Details of the Target

General Information of Target

Target ID LDTP01640
Target Name CCR4-NOT transcription complex subunit 4 (CNOT4)
Gene Name CNOT4
Gene ID 4850
Synonyms
NOT4; CCR4-NOT transcription complex subunit 4; EC 2.3.2.27; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp; RING-type E3 ubiquitin transferase CNOT4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPY
PEDPAVYKPLSQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRL
ADPEVLKRPEYFGKFGKIHKVVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVV
VDGRTLKASLGTTKYCSYFLKNMQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKL
LQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPSDSLSIGNGDNSQQISNSDTP
SPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPPFPSSPQT
SSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIE
KELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQ
QHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIP
VAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA
Target Bioclass
Enzyme
Subcellular location
Cytoplasm
Function
Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy.
Uniprot ID
O95628
Ensemble ID
ENST00000315544.6
HGNC ID
HGNC:7880
ChEMBL ID
CHEMBL4105770

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K134(1.54)  LDD0277  [1]
DBIA
 Probe Info  		C175(3.11)  LDD3311  [2]
HHS-475
 Probe Info  		Y67(0.78)  LDD0264  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		C196(0.00); C175(0.00)  LDD0037  [4]
IPM
 Probe Info  		N.A.  LDD0025  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
NAIA_5
 Probe Info  		N.A.  LDD2225  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [5]
ENE
 Probe Info  		N.A.  LDD0006  [7]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [8]
PAL-AfBPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		7.84  LDD1728  [9]
C026
 Probe Info  		5.35  LDD1732  [9]
C095
 Probe Info  		5.43  LDD1786  [9]
C143
 Probe Info  		30.70  LDD1825  [9]
C191
 Probe Info  		26.72  LDD1868  [9]
C193
 Probe Info  		12.30  LDD1869  [9]
C197
 Probe Info  		5.10  LDD1873  [9]
C213
 Probe Info  		45.89  LDD1887  [9]
C214
 Probe Info  		5.86  LDD1888  [9]
C228
 Probe Info  		25.28  LDD1901  [9]
C238
 Probe Info  		16.68  LDD1911  [9]
C240
 Probe Info  		23.10  LDD1913  [9]
C311
 Probe Info  		6.28  LDD1978  [9]
C363
 Probe Info  		22.94  LDD2024  [9]
C388
 Probe Info  		40.50  LDD2047  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C175(1.43)  LDD1507  [10]
 LDCM0215  AC10 HEK-293T C196(1.00); C210(1.05)  LDD1508  [10]
 LDCM0226  AC11 HEK-293T C175(1.10); C210(1.09)  LDD1509  [10]
 LDCM0276  AC17 HEK-293T C175(1.55)  LDD1515  [10]
 LDCM0277  AC18 HEK-293T C196(1.07); C210(1.10)  LDD1516  [10]
 LDCM0278  AC19 HEK-293T C175(0.88); C210(0.83)  LDD1517  [10]
 LDCM0279  AC2 HEK-293T C196(1.05); C210(1.16)  LDD1518  [10]
 LDCM0285  AC25 HEK-293T C175(1.00)  LDD1524  [10]
 LDCM0286  AC26 HEK-293T C196(1.00); C210(0.90)  LDD1525  [10]
 LDCM0287  AC27 HEK-293T C175(0.95); C210(0.96)  LDD1526  [10]
 LDCM0290  AC3 HEK-293T C175(1.05); C210(1.04)  LDD1529  [10]
 LDCM0294  AC33 HEK-293T C175(1.20)  LDD1533  [10]
 LDCM0295  AC34 HEK-293T C196(0.99); C210(1.10)  LDD1534  [10]
 LDCM0296  AC35 HEK-293T C175(1.00); C210(1.07)  LDD1535  [10]
 LDCM0303  AC41 HEK-293T C175(1.02)  LDD1542  [10]
 LDCM0304  AC42 HEK-293T C196(1.04); C210(0.92)  LDD1543  [10]
 LDCM0305  AC43 HEK-293T C175(1.02); C210(1.02)  LDD1544  [10]
 LDCM0311  AC49 HEK-293T C175(1.07)  LDD1550  [10]
 LDCM0313  AC50 HEK-293T C196(1.08); C210(1.11)  LDD1552  [10]
 LDCM0314  AC51 HEK-293T C175(1.06); C210(0.99)  LDD1553  [10]
 LDCM0320  AC57 HEK-293T C175(1.02)  LDD1559  [10]
 LDCM0321  AC58 HEK-293T C196(0.99); C210(1.08)  LDD1560  [10]
 LDCM0322  AC59 HEK-293T C175(1.10); C210(0.96)  LDD1561  [10]
 LDCM0356  AKOS034007680 HEK-293T C175(1.09)  LDD1570  [10]
 LDCM0632  CL-Sc Hep-G2 C175(0.37)  LDD2227  [6]
 LDCM0369  CL100 HEK-293T C175(0.80)  LDD1573  [10]
 LDCM0371  CL102 HEK-293T C175(1.16)  LDD1575  [10]
 LDCM0373  CL104 HEK-293T C175(0.94)  LDD1577  [10]
 LDCM0375  CL106 HEK-293T C175(0.97)  LDD1579  [10]
 LDCM0377  CL108 HEK-293T C175(0.84)  LDD1581  [10]
 LDCM0380  CL110 HEK-293T C175(0.92)  LDD1584  [10]
 LDCM0382  CL112 HEK-293T C175(0.89)  LDD1586  [10]
 LDCM0384  CL114 HEK-293T C175(1.06)  LDD1588  [10]
 LDCM0386  CL116 HEK-293T C175(0.91)  LDD1590  [10]
 LDCM0388  CL118 HEK-293T C175(1.03)  LDD1592  [10]
 LDCM0391  CL120 HEK-293T C175(0.97)  LDD1595  [10]
 LDCM0393  CL122 HEK-293T C175(1.09)  LDD1597  [10]
 LDCM0395  CL124 HEK-293T C175(0.96)  LDD1599  [10]
 LDCM0397  CL126 HEK-293T C175(0.97)  LDD1601  [10]
 LDCM0399  CL128 HEK-293T C175(0.86)  LDD1603  [10]
 LDCM0401  CL14 HEK-293T C175(1.04)  LDD1605  [10]
 LDCM0403  CL16 HEK-293T C175(1.00)  LDD1607  [10]
 LDCM0404  CL17 HEK-293T C175(1.08)  LDD1608  [10]
 LDCM0405  CL18 HEK-293T C196(0.93); C210(1.04)  LDD1609  [10]
 LDCM0406  CL19 HEK-293T C175(1.15); C210(1.05)  LDD1610  [10]
 LDCM0407  CL2 HEK-293T C175(1.19)  LDD1611  [10]
 LDCM0414  CL26 HEK-293T C175(1.08)  LDD1618  [10]
 LDCM0416  CL28 HEK-293T C175(1.00)  LDD1620  [10]
 LDCM0417  CL29 HEK-293T C175(1.02)  LDD1621  [10]
 LDCM0419  CL30 HEK-293T C196(1.07); C210(0.95)  LDD1623  [10]
 LDCM0420  CL31 HEK-293T C175(1.03); C210(0.97)  LDD1624  [10]
 LDCM0429  CL4 HEK-293T C175(0.90)  LDD1633  [10]
 LDCM0430  CL40 HEK-293T C175(0.94)  LDD1634  [10]
 LDCM0431  CL41 HEK-293T C175(0.99)  LDD1635  [10]
 LDCM0432  CL42 HEK-293T C196(1.00); C210(1.06)  LDD1636  [10]
 LDCM0433  CL43 HEK-293T C175(1.10); C210(0.99)  LDD1637  [10]
 LDCM0440  CL5 HEK-293T C175(1.15)  LDD1644  [10]
 LDCM0441  CL50 HEK-293T C175(1.01)  LDD1645  [10]
 LDCM0443  CL52 HEK-293T C175(0.96)  LDD1646  [10]
 LDCM0444  CL53 HEK-293T C175(0.97)  LDD1647  [10]
 LDCM0445  CL54 HEK-293T C196(0.96); C210(1.00)  LDD1648  [10]
 LDCM0446  CL55 HEK-293T C175(1.08); C210(1.06)  LDD1649  [10]
 LDCM0451  CL6 HEK-293T C196(0.94); C210(1.02)  LDD1654  [10]
 LDCM0454  CL62 HEK-293T C175(1.06)  LDD1657  [10]
 LDCM0456  CL64 HEK-293T C175(0.94)  LDD1659  [10]
 LDCM0457  CL65 HEK-293T C175(1.13)  LDD1660  [10]
 LDCM0458  CL66 HEK-293T C196(0.93); C210(0.99)  LDD1661  [10]
 LDCM0459  CL67 HEK-293T C175(1.08); C210(0.95)  LDD1662  [10]
 LDCM0462  CL7 HEK-293T C175(1.04); C210(1.06)  LDD1665  [10]
 LDCM0467  CL74 HEK-293T C175(1.16)  LDD1670  [10]
 LDCM0469  CL76 HEK-293T C175(1.02)  LDD1672  [10]
 LDCM0470  CL77 HEK-293T C175(0.88)  LDD1673  [10]
 LDCM0471  CL78 HEK-293T C196(0.95); C210(1.06)  LDD1674  [10]
 LDCM0472  CL79 HEK-293T C175(0.99); C210(0.93)  LDD1675  [10]
 LDCM0480  CL86 HEK-293T C175(1.05)  LDD1683  [10]
 LDCM0482  CL88 HEK-293T C175(0.90)  LDD1685  [10]
 LDCM0483  CL89 HEK-293T C175(1.18)  LDD1686  [10]
 LDCM0485  CL90 HEK-293T C196(1.04); C210(0.78)  LDD1688  [10]
 LDCM0486  CL91 HEK-293T C175(1.08); C210(1.06)  LDD1689  [10]
 LDCM0493  CL98 HEK-293T C175(1.02)  LDD1696  [10]
 LDCM0625  F8 Ramos C175(1.14)  LDD2187  [11]
 LDCM0572  Fragment10 Ramos C175(0.83)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C175(6.60)  LDD2190  [11]
 LDCM0574  Fragment12 Ramos C175(0.85)  LDD2191  [11]
 LDCM0575  Fragment13 Ramos C175(1.10)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C175(0.90)  LDD2193  [11]
 LDCM0579  Fragment20 Ramos C175(0.97)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C175(1.23)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C175(3.81)  LDD2196  [11]
 LDCM0578  Fragment27 Ramos C175(0.93)  LDD2197  [11]
 LDCM0586  Fragment28 Ramos C175(0.69)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C175(0.95)  LDD2199  [11]
 LDCM0589  Fragment31 Ramos C175(1.03)  LDD2200  [11]
 LDCM0590  Fragment32 Ramos C175(1.04)  LDD2201  [11]
 LDCM0468  Fragment33 Ramos C175(1.03)  LDD2202  [11]
 LDCM0596  Fragment38 Ramos C175(0.83)  LDD2203  [11]
 LDCM0566  Fragment4 Ramos C175(0.93)  LDD2184  [11]
 LDCM0427  Fragment51 HEK-293T C175(1.10)  LDD1631  [10]
 LDCM0610  Fragment52 Ramos C175(1.49)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C175(1.08)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C175(0.58)  LDD2186  [11]
 LDCM0116  HHS-0101 DM93 Y67(0.78)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y67(0.23)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y67(0.06)  LDD0266  [3]
 LDCM0120  HHS-0701 DM93 Y67(0.57)  LDD0268  [3]
 LDCM0022  KB02 Ramos C175(0.45)  LDD2182  [11]
 LDCM0023  KB03 Ramos C175(0.60)  LDD2183  [11]
 LDCM0024  KB05 G361 C175(3.11)  LDD3311  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-36 receptor antagonist protein (IL36RN) IL-1 family Q9UBH0

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578