Probe Name	C334					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					8.5
LDTP00316	Ribonuclease T2 (RNASET2)					8.3
LDTP09256	Putative phospholipase B-like 2 (PLBD2)					7.8
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					7.2
LDTP01241	Bis(monoacylglycero)phosphate synthase CLN5 (CLN5)					7.1
LDTP02141	Alpha-galactosidase A (GLA)					7.0
LDTP02539	Lysosomal acid phosphatase (ACP2)					6.7
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					6.1
LDTP11215	Plasma alpha-L-fucosidase (FUCA2)					5.5
LDTP02486	Lysosomal alpha-glucosidase (GAA)					5.0
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					5.0

Target ID	Target Name					Binding Ratio
LDTP02071	Amyloid-beta precursor protein (APP)					10.1
LDTP11615	Protein tweety homolog 3 (TTYH3)					6.2
LDTP04940	NPC intracellular cholesterol transporter 2 (NPC2)					5.8
LDTP01969	Transferrin receptor protein 1 (TFRC)					5.6

Target ID	Target Name					Binding Ratio
LDTP07703	Seizure 6-like protein 2 (SEZ6L2)					15.7
LDTP05558	Galectin-3-binding protein (LGALS3BP)					8.3
LDTP14486	Clustered mitochondria protein homolog (CLUH)					5.6
LDTP02927	Ganglioside GM2 activator (GM2A)					5.0

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587