Details of the Target

General Information of Target

Target ID LDTP05533
Target Name Kinesin light chain 1 (KLC1)
Gene Name KLC1
Gene ID 3831
Synonyms
KLC; KNS2; Kinesin light chain 1; KLC 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDD
ESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWL
RDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF
PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQAL
EDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATL
NNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEY
YYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVD
DENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKF
EAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGE
EVSMSVEWNGGVSGRASFCGKRQQQQWPGRRHR
Target Bioclass
Other
Family
Kinesin light chain family
Subcellular location
Cell projection, growth cone
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Uniprot ID
Q07866
Ensemble ID
ENST00000246489.11
HGNC ID
HGNC:6387

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 30 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
CY-1
 Probe Info  		100.00  LDD0243  [2]
TH216
 Probe Info  		Y223(10.61)  LDD0259  [3]
ONAyne
 Probe Info  		K503(0.43)  LDD0274  [4]
STPyne
 Probe Info  		K27(10.00); K272(6.35); K372(0.92); K398(3.85)  LDD0277  [4]
Probe 1
 Probe Info  		Y355(3.71); Y360(48.52); Y369(12.64)  LDD3495  [5]
DBIA
 Probe Info  		C621(1.35)  LDD3310  [6]
BTD
 Probe Info  		C320(1.10); C559(1.28); C390(0.75); C349(1.23)  LDD2099  [7]
Sulforaphane-probe2
 Probe Info  		1.63  LDD0160  [8]
AHL-Pu-1
 Probe Info  		C114(2.60)  LDD0169  [9]
HHS-482
 Probe Info  		Y223(1.44); Y307(0.65)  LDD0285  [10]
HHS-475
 Probe Info  		Y265(0.68); Y449(0.91); Y223(0.97); Y307(1.08)  LDD0264  [11]
HHS-465
 Probe Info  		Y223(10.00); Y307(7.29); Y449(10.00)  LDD2237  [12]
5E-2FA
 Probe Info  		H147(0.00); H217(0.00)  LDD2235  [13]
ATP probe
 Probe Info  		K309(0.00); K393(0.00); K272(0.00); K457(0.00)  LDD0199  [14]
4-Iodoacetamidophenylacetylene
 Probe Info  		C114(0.00); C349(0.00); C390(0.00); C236(0.00)  LDD0038  [15]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [16]
Lodoacetamide azide
 Probe Info  		C320(0.00); C114(0.00); C390(0.00); C349(0.00)  LDD0037  [15]
NAIA_4
 Probe Info  		C114(0.00); C456(0.00); C320(0.00); C390(0.00)  LDD2226  [17]
TFBX
 Probe Info  		C456(0.00); C236(0.00)  LDD0027  [18]
Compound 10
 Probe Info  		N.A.  LDD2216  [19]
Compound 11
 Probe Info  		N.A.  LDD2213  [19]
IPM
 Probe Info  		C559(0.00); C114(0.00)  LDD0005  [20]
NHS
 Probe Info  		N.A.  LDD0010  [20]
PF-06672131
 Probe Info  		N.A.  LDD0017  [21]
Phosphinate-6
 Probe Info  		C114(0.00); C559(0.00); C320(0.00); C456(0.00)  LDD0018  [22]
1c-yne
 Probe Info  		K479(0.00); K309(0.00); K74(0.00)  LDD0228  [23]
Acrolein
 Probe Info  		N.A.  LDD0217  [24]
W1
 Probe Info  		N218(0.00); H217(0.00)  LDD0236  [25]
NAIA_5
 Probe Info  		C456(0.00); C55(0.00); C320(0.00); C390(0.00)  LDD2223  [17]
PAL-AfBPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C158
 Probe Info  		9.58  LDD1838  [26]
C187
 Probe Info  		16.68  LDD1865  [26]
C191
 Probe Info  		9.51  LDD1868  [26]
C275
 Probe Info  		7.01  LDD1945  [26]
FFF probe11
 Probe Info  		6.67  LDD0472  [27]
FFF probe13
 Probe Info  		20.00  LDD0475  [27]
FFF probe14
 Probe Info  		16.49  LDD0477  [27]
FFF probe2
 Probe Info  		20.00  LDD0463  [27]
FFF probe3
 Probe Info  		9.11  LDD0464  [27]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C320(1.48); C236(1.30); C559(1.22); C114(1.14)  LDD2117  [7]
 LDCM0025  4SU-RNA DM93 C436(2.26)  LDD0170  [9]
 LDCM0026  4SU-RNA+native RNA HEK-293T C114(2.60)  LDD0169  [9]
 LDCM0156  Aniline NCI-H1299 11.53  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C114(20.00); C236(2.90); C114(1.17); C390(1.06)  LDD2227  [17]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C114(0.92); C559(0.81)  LDD1702  [7]
 LDCM0625  F8 Ramos C236(1.64)  LDD2187  [28]
 LDCM0572  Fragment10 Ramos C236(1.07)  LDD2189  [28]
 LDCM0573  Fragment11 Ramos C236(3.34)  LDD2190  [28]
 LDCM0574  Fragment12 Ramos C236(0.67)  LDD2191  [28]
 LDCM0575  Fragment13 Ramos C236(0.96)  LDD2192  [28]
 LDCM0576  Fragment14 Ramos C236(0.99)  LDD2193  [28]
 LDCM0579  Fragment20 Ramos C236(0.44)  LDD2194  [28]
 LDCM0580  Fragment21 Ramos C236(1.01)  LDD2195  [28]
 LDCM0582  Fragment23 Ramos C236(0.88)  LDD2196  [28]
 LDCM0578  Fragment27 Ramos C236(0.94)  LDD2197  [28]
 LDCM0586  Fragment28 Ramos C236(0.48)  LDD2198  [28]
 LDCM0588  Fragment30 Ramos C236(1.01)  LDD2199  [28]
 LDCM0589  Fragment31 Ramos C236(1.10)  LDD2200  [28]
 LDCM0590  Fragment32 Ramos C236(0.96)  LDD2201  [28]
 LDCM0468  Fragment33 Ramos C236(0.79)  LDD2202  [28]
 LDCM0596  Fragment38 Ramos C236(0.63)  LDD2203  [28]
 LDCM0566  Fragment4 Ramos C236(0.60)  LDD2184  [28]
 LDCM0610  Fragment52 Ramos C236(0.86)  LDD2204  [28]
 LDCM0614  Fragment56 Ramos C236(1.45)  LDD2205  [28]
 LDCM0569  Fragment7 Ramos C236(0.58)  LDD2186  [28]
 LDCM0571  Fragment9 Ramos C236(1.85)  LDD2188  [28]
 LDCM0149  GA MCF-7 C55(2.60)  LDD0379  [29]
 LDCM0116  HHS-0101 DM93 Y265(0.68); Y449(0.91); Y223(0.97); Y307(1.08)  LDD0264  [11]
 LDCM0117  HHS-0201 DM93 Y223(0.80); Y265(0.82); Y307(0.82); Y313(1.49)  LDD0265  [11]
 LDCM0118  HHS-0301 DM93 Y313(0.06); Y265(0.60); Y449(0.71); Y223(0.71)  LDD0266  [11]
 LDCM0119  HHS-0401 DM93 Y265(0.72); Y223(0.74); Y307(0.91); Y449(0.92)  LDD0267  [11]
 LDCM0120  HHS-0701 DM93 Y307(0.85); Y223(0.92); Y265(0.99); Y449(1.03)  LDD0268  [11]
 LDCM0107  IAA HeLa N.A.  LDD0221  [24]
 LDCM0123  JWB131 DM93 Y223(1.44); Y307(0.65)  LDD0285  [10]
 LDCM0124  JWB142 DM93 Y223(0.28); Y307(0.70)  LDD0286  [10]
 LDCM0125  JWB146 DM93 Y223(2.28); Y307(0.80)  LDD0287  [10]
 LDCM0126  JWB150 DM93 Y223(3.79); Y307(2.18)  LDD0288  [10]
 LDCM0127  JWB152 DM93 Y223(2.15); Y307(1.29)  LDD0289  [10]
 LDCM0128  JWB198 DM93 Y223(1.52); Y307(0.39)  LDD0290  [10]
 LDCM0129  JWB202 DM93 Y223(0.47); Y307(0.50)  LDD0291  [10]
 LDCM0130  JWB211 DM93 Y223(1.04); Y307(0.78)  LDD0292  [10]
 LDCM0022  KB02 T cell-activated C559(5.18)  LDD1704  [30]
 LDCM0023  KB03 Jurkat C390(4.09); C456(7.91)  LDD0315  [16]
 LDCM0024  KB05 COLO792 C621(1.35)  LDD3310  [6]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C236(1.02)  LDD2102  [7]
 LDCM0109  NEM HeLa N.A.  LDD0223  [24]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C320(1.10); C559(1.28); C390(0.75); C349(1.23)  LDD2099  [7]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C320(0.81)  LDD2105  [7]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C320(0.97); C559(1.09)  LDD2107  [7]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C349(0.56)  LDD2109  [7]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C559(0.31)  LDD2114  [7]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C320(2.57)  LDD2119  [7]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C320(0.84)  LDD2123  [7]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C236(0.97); C114(1.44); C349(0.89)  LDD2125  [7]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C320(1.39); C236(1.32); C559(0.78)  LDD2127  [7]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C390(1.13)  LDD2136  [7]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C236(1.14); C559(1.19); C390(1.20)  LDD2137  [7]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C320(1.20); C236(0.98)  LDD2140  [7]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C390(1.42)  LDD2147  [7]
 LDCM0627  NUDT7-COV-1 HEK-293T C456(0.67)  LDD2206  [31]
 LDCM0628  OTUB2-COV-1 HEK-293T C456(0.99)  LDD2207  [31]
 LDCM0003  Sulforaphane MDA-MB-231 1.63  LDD0160  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) . Q12982
Caytaxin (ATCAY) . Q86WG3
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
FHF complex subunit HOOK-interacting protein 1A (FHIP1A) FHIP family Q05DH4
Keratin, type I cuticular Ha1 (KRT31) Intermediate filament family Q15323
C-Jun-amino-terminal kinase-interacting protein 3 (MAPK8IP3) JIP scaffold family Q9UPT6
Outer dynein arm-docking complex subunit 1 (ODAD1) ODA1/DCC2 family Q96M63
Transforming acidic coiled-coil-containing protein 3 (TACC3) TACC family Q9Y6A5

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane. Chem Commun (Camb). 2017 May 4;53(37):5182-5185. doi: 10.1039/c6cc08797c.
Mass spectrometry data entry: PXD006279
9 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
10 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
11 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
12 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
13 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
14 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
15 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
16 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
17 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
18 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
19 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
20 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
21 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
22 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
23 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
24 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
25 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
26 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
27 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
28 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
29 Combined Omics Approach Identifies Gambogic Acid and Related Xanthones as Covalent Inhibitors of the Serine Palmitoyltransferase Complex. Cell Chem Biol. 2020 May 21;27(5):586-597.e12. doi: 10.1016/j.chembiol.2020.03.008. Epub 2020 Apr 23.
30 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
31 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.