Probe Name	Staurosporine capture compound					Probe Info
Probe concentration	50 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	LFQ
Competitor Name	Staurosporine					Competitor Info
Competitor Concentration	1 mM
In Vivo Experiment Model	Model Type			Cell lysate
	Derived Tissue			Liver
	Disease Model		Hepatoblastoma [ICD-11:DB91]
	Model Name		Human hepatoblastoma cell lysate (Hep-G2)

Target ID	Target Name					Binding Ratio
LDTP02638	Proto-oncogene tyrosine-protein kinase Src (SRC)					23.5
LDTP02824	Ribosyldihydronicotinamide dehydrogenase [quinone] (NQO2)					23.0
LDTP11437	Peroxisomal trans-2-enoyl-CoA reductase (PECR)					17.0
LDTP02798	NAD(P)H dehydrogenase [quinone] 1 (NQO1)					12.0
LDTP00491	Aurora kinase A (AURKA)					10.0
LDTP14240	Serine/threonine-protein kinase 24 (STK24)					4.0
LDTP10318	Ubiquitin thioesterase OTUB1 (OTUB1)					4.0
LDTP04051	Nicotinamide phosphoribosyltransferase (NAMPT)					3.5
LDTP01033	UDP-glucose 6-dehydrogenase (UGDH)					3.3
LDTP09396	Minor histocompatibility antigen H13 (HM13)					3.0
LDTP04306	Adenosine 5'-monophosphoramidase HINT1 (HINT1)					2.5
LDTP03725	Replication factor C subunit 4 (RFC4)					2.5
LDTP02677	Protein disulfide-isomerase A4 (PDIA4)					2.4
LDTP06307	Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1)					2.4
LDTP02942	ADP-ribosylation factor 4 (ARF4)					2.3
LDTP04561	ATP-citrate synthase (ACLY)					2.2
LDTP05006	Ras-related protein Rab-1A (RAB1A)					2.1
LDTP00714	26S proteasome non-ATPase regulatory subunit 3 (PSMD3)					2.0
LDTP10514	Cytosolic non-specific dipeptidase (CNDP2)					2.0
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					2.0
LDTP02008	Fructose-bisphosphate aldolase A (ALDOA)					2.0
LDTP05665	Kinesin heavy chain isoform 5A (KIF5A)					2.0
LDTP06477	Methylsterol monooxygenase 1 (MSMO1)					2.0
LDTP02963	Phosphoglycerate mutase 1 (PGAM1)					2.0
LDTP09961	Probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X)					2.0
LDTP09810	Protein NDRG1 (NDRG1)					2.0
LDTP06455	Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL)					2.0
LDTP06612	2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial (DECR1)					.
LDTP00670	3-phosphoinositide-dependent protein kinase 1 (PDPK1)					.
LDTP09428	ATP-dependent RNA helicase DDX54 (DDX54)					.
LDTP10346	Aurora kinase B (AURKB)					.
LDTP01328	CAAX prenyl protease 1 homolog (ZMPSTE24)					.
LDTP03299	Cyclin-dependent kinase 2 (CDK2)					.
LDTP05245	Cyclin-dependent kinase 5 (CDK5)					.
LDTP00697	Cytochrome b5 type B (CYB5B)					.
LDTP03682	DNA replication licensing factor MCM7 (MCM7)					.
LDTP00246	Eukaryotic translation initiation factor 3 subunit F (EIF3F)					.
LDTP05453	Focal adhesion kinase 1 (PTK2)					.
LDTP02569	Glucose-6-phosphate 1-dehydrogenase (G6PD)					.
LDTP12790	Glutaminyl-peptide cyclotransferase-like protein (QPCTL)					.
LDTP05128	Glutathione S-transferase omega-1 (GSTO1)					.
LDTP00089	Glycerol-3-phosphate phosphatase (PGP)					.
LDTP04319	Glycogen synthase kinase-3 beta (GSK3B)					.
LDTP04341	Histamine N-methyltransferase (HNMT)					.
LDTP03388	MAP/microtubule affinity-regulating kinase 3 (MARK3)					.
LDTP08863	Misshapen-like kinase 1 (MINK1)					.
LDTP12610	Obg-like ATPase 1 (OLA1)					.
LDTP05773	Peroxiredoxin-4 (PRDX4)					.
LDTP02375	Poly [ADP-ribose] polymerase 1 (PARP1)					.
LDTP06338	Prostaglandin E synthase 3 (PTGES3)					.
LDTP06227	Prostaglandin reductase 1 (PTGR1)					.
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					.
LDTP00343	Pyridoxal kinase (PDXK)					.
LDTP12878	Regulator of telomere elongation helicase 1 (RTEL1)					.
LDTP01127	Rho-associated protein kinase 2 (ROCK2)					.
LDTP04882	Ribose-phosphate pyrophosphokinase 1 (PRPS1)					.
LDTP13031	Serine/threonine-protein kinase 26 (STK26)					.
LDTP05779	Serine/threonine-protein kinase 3 (STK3)					.
LDTP10367	Serine/threonine-protein kinase greatwall (MASTL)					.
LDTP12968	Serine/threonine-protein kinase MARK1 (MARK1)					.
LDTP07930	Serine/threonine-protein kinase MARK2 (MARK2)					.
LDTP06535	Serine/threonine-protein kinase N1 (PKN1)					.
LDTP06536	Serine/threonine-protein kinase N2 (PKN2)					.
LDTP01751	Serine/threonine-protein kinase PAK 4 (PAK4)					.
LDTP11299	Serine/threonine-protein kinase RIO2 (RIOK2)					.
LDTP13270	Serine/threonine-protein kinase TBK1 (TBK1)					.
LDTP07577	Serine/threonine-protein kinase ULK3 (ULK3)					.
LDTP05991	Spliceosome RNA helicase DDX39B (DDX39B)					.
LDTP07929	Staphylococcal nuclease domain-containing protein 1 (SND1)					.
LDTP13203	STE20/SPS1-related proline-alanine-rich protein kinase (STK39)					.
LDTP03944	Tyrosine-protein kinase CSK (CSK)					.
LDTP02859	Tyrosine-protein kinase Fer (FER)					.
LDTP02231	Tyrosine-protein kinase Yes (YES1)					.
LDTP11675	Ubiquitin-like modifier-activating enzyme 5 (UBA5)					.
LDTP14061	Unconventional myosin-Va (MYO5A)					.
LDTP13609	Unconventional myosin-VI (MYO6)					.
LDTP04096	Vesicle-fusing ATPase (NSF)					.

Target ID	Target Name					Binding Ratio
LDTP00821	Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44)					5.7
LDTP04237	Protein ERGIC-53 (LMAN1)					4.0
LDTP00501	Exportin-1 (XPO1)					2.7
LDTP04393	Annexin A11 (ANXA11)					2.0
LDTP04426	B-cell receptor-associated protein 31 (BCAP31)					2.0
LDTP10989	Copine-1 (CPNE1)					2.0
LDTP03107	Filamin-A (FLNA)					2.0
LDTP01197	Filamin-B (FLNB)					2.0
LDTP06365	Transmembrane emp24 domain-containing protein 2 (TMED2)					2.0
LDTP01936	Apolipoprotein E (APOE)					.
LDTP02972	ATP synthase-coupling factor 6, mitochondrial (ATP5PF)					.
LDTP09149	ATP-binding cassette sub-family F member 1 (ABCF1)					.
LDTP04938	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1)					.
LDTP00014	Extended synaptotagmin-2 (ESYT2)					.
LDTP02735	G2/mitotic-specific cyclin-B1 (CCNB1)					.
LDTP14066	Hypoxia up-regulated protein 1 (HYOU1)					.
LDTP09515	Importin-4 (IPO4)					.
LDTP05305	Large neutral amino acids transporter small subunit 1 (SLC7A5)					.
LDTP14235	Mitochondrial carrier homolog 2 (MTCH2)					.
LDTP08769	Nuclear pore complex protein Nup93 (NUP93)					.
LDTP12559	Potassium voltage-gated channel subfamily H member 7 (KCNH7)					.
LDTP04707	Protein SEC13 homolog (SEC13)					.
LDTP08807	Solute carrier family 66 member 2 (SLC66A2)					.
LDTP05039	Vesicle-associated membrane protein 2 (VAMP2)					.

Target ID	Target Name					Binding Ratio
LDTP08584	Homeobox and leucine zipper protein Homez (HOMEZ)					.
LDTP03903	Signal transducer and activator of transcription 3 (STAT3)					.

Target ID	Target Name					Binding Ratio
LDTP08193	Myopalladin (MYPN)					.

Target ID	Target Name					Binding Ratio
LDTP14216	Coatomer subunit gamma-1 (COPG1)					5.0
LDTP05484	Proteasome activator complex subunit 1 (PSME1)					3.2
LDTP03520	Phosphatidylethanolamine-binding protein 1 (PEBP1)					3.0
LDTP05280	Serine/arginine-rich splicing factor 2 (SRSF2)					3.0
LDTP04294	RNA-binding protein 25 (RBM25)					2.3
LDTP05003	Visinin-like protein 1 (VSNL1)					2.2
LDTP18462	COX assembly mitochondrial protein 2 homolog (CMC2)					2.0
LDTP05257	Receptor expression-enhancing protein 5 (REEP5)					2.0
LDTP13227	Testin (TES)					2.0
LDTP04903	Actin-related protein 2 (ACTR2)					.
LDTP04902	Actin-related protein 3 (ACTR3)					.
LDTP10597	Cilia- and flagella-associated protein 44 (CFAP44)					.
LDTP06542	Cysteine and glycine-rich protein 2 (CSRP2)					.
LDTP03283	Elongation factor 1-beta (EEF1B2)					.
LDTP03499	Elongation factor 1-delta (EEF1D)					.
LDTP04356	Emerin (EMD)					.
LDTP04718	Eukaryotic translation initiation factor 3 subunit B (EIF3B)					.
LDTP07605	La-related protein 1 (LARP1)					.
LDTP11018	Plakophilin-2 (PKP2)					.
LDTP09287	Retinitis pigmentosa 9 protein (RP9)					.
LDTP05802	Serine/arginine-rich splicing factor 6 (SRSF6)					.
LDTP03479	SHC-transforming protein 1 (SHC1)					.
LDTP11331	Sideroflexin-3 (SFXN3)					.
LDTP05677	Splicing factor 3A subunit 3 (SF3A3)					.
LDTP01251	Splicing factor 3B subunit 1 (SF3B1)					.
LDTP12606	Structural maintenance of chromosomes protein 4 (SMC4)					.
LDTP09901	TRAF family member-associated NF-kappa-B activator (TANK)					.
LDTP04703	Tumor protein D52 (TPD52)					.

1	Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.