Details of the Target

General Information of Target

Target ID LDTP09056
Target Name Inactive C-alpha-formylglycine-generating enzyme 2 (SUMF2)
Gene Name SUMF2
Gene ID 25870
Synonyms
Inactive C-alpha-formylglycine-generating enzyme 2; Paralog of formylglycine-generating enzyme; pFGE; Sulfatase-modifying factor 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARHGLPLLPLLSLLVGAWLKLGNGQATSMVQLQGGRFLMGTNSPDSRDGDGPVREATVK
PFAIDIFPVTNKDFRDFVREKKYRTEAEMFGWSFVFEDFVSDELRNKATQPMKSVLWWLP
VEKAFWRQPAGPGSGIRERLEHPVLHVSWNDARAYCAWRGKRLPTEEEWEFAARGGLKGQ
VYPWGNWFQPNRTNLWQGKFPKGDKAEDGFHGVSPVNAFPAQNNYGLYDLLGNVWEWTAS
PYQAAEQDMRVLRGASWIDTADGSANHRARVTTRMGNTPDSASDNLGFRCAADAGRPPGE
L
Target Bioclass
Enzyme
Family
Sulfatase-modifying factor family
Subcellular location
Endoplasmic reticulum lumen
Function Lacks formylglycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.
Uniprot ID
Q8NBJ7
Ensemble ID
ENST00000275607.13
HGNC ID
HGNC:20415

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
NCIH196 SNV: p.P129L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K107(7.69); K113(10.00); K199(10.00); K60(6.94)  LDD0277  [1]
DBIA
 Probe Info  		C68(2.55)  LDD3316  [2]
BTD
 Probe Info  		C156(0.97)  LDD2123  [3]
Acrolein
 Probe Info  		N.A.  LDD0223  [4]
5E-2FA
 Probe Info  		H142(0.00); H267(0.00)  LDD2235  [5]
m-APA
 Probe Info  		H142(0.00); H146(0.00)  LDD2231  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
IPM
 Probe Info  		N.A.  LDD0025  [7]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [7]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [4]
Methacrolein
 Probe Info  		N.A.  LDD0218  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]
HHS-482
 Probe Info  		Y242(0.25)  LDD2239  [9]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C017
 Probe Info  		11.08  LDD1725  [10]
C026
 Probe Info  		8.46  LDD1732  [10]
C092
 Probe Info  		23.75  LDD1783  [10]
C153
 Probe Info  		14.72  LDD1834  [10]
C178
 Probe Info  		15.45  LDD1857  [10]
C388
 Probe Info  		42.52  LDD2047  [10]
C389
 Probe Info  		6.50  LDD2048  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C290(1.10)  LDD1509  [11]
 LDCM0278  AC19 HEK-293T C290(1.52)  LDD1517  [11]
 LDCM0284  AC24 HEK-293T C290(0.99)  LDD1523  [11]
 LDCM0287  AC27 HEK-293T C290(1.08)  LDD1526  [11]
 LDCM0290  AC3 HEK-293T C290(1.03)  LDD1529  [11]
 LDCM0293  AC32 HEK-293T C290(0.95)  LDD1532  [11]
 LDCM0296  AC35 HEK-293T C290(1.05)  LDD1535  [11]
 LDCM0302  AC40 HEK-293T C290(1.03)  LDD1541  [11]
 LDCM0305  AC43 HEK-293T C290(1.09)  LDD1544  [11]
 LDCM0310  AC48 HEK-293T C290(1.04)  LDD1549  [11]
 LDCM0314  AC51 HEK-293T C290(1.00)  LDD1553  [11]
 LDCM0319  AC56 HEK-293T C290(0.99)  LDD1558  [11]
 LDCM0322  AC59 HEK-293T C290(1.08)  LDD1561  [11]
 LDCM0328  AC64 HEK-293T C290(1.00)  LDD1567  [11]
 LDCM0345  AC8 HEK-293T C290(0.98)  LDD1569  [11]
 LDCM0275  AKOS034007705 HEK-293T C290(1.01)  LDD1514  [11]
 LDCM0369  CL100 HEK-293T C290(1.12)  LDD1573  [11]
 LDCM0373  CL104 HEK-293T C290(1.03)  LDD1577  [11]
 LDCM0377  CL108 HEK-293T C290(0.98)  LDD1581  [11]
 LDCM0382  CL112 HEK-293T C290(1.03)  LDD1586  [11]
 LDCM0386  CL116 HEK-293T C290(1.04)  LDD1590  [11]
 LDCM0390  CL12 HEK-293T C290(1.09)  LDD1594  [11]
 LDCM0391  CL120 HEK-293T C290(1.07)  LDD1595  [11]
 LDCM0395  CL124 HEK-293T C290(1.05)  LDD1599  [11]
 LDCM0399  CL128 HEK-293T C290(1.05)  LDD1603  [11]
 LDCM0403  CL16 HEK-293T C290(1.07)  LDD1607  [11]
 LDCM0406  CL19 HEK-293T C290(1.09)  LDD1610  [11]
 LDCM0412  CL24 HEK-293T C290(1.04)  LDD1616  [11]
 LDCM0416  CL28 HEK-293T C290(1.00)  LDD1620  [11]
 LDCM0420  CL31 HEK-293T C290(1.11)  LDD1624  [11]
 LDCM0425  CL36 HEK-293T C290(0.97)  LDD1629  [11]
 LDCM0429  CL4 HEK-293T C290(1.13)  LDD1633  [11]
 LDCM0430  CL40 HEK-293T C290(1.06)  LDD1634  [11]
 LDCM0433  CL43 HEK-293T C290(0.98)  LDD1637  [11]
 LDCM0438  CL48 HEK-293T C290(1.00)  LDD1642  [11]
 LDCM0443  CL52 HEK-293T C290(1.09)  LDD1646  [11]
 LDCM0446  CL55 HEK-293T C290(1.00)  LDD1649  [11]
 LDCM0452  CL60 HEK-293T C290(1.13)  LDD1655  [11]
 LDCM0456  CL64 HEK-293T C290(1.02)  LDD1659  [11]
 LDCM0459  CL67 HEK-293T C290(1.16)  LDD1662  [11]
 LDCM0462  CL7 HEK-293T C290(1.01)  LDD1665  [11]
 LDCM0465  CL72 HEK-293T C290(1.01)  LDD1668  [11]
 LDCM0469  CL76 HEK-293T C290(1.09)  LDD1672  [11]
 LDCM0472  CL79 HEK-293T C290(1.19)  LDD1675  [11]
 LDCM0478  CL84 HEK-293T C290(1.02)  LDD1681  [11]
 LDCM0482  CL88 HEK-293T C290(1.03)  LDD1685  [11]
 LDCM0486  CL91 HEK-293T C290(1.08)  LDD1689  [11]
 LDCM0491  CL96 HEK-293T C290(1.12)  LDD1694  [11]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C156(1.58)  LDD1702  [3]
 LDCM0625  F8 Ramos C290(1.94)  LDD2187  [12]
 LDCM0573  Fragment11 Ramos C290(2.80)  LDD2190  [12]
 LDCM0574  Fragment12 Ramos C290(0.78)  LDD2191  [12]
 LDCM0576  Fragment14 Ramos C290(1.04)  LDD2193  [12]
 LDCM0580  Fragment21 Ramos C290(1.48)  LDD2195  [12]
 LDCM0582  Fragment23 Ramos C290(0.98)  LDD2196  [12]
 LDCM0578  Fragment27 Ramos C290(0.75)  LDD2197  [12]
 LDCM0588  Fragment30 Ramos C290(2.38)  LDD2199  [12]
 LDCM0589  Fragment31 Ramos C290(0.93)  LDD2200  [12]
 LDCM0566  Fragment4 Ramos C290(0.99)  LDD2184  [12]
 LDCM0614  Fragment56 Ramos C290(1.21)  LDD2205  [12]
 LDCM0569  Fragment7 Ramos C290(1.22)  LDD2186  [12]
 LDCM0022  KB02 8305C C68(1.28)  LDD2248  [2]
 LDCM0023  KB03 Ramos C290(1.32)  LDD2183  [12]
 LDCM0024  KB05 MEL167 C68(2.55)  LDD3316  [2]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C156(0.97)  LDD2123  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cathepsin D (CTSD) Peptidase A1 family P07339
Iduronate 2-sulfatase (IDS) Sulfatase family P22304
Formylglycine-generating enzyme (SUMF1) Sulfatase-modifying factor family Q8NBK3
Inactive C-alpha-formylglycine-generating enzyme 2 (SUMF2) Sulfatase-modifying factor family Q8NBJ7
Transthyretin (TTR) Transthyretin family P02766
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Progranulin (GRN) Granulin family P28799
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578