Probe Name	C184					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP13147	Cathepsin Z (CTSZ)					18.5
LDTP06375	Delta(24)-sterol reductase (DHCR24)					11.7
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					8.8
LDTP09256	Putative phospholipase B-like 2 (PLBD2)					8.4
LDTP10888	Legumain (LGMN)					8.2
LDTP09395	Signal peptide peptidase-like 2A (SPPL2A)					8.1
LDTP00316	Ribonuclease T2 (RNASET2)					7.7
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					7.5
LDTP09886	Gamma-glutamyl hydrolase (GGH)					6.5
LDTP00428	Tripeptidyl-peptidase 1 (TPP1)					6.4
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					6.3
LDTP02006	Tissue alpha-L-fucosidase (FUCA1)					5.8
LDTP02799	N-acetylglucosamine-6-sulfatase (GNS)					5.6
LDTP02539	Lysosomal acid phosphatase (ACP2)					5.3
LDTP12020	Histone-lysine N-methyltransferase SMYD3 (SMYD3)					5.2

Target ID	Target Name					Binding Ratio
LDTP16813	Tetraspanin-3 (TSPAN3)					7.2
LDTP11615	Protein tweety homolog 3 (TTYH3)					6.5
LDTP04940	NPC intracellular cholesterol transporter 2 (NPC2)					6.3
LDTP02071	Amyloid-beta precursor protein (APP)					6.1

Target ID	Target Name					Binding Ratio
LDTP09907	Neogenin (NEO1)					6.8

Target ID	Target Name					Binding Ratio
LDTP13606	Mammalian ependymin-related protein 1 (EPDR1)					6.4
LDTP05558	Galectin-3-binding protein (LGALS3BP)					6.0

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587