Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name Sulforaphane-probe2  Probe Info 
Probe concentration 5 uM
Criteria Quantification: Probe vs (Probe+Competitor)
Quantitative Method
SILAC
Competitor Name Sulforaphane  Competitor Info 
Competitor Concentration 100 uM
In Vivo Experiment Model Model Type Living cell
Derived Tissue Breast
Disease Model Breast adenocarcinoma [ICD-11:2C60]
Model Name Human breast adenocarcinoma cells (MDA-MB-231)

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 50 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12539  DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C) 5.1
 LDTP05733  Serine/threonine-protein kinase 4 (STK4) 3.7
 LDTP02074  Aldehyde dehydrogenase, mitochondrial (ALDH2) 3.6
 LDTP11107  Serine/threonine-protein phosphatase CPPED1 (CPPED1) 3.1
 LDTP14240  Serine/threonine-protein kinase 24 (STK24) 3.0
 LDTP04232  4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) 2.8
 LDTP02713  Macrophage migration inhibitory factor (MIF) 2.6
 LDTP00544  Sphingolipid delta(4)-desaturase DES1 (DEGS1) 2.6
 LDTP00267  DNA-directed RNA polymerase, mitochondrial (POLRMT) 2.6
 LDTP08352  Histone-arginine methyltransferase CARM1 (CARM1) 2.5
 LDTP03496  Protein PML (PML) 2.5
 LDTP07054  Exosome complex component MTR3 (EXOSC6) 2.4
 LDTP07317  Tensin-3 (TNS3) 2.4
 LDTP04471  Ribosomal protein S6 kinase alpha-3 (RPS6KA3) 2.3
 LDTP05046  Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform (PPP2R2A) 2.3
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 2.3
 LDTP12835  Large ribosomal subunit protein mL39 (MRPL39) 2.2
 LDTP03859  V-type proton ATPase catalytic subunit A (ATP6V1A) 2.2
 LDTP13147  Cathepsin Z (CTSZ) 2.2
 LDTP06190  Ubiquitin carboxyl-terminal hydrolase 10 (USP10) 2.1
 LDTP00225  26S proteasome non-ATPase regulatory subunit 9 (PSMD9) 2.1
 LDTP03540  Heme oxygenase 2 (HMOX2) 2.0
 LDTP01238  NADH dehydrogenase iron-sulfur protein 3, mitochondrial (NDUFS3) 2.0
 LDTP10935  Kinesin-like protein KIF2C (KIF2C) 2.0
 LDTP04581  Holocytochrome c-type synthase (HCCS) 2.0
 LDTP00479  Histone acetyltransferase type B catalytic subunit (HAT1) 2.0
 LDTP05242  Cyclin-dependent kinase 3 (CDK3) 2.0
 LDTP04163  Prolyl endopeptidase (PREP) 1.9
 LDTP02984  1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1) 1.9
 LDTP14535  6-phosphogluconolactonase (PGLS) 1.9
 LDTP05881  Rho-associated protein kinase 1 (ROCK1) 1.9
 LDTP05475  Tyrosine-protein phosphatase non-receptor type 11 (PTPN11) 1.8
 LDTP06384  Ribosomal protein S6 kinase alpha-1 (RPS6KA1) 1.8
 LDTP19669  Threonine synthase-like 1 (THNSL1) 1.8
 LDTP03110  Cytoplasmic aconitate hydratase (ACO1) 1.7
 LDTP02497  Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT) 1.7
 LDTP04263  Cysteine--tRNA ligase, cytoplasmic (CARS1) 1.7
 LDTP15993  Aminopeptidase B (RNPEP) 1.7
 LDTP03259  Ribonucleoside-diphosphate reductase large subunit (RRM1) 1.7
 LDTP07651  Monofunctional C1-tetrahydrofolate synthase, mitochondrial (MTHFD1L) 1.6
 LDTP06883  Fibronectin type III domain-containing protein 3B (FNDC3B) 1.6
 LDTP06839  NAD kinase 2, mitochondrial (NADK2) 1.6
 LDTP01785  Epidermal growth factor receptor (EGFR) 1.6
 LDTP02935  Tyrosine-protein phosphatase non-receptor type 1 (PTPN1) 1.5
 LDTP02770  Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) 1.5
 LDTP03900  ADP-ribosylation factor-like protein 1 (ARL1) 1.4
 LDTP00601  UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) 1.4
 LDTP13449  Cleavage and polyadenylation specificity factor subunit 3 (CPSF3) 1.4
 LDTP04358  Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) 1.3
 LDTP08617  Patatin-like phospholipase domain-containing protein 6 (PNPLA6) 0.9
Transporter and channel
Click to Show/Hide to Show 10 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP00970  Gasdermin-E (GSDME) 3.1
 LDTP04801  Gasdermin-D (GSDMD) 2.9
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 2.3
 LDTP08029  Nucleoporin p54 (NUP54) 2.2
 LDTP13243  Translocation protein SEC63 homolog (SEC63) 2.0
 LDTP00958  TBC1 domain family member 4 (TBC1D4) 1.8
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 1.8
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 1.6
 LDTP06633  Reticulon-1 (RTN1) 1.6
 LDTP11161  Extended synaptotagmin-1 (ESYT1) 1.4
Transcription factor
Click to Show/Hide to Show 6 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP05254  Nuclear factor NF-kappa-B p100 subunit (NFKB2) 2.2
 LDTP02016  Glucocorticoid receptor (NR3C1) 2.0
 LDTP03903  Signal transducer and activator of transcription 3 (STAT3) 1.9
 LDTP05417  Transcription factor p65 (RELA) 1.8
 LDTP03280  COUP transcription factor 2 (NR2F2) 1.7
 LDTP12792  CDKN2A-interacting protein (CDKN2AIP) 1.5
Immunoglobulin
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP09680  Palladin (PALLD) 1.1
Other
Click to Show/Hide to Show 54 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP06051  Kelch-like ECH-associated protein 1 (KEAP1) 5.9
 LDTP18309  Protein HGH1 homolog (HGH1) 2.7
 LDTP04396  RNA-binding protein FXR2 (FXR2) 2.7
 LDTP13935  Protein SGT1 homolog (SUGT1) 2.5
 LDTP04078  Proliferation marker protein Ki-67 (MKI67) 2.5
 LDTP04395  RNA-binding protein FXR1 (FXR1) 2.5
 LDTP08273  Leucine zipper protein 1 (LUZP1) 2.4
 LDTP11197  Mini-chromosome maintenance complex-binding protein (MCMBP) 2.3
 LDTP00580  Zinc finger protein 185 (ZNF185) 2.3
 LDTP00239  DNA fragmentation factor subunit alpha (DFFA) 2.3
 LDTP12443  Anillin (ANLN) 2.2
 LDTP06281  Condensin complex subunit 1 (NCAPD2) 2.2
 LDTP16155  Opioid growth factor receptor (OGFR) 2.2
 LDTP13583  Targeting protein for Xklp2 (TPX2) 2.2
 LDTP06880  Rho GTPase-activating protein 29 (ARHGAP29) 2.1
 LDTP18116  PHD finger protein 3 (PHF3) 2.1
 LDTP06378  Disks large-associated protein 5 (DLGAP5) 2.1
 LDTP01021  Perilipin-3 (PLIN3) 2.1
 LDTP06268  Condensin complex subunit 2 (NCAPH) 2.0
 LDTP06193  Raftlin (RFTN1) 2.0
 LDTP09196  Biorientation of chromosomes in cell division protein 1-like 1 (BOD1L1) 2.0
 LDTP04434  Host cell factor 1 (HCFC1) 2.0
 LDTP16102  SPATS2-like protein (SPATS2L) 2.0
 LDTP14037  Microtubule cross-linking factor 1 (MTCL1) 2.0
 LDTP05557  Golgin subfamily A member 2 (GOLGA2) 2.0
 LDTP06310  Early endosome antigen 1 (EEA1) 2.0
 LDTP10834  RUN and FYVE domain-containing protein 1 (RUFY1) 2.0
 LDTP00512  Protein transport protein Sec16A (SEC16A) 1.9
 LDTP08735  Spartin (SPART) 1.9
 LDTP07755  Dynamin-binding protein (DNMBP) 1.9
 LDTP04743  Eukaryotic translation initiation factor 6 (EIF6) 1.9
 LDTP03967  Lamina-associated polypeptide 2, isoform alpha (TMPO) 1.9
 LDTP01522  Reticulon-3 (RTN3) 1.9
 LDTP10843  MMS19 nucleotide excision repair protein homolog (MMS19) 1.9
 LDTP09383  Protein CIP2A (CIP2A) 1.9
 LDTP04226  Paxillin (PXN) 1.8
 LDTP12883  Constitutive coactivator of PPAR-gamma-like protein 1 (FAM120A) 1.8
 LDTP11597  182 kDa tankyrase-1-binding protein (TNKS1BP1) 1.8
 LDTP13519  Proteasome activator complex subunit 2 (PSME2) 1.8
 LDTP16107  TBC1 domain family member 13 (TBC1D13) 1.8
 LDTP13591  A-kinase anchor protein 8-like (AKAP8L) 1.8
 LDTP01210  Transforming acidic coiled-coil-containing protein 1 (TACC1) 1.8
 LDTP03079  Calpastatin (CAST) 1.7
 LDTP03557  Leukocyte elastase inhibitor (SERPINB1) 1.7
 LDTP00202  AH receptor-interacting protein (AIP) 1.7
 LDTP05049  Dynein light chain 1, cytoplasmic (DYNLL1) 1.7
 LDTP09837  A-kinase anchor protein 1, mitochondrial (AKAP1) 1.6
 LDTP04115  Transcriptional coactivator YAP1 (YAP1) 1.6
 LDTP05533  Kinesin light chain 1 (KLC1) 1.6
 LDTP08535  MAP7 domain-containing protein 3 (MAP7D3) 1.6
 LDTP04127  F-actin-capping protein subunit beta (CAPZB) 1.5
 LDTP05770  Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 (AIMP2) 1.4
 LDTP14250  Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) 1.3
 LDTP11930  Oxysterol-binding protein-related protein 3 (OSBPL3) 1.1

References

1 Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Chem Commun (Camb). 2017 May 4;53(37):5182-5185. doi: 10.1039/c6cc08797c.
Mass spectrometry data entry: PXD006279