Details of the Target

General Information of Target

Target ID LDTP02124
Target Name Keratin, type I cytoskeletal 18 (KRT18)
Gene Name KRT18
Gene ID 3875
Synonyms
CYK18; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGS
GGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKG
PQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVEN
DIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAP
KSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELR
RTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQA
QEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVS
ETNDTKVLRH
Target Bioclass
Other
Family
Intermediate filament family
Subcellular location
Nucleus matrix
Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Uniprot ID
P05783
Ensemble ID
ENST00000388835.4
HGNC ID
HGNC:6430

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 21 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-5
 Probe Info  		3.52  LDD0394  [1]
ILS-1
 Probe Info  		4.78; 6.61  LDD0415  [2]
AZ-9
 Probe Info  		4.87  LDD0393  [1]
FBPP2
 Probe Info  		2.60  LDD0324  [3]
YN-1
 Probe Info  		33.21  LDD0444  [4]
YN-4
 Probe Info  		100.00  LDD0445  [4]
ONAyne
 Probe Info  		K111(4.12); K167(2.90); K187(1.90); K215(3.78)  LDD0274  [5]
OPA-S-S-alkyne
 Probe Info  		K372(2.29); K187(3.47); K119(6.22); K118(6.22)  LDD3494  [6]
5E-2FA
 Probe Info  		H183(0.00); H344(0.00)  LDD2235  [7]
ATP probe
 Probe Info  		N.A.  LDD0199  [8]
m-APA
 Probe Info  		H183(0.00); H344(0.00); H128(0.00)  LDD2231  [7]
ATP probe
 Probe Info  		K131(0.00); K317(0.00); K417(0.00); K207(0.00)  LDD0035  [9]
NHS
 Probe Info  		N.A.  LDD0010  [10]
STPyne
 Probe Info  		K187(0.00); K426(0.00)  LDD0009  [10]
Acrolein
 Probe Info  		H183(0.00); H128(0.00); H217(0.00)  LDD0217  [11]
Crotonaldehyde
 Probe Info  		H183(0.00); H128(0.00); H217(0.00)  LDD0219  [11]
Methacrolein
 Probe Info  		H217(0.00); H183(0.00)  LDD0218  [11]
W1
 Probe Info  		N.A.  LDD0236  [12]
HHS-465
 Probe Info  		Y13(0.00); Y36(0.00); Y24(0.00)  LDD2240  [13]
HHS-475
 Probe Info  		Y13(0.85); Y168(0.66); Y24(0.81); Y256(0.78)  LDD2238  [14]
HHS-482
 Probe Info  		Y13(0.55); Y168(0.46); Y24(0.29); Y256(0.91)  LDD2239  [14]
PAL-AfBPP Probe
Click To Hide/Show 89 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DR-1
 Probe Info  		3.40  LDD0398  [15]
ILS-1 PP
 Probe Info  		2.34  LDD0417  [2]
C003
 Probe Info  		22.78  LDD1713  [16]
C004
 Probe Info  		5.66  LDD1714  [16]
C017
 Probe Info  		6.06  LDD1725  [16]
C022
 Probe Info  		7.52  LDD1728  [16]
C040
 Probe Info  		9.58  LDD1740  [16]
C041
 Probe Info  		11.31  LDD1741  [16]
C052
 Probe Info  		5.90  LDD1750  [16]
C055
 Probe Info  		19.03  LDD1752  [16]
C056
 Probe Info  		27.67  LDD1753  [16]
C063
 Probe Info  		16.80  LDD1760  [16]
C070
 Probe Info  		11.24  LDD1766  [16]
C087
 Probe Info  		33.36  LDD1779  [16]
C091
 Probe Info  		12.64  LDD1782  [16]
C092
 Probe Info  		21.11  LDD1783  [16]
C095
 Probe Info  		5.35  LDD1786  [16]
C102
 Probe Info  		5.90  LDD1790  [16]
C106
 Probe Info  		36.00  LDD1793  [16]
C107
 Probe Info  		5.98  LDD1794  [16]
C108
 Probe Info  		14.22  LDD1795  [16]
C112
 Probe Info  		41.07  LDD1799  [16]
C130
 Probe Info  		7.26  LDD1812  [16]
C138
 Probe Info  		5.24  LDD1820  [16]
C139
 Probe Info  		10.70  LDD1821  [16]
C145
 Probe Info  		11.31  LDD1827  [16]
C153
 Probe Info  		17.27  LDD1834  [16]
C158
 Probe Info  		16.56  LDD1838  [16]
C160
 Probe Info  		7.01  LDD1840  [16]
C161
 Probe Info  		11.31  LDD1841  [16]
C169
 Probe Info  		21.11  LDD1849  [16]
C187
 Probe Info  		16.80  LDD1865  [16]
C191
 Probe Info  		12.21  LDD1868  [16]
C201
 Probe Info  		39.40  LDD1877  [16]
C206
 Probe Info  		21.56  LDD1881  [16]
C210
 Probe Info  		99.73  LDD1884  [16]
C213
 Probe Info  		15.24  LDD1887  [16]
C216
 Probe Info  		5.82  LDD1890  [16]
C218
 Probe Info  		18.25  LDD1892  [16]
C220
 Probe Info  		16.00  LDD1894  [16]
C228
 Probe Info  		14.72  LDD1901  [16]
C232
 Probe Info  		44.02  LDD1905  [16]
C244
 Probe Info  		22.94  LDD1917  [16]
C246
 Probe Info  		14.72  LDD1919  [16]
C249
 Probe Info  		26.72  LDD1922  [16]
C269
 Probe Info  		9.38  LDD1939  [16]
C277
 Probe Info  		8.88  LDD1947  [16]
C284
 Probe Info  		47.50  LDD1954  [16]
C288
 Probe Info  		7.31  LDD1958  [16]
C289
 Probe Info  		38.85  LDD1959  [16]
C296
 Probe Info  		20.11  LDD1966  [16]
C305
 Probe Info  		14.32  LDD1974  [16]
C313
 Probe Info  		29.65  LDD1980  [16]
C314
 Probe Info  		16.68  LDD1981  [16]
C317
 Probe Info  		7.57  LDD1983  [16]
C320
 Probe Info  		5.35  LDD1986  [16]
C322
 Probe Info  		6.28  LDD1988  [16]
C326
 Probe Info  		8.40  LDD1990  [16]
C338
 Probe Info  		50.56  LDD2001  [16]
C339
 Probe Info  		5.24  LDD2002  [16]
C343
 Probe Info  		12.47  LDD2005  [16]
C346
 Probe Info  		18.51  LDD2007  [16]
C347
 Probe Info  		6.96  LDD2008  [16]
C348
 Probe Info  		15.14  LDD2009  [16]
C350
 Probe Info  		30.48  LDD2011  [16]
C353
 Probe Info  		6.11  LDD2014  [16]
C354
 Probe Info  		10.27  LDD2015  [16]
C355
 Probe Info  		44.32  LDD2016  [16]
C361
 Probe Info  		26.54  LDD2022  [16]
C362
 Probe Info  		55.33  LDD2023  [16]
C363
 Probe Info  		25.11  LDD2024  [16]
C364
 Probe Info  		23.43  LDD2025  [16]
C382
 Probe Info  		11.71  LDD2041  [16]
C383
 Probe Info  		17.27  LDD2042  [16]
C388
 Probe Info  		54.19  LDD2047  [16]
C389
 Probe Info  		5.39  LDD2048  [16]
C390
 Probe Info  		40.22  LDD2049  [16]
C391
 Probe Info  		59.30  LDD2050  [16]
C397
 Probe Info  		15.45  LDD2056  [16]
C403
 Probe Info  		23.43  LDD2061  [16]
C407
 Probe Info  		25.11  LDD2064  [16]
C413
 Probe Info  		17.75  LDD2069  [16]
C420
 Probe Info  		21.41  LDD2075  [16]
C429
 Probe Info  		13.18  LDD2084  [16]
C431
 Probe Info  		15.56  LDD2086  [16]
C433
 Probe Info  		9.13  LDD2088  [16]
STS-1
 Probe Info  		1.30  LDD0136  [17]
STS-2
 Probe Info  		1.80  LDD0138  [17]
DA-2
 Probe Info  		N.A.  LDD0072  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H183(0.00); H128(0.00); H217(0.00)  LDD0222  [11]
 LDCM0107  IAA HeLa H183(0.00); H128(0.00); H217(0.00)  LDD0221  [11]
 LDCM0109  NEM HeLa H183(0.00); H128(0.00); H217(0.00); H344(0.00)  LDD0223  [11]
 LDCM0112  W16 Hep-G2 N315(0.61); K317(0.67)  LDD0239  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
Tumor susceptibility gene 101 protein (TSG101) Ubiquitin-conjugating enzyme family Q99816
ADP-ribose glycohydrolase MACROD1 (MACROD1) . Q9BQ69
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Beclin-1 (BECN1) Beclin family Q14457
Huntingtin (HTT) Huntingtin family P42858
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3) . O75022
Other
Click To Hide/Show 17 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
HAUS augmin-like complex subunit 1 (HAUS1) HAUS1 family Q96CS2
Keratin, type II cuticular Hb1 (KRT81) Intermediate filament family Q14533
Keratin, type II cytoskeletal 6A (KRT6A) Intermediate filament family P02538
Keratin, type II cytoskeletal 6C (KRT6C) Intermediate filament family P48668
Keratin, type II cytoskeletal 71 (KRT71) Intermediate filament family Q3SY84
Keratin, type II cytoskeletal 79 (KRT79) Intermediate filament family Q5XKE5
Keratin, type II cytoskeletal 8 (KRT8) Intermediate filament family P05787
Neurofilament light polypeptide (NEFL) Intermediate filament family P07196
Synaptonemal complex central element protein 1 (SYCE1) SYCE family Q8N0S2
Tektin-4 (TEKT4) Tektin family Q8WW24
AFG2-interacting ribosome maturation factor (AIRIM) . Q9NX04
DnaJ homolog subfamily B member 6 (DNAJB6) . O75190
EF-hand domain-containing protein 1 (EFHC1) . Q5JVL4
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
NF-kappa-B essential modulator (IKBKG) . Q9Y6K9
Tumor necrosis factor receptor type 1-associated DEATH domain protein (TRADD) . Q15628

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 A Chemical Proteomic Analysis of Illudin-Interacting Proteins. Chemistry. 2019 Sep 25;25(54):12644-12651. doi: 10.1002/chem.201902919. Epub 2019 Sep 3.
Mass spectrometry data entry: PXD014175
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
13 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
15 Quantitative Proteomics Reveals Cellular Off-Targets of a DDR1 Inhibitor. ACS Med Chem Lett. 2020 Feb 5;11(4):535-540. doi: 10.1021/acsmedchemlett.9b00658. eCollection 2020 Apr 9.
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
18 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.