Details of the Target

General Information of Target

Target ID LDTP14203
Target Name Junctional adhesion molecule A (F11R)
Gene Name F11R
Gene ID 50848
Synonyms
JAM1; JCAM; Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD antigen CD321
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKV
TAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSG
LFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGE
SGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVR
NDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPEL
IEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVM
HYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTV
QQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLC
INFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEE
CMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKN
KDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLN
KLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYA
DFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEM
RDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKI
KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEAD
ALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDD
LELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDK
VNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQL
NEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQR
SDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKH
ADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQL
SEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELK
RQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYE
TDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSN
AACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLE
TLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEG
KILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKK
KMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVER
RANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQ
IQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLR
LDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEE
DRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQ
VNKLRVKSREVHTKVISEE
Target Type
Literature-reported
Target Bioclass
Immunoglobulin
Family
Immunoglobulin superfamily
Subcellular location
Cell junction, tight junction
Function
Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.; (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.
TTD ID
T15438
Uniprot ID
Q9Y624
DrugMap ID
TT3C8EG
Ensemble ID
ENST00000368026.11
HGNC ID
HGNC:14685

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL33 SNV: p.M1? .
KMS11 SNV: p.M110I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K277(7.08)  LDD0277  [2]
OPA-S-S-alkyne
 Probe Info  		K63(2.75); K78(3.33); K47(5.07); K178(5.39)  LDD3494  [3]
Probe 1
 Probe Info  		Y75(42.19); Y280(18.89)  LDD3495  [4]
DBIA
 Probe Info  		C50(1.96)  LDD3310  [5]
BTD
 Probe Info  		C74(1.90)  LDD1700  [6]
IA-alkyne
 Probe Info  		C50(0.00); C153(0.00)  LDD0162  [7]
NAIA_5
 Probe Info  		C153(0.00); C50(0.00); C74(0.00)  LDD2223  [8]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C170
 Probe Info  		11.88  LDD1850  [9]
C186
 Probe Info  		7.62  LDD1864  [9]
C208
 Probe Info  		4.92  LDD1883  [9]
C302
 Probe Info  		6.82  LDD1971  [9]
C310
 Probe Info  		8.46  LDD1977  [9]
C425
 Probe Info  		5.10  LDD2080  [9]
FFF probe11
 Probe Info  		17.41  LDD0472  [10]
FFF probe12
 Probe Info  		20.00  LDD0473  [10]
VE-P
 Probe Info  		N.A.  LDD0396  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0371  CL102 HEK-293T C74(0.94)  LDD1575  [12]
 LDCM0375  CL106 HEK-293T C74(0.91)  LDD1579  [12]
 LDCM0380  CL110 HEK-293T C74(0.92)  LDD1584  [12]
 LDCM0384  CL114 HEK-293T C74(0.93)  LDD1588  [12]
 LDCM0388  CL118 HEK-293T C74(1.02)  LDD1592  [12]
 LDCM0393  CL122 HEK-293T C74(1.12)  LDD1597  [12]
 LDCM0397  CL126 HEK-293T C74(0.93)  LDD1601  [12]
 LDCM0401  CL14 HEK-293T C74(1.05)  LDD1605  [12]
 LDCM0407  CL2 HEK-293T C74(1.07)  LDD1611  [12]
 LDCM0414  CL26 HEK-293T C74(0.99)  LDD1618  [12]
 LDCM0441  CL50 HEK-293T C74(1.10)  LDD1645  [12]
 LDCM0454  CL62 HEK-293T C74(1.03)  LDD1657  [12]
 LDCM0467  CL74 HEK-293T C74(1.15)  LDD1670  [12]
 LDCM0480  CL86 HEK-293T C74(0.93)  LDD1683  [12]
 LDCM0493  CL98 HEK-293T C74(0.98)  LDD1696  [12]
 LDCM0427  Fragment51 HEK-293T C74(1.00)  LDD1631  [12]
 LDCM0022  KB02 A-549 C153(1.50)  LDD2260  [5]
 LDCM0023  KB03 A2780 C50(1.65)  LDD2671  [5]
 LDCM0024  KB05 COLO792 C50(1.96)  LDD3310  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C74(1.09)  LDD2099  [6]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C74(0.89)  LDD2107  [6]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C74(0.66)  LDD2109  [6]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C74(0.61)  LDD2115  [6]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C74(0.88)  LDD2123  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C74(1.10)  LDD2125  [6]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C74(0.68)  LDD2127  [6]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C74(0.60)  LDD2134  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C74(0.83)  LDD2136  [6]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C74(0.78)  LDD2137  [6]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C74(1.90)  LDD1700  [6]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C74(0.55)  LDD2148  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Methylthioribose-1-phosphate isomerase (MRI1) EIF-2B alpha/beta/delta subunits family Q9BV20
Matrix metalloproteinase-14 (MMP14) Peptidase M10A family P50281
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
E3 ubiquitin-protein ligase MARCHF2 (MARCHF2) . Q9P0N8
Transporter and channel
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proton-coupled zinc antiporter SLC30A2 (SLC30A2) Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family Q9BRI3
Myeloid-associated differentiation marker (MYADM) MAL family Q96S97
CMP-sialic acid transporter (SLC35A1) Nucleotide-sugar transporter family P78382
Prenylated Rab acceptor protein 1 (RABAC1) PRA1 family Q9UI14
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8
Guided entry of tail-anchored proteins factor CAMLG (CAMLG) . P49069
Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF (STRIT1) . P0DN84
Transmembrane protein 100 (TMEM100) . Q9NV29
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ameloblastin (AMBN) Ameloblastin family Q9NP70
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Partitioning defective 3 homolog (PARD3) PAR3 family Q8TEW0
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) SGT family O43765
Synaptosomal-associated protein 47 (SNAP47) SVAP1 family Q5SQN1
La-related protein 4B (LARP4B) . Q92615

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402