Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C017  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 105 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12253  Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) 34.8
 LDTP11127  ADP-dependent glucokinase (ADPGK) 27.5
 LDTP03394  Ceramide synthase 1 (CERS1) 25.6
 LDTP04689  Adenosine kinase (ADK) 23.8
 LDTP12720  Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) 20.5
 LDTP01803  Adenosine deaminase (ADA) 18.9
 LDTP06750  Prolyl 3-hydroxylase 1 (P3H1) 18.9
 LDTP08790  Mitochondrial cardiolipin hydrolase (PLD6) 16.0
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 14.0
 LDTP05588  RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) 13.4
 LDTP13240  Poly [ADP-ribose] polymerase 2 (PARP2) 12.8
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 12.7
 LDTP01462  Endonuclease domain-containing 1 protein (ENDOD1) 12.7
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 12.7
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 12.6
 LDTP07003  Presequence protease, mitochondrial (PITRM1) 12.6
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 11.7
 LDTP12444  Xaa-Pro aminopeptidase 1 (XPNPEP1) 11.7
 LDTP11336  Chitinase domain-containing protein 1 (CHID1) 11.6
 LDTP02188  Protein disulfide-isomerase (P4HB) 11.6
 LDTP09056  Inactive C-alpha-formylglycine-generating enzyme 2 (SUMF2) 11.1
 LDTP09031  Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (POMGNT2) 11.0
 LDTP02679  Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) 10.9
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 10.6
 LDTP13862  GDP-fucose protein O-fucosyltransferase 2 (POFUT2) 10.1
 LDTP10329  Ceramide synthase 2 (CERS2) 9.9
 LDTP03418  Proteasome subunit beta type-6 (PSMB6) 9.9
 LDTP17247  5'-nucleotidase domain-containing protein 1 (NT5DC1) 9.8
 LDTP06546  Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform (PPP2R5E) 9.4
 LDTP08209  Acyl-coenzyme A thioesterase 1 (ACOT1) 9.4
 LDTP01065  E3 ubiquitin-protein ligase TRIM13 (TRIM13) 9.4
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 9.3
 LDTP12610  Obg-like ATPase 1 (OLA1) 9.2
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 9.1
 LDTP13620  Lysosomal thioesterase PPT2 (PPT2) 9.1
 LDTP08532  Inositol 1,4,5-trisphosphate receptor-interacting protein (ITPRIP) 9.1
 LDTP02848  NADPH--cytochrome P450 reductase (POR) 8.8
 LDTP05773  Peroxiredoxin-4 (PRDX4) 8.6
 LDTP03842  Squalene synthase (FDFT1) 8.5
 LDTP10101  Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10) 8.4
 LDTP09925  COP9 signalosome complex subunit 5 (COPS5) 8.3
 LDTP11437  Peroxisomal trans-2-enoyl-CoA reductase (PECR) 8.1
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 8.1
 LDTP03854  Vitamin K-dependent gamma-carboxylase (GGCX) 8.1
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 7.9
 LDTP11215  Plasma alpha-L-fucosidase (FUCA2) 7.9
 LDTP02150  ATP synthase subunit beta, mitochondrial (ATP5F1B) 7.7
 LDTP03540  Heme oxygenase 2 (HMOX2) 7.7
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 7.7
 LDTP04211  Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) 7.7
 LDTP01300  Cartilage-associated protein (CRTAP) 7.6
 LDTP12020  Histone-lysine N-methyltransferase SMYD3 (SMYD3) 7.6
 LDTP07503  Endoplasmic reticulum aminopeptidase 2 (ERAP2) 7.5
 LDTP04183  Lanosterol synthase (LSS) 7.5
 LDTP06145  Protein disulfide-isomerase A5 (PDIA5) 7.5
 LDTP09874  Endoplasmic reticulum protein SC65 (P3H4) 7.4
 LDTP13751  Exonuclease 1 (EXO1) 7.3
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 7.3
 LDTP03220  NAD-dependent malic enzyme, mitochondrial (ME2) 7.1
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 7.1
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 7.0
 LDTP11273  N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) 7.0
 LDTP08590  DnaJ homolog subfamily C member 10 (DNAJC10) 7.0
 LDTP18123  Isochorismatase domain-containing protein 2 (ISOC2) 7.0
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 7.0
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 6.9
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 6.8
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 6.8
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 6.8
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 6.7
 LDTP11216  Dehydrogenase/reductase SDR family member 4 (DHRS4) 6.7
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 6.7
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 6.7
 LDTP11494  Neurolysin, mitochondrial (NLN) 6.7
 LDTP08017  Endoplasmic reticulum metallopeptidase 1 (ERMP1) 6.6
 LDTP06839  NAD kinase 2, mitochondrial (NADK2) 6.5
 LDTP05920  Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G) 6.5
 LDTP04120  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A) 6.5
 LDTP02713  Macrophage migration inhibitory factor (MIF) 6.5
 LDTP02260  Beta-glucuronidase (GUSB) 6.4
 LDTP13220  Phosphatidylserine decarboxylase proenzyme, mitochondrial (PISD) 6.4
 LDTP12040  Prostaglandin E synthase 2 (PTGES2) 6.4
 LDTP11178  Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1) 6.4
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 6.4
 LDTP13491  E3 ubiquitin-protein ligase AMFR (AMFR) 6.2
 LDTP18414  5'-nucleotidase domain-containing protein 2 (NT5DC2) 6.2
 LDTP03417  Proteasome subunit beta type-4 (PSMB4) 6.2
 LDTP00697  Cytochrome b5 type B (CYB5B) 6.1
 LDTP02359  Heme oxygenase 1 (HMOX1) 6.1
 LDTP00575  Glutathione S-transferase A4 (GSTA4) 6.1
 LDTP12588  Isoleucine--tRNA ligase, mitochondrial (IARS2) 6.1
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 6.1
 LDTP08859  Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4) 6.1
 LDTP16025  Ketosamine-3-kinase (FN3KRP) 6.0
 LDTP09835  GPI-anchor transamidase (PIGK) 5.9
 LDTP08401  All trans-polyprenyl-diphosphate synthase PDSS2 (PDSS2) 5.9
 LDTP01687  Apoptosis-inducing factor 1, mitochondrial (AIFM1) 5.9
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 5.9
 LDTP00645  Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) 5.9
 LDTP14278  Sulfide:quinone oxidoreductase, mitochondrial (SQOR) 5.7
 LDTP06977  GPI ethanolamine phosphate transferase 2 (PIGG) 5.7
 LDTP00482  Peripheral plasma membrane protein CASK (CASK) 5.7
 LDTP05624  Polypeptide N-acetylgalactosaminyltransferase 1 (GALNT1) 5.6
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 5.6
 LDTP07868  Protein O-mannosyl-transferase TMTC3 (TMTC3) 5.5
Transporter and channel
Click to Show/Hide to Show 49 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 53.8
 LDTP07667  Transmembrane protein 205 (TMEM205) 53.1
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 37.5
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 19.3
 LDTP08881  Transmembrane protein 199 (TMEM199) 18.9
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 18.3
 LDTP09278  Multiple coagulation factor deficiency protein 2 (MCFD2) 14.8
 LDTP20002  Transmembrane protein 160 (TMEM160) 14.1
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 13.2
 LDTP15648  BRI3-binding protein (BRI3BP) 12.0
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 11.7
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 11.5
 LDTP09180  Zinc transporter 7 (SLC30A7) 10.6
 LDTP00592  Surfeit locus protein 4 (SURF4) 10.1
 LDTP07156  Protein wntless homolog (WLS) 8.9
 LDTP11927  Essential MCU regulator, mitochondrial (SMDT1) 8.3
 LDTP05384  Mitochondrial 2-oxoglutarate/malate carrier protein (SLC25A11) 8.2
 LDTP16190  Protein FAM8A1 (FAM8A1) 8.1
 LDTP05871  Protein OS-9 (OS9) 7.8
 LDTP07236  Protein GPR107 (GPR107) 7.7
 LDTP14682  Translocon-associated protein subunit beta (SSR2) 7.7
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 7.6
 LDTP11116  45 kDa calcium-binding protein (SDF4) 7.5
 LDTP03546  Translocator protein (TSPO) 7.5
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 7.4
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 7.4
 LDTP03405  Calnexin (CANX) 7.2
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 7.1
 LDTP11245  Derlin-1 (DERL1) 7.1
 LDTP00262  Acetyl-coenzyme A transporter 1 (SLC33A1) 7.0
 LDTP09949  Transportin-1 (TNPO1) 7.0
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 6.7
 LDTP13164  Protein sel-1 homolog 1 (SEL1L) 6.7
 LDTP15948  Transmembrane protein 126A (TMEM126A) 6.6
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 6.5
 LDTP10669  Melanoma inhibitory activity protein 2 (MIA2) 6.5
 LDTP13256  SUN domain-containing protein 2 (SUN2) 6.3
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 6.2
 LDTP09288  Vang-like protein 1 (VANGL1) 6.1
 LDTP11213  Nucleoporin NDC1 (NDC1) 6.1
 LDTP10059  PAT complex subunit CCDC47 (CCDC47) 5.9
 LDTP04462  H(+)/Cl(-) exchange transporter 6 (CLCN6) 5.9
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 5.9
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 5.8
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 5.7
 LDTP10343  Vacuole membrane protein 1 (VMP1) 5.6
 LDTP11291  Transmembrane emp24 domain-containing protein 9 (TMED9) 5.6
 LDTP03952  Reduced folate transporter (SLC19A1) 5.5
 LDTP10533  Sorting nexin-27 (SNX27) 5.5
GPCR
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15303  Membrane progestin receptor alpha (PAQR7) 10.3
Immunoglobulin
Click to Show/Hide to Show 2 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP03538  HLA class I histocompatibility antigen, alpha chain F (HLA-F) 24.8
 LDTP02491  HLA class I histocompatibility antigen, C alpha chain (HLA-C) 10.7
Other
Click to Show/Hide to Show 59 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15623  Neuferricin (CYB5D2) 19.2
 LDTP10184  HAUS augmin-like complex subunit 1 (HAUS1) 18.1
 LDTP00887  Calumenin (CALU) 17.8
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 16.0
 LDTP06097  Reticulocalbin-2 (RCN2) 15.1
 LDTP13630  Neudesin (NENF) 15.0
 LDTP15634  Ubiquinol-cytochrome c reductase complex assembly factor 5 (UQCC5) 14.6
 LDTP14939  Membralin (TMEM259) 13.4
 LDTP14458  Tetraspanin-6 (TSPAN6) 12.9
 LDTP05160  Nucleobindin-2 (NUCB2) 11.2
 LDTP11239  Protein misato homolog 1 (MSTO1) 11.1
 LDTP13606  Mammalian ependymin-related protein 1 (EPDR1) 10.2
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 10.1
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 9.8
 LDTP13086  Syntaxin-18 (STX18) 9.6
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 9.3
 LDTP01494  Protein YIF1A (YIF1A) 9.1
 LDTP07891  Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP) 8.9
 LDTP05375  Nucleobindin-1 (NUCB1) 8.4
 LDTP10775  Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) 8.3
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 8.1
 LDTP08224  Protein LYRIC (MTDH) 8.0
 LDTP05089  Immunoglobulin-binding protein 1 (IGBP1) 7.9
 LDTP08606  Nurim (NRM) 7.9
 LDTP17003  Matrix-remodeling-associated protein 7 (MXRA7) 7.7
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 7.7
 LDTP11978  HAUS augmin-like complex subunit 4 (HAUS4) 7.6
 LDTP15434  ADP-ribosylation factor-like protein 6-interacting protein 6 (ARL6IP6) 7.5
 LDTP08573  Ankyrin repeat and KH domain-containing protein 1 (ANKHD1) 7.5
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 7.4
 LDTP03968  Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO) 7.3
 LDTP09297  Large ribosomal subunit protein mL64 (GADD45GIP1) 7.3
 LDTP15110  Protein C3orf33 (C3orf33) 7.0
 LDTP03545  Alpha-2-macroglobulin receptor-associated protein (LRPAP1) 6.9
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 6.8
 LDTP16080  Protein FAM114A2 (FAM114A2) 6.7
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 6.6
 LDTP06948  Protein YIF1B (YIF1B) 6.6
 LDTP10056  Protein FAM162A (FAM162A) 6.5
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 6.3
 LDTP11979  Coiled-coil domain-containing protein 134 (CCDC134) 6.2
 LDTP05789  DnaJ homolog subfamily C member 3 (DNAJC3) 6.2
 LDTP10924  Prohibitin-2 (PHB2) 6.2
 LDTP01608  Proteasome assembly chaperone 1 (PSMG1) 6.2
 LDTP13982  Mitochondrial fission 1 protein (FIS1) 6.2
 LDTP02124  Keratin, type I cytoskeletal 18 (KRT18) 6.1
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 6.1
 LDTP02734  Endoplasmin (HSP90B1) 6.0
 LDTP11113  Receptor expression-enhancing protein 2 (REEP2) 5.9
 LDTP15095  Receptor expression-enhancing protein 3 (REEP3) 5.9
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 5.9
 LDTP16285  Transducin beta-like protein 2 (TBL2) 5.9
 LDTP02230  Laminin subunit beta-1 (LAMB1) 5.9
 LDTP06373  Mitochondrial import receptor subunit TOM20 homolog (TOMM20) 5.8
 LDTP02297  Vimentin (VIM) 5.8
 LDTP01938  Apolipoprotein C-I (APOC1) 5.8
 LDTP15714  Mdm2-binding protein (MTBP) 5.8
 LDTP06352  Reticulocalbin-1 (RCN1) 5.8
 LDTP06072  Dedicator of cytokinesis protein 1 (DOCK1) 5.6

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587