Details of the Probe

General Information of Probe

Probe ID
LDPC0640
Probe Name
C425
Synonyms
Z4225955998
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 292.38
Lipid-water partition coefficient (xlogp) 1.2
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 5
Chemical Identifiers
Formula
C15H24N4O2
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-2-(1-methylpiperidin-4-yl)oxyacetamide
Canonical SMILES
CN1CCC(CC1)OCC(=O)NCCC2(N=N2)CCC#C
InChI
InChI=1S/C15H24N4O2/c1-3-4-7-15(17-18-15)8-9-16-14(20)12-21-13-5-10-19(2)11-6-13/h1,13H,4-12H2,2H3,(H,16,20)
InChIKey
RLYYWELQDBZTQV-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

38 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
5'-3' exonuclease PLD3 (PLD3) LDTP08476 7.11  LDD2080 
7-dehydrocholesterol reductase (DHCR7) LDTP13131 7.06  LDD2080 
Acid ceramidase (ASAH1) LDTP05905 8.46  LDD2080 
Alpha-galactosidase A (GLA) LDTP02141 22.63  LDD2080 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 20.68  LDD2080 
Beta-galactosidase (GLB1) LDTP02837 13.55  LDD2080 
Beta-glucuronidase (GUSB) LDTP02260 6.92  LDD2080 
Beta-hexosaminidase subunit alpha (HEXA) LDTP02169 17.88  LDD2080 
Beta-hexosaminidase subunit beta (HEXB) LDTP02216 10.48  LDD2080 
Beta-mannosidase (MANBA) LDTP00282 10.78  LDD2080 
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 26.35  LDD2080 
Carboxypeptidase D (CPD) LDTP01372 10.20  LDD2080 
Cathepsin B (CTSB) LDTP02223 47.18  LDD2080 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 11.79  LDD2080 
Delta(24)-sterol reductase (DHCR24) LDTP06375 23.26  LDD2080 
Dipeptidyl peptidase 1 (CTSC) LDTP04572 10.48  LDD2080 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 16.91  LDD2080 
Gamma-glutamyl hydrolase (GGH) LDTP09886 13.45  LDD2080 
Leucyl-cystinyl aminopeptidase (LNPEP) LDTP13358 18.51  LDD2080 
Lysosomal acid glucosylceramidase (GBA1) LDTP02005 5.35  LDD2080 
Lysosomal acid phosphatase (ACP2) LDTP02539 17.88  LDD2080 
Lysosomal alpha-glucosidase (GAA) LDTP02486 17.39  LDD2080 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 11.39  LDD2080 
Lysosomal protective protein (CTSA) LDTP02505 11.08  LDD2080 
Lysosomal thioesterase PPT2 (PPT2) LDTP13620 20.39  LDD2080 
N-acetylglucosamine-6-sulfatase (GNS) LDTP02799 22.32  LDD2080 
N-alpha-acetyltransferase 40 (NAA40) LDTP08265 5.78  LDD2080 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 15.14  LDD2080 
Peripheral plasma membrane protein CASK (CASK) LDTP00482 7.57  LDD2080 
Phospholipase A2 group XV (PLA2G15) LDTP09088 9.51  LDD2080 
Plasma alpha-L-fucosidase (FUCA2) LDTP11215 19.16  LDD2080 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 8.63  LDD2080 
Prostaglandin reductase 3 (PTGR3) LDTP15422 11.08  LDD2080 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 22.47  LDD2080 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 20.39  LDD2080 
Ribonuclease T2 (RNASET2) LDTP00316 25.11  LDD2080 
Tissue alpha-L-fucosidase (FUCA1) LDTP02006 10.70  LDD2080 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 31.34  LDD2080 
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⏷ Show the Full List of 38 Enzyme
22 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Cation-independent mannose-6-phosphate receptor (IGF2R) LDTP02586 14.52  LDD2080 
H(+)/Cl(-) exchange transporter 3 (CLCN3) LDTP04459 7.11  LDD2080 
Integral membrane protein 2B (ITM2B) LDTP13833 13.36  LDD2080 
Lysosome membrane protein 2 (SCARB2) LDTP06034 16.34  LDD2080 
Lysosome-associated membrane glycoprotein 1 (LAMP1) LDTP02562 10.41  LDD2080 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 5.28  LDD2080 
Mpv17-like protein 2 (MPV17L2) LDTP14952 5.28  LDD2080 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 8.46  LDD2080 
NPC intracellular cholesterol transporter 2 (NPC2) LDTP04940 19.16  LDD2080 
Podocalyxin (PODXL) LDTP00321 10.56  LDD2080 
Prolow-density lipoprotein receptor-related protein 1 (LRP1) LDTP05538 10.93  LDD2080 
Prosaposin (PSAP) LDTP02215 20.11  LDD2080 
Protein tweety homolog 3 (TTYH3) LDTP11615 15.89  LDD2080 
Proton-coupled amino acid transporter 1 (SLC36A1) LDTP08016 5.35  LDD2080 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 7.11  LDD2080 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 8.88  LDD2080 
Slit homolog 2 protein (SLIT2) LDTP01427 7.31  LDD2080 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 8.94  LDD2080 
Sortilin (SORT1) LDTP10885 13.00  LDD2080 
Transferrin receptor protein 1 (TFRC) LDTP01969 13.64  LDD2080 
V-type proton ATPase subunit S1 (ATP6AP1) LDTP06499 11.47  LDD2080 
Voltage-dependent calcium channel subunit alpha-2/delta-1 (CACNA2D1) LDTP04612 9.00  LDD2080 
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⏷ Show the Full List of 22 Transporter and channel
5 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
HLA class I histocompatibility antigen, A alpha chain (HLA-A) LDTP02040 9.99  LDD2080 
HLA class I histocompatibility antigen, B alpha chain (HLA-B) LDTP01913 9.51  LDD2080 
HLA class I histocompatibility antigen, C alpha chain (HLA-C) LDTP02491 5.62  LDD2080 
IgG receptor FcRn large subunit p51 (FCGRT) LDTP04720 18.51  LDD2080 
Junctional adhesion molecule A (F11R) LDTP14203 5.10  LDD2080 
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1 Cytokine and receptor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Interferon gamma receptor 1 (IFNGR1) LDTP02775 21.86  LDD2080 
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12 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Amyloid beta precursor like protein 2 (APLP2) LDTP05491 32.00  LDD2080 
Collectin-12 (COLEC12) LDTP07031 10.27  LDD2080 
Dyslexia-associated protein KIAA0319-like protein (KIAA0319L) LDTP08689 7.31  LDD2080 
Galectin-3-binding protein (LGALS3BP) LDTP05558 22.63  LDD2080 
Ganglioside GM2 activator (GM2A) LDTP02927 15.78  LDD2080 
Golgi apparatus protein 1 (GLG1) LDTP09920 6.06  LDD2080 
Golgi membrane protein 1 (GOLM1) LDTP09054 5.98  LDD2080 
Heat shock 70 kDa protein 13 (HSPA13) LDTP04207 6.19  LDD2080 
Nicastrin (NCSTN) LDTP09787 11.24  LDD2080 
Protein CutA (CUTA) LDTP01075 13.36  LDD2080 
Synaptic vesicle glycoprotein 2A (SV2A) LDTP07934 5.62  LDD2080 
Trans-Golgi network integral membrane protein 2 (TGOLN2) LDTP00779 11.16  LDD2080 
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⏷ Show the Full List of 12 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD2080 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587