Details of the Target

General Information of Target

Target ID LDTP03545
Target Name Alpha-2-macroglobulin receptor-associated protein (LRPAP1)
Gene Name LRPAP1
Gene ID 4043
Synonyms
A2MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; Low density lipoprotein receptor-related protein-associated protein 1; RAP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLN
QLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILA
KYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHK
EKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRR
VSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEI
AHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL
Target Bioclass
Other
Family
Alpha-2-MRAP family
Subcellular location
Rough endoplasmic reticulum lumen
Function Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
Uniprot ID
P30533
Ensemble ID
ENST00000650182.1
HGNC ID
HGNC:6701

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
SAA-alkyne
 Probe Info  		1.27  LDD0252  [1]
TH211
 Probe Info  		Y294(20.00)  LDD0260  [2]
ONAyne
 Probe Info  		K304(5.88)  LDD0275  [3]
OPA-S-S-alkyne
 Probe Info  		K182(0.73); K340(1.47); K107(1.78); K323(2.24)  LDD3494  [4]
Probe 1
 Probe Info  		Y246(12.73); Y336(72.13)  LDD3495  [5]
HHS-475
 Probe Info  		Y336(0.94); Y294(1.11)  LDD0264  [6]
HHS-465
 Probe Info  		Y294(6.70)  LDD2237  [7]
5E-2FA
 Probe Info  		H184(0.00); H341(0.00); H243(0.00)  LDD2235  [8]
ATP probe
 Probe Info  		K287(0.00); K182(0.00); K323(0.00); K65(0.00)  LDD0199  [9]
m-APA
 Probe Info  		H184(0.00); H243(0.00); H341(0.00)  LDD2231  [8]
ATP probe
 Probe Info  		N.A.  LDD0035  [10]
SF
 Probe Info  		N.A.  LDD0028  [11]
STPyne
 Probe Info  		N.A.  LDD0009  [12]
Acrolein
 Probe Info  		H243(0.00); H184(0.00); H341(0.00); H307(0.00)  LDD0217  [13]
Crotonaldehyde
 Probe Info  		H307(0.00); H243(0.00)  LDD0219  [13]
Methacrolein
 Probe Info  		N.A.  LDD0218  [13]
AOyne
 Probe Info  		14.50  LDD0443  [14]
HHS-482
 Probe Info  		Y294(0.60)  LDD2239  [7]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C017
 Probe Info  		6.87  LDD1725  [15]
C052
 Probe Info  		5.74  LDD1750  [15]
C158
 Probe Info  		11.39  LDD1838  [15]
C187
 Probe Info  		15.56  LDD1865  [15]
C191
 Probe Info  		10.06  LDD1868  [15]
C207
 Probe Info  		18.13  LDD1882  [15]
C338
 Probe Info  		10.70  LDD2001  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H243(0.00); H184(0.00); H341(0.00); H307(0.00)  LDD0222  [13]
 LDCM0116  HHS-0101 DM93 Y336(0.94); Y294(1.11)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y336(0.66); Y294(0.76)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y294(0.50); Y336(9.07)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y294(0.84); Y336(4.27)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y294(0.62); Y336(12.96)  LDD0268  [6]
 LDCM0107  IAA HeLa H243(0.00); H184(0.00); H307(0.00); H341(0.00)  LDD0221  [13]
 LDCM0109  NEM HeLa H243(0.00); H184(0.00); H307(0.00); H341(0.00)  LDD0223  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Low-density lipoprotein receptor (LDLR) LDLR family P01130
Prolow-density lipoprotein receptor-related protein 1 (LRP1) LDLR family Q07954
Sortilin-related receptor (SORL1) VPS10-related sortilin family Q92673
Sortilin (SORT1) VPS10-related sortilin family Q99523
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Very low-density lipoprotein receptor (VLDLR) . P98155

References

1 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587