Details of the Target

General Information of Target

Target ID LDTP06352
Target Name Reticulocalbin-1 (RCN1)
Gene Name RCN1
Gene ID 5954
Synonyms
RCN; Reticulocalbin-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARGGRGRRLGLALGLLLALVLAPRVLRAKPTVRKERVVRPDSELGERPPEDNQSFQYDH
EAFLGKEDSKTFDQLTPDESKERLGKIVDRIDNDGDGFVTTEELKTWIKRVQKRYIFDNV
AKVWKDYDRDKDDKISWEEYKQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKAADL
NGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMFSHEENGP
EPDWVLSEREQFNEFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEKL
TKEEILENWNMFVGSQATNYGEDLTKNHDEL
Target Bioclass
Other
Family
CREC family
Subcellular location
Endoplasmic reticulum lumen
Function May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Uniprot ID
Q15293
Ensemble ID
ENST00000054950.4
HGNC ID
HGNC:9934

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ONS76 SNV: p.M1? .
SW1116 SNV: p.A193S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		12.73  LDD0318  [1]
TH211
 Probe Info  		Y115(12.48); Y290(11.65)  LDD0257  [2]
TH216
 Probe Info  		Y127(20.00); Y290(20.00); Y115(19.71)  LDD0259  [2]
STPyne
 Probe Info  		K105(5.11); K122(2.19); K165(7.57); K176(6.89)  LDD0277  [3]
ONAyne
 Probe Info  		N.A.  LDD0273  [3]
HHS-482
 Probe Info  		Y115(0.97)  LDD0285  [4]
HHS-475
 Probe Info  		Y278(0.78); Y290(0.95); Y320(9.33)  LDD0264  [5]
HHS-465
 Probe Info  		Y115(8.13)  LDD2237  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
ATP probe
 Probe Info  		K122(0.00); K125(0.00); K105(0.00); K109(0.00)  LDD0199  [8]
m-APA
 Probe Info  		H271(0.00); H60(0.00); H280(0.00)  LDD2231  [7]
SF
 Probe Info  		N.A.  LDD0028  [9]
Ox-W18
 Probe Info  		W309(0.00); W272(0.00)  LDD2175  [10]
1c-yne
 Probe Info  		K176(0.00); K302(0.00)  LDD0228  [11]
1d-yne
 Probe Info  		N.A.  LDD0357  [11]
Acrolein
 Probe Info  		H271(0.00); H287(0.00); H196(0.00); H280(0.00)  LDD0217  [12]
Crotonaldehyde
 Probe Info  		H271(0.00); H280(0.00)  LDD0219  [12]
PAL-AfBPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C017
 Probe Info  		5.78  LDD1725  [13]
C052
 Probe Info  		5.39  LDD1750  [13]
C087
 Probe Info  		8.11  LDD1779  [13]
C131
 Probe Info  		5.46  LDD1813  [13]
C165
 Probe Info  		13.27  LDD1845  [13]
C219
 Probe Info  		6.82  LDD1893  [13]
C230
 Probe Info  		10.13  LDD1903  [13]
C233
 Probe Info  		18.25  LDD1906  [13]
C338
 Probe Info  		10.78  LDD2001  [13]
FFF probe11
 Probe Info  		12.52  LDD0471  [14]
FFF probe12
 Probe Info  		12.88  LDD0473  [14]
FFF probe13
 Probe Info  		12.62  LDD0475  [14]
FFF probe14
 Probe Info  		20.00  LDD0477  [14]
FFF probe15
 Probe Info  		20.00  LDD0478  [14]
FFF probe2
 Probe Info  		14.18  LDD0463  [14]
FFF probe3
 Probe Info  		12.95  LDD0464  [14]
FFF probe4
 Probe Info  		20.00  LDD0466  [14]
FFF probe6
 Probe Info  		14.68  LDD0482  [14]
FFF probe7
 Probe Info  		20.00  LDD0483  [14]
FFF probe9
 Probe Info  		20.00  LDD0470  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H287(0.00); H271(0.00); H196(0.00); H202(0.00)  LDD0222  [12]
 LDCM0116  HHS-0101 DM93 Y278(0.78); Y290(0.95); Y320(9.33)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y278(0.53); Y290(1.73)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y278(0.67); Y290(1.64)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y278(0.95); Y290(1.75)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y290(1.07); Y278(1.12); Y320(1.77)  LDD0268  [5]
 LDCM0107  IAA HeLa H271(0.00); H287(0.00); H280(0.00); H196(0.00)  LDD0221  [12]
 LDCM0123  JWB131 DM93 Y115(0.97)  LDD0285  [4]
 LDCM0124  JWB142 DM93 Y115(1.39)  LDD0286  [4]
 LDCM0125  JWB146 DM93 Y115(1.25)  LDD0287  [4]
 LDCM0126  JWB150 DM93 Y115(2.06)  LDD0288  [4]
 LDCM0127  JWB152 DM93 Y115(1.66)  LDD0289  [4]
 LDCM0128  JWB198 DM93 Y115(0.31)  LDD0290  [4]
 LDCM0129  JWB202 DM93 Y115(1.28)  LDD0291  [4]
 LDCM0130  JWB211 DM93 Y115(7.93)  LDD0292  [4]
 LDCM0109  NEM HeLa H271(0.00); H280(0.00); H287(0.00); H196(0.00)  LDD0223  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATPase GET3 (GET3) ArsA ATPase family O43681
tRNA modification GTPase GTPBP3, mitochondrial (GTPBP3) TrmE GTPase family Q969Y2
DDB1- and CUL4-associated factor 11 (DCAF11) . Q8TEB1
Sulfite oxidase, mitochondrial (SUOX) . P51687
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901
Other
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) ATP12 family Q8N5M1
Ataxin-1 (ATXN1) ATXN1 family P54253
Centrin-3 (CETN3) Centrin family O15182
Protein FAM110A (FAM110A) FAM110 family Q9BQ89
Growth arrest and DNA damage-inducible protein GADD45 gamma (GADD45G) GADD45 family O95257
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) SGT family O43765
Small glutamine-rich tetratricopeptide repeat-containing protein beta (SGTB) SGT family Q96EQ0
EF-hand domain-containing protein 1 (EFHC1) . Q5JVL4
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
LIM domain transcription factor LMO4 (LMO4) . P61968
Rhombotin-1 (LMO1) . P25800
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
10 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
11 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.