Details of the Target

General Information of Target

Target ID LDTP00887
Target Name Calumenin (CALU)
Gene Name CALU
Gene ID 813
Synonyms
Calumenin; Crocalbin; IEF SSP 9302
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKT
FDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNE
DGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFL
HPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEF
RDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGS
QATDFGEALVRHDEF
Target Bioclass
Other
Family
CREC family
Subcellular location
Endoplasmic reticulum membrane
Function Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
Uniprot ID
O43852
Ensemble ID
ENST00000249364.9
HGNC ID
HGNC:1458

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.F87C .
CCK81 SNV: p.N40I .
IGROV1 SNV: p.R159M .
KYSE30 SNV: p.D46N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		5.94  LDD0318  [1]
SAA-alkyne
 Probe Info  		1.09  LDD0252  [2]
TH211
 Probe Info  		Y106(20.00); Y263(7.53)  LDD0257  [3]
TH216
 Probe Info  		Y106(20.00)  LDD0259  [3]
ONAyne
 Probe Info  		K70(3.16); K75(0.88)  LDD0274  [4]
OPA-S-S-alkyne
 Probe Info  		K70(0.19)  LDD3494  [5]
Probe 1
 Probe Info  		Y106(12.71); Y185(10.70)  LDD3495  [6]
HHS-482
 Probe Info  		Y106(0.88); Y263(0.85); Y275(0.97); Y47(0.72)  LDD0285  [7]
HHS-475
 Probe Info  		Y263(0.71); Y106(0.74); Y275(0.81); Y47(0.84)  LDD0264  [8]
HHS-465
 Probe Info  		Y106(10.00); Y275(10.00); Y294(9.67)  LDD2237  [9]
5E-2FA
 Probe Info  		H30(0.00); H29(0.00)  LDD2235  [10]
ATP probe
 Probe Info  		K281(0.00); K37(0.00); K284(0.00); K79(0.00)  LDD0199  [11]
m-APA
 Probe Info  		H181(0.00); H116(0.00); H30(0.00); H29(0.00)  LDD2231  [10]
1d-yne
 Probe Info  		N.A.  LDD0358  [12]
SF
 Probe Info  		N.A.  LDD0028  [13]
STPyne
 Probe Info  		N.A.  LDD0009  [14]
Ox-W18
 Probe Info  		N.A.  LDD2175  [15]
1c-yne
 Probe Info  		K293(0.00); K287(0.00); K173(0.00); K255(0.00)  LDD0228  [12]
Acrolein
 Probe Info  		H39(0.00); H272(0.00); H116(0.00); H181(0.00)  LDD0217  [16]
Crotonaldehyde
 Probe Info  		H272(0.00); H265(0.00); H29(0.00)  LDD0219  [16]
PAL-AfBPP Probe
Click To Hide/Show 92 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ILS-1 PP
 Probe Info  		2.21  LDD0417  [17]
C003
 Probe Info  		13.83  LDD1713  [18]
C017
 Probe Info  		17.75  LDD1725  [18]
C022
 Probe Info  		7.01  LDD1728  [18]
C040
 Probe Info  		8.57  LDD1740  [18]
C041
 Probe Info  		6.77  LDD1741  [18]
C052
 Probe Info  		5.74  LDD1750  [18]
C063
 Probe Info  		17.63  LDD1760  [18]
C085
 Probe Info  		5.10  LDD1777  [18]
C087
 Probe Info  		21.41  LDD1779  [18]
C106
 Probe Info  		22.78  LDD1793  [18]
C108
 Probe Info  		9.58  LDD1795  [18]
C112
 Probe Info  		23.75  LDD1799  [18]
C131
 Probe Info  		13.00  LDD1813  [18]
C145
 Probe Info  		9.00  LDD1827  [18]
C161
 Probe Info  		10.41  LDD1841  [18]
C165
 Probe Info  		18.38  LDD1845  [18]
C166
 Probe Info  		7.57  LDD1846  [18]
C170
 Probe Info  		14.93  LDD1850  [18]
C174
 Probe Info  		5.13  LDD1854  [18]
C186
 Probe Info  		9.99  LDD1864  [18]
C187
 Probe Info  		20.68  LDD1865  [18]
C191
 Probe Info  		16.56  LDD1868  [18]
C193
 Probe Info  		5.13  LDD1869  [18]
C197
 Probe Info  		5.46  LDD1873  [18]
C201
 Probe Info  		39.40  LDD1877  [18]
C203
 Probe Info  		7.62  LDD1878  [18]
C206
 Probe Info  		23.10  LDD1881  [18]
C208
 Probe Info  		5.06  LDD1883  [18]
C210
 Probe Info  		60.13  LDD1884  [18]
C211
 Probe Info  		10.13  LDD1885  [18]
C213
 Probe Info  		20.11  LDD1887  [18]
C214
 Probe Info  		8.00  LDD1888  [18]
C218
 Probe Info  		16.11  LDD1892  [18]
C220
 Probe Info  		11.71  LDD1894  [18]
C228
 Probe Info  		15.24  LDD1901  [18]
C231
 Probe Info  		11.79  LDD1904  [18]
C232
 Probe Info  		77.17  LDD1905  [18]
C233
 Probe Info  		11.96  LDD1906  [18]
C234
 Probe Info  		6.36  LDD1907  [18]
C235
 Probe Info  		22.16  LDD1908  [18]
C244
 Probe Info  		16.00  LDD1917  [18]
C249
 Probe Info  		17.27  LDD1922  [18]
C265
 Probe Info  		14.52  LDD1936  [18]
C270
 Probe Info  		6.45  LDD1940  [18]
C284
 Probe Info  		26.17  LDD1954  [18]
C293
 Probe Info  		17.03  LDD1963  [18]
C296
 Probe Info  		14.83  LDD1966  [18]
C302
 Probe Info  		6.06  LDD1971  [18]
C305
 Probe Info  		10.48  LDD1974  [18]
C310
 Probe Info  		24.42  LDD1977  [18]
C313
 Probe Info  		17.75  LDD1980  [18]
C314
 Probe Info  		12.21  LDD1981  [18]
C317
 Probe Info  		5.98  LDD1983  [18]
C322
 Probe Info  		7.16  LDD1988  [18]
C326
 Probe Info  		9.38  LDD1990  [18]
C338
 Probe Info  		59.71  LDD2001  [18]
C346
 Probe Info  		11.16  LDD2007  [18]
C348
 Probe Info  		15.78  LDD2009  [18]
C349
 Probe Info  		10.78  LDD2010  [18]
C350
 Probe Info  		29.04  LDD2011  [18]
C353
 Probe Info  		10.13  LDD2014  [18]
C354
 Probe Info  		8.51  LDD2015  [18]
C355
 Probe Info  		24.25  LDD2016  [18]
C363
 Probe Info  		16.56  LDD2024  [18]
C364
 Probe Info  		16.56  LDD2025  [18]
C382
 Probe Info  		10.41  LDD2041  [18]
C383
 Probe Info  		20.11  LDD2042  [18]
C390
 Probe Info  		22.94  LDD2049  [18]
C391
 Probe Info  		21.71  LDD2050  [18]
C397
 Probe Info  		12.04  LDD2056  [18]
C403
 Probe Info  		15.03  LDD2061  [18]
C407
 Probe Info  		19.43  LDD2064  [18]
C413
 Probe Info  		11.08  LDD2069  [18]
C424
 Probe Info  		7.57  LDD2079  [18]
C429
 Probe Info  		15.35  LDD2084  [18]
C430
 Probe Info  		4.96  LDD2085  [18]
C431
 Probe Info  		19.97  LDD2086  [18]
C433
 Probe Info  		5.86  LDD2088  [18]
FFF probe11
 Probe Info  		13.00  LDD0471  [19]
FFF probe12
 Probe Info  		18.68  LDD0473  [19]
FFF probe13
 Probe Info  		20.00  LDD0475  [19]
FFF probe14
 Probe Info  		20.00  LDD0477  [19]
FFF probe15
 Probe Info  		14.80  LDD0478  [19]
FFF probe2
 Probe Info  		20.00  LDD0463  [19]
FFF probe3
 Probe Info  		20.00  LDD0464  [19]
FFF probe4
 Probe Info  		20.00  LDD0466  [19]
FFF probe6
 Probe Info  		20.00  LDD0467  [19]
FFF probe7
 Probe Info  		20.00  LDD0483  [19]
FFF probe9
 Probe Info  		20.00  LDD0470  [19]
JN0003
 Probe Info  		20.00  LDD0469  [19]
STS-1
 Probe Info  		N.A.  LDD0136  [20]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H272(0.00); H116(0.00); H49(0.00); H265(0.00)  LDD0222  [16]
 LDCM0116  HHS-0101 DM93 Y263(0.71); Y106(0.74); Y275(0.81); Y47(0.84)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y263(0.60); Y106(0.62); Y275(0.70); Y47(0.73)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y263(0.64); Y275(0.73); Y185(0.74); Y106(0.77)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y106(0.72); Y47(0.79); Y275(0.82); Y263(0.84)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y275(0.39); Y106(0.47); Y263(0.70); Y47(0.97)  LDD0268  [8]
 LDCM0107  IAA HeLa H39(0.00); H116(0.00); H181(0.00); H272(0.00)  LDD0221  [16]
 LDCM0123  JWB131 DM93 Y106(0.88); Y263(0.85); Y275(0.97); Y47(0.72)  LDD0285  [7]
 LDCM0124  JWB142 DM93 Y106(0.55); Y263(0.61); Y275(0.68); Y47(0.63)  LDD0286  [7]
 LDCM0125  JWB146 DM93 Y106(1.00); Y263(0.90); Y275(0.94); Y47(0.99)  LDD0287  [7]
 LDCM0126  JWB150 DM93 Y106(2.64); Y263(2.52); Y275(2.82); Y47(3.25)  LDD0288  [7]
 LDCM0127  JWB152 DM93 Y106(1.69); Y263(1.62); Y275(2.02); Y47(2.29)  LDD0289  [7]
 LDCM0128  JWB198 DM93 Y106(1.10); Y263(0.82); Y275(0.96); Y47(0.99)  LDD0290  [7]
 LDCM0129  JWB202 DM93 Y106(0.44); Y263(0.46); Y275(0.61); Y47(0.43)  LDD0291  [7]
 LDCM0130  JWB211 DM93 Y106(0.86); Y263(0.68); Y275(0.91); Y47(0.86)  LDD0292  [7]
 LDCM0109  NEM HeLa H116(0.00); H265(0.00); H272(0.00); H181(0.00)  LDD0223  [16]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cystic fibrosis transmembrane conductance regulator (CFTR) ABCC family P13569
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
11 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
14 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
15 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
16 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
17 A Chemical Proteomic Analysis of Illudin-Interacting Proteins. Chemistry. 2019 Sep 25;25(54):12644-12651. doi: 10.1002/chem.201902919. Epub 2019 Sep 3.
Mass spectrometry data entry: PXD014175
18 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
19 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
20 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.