Details of the Target

General Information of Target

Target ID LDTP06633
Target Name Reticulon-1 (RTN1)
Gene Name RTN1
Gene ID 6252
Synonyms
NSP; Reticulon-1; Neuroendocrine-specific protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPGDPQDELLPLAGPGSQWLRHRGEGENEAVTPKGATPAPQAGEPSPGLGARAREAAS
REAGSGPARQSPVAMETASTGVAGVSSAMDHTFSTTSKDGEGSCYTSLISDICYPPQEDS
TYFTGILQKENGHVTISESPEELGTPGPSLPDVPGIESRGLFSSDSGIEMTPAESTEVNK
ILADPLDQMKAEAYKYIDITRPEEVKHQEQHHPELEDKDLDFKNKDTDISIKPEGVREPD
KPAPVEGKIIKDHLLEESTFAPYIDDLSEEQRRAPQITTPVKITLTEIEPSVETTTQEKT
PEKQDICLKPSPDTVPTVTVSEPEDDSPGSITPPSSGTEPSAAESQGKGSISEDELITAI
KEAKGLSYETAENPRPVGQLADRPEVKARSGPPTIPSPLDHEASSAESGDSEIELVSEDP
MAAEDALPSGYVSFGHVGGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEES
PKREQDSPPMKPSALDAIREETGVRAEERAPSRRGLAEPGSFLDYPSTEPQPGPELPPGD
GALEPETPMLPRKPEEDSSSNQSPAATKGPGPLGPGAPPPLLFLNKQKAIDLLYWRDIKQ
TGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAY
LELEITLSQEQIQKYTDCLQFYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFN
GLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKIQAKIPGAKRHAE
Target Bioclass
Transporter and channel
Subcellular location
Endoplasmic reticulum membrane
Function Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity.
Uniprot ID
Q16799
Ensemble ID
ENST00000267484.10
HGNC ID
HGNC:10467

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A673 SNV: p.E362Q .
DU145 SNV: p.G66S; p.V761E .
HCT116 SNV: p.A510V .
HEC1 SNV: p.H253Y .
IGR1 SNV: p.L285P .
IPC298 SNV: p.G391R .
MFE296 Deletion: p.E27RfsTer7 .
NCIH358 SNV: p.P148H .
OVK18 Deletion: p.L399WfsTer34 .
PC9 SNV: p.P538H .
SF295 SNV: p.T635A .
SHP77 Substitution: p.P280S DBIA    Probe Info 
SKOV3 SNV: p.G37E .
TE4 SNV: p.R499L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
FBPP2
 Probe Info  		3.25  LDD0318  [2]
DA-P2
 Probe Info  		52.30  LDD0348  [3]
HRP
 Probe Info  		35.42  LDD0347  [3]
SAA-alkyne
 Probe Info  		1.10  LDD0252  [4]
FBP2
 Probe Info  		4.52  LDD0317  [2]
DBIA
 Probe Info  		C678(4.56)  LDD3344  [5]
Sulforaphane-probe2
 Probe Info  		1.58  LDD0160  [6]
DA-P3
 Probe Info  		6.84  LDD0180  [3]
HPAP
 Probe Info  		5.14  LDD0064  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 30 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
PARPYnD
 Probe Info  		1.37  LDD0374  [9]
C040
 Probe Info  		10.41  LDD1740  [10]
C049
 Probe Info  		4.92  LDD1747  [10]
C091
 Probe Info  		17.51  LDD1782  [10]
C092
 Probe Info  		25.81  LDD1783  [10]
C094
 Probe Info  		37.53  LDD1785  [10]
C228
 Probe Info  		18.13  LDD1901  [10]
C251
 Probe Info  		57.28  LDD1924  [10]
C252
 Probe Info  		31.34  LDD1925  [10]
C264
 Probe Info  		69.55  LDD1935  [10]
C265
 Probe Info  		38.32  LDD1936  [10]
C266
 Probe Info  		7.84  LDD1937  [10]
C270
 Probe Info  		12.82  LDD1940  [10]
C285
 Probe Info  		30.91  LDD1955  [10]
C287
 Probe Info  		10.06  LDD1957  [10]
C289
 Probe Info  		55.33  LDD1959  [10]
C293
 Probe Info  		20.97  LDD1963  [10]
C322
 Probe Info  		9.99  LDD1988  [10]
C326
 Probe Info  		7.36  LDD1990  [10]
C343
 Probe Info  		38.32  LDD2005  [10]
C348
 Probe Info  		27.10  LDD2009  [10]
C349
 Probe Info  		72.50  LDD2010  [10]
C350
 Probe Info  		99.73  LDD2011  [10]
C355
 Probe Info  		56.89  LDD2016  [10]
C356
 Probe Info  		28.64  LDD2017  [10]
C357
 Probe Info  		11.08  LDD2018  [10]
C362
 Probe Info  		39.95  LDD2023  [10]
C364
 Probe Info  		14.83  LDD2025  [10]
C373
 Probe Info  		16.45  LDD2033  [10]
C407
 Probe Info  		11.39  LDD2064  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 12.37  LDD0403  [1]
 LDCM0087  Capsaicin HEK-293T 23.11  LDD0185  [3]
 LDCM0028  Dobutamine HEK-293T 6.84  LDD0180  [3]
 LDCM0031  Epigallocatechin gallate HEK-293T 10.17  LDD0183  [3]
 LDCM0022  KB02 BRX211 C678(5.64)  LDD2268  [5]
 LDCM0023  KB03 BRX211 C678(12.42)  LDD2685  [5]
 LDCM0024  KB05 NCI-H146 C678(4.56)  LDD3344  [5]
 LDCM0032  Oleacein HEK-293T 4.67  LDD0184  [3]
 LDCM0014  Panhematin hPBMC 5.14  LDD0064  [7]
 LDCM0029  Quercetin HEK-293T 21.12  LDD0181  [3]
 LDCM0003  Sulforaphane MDA-MB-231 1.58  LDD0160  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Syntenin-1 (SDCBP) . O00560
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mesencephalic astrocyte-derived neurotrophic factor (MANF) ARMET family P55145

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
4 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Chem Commun (Camb). 2017 May 4;53(37):5182-5185. doi: 10.1039/c6cc08797c.
Mass spectrometry data entry: PXD006279
7 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Structure-Guided Design and In-Cell Target Profiling of a Cell-Active Target Engagement Probe for PARP Inhibitors. ACS Chem Biol. 2020 Feb 21;15(2):325-333. doi: 10.1021/acschembio.9b00963. Epub 2020 Feb 10.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587