General Information of Target

Target ID LDTP06365
Target Name Transmembrane emp24 domain-containing protein 2 (TMED2)
Gene Name TMED2
Gene ID 10959
Synonyms
RNP24; Transmembrane emp24 domain-containing protein 2; Membrane protein p24A; p24; p24 family protein beta-1; p24beta1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVTLAELLVLLAALLATVSGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVE
ITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQD
METEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVL
VAMTLGQIYYLKRFFEVRRVV
Target Bioclass
Transporter and channel
Family
EMP24/GP25L family
Subcellular location
Cytoplasmic vesicle membrane
Function
Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.
Uniprot ID
Q15363
Ensemble ID
ENST00000262225.8
HGNC ID
HGNC:16996

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info 
K71(0.84)  LDD0274  [1]
BTD
 Probe Info 
C93(2.14)  LDD1699  [2]
DBIA
 Probe Info 
C93(1.37)  LDD3338  [3]
HPAP
 Probe Info 
3.16  LDD0063  [4]
IPM
 Probe Info 
C93(4.37)  LDD1701  [2]
5E-2FA
 Probe Info 
N.A.  LDD2235  [5]
m-APA
 Probe Info 
N.A.  LDD2231  [5]
Ox-W18
 Probe Info 
N.A.  LDD2175  [6]
1c-yne
 Probe Info 
N.A.  LDD0228  [7]
Acrolein
 Probe Info 
H146(0.00); H126(0.00)  LDD0217  [8]
Crotonaldehyde
 Probe Info 
H146(0.00); C93(0.00)  LDD0219  [8]
NAIA_5
 Probe Info 
N.A.  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 84 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info 
19.43  LDD1728  [10]
C026
 Probe Info 
13.00  LDD1732  [10]
C040
 Probe Info 
10.70  LDD1740  [10]
C053
 Probe Info 
6.68  LDD1751  [10]
C063
 Probe Info 
15.45  LDD1760  [10]
C064
 Probe Info 
10.13  LDD1761  [10]
C070
 Probe Info 
20.11  LDD1766  [10]
C082
 Probe Info 
5.74  LDD1774  [10]
C087
 Probe Info 
7.16  LDD1779  [10]
C091
 Probe Info 
10.41  LDD1782  [10]
C094
 Probe Info 
23.10  LDD1785  [10]
C100
 Probe Info 
9.19  LDD1789  [10]
C106
 Probe Info 
21.71  LDD1793  [10]
C107
 Probe Info 
12.30  LDD1794  [10]
C112
 Probe Info 
46.53  LDD1799  [10]
C130
 Probe Info 
6.41  LDD1812  [10]
C135
 Probe Info 
10.70  LDD1817  [10]
C141
 Probe Info 
26.35  LDD1823  [10]
C143
 Probe Info 
21.56  LDD1825  [10]
C147
 Probe Info 
7.57  LDD1829  [10]
C153
 Probe Info 
30.48  LDD1834  [10]
C156
 Probe Info 
6.36  LDD1836  [10]
C159
 Probe Info 
40.50  LDD1839  [10]
C160
 Probe Info 
8.34  LDD1840  [10]
C161
 Probe Info 
20.82  LDD1841  [10]
C163
 Probe Info 
9.13  LDD1843  [10]
C169
 Probe Info 
20.68  LDD1849  [10]
C198
 Probe Info 
17.15  LDD1874  [10]
C201
 Probe Info 
44.94  LDD1877  [10]
C218
 Probe Info 
17.63  LDD1892  [10]
C219
 Probe Info 
6.82  LDD1893  [10]
C220
 Probe Info 
17.15  LDD1894  [10]
C225
 Probe Info 
11.16  LDD1898  [10]
C228
 Probe Info 
22.47  LDD1901  [10]
C231
 Probe Info 
16.34  LDD1904  [10]
C233
 Probe Info 
9.58  LDD1906  [10]
C235
 Probe Info 
20.97  LDD1908  [10]
C240
 Probe Info 
22.01  LDD1913  [10]
C243
 Probe Info 
16.45  LDD1916  [10]
C246
 Probe Info 
30.06  LDD1919  [10]
C249
 Probe Info 
12.73  LDD1922  [10]
C264
 Probe Info 
59.71  LDD1935  [10]
C273
 Probe Info 
6.68  LDD1943  [10]
C277
 Probe Info 
17.88  LDD1947  [10]
C282
 Probe Info 
38.05  LDD1952  [10]
C287
 Probe Info 
40.79  LDD1957  [10]
C288
 Probe Info 
10.27  LDD1958  [10]
C290
 Probe Info 
5.10  LDD1960  [10]
C296
 Probe Info 
47.18  LDD1966  [10]
C305
 Probe Info 
16.80  LDD1974  [10]
C310
 Probe Info 
8.00  LDD1977  [10]
C314
 Probe Info 
22.78  LDD1981  [10]
C349
 Probe Info 
15.35  LDD2010  [10]
C350
 Probe Info 
65.34  LDD2011  [10]
C356
 Probe Info 
15.35  LDD2017  [10]
C357
 Probe Info 
8.22  LDD2018  [10]
C360
 Probe Info 
5.21  LDD2021  [10]
C362
 Probe Info 
99.73  LDD2023  [10]
C363
 Probe Info 
41.07  LDD2024  [10]
C364
 Probe Info 
42.81  LDD2025  [10]
C366
 Probe Info 
6.73  LDD2027  [10]
C367
 Probe Info 
5.17  LDD2028  [10]
C371
 Probe Info 
8.82  LDD2032  [10]
C375
 Probe Info 
7.52  LDD2035  [10]
C376
 Probe Info 
10.56  LDD2036  [10]
C378
 Probe Info 
8.57  LDD2037  [10]
C380
 Probe Info 
14.42  LDD2039  [10]
C382
 Probe Info 
15.89  LDD2041  [10]
C383
 Probe Info 
14.12  LDD2042  [10]
C386
 Probe Info 
9.58  LDD2045  [10]
C388
 Probe Info 
46.85  LDD2047  [10]
C390
 Probe Info 
44.94  LDD2049  [10]
C399
 Probe Info 
33.36  LDD2058  [10]
C403
 Probe Info 
30.91  LDD2061  [10]
C407
 Probe Info 
13.93  LDD2064  [10]
C413
 Probe Info 
33.13  LDD2069  [10]
C419
 Probe Info 
12.91  LDD2074  [10]
C421
 Probe Info 
7.84  LDD2076  [10]
C422
 Probe Info 
9.85  LDD2077  [10]
FFF probe13
 Probe Info 
20.00  LDD0475  [11]
FFF probe14
 Probe Info 
20.00  LDD0477  [11]
DFG-out-4
 Probe Info 
4.90  LDD0075  [12]
Staurosporine capture compound
 Probe Info 
2.00  LDD0083  [13]
OEA-DA
 Probe Info 
20.00  LDD0046  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C93(0.78)  LDD2142  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C93(1.24)  LDD2117  [2]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C93(0.49)  LDD2131  [2]
 LDCM0545  Acetamide MDA-MB-231 C93(0.99)  LDD2138  [2]
 LDCM0520  AKOS000195272 MDA-MB-231 C93(1.08)  LDD2113  [2]
 LDCM0108  Chloroacetamide HeLa H146(0.00); H126(0.00)  LDD0222  [8]
 LDCM0017  DFG-out-2 A431 4.90  LDD0075  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C93(1.51)  LDD1702  [2]
 LDCM0107  IAA HeLa H126(0.00); H146(0.00)  LDD0221  [8]
 LDCM0022  KB02 HCC1806 C93(1.59)  LDD2341  [3]
 LDCM0023  KB03 MDA-MB-231 C93(4.37)  LDD1701  [2]
 LDCM0024  KB05 MV4-11 C93(1.37)  LDD3338  [3]
 LDCM0109  NEM HeLa N.A.  LDD0223  [8]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C93(1.31)  LDD2089  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C93(1.53)  LDD2093  [2]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C93(1.55)  LDD2097  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C93(1.69)  LDD2099  [2]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C93(0.72)  LDD2100  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C93(1.19)  LDD2101  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C93(1.09)  LDD2105  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C93(1.05)  LDD2107  [2]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C93(0.96)  LDD2108  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C93(1.31)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C93(1.14)  LDD2111  [2]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C93(0.57)  LDD2115  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C93(0.50)  LDD2120  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C93(0.90)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C93(1.12)  LDD2127  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C93(0.45)  LDD2128  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C93(1.18)  LDD2129  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C93(0.71)  LDD2133  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C93(0.72)  LDD2134  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C93(1.05)  LDD2135  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C93(1.68)  LDD2136  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C93(3.60)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C93(1.29)  LDD2140  [2]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C93(0.34)  LDD2143  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C93(1.57)  LDD2144  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C93(0.49)  LDD2148  [2]
 LDCM0014  Panhematin K562 3.16  LDD0063  [4]
 LDCM0019  Staurosporine Hep-G2 2.00  LDD0083  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transmembrane emp24 domain-containing protein 10 (TMED10) EMP24/GP25L family P49755
GPCR
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteinase-activated receptor 2 (F2RL1) G-protein coupled receptor 1 family P55085
Extracellular calcium-sensing receptor (CASR) G-protein coupled receptor 3 family P41180
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CD59 glycoprotein (CD59) . P13987

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
7 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
12 Affinity-based probes based on type II kinase inhibitors. J Am Chem Soc. 2012 Nov 21;134(46):19017-25. doi: 10.1021/ja306035v. Epub 2012 Nov 6.
13 Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.
14 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570