Details of the Target

General Information of Target

Target ID LDTP11675
Target Name Ubiquitin-like modifier-activating enzyme 5 (UBA5)
Gene Name UBA5
Gene ID 79876
Synonyms
UBE1DC1; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKFTTLLFLAAVAGALVYAEDASSDSTGADPAQEAGTSKPNEEISGPAEPASPPETTTTA
QETSAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGGVPGGKQFIENG
SEFAQKLLKKFSLLKPWA
Target Bioclass
Enzyme
Family
Ubiquitin-activating E1 family, UBA5 subfamily
Subcellular location
Cytoplasm
Function
E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes.
Uniprot ID
Q9GZZ9
Ensemble ID
ENST00000264991.8
HGNC ID
HGNC:23230
ChEMBL ID
CHEMBL2016429

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.N192H .
MCAS SNV: p.E326Q .
MDAMB453 SNV: p.E352K .
NCIH23 SNV: p.E18Ter .
SUPT1 Deletion: p.Q158NfsTer7 DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		2.92  LDD0066  [1]
TH211
 Probe Info  		Y53(8.49)  LDD0257  [2]
STPyne
 Probe Info  		K60(2.60)  LDD2217  [3]
Probe 1
 Probe Info  		Y53(15.39)  LDD3495  [4]
HHS-475
 Probe Info  		Y372(0.72)  LDD0264  [5]
HHS-465
 Probe Info  		Y372(7.14); Y53(10.00)  LDD2237  [6]
DBIA
 Probe Info  		C303(0.91)  LDD0531  [7]
5E-2FA
 Probe Info  		H121(0.00); H133(0.00)  LDD2235  [8]
ATP probe
 Probe Info  		N.A.  LDD0199  [9]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [10]
IA-alkyne
 Probe Info  		C181(0.00); C250(0.00)  LDD0036  [10]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [11]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [11]
Lodoacetamide azide
 Probe Info  		C181(0.00); C250(0.00)  LDD0037  [10]
IPM
 Probe Info  		N.A.  LDD2156  [12]
1c-yne
 Probe Info  		N.A.  LDD0228  [13]
Acrolein
 Probe Info  		N.A.  LDD0217  [14]
m-APA
 Probe Info  		N.A.  LDD0404  [15]
NAIA_5
 Probe Info  		C250(0.00); C181(0.00)  LDD2223  [16]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [17]
Staurosporine capture compound
 Probe Info  		N.A.  LDD0083  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C303(0.91)  LDD0531  [7]
 LDCM0215  AC10 HCT 116 C303(1.26)  LDD0532  [7]
 LDCM0216  AC100 HEK-293T C303(0.91)  LDD0814  [7]
 LDCM0217  AC101 HEK-293T C303(0.93)  LDD0815  [7]
 LDCM0218  AC102 HEK-293T C303(0.99)  LDD0816  [7]
 LDCM0219  AC103 HEK-293T C303(0.86)  LDD0817  [7]
 LDCM0220  AC104 HEK-293T C303(0.72)  LDD0818  [7]
 LDCM0221  AC105 HEK-293T C303(1.21)  LDD0819  [7]
 LDCM0222  AC106 HEK-293T C303(1.12)  LDD0820  [7]
 LDCM0223  AC107 HEK-293T C303(0.80)  LDD0821  [7]
 LDCM0224  AC108 HEK-293T C303(1.20)  LDD0822  [7]
 LDCM0225  AC109 HEK-293T C303(1.09)  LDD0823  [7]
 LDCM0226  AC11 HCT 116 C303(1.15)  LDD0543  [7]
 LDCM0227  AC110 HEK-293T C303(0.57)  LDD0825  [7]
 LDCM0228  AC111 HEK-293T C303(0.56)  LDD0826  [7]
 LDCM0229  AC112 HEK-293T C303(0.56)  LDD0827  [7]
 LDCM0230  AC113 HCT 116 C303(1.03)  LDD0547  [7]
 LDCM0231  AC114 HCT 116 C303(0.90)  LDD0548  [7]
 LDCM0232  AC115 HCT 116 C303(1.01)  LDD0549  [7]
 LDCM0233  AC116 HCT 116 C303(1.03)  LDD0550  [7]
 LDCM0234  AC117 HCT 116 C303(1.06)  LDD0551  [7]
 LDCM0235  AC118 HCT 116 C303(1.10)  LDD0552  [7]
 LDCM0236  AC119 HCT 116 C303(1.15)  LDD0553  [7]
 LDCM0237  AC12 HCT 116 C303(1.26)  LDD0554  [7]
 LDCM0238  AC120 HCT 116 C303(0.82)  LDD0555  [7]
 LDCM0239  AC121 HCT 116 C303(1.16)  LDD0556  [7]
 LDCM0240  AC122 HCT 116 C303(1.02)  LDD0557  [7]
 LDCM0241  AC123 HCT 116 C303(0.86)  LDD0558  [7]
 LDCM0242  AC124 HCT 116 C303(0.99)  LDD0559  [7]
 LDCM0243  AC125 HCT 116 C303(0.94)  LDD0560  [7]
 LDCM0244  AC126 HCT 116 C303(0.99)  LDD0561  [7]
 LDCM0245  AC127 HCT 116 C303(0.93)  LDD0562  [7]
 LDCM0259  AC14 HCT 116 C303(0.93)  LDD0576  [7]
 LDCM0263  AC143 HEK-293T C303(0.60)  LDD0861  [7]
 LDCM0264  AC144 HEK-293T C303(0.50)  LDD0862  [7]
 LDCM0265  AC145 HEK-293T C303(0.51)  LDD0863  [7]
 LDCM0266  AC146 HEK-293T C303(0.50)  LDD0864  [7]
 LDCM0267  AC147 HEK-293T C303(0.47)  LDD0865  [7]
 LDCM0268  AC148 HEK-293T C303(0.48)  LDD0866  [7]
 LDCM0269  AC149 HEK-293T C303(0.86)  LDD0867  [7]
 LDCM0270  AC15 HCT 116 C303(1.12)  LDD0587  [7]
 LDCM0271  AC150 HEK-293T C303(0.71)  LDD0869  [7]
 LDCM0272  AC151 HEK-293T C303(0.63)  LDD0870  [7]
 LDCM0273  AC152 HEK-293T C303(0.61)  LDD0871  [7]
 LDCM0274  AC153 HEK-293T C303(0.67)  LDD0872  [7]
 LDCM0621  AC154 HEK-293T C303(0.73)  LDD2162  [7]
 LDCM0622  AC155 HEK-293T C303(0.63)  LDD2163  [7]
 LDCM0623  AC156 HEK-293T C303(0.39)  LDD2164  [7]
 LDCM0624  AC157 HEK-293T C303(0.42)  LDD2165  [7]
 LDCM0276  AC17 HCT 116 C303(0.99)  LDD0593  [7]
 LDCM0277  AC18 HCT 116 C303(1.20)  LDD0594  [7]
 LDCM0278  AC19 HCT 116 C303(1.28)  LDD0595  [7]
 LDCM0279  AC2 HCT 116 C303(1.11)  LDD0596  [7]
 LDCM0280  AC20 HCT 116 C303(1.08)  LDD0597  [7]
 LDCM0281  AC21 HCT 116 C303(1.00)  LDD0598  [7]
 LDCM0282  AC22 HCT 116 C303(1.15)  LDD0599  [7]
 LDCM0283  AC23 HCT 116 C303(1.20)  LDD0600  [7]
 LDCM0284  AC24 HCT 116 C303(0.98)  LDD0601  [7]
 LDCM0285  AC25 HCT 116 C303(1.30)  LDD0602  [7]
 LDCM0286  AC26 HCT 116 C303(1.47)  LDD0603  [7]
 LDCM0287  AC27 HCT 116 C303(1.05)  LDD0604  [7]
 LDCM0288  AC28 HCT 116 C303(1.38)  LDD0605  [7]
 LDCM0289  AC29 HCT 116 C303(1.18)  LDD0606  [7]
 LDCM0290  AC3 HCT 116 C303(0.89)  LDD0607  [7]
 LDCM0291  AC30 HCT 116 C303(1.09)  LDD0608  [7]
 LDCM0292  AC31 HCT 116 C303(1.11)  LDD0609  [7]
 LDCM0293  AC32 HCT 116 C303(1.41)  LDD0610  [7]
 LDCM0294  AC33 HCT 116 C303(1.13)  LDD0611  [7]
 LDCM0295  AC34 HCT 116 C303(0.92)  LDD0612  [7]
 LDCM0296  AC35 HCT 116 C303(0.55)  LDD0613  [7]
 LDCM0297  AC36 HCT 116 C303(0.67)  LDD0614  [7]
 LDCM0298  AC37 HCT 116 C303(0.72)  LDD0615  [7]
 LDCM0299  AC38 HCT 116 C303(0.38)  LDD0616  [7]
 LDCM0300  AC39 HCT 116 C303(0.44)  LDD0617  [7]
 LDCM0301  AC4 HCT 116 C303(1.03)  LDD0618  [7]
 LDCM0302  AC40 HCT 116 C303(0.60)  LDD0619  [7]
 LDCM0303  AC41 HCT 116 C303(0.36)  LDD0620  [7]
 LDCM0304  AC42 HCT 116 C303(0.53)  LDD0621  [7]
 LDCM0305  AC43 HCT 116 C303(0.34)  LDD0622  [7]
 LDCM0306  AC44 HCT 116 C303(0.39)  LDD0623  [7]
 LDCM0307  AC45 HCT 116 C303(0.27)  LDD0624  [7]
 LDCM0308  AC46 HEK-293T C181(1.03); C250(1.10)  LDD1547  [19]
 LDCM0309  AC47 HEK-293T C181(1.09); C250(0.92)  LDD1548  [19]
 LDCM0310  AC48 HEK-293T C181(0.97)  LDD1549  [19]
 LDCM0311  AC49 HEK-293T C181(0.96); C250(0.95)  LDD1550  [19]
 LDCM0312  AC5 HCT 116 C303(0.84)  LDD0629  [7]
 LDCM0313  AC50 HEK-293T C181(1.24); C250(0.87)  LDD1552  [19]
 LDCM0314  AC51 HEK-293T C181(0.90); C250(0.94)  LDD1553  [19]
 LDCM0315  AC52 HEK-293T C181(0.95); C250(0.94)  LDD1554  [19]
 LDCM0316  AC53 HEK-293T C181(0.88); C250(1.09)  LDD1555  [19]
 LDCM0317  AC54 HEK-293T C181(0.99); C250(1.11)  LDD1556  [19]
 LDCM0318  AC55 HEK-293T C181(1.09); C250(0.99)  LDD1557  [19]
 LDCM0319  AC56 HEK-293T C181(0.98)  LDD1558  [19]
 LDCM0320  AC57 HCT 116 C303(1.80)  LDD0637  [7]
 LDCM0321  AC58 HCT 116 C303(1.34)  LDD0638  [7]
 LDCM0322  AC59 HCT 116 C303(1.22)  LDD0639  [7]
 LDCM0323  AC6 HCT 116 C303(1.21)  LDD0640  [7]
 LDCM0324  AC60 HCT 116 C303(2.72)  LDD0641  [7]
 LDCM0325  AC61 HCT 116 C303(1.47)  LDD0642  [7]
 LDCM0326  AC62 HCT 116 C303(1.82)  LDD0643  [7]
 LDCM0327  AC63 HCT 116 C303(1.81)  LDD0644  [7]
 LDCM0328  AC64 HCT 116 C303(1.91)  LDD0645  [7]
 LDCM0329  AC65 HCT 116 C303(1.23)  LDD0646  [7]
 LDCM0330  AC66 HCT 116 C303(1.74)  LDD0647  [7]
 LDCM0331  AC67 HCT 116 C303(2.04)  LDD0648  [7]
 LDCM0334  AC7 HCT 116 C303(1.90)  LDD0651  [7]
 LDCM0345  AC8 HCT 116 C303(1.46)  LDD0662  [7]
 LDCM0349  AC83 HEK-293T C303(0.98)  LDD0947  [7]
 LDCM0350  AC84 HEK-293T C303(0.76)  LDD0948  [7]
 LDCM0351  AC85 HEK-293T C303(0.83)  LDD0949  [7]
 LDCM0352  AC86 HEK-293T C303(0.83)  LDD0950  [7]
 LDCM0353  AC87 HEK-293T C303(0.76)  LDD0951  [7]
 LDCM0354  AC88 HEK-293T C303(0.75)  LDD0952  [7]
 LDCM0355  AC89 HEK-293T C303(0.69)  LDD0953  [7]
 LDCM0357  AC90 HEK-293T C303(0.78)  LDD0955  [7]
 LDCM0358  AC91 HEK-293T C303(0.81)  LDD0956  [7]
 LDCM0359  AC92 HEK-293T C303(0.81)  LDD0957  [7]
 LDCM0360  AC93 HEK-293T C303(0.54)  LDD0958  [7]
 LDCM0361  AC94 HEK-293T C303(0.53)  LDD0959  [7]
 LDCM0362  AC95 HEK-293T C303(0.81)  LDD0960  [7]
 LDCM0363  AC96 HEK-293T C303(0.56)  LDD0961  [7]
 LDCM0364  AC97 HEK-293T C303(0.36)  LDD0962  [7]
 LDCM0365  AC98 HEK-293T C303(1.05)  LDD0963  [7]
 LDCM0366  AC99 HEK-293T C303(1.10)  LDD0964  [7]
 LDCM0248  AKOS034007472 HCT 116 C303(1.70)  LDD0565  [7]
 LDCM0356  AKOS034007680 HCT 116 C303(1.32)  LDD0673  [7]
 LDCM0275  AKOS034007705 HCT 116 C303(1.01)  LDD0592  [7]
 LDCM0156  Aniline NCI-H1299 N.A.  LDD0404  [15]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [14]
 LDCM0632  CL-Sc Hep-G2 C181(1.29)  LDD2227  [16]
 LDCM0367  CL1 HCT 116 C303(1.22)  LDD0684  [7]
 LDCM0368  CL10 HCT 116 C303(0.96)  LDD0685  [7]
 LDCM0369  CL100 HCT 116 C303(0.99)  LDD0686  [7]
 LDCM0370  CL101 HCT 116 C303(0.80)  LDD0687  [7]
 LDCM0371  CL102 HCT 116 C303(1.07)  LDD0688  [7]
 LDCM0372  CL103 HCT 116 C303(0.77)  LDD0689  [7]
 LDCM0373  CL104 HCT 116 C303(1.24)  LDD0690  [7]
 LDCM0374  CL105 HCT 116 C303(1.03)  LDD0691  [7]
 LDCM0375  CL106 HCT 116 C303(1.13)  LDD0692  [7]
 LDCM0376  CL107 HCT 116 C303(0.83)  LDD0693  [7]
 LDCM0377  CL108 HCT 116 C303(0.98)  LDD0694  [7]
 LDCM0378  CL109 HCT 116 C303(0.92)  LDD0695  [7]
 LDCM0379  CL11 HCT 116 C303(1.24)  LDD0696  [7]
 LDCM0380  CL110 HCT 116 C303(1.00)  LDD0697  [7]
 LDCM0381  CL111 HCT 116 C303(0.97)  LDD0698  [7]
 LDCM0382  CL112 HCT 116 C303(1.37)  LDD0699  [7]
 LDCM0383  CL113 HCT 116 C303(1.18)  LDD0700  [7]
 LDCM0384  CL114 HCT 116 C303(1.19)  LDD0701  [7]
 LDCM0385  CL115 HCT 116 C303(1.08)  LDD0702  [7]
 LDCM0386  CL116 HCT 116 C303(1.10)  LDD0703  [7]
 LDCM0387  CL117 HCT 116 C303(0.23)  LDD0704  [7]
 LDCM0388  CL118 HCT 116 C303(0.44)  LDD0705  [7]
 LDCM0389  CL119 HCT 116 C303(0.80)  LDD0706  [7]
 LDCM0390  CL12 HCT 116 C303(1.81)  LDD0707  [7]
 LDCM0391  CL120 HCT 116 C303(0.45)  LDD0708  [7]
 LDCM0392  CL121 HEK-293T C181(1.12); C250(1.02)  LDD1596  [19]
 LDCM0393  CL122 HEK-293T C181(0.91); C250(0.99)  LDD1597  [19]
 LDCM0394  CL123 HEK-293T C181(1.00); C250(0.96)  LDD1598  [19]
 LDCM0395  CL124 HEK-293T C181(1.04); C250(0.89)  LDD1599  [19]
 LDCM0396  CL125 HCT 116 C303(1.55)  LDD0713  [7]
 LDCM0397  CL126 HCT 116 C303(1.13)  LDD0714  [7]
 LDCM0398  CL127 HCT 116 C303(1.72)  LDD0715  [7]
 LDCM0399  CL128 HCT 116 C303(1.55)  LDD0716  [7]
 LDCM0400  CL13 HCT 116 C303(0.91)  LDD0717  [7]
 LDCM0401  CL14 HCT 116 C303(1.22)  LDD0718  [7]
 LDCM0402  CL15 HCT 116 C303(1.03)  LDD0719  [7]
 LDCM0403  CL16 HCT 116 C303(1.28)  LDD0720  [7]
 LDCM0404  CL17 HEK-293T C181(0.90); C250(0.88)  LDD1608  [19]
 LDCM0405  CL18 HEK-293T C181(1.20); C250(0.99)  LDD1609  [19]
 LDCM0406  CL19 HEK-293T C181(0.84); C250(1.26)  LDD1610  [19]
 LDCM0407  CL2 HCT 116 C303(1.13)  LDD0724  [7]
 LDCM0408  CL20 HEK-293T C181(0.90); C250(1.31)  LDD1612  [19]
 LDCM0409  CL21 HEK-293T C181(0.73); C250(0.93)  LDD1613  [19]
 LDCM0410  CL22 HEK-293T C181(0.72); C250(1.53)  LDD1614  [19]
 LDCM0411  CL23 HEK-293T C181(0.91); C250(1.28)  LDD1615  [19]
 LDCM0412  CL24 HEK-293T C181(0.83)  LDD1616  [19]
 LDCM0413  CL25 HEK-293T C181(1.00); C250(1.21)  LDD1617  [19]
 LDCM0414  CL26 HEK-293T C181(1.05); C250(0.83)  LDD1618  [19]
 LDCM0415  CL27 HEK-293T C181(1.02); C250(0.93)  LDD1619  [19]
 LDCM0416  CL28 HEK-293T C181(0.95); C250(0.94)  LDD1620  [19]
 LDCM0417  CL29 HEK-293T C181(0.92); C250(0.74)  LDD1621  [19]
 LDCM0418  CL3 HCT 116 C303(0.87)  LDD0735  [7]
 LDCM0419  CL30 HEK-293T C181(1.24); C250(0.93)  LDD1623  [19]
 LDCM0420  CL31 HCT 116 C303(1.23)  LDD0737  [7]
 LDCM0421  CL32 HCT 116 C303(1.04)  LDD0738  [7]
 LDCM0422  CL33 HCT 116 C303(1.10)  LDD0739  [7]
 LDCM0423  CL34 HCT 116 C303(1.41)  LDD0740  [7]
 LDCM0424  CL35 HCT 116 C303(1.18)  LDD0741  [7]
 LDCM0425  CL36 HCT 116 C303(1.08)  LDD0742  [7]
 LDCM0426  CL37 HCT 116 C303(1.19)  LDD0743  [7]
 LDCM0428  CL39 HCT 116 C303(0.93)  LDD0745  [7]
 LDCM0429  CL4 HCT 116 C303(1.12)  LDD0746  [7]
 LDCM0430  CL40 HCT 116 C303(1.07)  LDD0747  [7]
 LDCM0431  CL41 HCT 116 C303(1.15)  LDD0748  [7]
 LDCM0432  CL42 HCT 116 C303(0.75)  LDD0749  [7]
 LDCM0433  CL43 HCT 116 C303(1.36)  LDD0750  [7]
 LDCM0434  CL44 HCT 116 C303(1.43)  LDD0751  [7]
 LDCM0435  CL45 HCT 116 C303(1.73)  LDD0752  [7]
 LDCM0436  CL46 HEK-293T C181(1.03); C250(1.83)  LDD1640  [19]
 LDCM0437  CL47 HEK-293T C181(0.92); C250(1.29)  LDD1641  [19]
 LDCM0438  CL48 HEK-293T C181(0.99)  LDD1642  [19]
 LDCM0439  CL49 HEK-293T C181(0.92); C250(1.05)  LDD1643  [19]
 LDCM0440  CL5 HCT 116 C303(1.01)  LDD0757  [7]
 LDCM0441  CL50 HEK-293T C181(1.02); C250(1.14)  LDD1645  [19]
 LDCM0443  CL52 HEK-293T C181(1.03); C250(0.96)  LDD1646  [19]
 LDCM0444  CL53 HEK-293T C181(0.90); C250(1.12)  LDD1647  [19]
 LDCM0445  CL54 HEK-293T C181(1.05); C250(0.92)  LDD1648  [19]
 LDCM0446  CL55 HEK-293T C181(0.94); C250(1.10)  LDD1649  [19]
 LDCM0447  CL56 HEK-293T C181(0.93); C250(1.20)  LDD1650  [19]
 LDCM0448  CL57 HEK-293T C181(0.93); C250(1.02)  LDD1651  [19]
 LDCM0449  CL58 HEK-293T C181(1.18); C250(1.35)  LDD1652  [19]
 LDCM0450  CL59 HEK-293T C181(0.84); C250(1.10)  LDD1653  [19]
 LDCM0451  CL6 HCT 116 C303(0.96)  LDD0768  [7]
 LDCM0452  CL60 HEK-293T C181(1.02)  LDD1655  [19]
 LDCM0453  CL61 HCT 116 C303(1.63)  LDD0770  [7]
 LDCM0454  CL62 HCT 116 C303(2.34)  LDD0771  [7]
 LDCM0455  CL63 HCT 116 C303(0.94)  LDD0772  [7]
 LDCM0456  CL64 HCT 116 C303(1.25)  LDD0773  [7]
 LDCM0457  CL65 HCT 116 C303(1.34)  LDD0774  [7]
 LDCM0458  CL66 HCT 116 C303(1.26)  LDD0775  [7]
 LDCM0459  CL67 HCT 116 C303(1.07)  LDD0776  [7]
 LDCM0460  CL68 HCT 116 C303(0.92)  LDD0777  [7]
 LDCM0461  CL69 HCT 116 C303(1.22)  LDD0778  [7]
 LDCM0462  CL7 HCT 116 C303(0.99)  LDD0779  [7]
 LDCM0463  CL70 HCT 116 C303(1.45)  LDD0780  [7]
 LDCM0464  CL71 HCT 116 C303(1.59)  LDD0781  [7]
 LDCM0465  CL72 HCT 116 C303(1.20)  LDD0782  [7]
 LDCM0466  CL73 HCT 116 C303(1.28)  LDD0783  [7]
 LDCM0467  CL74 HCT 116 C303(1.05)  LDD0784  [7]
 LDCM0469  CL76 HCT 116 C303(0.98)  LDD0786  [7]
 LDCM0470  CL77 HCT 116 C303(0.98)  LDD0787  [7]
 LDCM0471  CL78 HCT 116 C303(0.99)  LDD0788  [7]
 LDCM0472  CL79 HCT 116 C303(1.05)  LDD0789  [7]
 LDCM0473  CL8 HCT 116 C303(0.77)  LDD0790  [7]
 LDCM0474  CL80 HCT 116 C303(0.99)  LDD0791  [7]
 LDCM0475  CL81 HCT 116 C303(1.19)  LDD0792  [7]
 LDCM0476  CL82 HCT 116 C303(0.92)  LDD0793  [7]
 LDCM0477  CL83 HCT 116 C303(0.75)  LDD0794  [7]
 LDCM0478  CL84 HCT 116 C303(0.76)  LDD0795  [7]
 LDCM0479  CL85 HCT 116 C303(0.90)  LDD0796  [7]
 LDCM0480  CL86 HCT 116 C303(0.84)  LDD0797  [7]
 LDCM0481  CL87 HCT 116 C303(0.91)  LDD0798  [7]
 LDCM0482  CL88 HCT 116 C303(0.83)  LDD0799  [7]
 LDCM0483  CL89 HCT 116 C303(0.44)  LDD0800  [7]
 LDCM0484  CL9 HCT 116 C303(1.00)  LDD0801  [7]
 LDCM0485  CL90 HCT 116 C303(0.83)  LDD0802  [7]
 LDCM0486  CL91 HCT 116 C303(1.16)  LDD0803  [7]
 LDCM0487  CL92 HCT 116 C303(1.04)  LDD0804  [7]
 LDCM0488  CL93 HCT 116 C303(1.05)  LDD0805  [7]
 LDCM0489  CL94 HCT 116 C303(0.99)  LDD0806  [7]
 LDCM0490  CL95 HCT 116 C303(0.90)  LDD0807  [7]
 LDCM0491  CL96 HCT 116 C303(1.04)  LDD0808  [7]
 LDCM0492  CL97 HCT 116 C303(1.03)  LDD0809  [7]
 LDCM0493  CL98 HCT 116 C303(1.07)  LDD0810  [7]
 LDCM0494  CL99 HCT 116 C303(1.14)  LDD0811  [7]
 LDCM0495  E2913 HEK-293T C181(1.00); C250(0.92)  LDD1698  [19]
 LDCM0625  F8 Ramos C181(0.63); C250(0.89)  LDD2187  [20]
 LDCM0572  Fragment10 Ramos C181(0.72); C250(0.61)  LDD2189  [20]
 LDCM0573  Fragment11 Ramos C181(2.62); C250(5.70)  LDD2190  [20]
 LDCM0574  Fragment12 Ramos C181(0.70); C250(0.62)  LDD2191  [20]
 LDCM0575  Fragment13 Ramos C181(0.80); C250(0.66)  LDD2192  [20]
 LDCM0576  Fragment14 Ramos C181(1.45); C250(1.03)  LDD2193  [20]
 LDCM0579  Fragment20 Ramos C181(0.85); C250(0.42)  LDD2194  [20]
 LDCM0580  Fragment21 Ramos C181(0.69); C250(0.34)  LDD2195  [20]
 LDCM0582  Fragment23 Ramos C181(0.60); C250(0.43)  LDD2196  [20]
 LDCM0578  Fragment27 Ramos C181(0.87); C250(0.44)  LDD2197  [20]
 LDCM0586  Fragment28 Ramos C181(0.85); C250(0.65)  LDD2198  [20]
 LDCM0588  Fragment30 Ramos C181(1.10); C250(0.25)  LDD2199  [20]
 LDCM0589  Fragment31 Ramos C181(0.99); C250(0.56)  LDD2200  [20]
 LDCM0590  Fragment32 Ramos C181(0.60); C250(1.18)  LDD2201  [20]
 LDCM0468  Fragment33 HCT 116 C303(1.07)  LDD0785  [7]
 LDCM0596  Fragment38 Ramos C181(1.03); C250(0.16)  LDD2203  [20]
 LDCM0566  Fragment4 Ramos C181(0.55); C250(0.37)  LDD2184  [20]
 LDCM0427  Fragment51 HCT 116 C303(0.95)  LDD0744  [7]
 LDCM0610  Fragment52 Ramos C181(0.68); C250(0.21)  LDD2204  [20]
 LDCM0614  Fragment56 Ramos C181(0.81); C250(0.17)  LDD2205  [20]
 LDCM0569  Fragment7 Ramos C181(0.46); C250(0.51)  LDD2186  [20]
 LDCM0571  Fragment9 Ramos C181(0.64); C250(1.10)  LDD2188  [20]
 LDCM0116  HHS-0101 DM93 Y372(0.72)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y372(0.91)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y372(0.73)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y372(0.74)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y372(0.75)  LDD0268  [5]
 LDCM0022  KB02 HEK-293T C181(1.00); C250(0.84)  LDD1492  [19]
 LDCM0023  KB03 HEK-293T C181(1.01); C250(0.93)  LDD1497  [19]
 LDCM0024  KB05 IGR39 C181(4.85)  LDD3315  [21]
 LDCM0131  RA190 MM1.R C181(1.36)  LDD0304  [22]
 LDCM0019  Staurosporine Hep-G2 N.A.  LDD0083  [18]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-aminobutyric acid receptor-associated protein (GABARAP) ATG8 family O95166
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ATG8 family Q9H0R8
Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2) ATG8 family P60520
Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ATG8 family Q9GZQ8
Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C) ATG8 family Q9BXW4
Ubiquitin-fold modifier 1 (UFM1) UFM1 family P61960

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
11 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
12 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
18 Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.
19 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
20 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
21 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
22 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.