY-1
 Probe Info  		100.00  LDD0243  [2]
CY4
 Probe Info  		100.00  LDD0244  [2]
N1
 Probe Info  		100.00  LDD0242  [2]
W1
 Probe Info  		19.18  LDD0235  [3]
TH211
 Probe Info  		Y104(13.74)  LDD0257  [4]
STPyne
 Probe Info  		K103(6.55); K157(8.43); K237(6.29); K250(5.73)  LDD0277  [5]
ONAyne
 Probe Info  		K294(7.14)  LDD0275  [5]
Alkylaryl probe 1
 Probe Info  		3.00  LDD0388  [6]
Alkylaryl probe 2
 Probe Info  		3.00  LDD0392  [6]
DBIA
 Probe Info  		C308(1.66)  LDD3310  [7]
BTD
 Probe Info  		C299(2.44)  LDD1700  [8]
AHL-Pu-1
 Probe Info  		C299(2.26)  LDD0170  [9]
HPAP
 Probe Info  		3.03  LDD0062  [10]
AMP probe
 Probe Info  		N.A.  LDD0200  [11]
ATP probe
 Probe Info  		N.A.  LDD0199  [11]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [12]
NAIA_4
 Probe Info  		C155(0.00); C194(0.00); C299(0.00)  LDD2226  [13]
TFBX
 Probe Info  		N.A.  LDD0027  [12]
1d-yne
 Probe Info  		N.A.  LDD0356  [14]
IA-alkyne
 Probe Info  		N.A.  LDD0151  [15]
IPM
 Probe Info  		N.A.  LDD0005  [16]
Phosphinate-6
 Probe Info  		C299(0.00); C155(0.00)  LDD0018  [17]
Acrolein
 Probe Info  		C299(0.00); H374(0.00)  LDD0217  [18]
Cinnamaldehyde
 Probe Info  		N.A.  LDD0220  [18]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [18]
Methacrolein
 Probe Info  		N.A.  LDD0218  [18]
AOyne
 Probe Info  		15.00  LDD0443  [19]
NAIA_5
 Probe Info  		C128(0.00); C194(0.00); C155(0.00); C299(0.00)  LDD2223  [13]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		20.00  LDD0475  [20]
FFF probe3
 Probe Info  		10.23  LDD0465  [20]
STS-2
 Probe Info  		N.A.  LDD0139  [21]
VE-P
 Probe Info  		N.A.  LDD0396  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C299(0.68)  LDD2142  [8]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C299(0.98)  LDD2112  [8]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C299(0.87)  LDD2130  [8]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C299(1.37)  LDD2152  [8]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C299(0.77)  LDD2103  [8]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C299(0.58)  LDD2132  [8]
 LDCM0025  4SU-RNA DM93 C299(2.26)  LDD0170  [9]
 LDCM0026  4SU-RNA+native RNA DM93 C155(2.93)  LDD0171  [9]
 LDCM0545  Acetamide MDA-MB-231 C299(0.80)  LDD2138  [8]
 LDCM0156  Aniline NCI-H1299 11.64  LDD0403  [1]
 LDCM0630  CCW28-3 231MFP C155(1.15)  LDD2214  [23]
 LDCM0108  Chloroacetamide HeLa C299(0.00); H374(0.00)  LDD0222  [18]
 LDCM0632  CL-Sc Hep-G2 C155(0.45)  LDD2227  [13]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C299(5.25)  LDD1702  [8]
 LDCM0625  F8 Ramos C299(0.55)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C299(1.54)  LDD2189  [24]
 LDCM0573  Fragment11 Ramos C299(0.41)  LDD2190  [24]
 LDCM0574  Fragment12 Ramos C299(1.90)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C299(1.14)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C299(0.52)  LDD2193  [24]
 LDCM0580  Fragment21 Ramos C299(0.97)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C299(1.00)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C299(0.92)  LDD2197  [24]
 LDCM0586  Fragment28 Ramos C299(0.77)  LDD2198  [24]
 LDCM0588  Fragment30 Ramos C299(1.26)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C299(0.92)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C299(1.14)  LDD2201  [24]
 LDCM0468  Fragment33 Ramos C299(0.89)  LDD2202  [24]
 LDCM0596  Fragment38 Ramos C299(1.29)  LDD2203  [24]
 LDCM0614  Fragment56 Ramos C299(0.90)  LDD2205  [24]
 LDCM0015  HNE MDA-MB-231 C155(1.09); C128(1.28)  LDD0346  [24]
 LDCM0107  IAA HeLa C299(0.00); H262(0.00); H374(0.00)  LDD0221  [18]
 LDCM0022  KB02 22RV1 C308(1.27)  LDD2243  [7]
 LDCM0023  KB03 MDA-MB-231 C299(14.50); C328(1.45)  LDD1701  [8]
 LDCM0024  KB05 COLO792 C308(1.66)  LDD3310  [7]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C299(0.85)  LDD2121  [8]
 LDCM0109  NEM HeLa H262(0.00); H421(0.00); H374(0.00)  LDD0223  [18]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C299(1.00)  LDD2089  [8]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C299(1.08)  LDD2092  [8]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C299(1.10)  LDD2093  [8]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C299(1.05)  LDD2097  [8]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C299(0.82)  LDD2098  [8]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C299(1.14)  LDD2099  [8]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C299(0.64)  LDD2100  [8]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C299(1.07)  LDD2101  [8]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C299(0.58)  LDD2104  [8]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C299(1.43)  LDD2105  [8]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C299(0.68)  LDD2106  [8]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C299(0.97)  LDD2107  [8]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C299(0.81)  LDD2109  [8]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C299(1.16)  LDD2111  [8]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C299(0.76)  LDD2114  [8]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C299(0.48)  LDD2115  [8]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C299(1.14)  LDD2123  [8]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C299(0.78)  LDD2125  [8]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C299(1.01)  LDD2127  [8]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C299(0.64)  LDD2128  [8]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C299(0.97)  LDD2129  [8]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C299(0.59)  LDD2133  [8]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C299(0.55)  LDD2134  [8]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C299(1.27)  LDD2135  [8]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C299(0.74)  LDD2136  [8]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C299(0.98)  LDD2137  [8]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C299(2.44)  LDD1700  [8]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C299(1.01)  LDD2140  [8]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C299(0.91)  LDD2141  [8]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C299(1.77)  LDD2144  [8]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C299(2.42)  LDD2145  [8]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C299(1.07)  LDD2146  [8]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C299(0.83)  LDD2150  [8]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C299(1.60)  LDD2153  [8]
 LDCM0627  NUDT7-COV-1 HEK-293T C299(1.93)  LDD2206  [25]
 LDCM0628  OTUB2-COV-1 HEK-293T C299(0.63)  LDD2207  [25]
 LDCM0014  Panhematin HEK-293T 3.03  LDD0062  [10]
 LDCM0099  Phenelzine MDA-MB-231 3.00  LDD0388  [6]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
8 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
9 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
10 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
11 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
12 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
13 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
14 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
15 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
16 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
17 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
18 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
19 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
20 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
23 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.