Details of the Probe

General Information of Probe

Probe ID
LDPC0073
Probe Name
Staurosporine capture compound
Probe Type
PAL-AfBPP Probe
Structure
2D MOL
Ro5 Violations (Lipinski): 5 Molecular Weight (mw) 1383.665
Lipid-water partition coefficient (xlogp) 5.9892
Rotatable Bond Count (rotbonds) 36
Hydrogen Bond Donor Count (hbonddonor) 8
Hydrogen Bond Acceptor Count (hbondacc) 17
Chemical Identifiers
Formula
C70H92N15O13S+
Canonical SMILES
[H]C12CC(N(C)C(=O)CNC(=O)C(CCCCNC(=O)CC(NC(=O)c3ccc(N=[N+]=[N-])cc3)C(=O)NCCCOCCOCCOCCCNC(=O)CCCCC3SCC4([H])NC(=O)NC34[H])[N+](C)(C)C)C(OC)C(C)(O1)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
InChI
InChI=1S/C70H91N15O13S/c1-70-64(94-6)51(38-57(98-70)83-49-19-9-7-17-44(49)59-60-46(39-75-68(60)92)58-45-18-8-10-20-50(45)84(70)63(58)62(59)83)82(2)56(88)40-76-67(91)52(85(3,4)5)21-13-14-28-73-55(87)37-47(77-65(89)42-24-26-43(27-25-42)80-81-71)66(90)74-30-16-32-96-34-36-97-35-33-95-31-15-29-72-54(86)23-12-11-22-53-61-48(41-99-53)78-69(93)79-61/h7-10,17-20,24-27,47-48,51-53,57,61,64H,11-16,21-23,28-41H2,1-6H3,(H7-,72,73,74,75,76,77,78,79,86,87,89,90,91,92,93)/p+1/t47-,48-,51+,52?,53-,57+,61-,64+,70-/m0/s1
InChIKey
UZDQJGYUEMGEFB-OHPGTUDKSA-O

The Probe Interaction Atlas

Target(s) List of this Probe

77 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial (DECR1) LDTP06612 N.A.  LDD0083 
26S proteasome non-ATPase regulatory subunit 3 (PSMD3) LDTP00714 2.00  LDD0083 
3-phosphoinositide-dependent protein kinase 1 (PDPK1) LDTP00670 N.A.  LDD0083 
Adenosine 5'-monophosphoramidase HINT1 (HINT1) LDTP04306 2.50  LDD0083 
ADP-ribosylation factor 4 (ARF4) LDTP02942 2.25  LDD0083 
ATP-citrate synthase (ACLY) LDTP04561 2.17  LDD0083 
ATP-dependent RNA helicase DDX54 (DDX54) LDTP09428 N.A.  LDD0083 
Aurora kinase A (AURKA) LDTP00491 10.00  LDD0083 
Aurora kinase B (AURKB) LDTP10346 N.A.  LDD0083 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 N.A.  LDD0083 
Cyclin-dependent kinase 2 (CDK2) LDTP03299 N.A.  LDD0083 
Cyclin-dependent kinase 5 (CDK5) LDTP05245 N.A.  LDD0083 
Cytochrome b5 type B (CYB5B) LDTP00697 N.A.  LDD0083 
Cytosolic non-specific dipeptidase (CNDP2) LDTP10514 2.00  LDD0083 
Delta(14)-sterol reductase LBR (LBR) LDTP06200 2.00  LDD0083 
DNA replication licensing factor MCM7 (MCM7) LDTP03682 N.A.  LDD0083 
Eukaryotic translation initiation factor 3 subunit F (EIF3F) LDTP00246 N.A.  LDD0083 
Focal adhesion kinase 1 (PTK2) LDTP05453 N.A.  LDD0083 
Fructose-bisphosphate aldolase A (ALDOA) LDTP02008 2.00  LDD0083 
Glucose-6-phosphate 1-dehydrogenase (G6PD) LDTP02569 N.A.  LDD0083 
Glutaminyl-peptide cyclotransferase-like protein (QPCTL) LDTP12790 N.A.  LDD0083 
Glutathione S-transferase omega-1 (GSTO1) LDTP05128 N.A.  LDD0083 
Glycerol-3-phosphate phosphatase (PGP) LDTP00089 N.A.  LDD0083 
Glycogen synthase kinase-3 beta (GSK3B) LDTP04319 N.A.  LDD0083 
Histamine N-methyltransferase (HNMT) LDTP04341 N.A.  LDD0083 
Kinesin heavy chain isoform 5A (KIF5A) LDTP05665 2.00  LDD0083 
MAP/microtubule affinity-regulating kinase 3 (MARK3) LDTP03388 N.A.  LDD0083 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 2.00  LDD0083 
Minor histocompatibility antigen H13 (HM13) LDTP09396 3.00  LDD0083 
Misshapen-like kinase 1 (MINK1) LDTP08863 N.A.  LDD0083 
NAD(P)H dehydrogenase [quinone] 1 (NQO1) LDTP02798 12.00  LDD0083 
Nicotinamide phosphoribosyltransferase (NAMPT) LDTP04051 3.50  LDD0083 
Obg-like ATPase 1 (OLA1) LDTP12610 N.A.  LDD0083 
Peroxiredoxin-4 (PRDX4) LDTP05773 N.A.  LDD0083 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 2.40  LDD0083 
Peroxisomal trans-2-enoyl-CoA reductase (PECR) LDTP11437 17.00  LDD0083 
Phosphoglycerate mutase 1 (PGAM1) LDTP02963 2.00  LDD0083 
Poly [ADP-ribose] polymerase 1 (PARP1) LDTP02375 N.A.  LDD0083 
Probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X) LDTP09961 2.00  LDD0083 
Prostaglandin E synthase 3 (PTGES3) LDTP06338 N.A.  LDD0083 
Prostaglandin reductase 1 (PTGR1) LDTP06227 N.A.  LDD0083 
Protein disulfide-isomerase A4 (PDIA4) LDTP02677 2.44  LDD0083 
Protein NDRG1 (NDRG1) LDTP09810 2.00  LDD0083 
Proto-oncogene tyrosine-protein kinase Src (SRC) LDTP02638 23.50  LDD0083 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 N.A.  LDD0083 
Pyridoxal kinase (PDXK) LDTP00343 N.A.  LDD0083 
Ras-related protein Rab-1A (RAB1A) LDTP05006 2.14  LDD0083 
Regulator of telomere elongation helicase 1 (RTEL1) LDTP12878 N.A.  LDD0083 
Replication factor C subunit 4 (RFC4) LDTP03725 2.50  LDD0083 
Rho-associated protein kinase 2 (ROCK2) LDTP01127 N.A.  LDD0083 
Ribose-phosphate pyrophosphokinase 1 (PRPS1) LDTP04882 N.A.  LDD0083 
Ribosyldihydronicotinamide dehydrogenase [quinone] (NQO2) LDTP02824 23.00  LDD0083 
Serine/threonine-protein kinase 24 (STK24) LDTP14240 4.00  LDD0083 
Serine/threonine-protein kinase 26 (STK26) LDTP13031 N.A.  LDD0083 
Serine/threonine-protein kinase 3 (STK3) LDTP05779 N.A.  LDD0083 
Serine/threonine-protein kinase greatwall (MASTL) LDTP10367 N.A.  LDD0083 
Serine/threonine-protein kinase MARK1 (MARK1) LDTP12968 N.A.  LDD0083 
Serine/threonine-protein kinase MARK2 (MARK2) LDTP07930 N.A.  LDD0083 
Serine/threonine-protein kinase N1 (PKN1) LDTP06535 N.A.  LDD0083 
Serine/threonine-protein kinase N2 (PKN2) LDTP06536 N.A.  LDD0083 
Serine/threonine-protein kinase PAK 4 (PAK4) LDTP01751 N.A.  LDD0083 
Serine/threonine-protein kinase RIO2 (RIOK2) LDTP11299 N.A.  LDD0083 
Serine/threonine-protein kinase TBK1 (TBK1) LDTP13270 N.A.  LDD0083 
Serine/threonine-protein kinase ULK3 (ULK3) LDTP07577 N.A.  LDD0083 
Spliceosome RNA helicase DDX39B (DDX39B) LDTP05991 N.A.  LDD0083 
Staphylococcal nuclease domain-containing protein 1 (SND1) LDTP07929 N.A.  LDD0083 
STE20/SPS1-related proline-alanine-rich protein kinase (STK39) LDTP13203 N.A.  LDD0083 
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL) LDTP06455 2.00  LDD0083 
Tyrosine-protein kinase CSK (CSK) LDTP03944 N.A.  LDD0083 
Tyrosine-protein kinase Fer (FER) LDTP02859 N.A.  LDD0083 
Tyrosine-protein kinase Yes (YES1) LDTP02231 N.A.  LDD0083 
Ubiquitin thioesterase OTUB1 (OTUB1) LDTP10318 4.00  LDD0083 
Ubiquitin-like modifier-activating enzyme 5 (UBA5) LDTP11675 N.A.  LDD0083 
UDP-glucose 6-dehydrogenase (UGDH) LDTP01033 3.33  LDD0083 
Unconventional myosin-Va (MYO5A) LDTP14061 N.A.  LDD0083 
Unconventional myosin-VI (MYO6) LDTP13609 N.A.  LDD0083 
Vesicle-fusing ATPase (NSF) LDTP04096 N.A.  LDD0083 
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⏷ Show the Full List of 77 Enzyme
24 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Annexin A11 (ANXA11) LDTP04393 2.00  LDD0083 
Apolipoprotein E (APOE) LDTP01936 N.A.  LDD0083 
ATP synthase-coupling factor 6, mitochondrial (ATP5PF) LDTP02972 N.A.  LDD0083 
ATP-binding cassette sub-family F member 1 (ABCF1) LDTP09149 N.A.  LDD0083 
B-cell receptor-associated protein 31 (BCAP31) LDTP04426 2.00  LDD0083 
Copine-1 (CPNE1) LDTP10989 2.00  LDD0083 
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) LDTP04938 N.A.  LDD0083 
Exportin-1 (XPO1) LDTP00501 2.67  LDD0083 
Extended synaptotagmin-2 (ESYT2) LDTP00014 N.A.  LDD0083 
Filamin-A (FLNA) LDTP03107 2.00  LDD0083 
Filamin-B (FLNB) LDTP01197 2.00  LDD0083 
G2/mitotic-specific cyclin-B1 (CCNB1) LDTP02735 N.A.  LDD0083 
Hypoxia up-regulated protein 1 (HYOU1) LDTP14066 N.A.  LDD0083 
Importin-4 (IPO4) LDTP09515 N.A.  LDD0083 
Large neutral amino acids transporter small subunit 1 (SLC7A5) LDTP05305 N.A.  LDD0083 
Mitochondrial carrier homolog 2 (MTCH2) LDTP14235 N.A.  LDD0083 
Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) LDTP00821 5.67  LDD0083 
Nuclear pore complex protein Nup93 (NUP93) LDTP08769 N.A.  LDD0083 
Potassium voltage-gated channel subfamily H member 7 (KCNH7) LDTP12559 N.A.  LDD0083 
Protein ERGIC-53 (LMAN1) LDTP04237 4.00  LDD0083 
Protein SEC13 homolog (SEC13) LDTP04707 N.A.  LDD0083 
Solute carrier family 66 member 2 (SLC66A2) LDTP08807 N.A.  LDD0083 
Transmembrane emp24 domain-containing protein 2 (TMED2) LDTP06365 2.00  LDD0083 
Vesicle-associated membrane protein 2 (VAMP2) LDTP05039 N.A.  LDD0083 
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⏷ Show the Full List of 24 Transporter and channel
2 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Homeobox and leucine zipper protein Homez (HOMEZ) LDTP08584 N.A.  LDD0083 
Signal transducer and activator of transcription 3 (STAT3) LDTP03903 N.A.  LDD0083 
------------------------------------------------------------------------------------
1 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Myopalladin (MYPN) LDTP08193 N.A.  LDD0083 
------------------------------------------------------------------------------------
28 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Actin-related protein 2 (ACTR2) LDTP04903 N.A.  LDD0083 
Actin-related protein 3 (ACTR3) LDTP04902 N.A.  LDD0083 
Cilia- and flagella-associated protein 44 (CFAP44) LDTP10597 N.A.  LDD0083 
Coatomer subunit gamma-1 (COPG1) LDTP14216 5.00  LDD0083 
COX assembly mitochondrial protein 2 homolog (CMC2) LDTP18462 2.00  LDD0083 
Cysteine and glycine-rich protein 2 (CSRP2) LDTP06542 N.A.  LDD0083 
Elongation factor 1-beta (EEF1B2) LDTP03283 N.A.  LDD0083 
Elongation factor 1-delta (EEF1D) LDTP03499 N.A.  LDD0083 
Emerin (EMD) LDTP04356 N.A.  LDD0083 
Eukaryotic translation initiation factor 3 subunit B (EIF3B) LDTP04718 N.A.  LDD0083 
La-related protein 1 (LARP1) LDTP07605 N.A.  LDD0083 
Phosphatidylethanolamine-binding protein 1 (PEBP1) LDTP03520 3.00  LDD0083 
Plakophilin-2 (PKP2) LDTP11018 N.A.  LDD0083 
Proteasome activator complex subunit 1 (PSME1) LDTP05484 3.17  LDD0083 
Receptor expression-enhancing protein 5 (REEP5) LDTP05257 2.00  LDD0083 
Retinitis pigmentosa 9 protein (RP9) LDTP09287 N.A.  LDD0083 
RNA-binding protein 25 (RBM25) LDTP04294 2.25  LDD0083 
Serine/arginine-rich splicing factor 2 (SRSF2) LDTP05280 3.00  LDD0083 
Serine/arginine-rich splicing factor 6 (SRSF6) LDTP05802 N.A.  LDD0083 
SHC-transforming protein 1 (SHC1) LDTP03479 N.A.  LDD0083 
Sideroflexin-3 (SFXN3) LDTP11331 N.A.  LDD0083 
Splicing factor 3A subunit 3 (SF3A3) LDTP05677 N.A.  LDD0083 
Splicing factor 3B subunit 1 (SF3B1) LDTP01251 N.A.  LDD0083 
Structural maintenance of chromosomes protein 4 (SMC4) LDTP12606 N.A.  LDD0083 
Testin (TES) LDTP13227 2.00  LDD0083 
TRAF family member-associated NF-kappa-B activator (TANK) LDTP09901 N.A.  LDD0083 
Tumor protein D52 (TPD52) LDTP04703 N.A.  LDD0083 
Visinin-like protein 1 (VSNL1) LDTP05003 2.17  LDD0083 
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⏷ Show the Full List of 28 Other

Competitor(s) Related To This Probe

Competitor ID Name Concentration Cell-system Ref
 LDCM0019  Staurosporine 1 mM Human hepatoblastoma cell lysate (Hep-G2) [1]

Full Information of The Labelling Profiles of This Probe

Quantification: Probe vs (Probe+Competitor)
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
50 uM
Quantitative Method
LFQ
Competitor Name
Staurosporine
 Competitor Info 
Competitor Concentration
1 mM
In Vitro Experiment Model
Model Type
Cell lysate
Derived Tissue
Liver
Disease Model
Hepatoblastoma [ICD-11:DB91]
Model Name
Human hepatoblastoma cell lysate (Hep-G2)
Interaction Atlas ID
 LDD0083 
Download The Altas

References

1 Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.