Details of the Target

General Information of Target

Target ID LDTP10367
Target Name Serine/threonine-protein kinase greatwall (MASTL)
Gene Name MASTL
Gene ID 84930
Synonyms
GW; GWL; THC2; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; EC 2.7.11.1; Microtubule-associated serine/threonine-protein kinase-like; MAST-L
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGFQPPAALLLRLFLLQGILRLLWGDLAFIPPFIRMSGPAVSASLVGDTEGVTVSLAVLQ
DEAGILPIPTCGVLNNETEDWSVTVIPGAKVLEVTVRWKRGLDWCSSNETDSFSESPCIL
QTLLVSASHNSSCSAHLLIQVEIYANSSLTHNASENVTVIPNQVYQPLGPCPCNLTAGAC
DVRCCCDQECSSNLTTLFRRSCFTGVFGGDVNPPFDQLCSAGTTTRGVPDWFPFLCVQSP
LANTPFLGYFYHGAVSPKQDSSFEVYVDTDAKDFADFGYKQGDPIMTVKKAYFTIPQVSL
AGQCMQNAPVAFLHNFDVKCVTNLELYQERDGIINAKIKNVALGGIVTPKVIYEEATDLD
KFITNTETPLNNGSTPRIVNVEEHYIFKWNNNTISEINVKIFRAEINAHQKGIMTQRFVV
KFLSYNSGNEEELSGNPGYQLGKPVRALNINRMNNVTTLHLWQSAGRGLCTSATFKPILF
GENVLSGCLLEVGINENCTQLRENAVERLDSLIQATHVAMRGNSDYADLSDGWLEIIRVD
APDPGADPLASSVNGMCLDIPAHLSIRILISDAGAVEGITQQEILGVETRFSSVNWQYQC
GLTCEHKADLLPISASVQFIKIPAQLPHPLTRFQINYTEYDCNRNEVCWPQLLYPWTQYY
QGELHSQCVAKGLLLLLFLTLALFLSNPWTRICKAYS
Target Bioclass
Enzyme
Family
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Uniprot ID
Q96GX5
Ensemble ID
ENST00000342386.10
HGNC ID
HGNC:19042
ChEMBL ID
CHEMBL4105826

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.R737Ter .
CAL148 SNV: p.S648F .
CAL33 SNV: p.C348S; p.A349P DBIA    Probe Info 
L428 SNV: p.K440R .
MELJUSO SNV: p.I186V; p.L235F .
NCIH1975 SNV: p.R592G DBIA    Probe Info 
PC9 SNV: p.S597F .
RKO SNV: p.P723Q .
SW403 SNV: p.Q785K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		12.93  LDD0402  [1]
AHL-Pu-1
 Probe Info  		C555(2.12); C238(2.29)  LDD0169  [2]
DBIA
 Probe Info  		C534(1.02); C540(1.02)  LDD0078  [3]
AMP probe
 Probe Info  		N.A.  LDD0200  [4]
ATP probe
 Probe Info  		K62(0.00); K48(0.00); K179(0.00)  LDD0199  [4]
IA-alkyne
 Probe Info  		C455(0.00); C465(0.00)  LDD0165  [5]
Compound 10
 Probe Info  		N.A.  LDD2216  [6]
Compound 11
 Probe Info  		N.A.  LDD2213  [6]
IPM
 Probe Info  		C285(0.00); C455(0.00)  LDD0147  [7]
TFBX
 Probe Info  		C271(0.00); C285(0.00); C385(0.00)  LDD0148  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		8.22  LDD0476  [10]
FFF probe3
 Probe Info  		9.73  LDD0465  [10]
Staurosporine capture compound
 Probe Info  		N.A.  LDD0083  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C540(2.61)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C555(2.12); C238(2.29)  LDD0169  [2]
 LDCM0214  AC1 HCT 116 C503(0.87)  LDD0531  [3]
 LDCM0215  AC10 HCT 116 C503(0.75)  LDD0532  [3]
 LDCM0216  AC100 HCT 116 C503(0.69)  LDD0533  [3]
 LDCM0217  AC101 HCT 116 C503(0.60)  LDD0534  [3]
 LDCM0218  AC102 HCT 116 C503(1.03)  LDD0535  [3]
 LDCM0219  AC103 HCT 116 C503(1.19)  LDD0536  [3]
 LDCM0220  AC104 HCT 116 C503(1.80)  LDD0537  [3]
 LDCM0221  AC105 HCT 116 C503(1.03)  LDD0538  [3]
 LDCM0222  AC106 HCT 116 C503(1.28)  LDD0539  [3]
 LDCM0223  AC107 HCT 116 C503(0.93)  LDD0540  [3]
 LDCM0224  AC108 HCT 116 C503(1.43)  LDD0541  [3]
 LDCM0225  AC109 HCT 116 C503(1.31)  LDD0542  [3]
 LDCM0226  AC11 HCT 116 C503(0.94)  LDD0543  [3]
 LDCM0227  AC110 HCT 116 C503(1.07)  LDD0544  [3]
 LDCM0228  AC111 HCT 116 C503(1.28)  LDD0545  [3]
 LDCM0229  AC112 HCT 116 C503(0.97)  LDD0546  [3]
 LDCM0230  AC113 PaTu 8988t C503(0.92)  LDD1109  [3]
 LDCM0231  AC114 PaTu 8988t C503(0.82)  LDD1110  [3]
 LDCM0232  AC115 PaTu 8988t C503(1.08)  LDD1111  [3]
 LDCM0233  AC116 PaTu 8988t C503(0.85)  LDD1112  [3]
 LDCM0234  AC117 PaTu 8988t C503(0.97)  LDD1113  [3]
 LDCM0235  AC118 PaTu 8988t C503(1.02)  LDD1114  [3]
 LDCM0236  AC119 PaTu 8988t C503(0.98)  LDD1115  [3]
 LDCM0237  AC12 HCT 116 C503(0.81)  LDD0554  [3]
 LDCM0238  AC120 PaTu 8988t C503(0.71)  LDD1117  [3]
 LDCM0239  AC121 PaTu 8988t C503(1.02)  LDD1118  [3]
 LDCM0240  AC122 PaTu 8988t C503(0.80)  LDD1119  [3]
 LDCM0241  AC123 PaTu 8988t C503(0.78)  LDD1120  [3]
 LDCM0242  AC124 PaTu 8988t C503(1.22)  LDD1121  [3]
 LDCM0243  AC125 PaTu 8988t C503(0.95)  LDD1122  [3]
 LDCM0244  AC126 PaTu 8988t C503(1.11)  LDD1123  [3]
 LDCM0245  AC127 PaTu 8988t C503(1.04)  LDD1124  [3]
 LDCM0246  AC128 PaTu 8988t C238(1.03); C251(1.03)  LDD1125  [3]
 LDCM0247  AC129 PaTu 8988t C238(0.91); C251(0.91)  LDD1126  [3]
 LDCM0249  AC130 PaTu 8988t C238(1.01); C251(1.01)  LDD1128  [3]
 LDCM0250  AC131 PaTu 8988t C238(1.02); C251(1.02)  LDD1129  [3]
 LDCM0251  AC132 PaTu 8988t C238(1.17); C251(1.17)  LDD1130  [3]
 LDCM0252  AC133 PaTu 8988t C238(0.96); C251(0.96)  LDD1131  [3]
 LDCM0253  AC134 PaTu 8988t C238(0.86); C251(0.86)  LDD1132  [3]
 LDCM0254  AC135 PaTu 8988t C238(0.94); C251(0.94)  LDD1133  [3]
 LDCM0255  AC136 PaTu 8988t C238(0.84); C251(0.84)  LDD1134  [3]
 LDCM0256  AC137 PaTu 8988t C238(1.14); C251(1.14)  LDD1135  [3]
 LDCM0257  AC138 PaTu 8988t C238(1.00); C251(1.00)  LDD1136  [3]
 LDCM0258  AC139 PaTu 8988t C238(0.85); C251(0.85)  LDD1137  [3]
 LDCM0259  AC14 HCT 116 C503(0.86)  LDD0576  [3]
 LDCM0260  AC140 PaTu 8988t C238(1.03); C251(1.03)  LDD1139  [3]
 LDCM0261  AC141 PaTu 8988t C238(1.03); C251(1.03)  LDD1140  [3]
 LDCM0262  AC142 PaTu 8988t C238(1.04); C251(1.04)  LDD1141  [3]
 LDCM0263  AC143 HCT 116 C503(0.82)  LDD0580  [3]
 LDCM0264  AC144 HCT 116 C503(1.32)  LDD0581  [3]
 LDCM0265  AC145 HCT 116 C503(0.72)  LDD0582  [3]
 LDCM0266  AC146 HCT 116 C503(0.92)  LDD0583  [3]
 LDCM0267  AC147 HCT 116 C503(1.24)  LDD0584  [3]
 LDCM0268  AC148 HCT 116 C503(1.73)  LDD0585  [3]
 LDCM0269  AC149 HCT 116 C503(2.33)  LDD0586  [3]
 LDCM0270  AC15 HCT 116 C503(0.79)  LDD0587  [3]
 LDCM0271  AC150 HCT 116 C503(1.50)  LDD0588  [3]
 LDCM0272  AC151 HCT 116 C503(0.99)  LDD0589  [3]
 LDCM0273  AC152 HCT 116 C503(1.22)  LDD0590  [3]
 LDCM0274  AC153 HCT 116 C503(2.37)  LDD0591  [3]
 LDCM0621  AC154 HCT 116 C503(1.64)  LDD2158  [3]
 LDCM0622  AC155 HCT 116 C503(1.37)  LDD2159  [3]
 LDCM0623  AC156 HCT 116 C503(1.92)  LDD2160  [3]
 LDCM0624  AC157 HCT 116 C503(2.00)  LDD2161  [3]
 LDCM0276  AC17 HCT 116 C503(1.29)  LDD0593  [3]
 LDCM0277  AC18 HCT 116 C503(0.97)  LDD0594  [3]
 LDCM0278  AC19 HCT 116 C503(0.98)  LDD0595  [3]
 LDCM0279  AC2 HCT 116 C503(0.92)  LDD0596  [3]
 LDCM0280  AC20 HCT 116 C503(1.06)  LDD0597  [3]
 LDCM0281  AC21 HCT 116 C503(1.27)  LDD0598  [3]
 LDCM0282  AC22 HCT 116 C503(1.04)  LDD0599  [3]
 LDCM0283  AC23 HCT 116 C503(1.16)  LDD0600  [3]
 LDCM0284  AC24 HCT 116 C503(1.15)  LDD0601  [3]
 LDCM0285  AC25 HCT 116 C503(0.96)  LDD0602  [3]
 LDCM0286  AC26 HCT 116 C503(1.67)  LDD0603  [3]
 LDCM0287  AC27 HCT 116 C503(1.22)  LDD0604  [3]
 LDCM0288  AC28 HCT 116 C503(1.13)  LDD0605  [3]
 LDCM0289  AC29 HCT 116 C503(1.81)  LDD0606  [3]
 LDCM0290  AC3 HCT 116 C503(0.94)  LDD0607  [3]
 LDCM0291  AC30 HCT 116 C503(1.13)  LDD0608  [3]
 LDCM0292  AC31 HCT 116 C503(1.97)  LDD0609  [3]
 LDCM0293  AC32 HCT 116 C503(1.89)  LDD0610  [3]
 LDCM0294  AC33 HCT 116 C503(1.30)  LDD0611  [3]
 LDCM0295  AC34 HCT 116 C503(2.46)  LDD0612  [3]
 LDCM0296  AC35 HCT 116 C503(0.81)  LDD0613  [3]
 LDCM0297  AC36 HCT 116 C503(1.36)  LDD0614  [3]
 LDCM0298  AC37 HCT 116 C503(1.01)  LDD0615  [3]
 LDCM0299  AC38 HCT 116 C503(1.28)  LDD0616  [3]
 LDCM0300  AC39 HCT 116 C503(1.39)  LDD0617  [3]
 LDCM0301  AC4 HCT 116 C503(0.86)  LDD0618  [3]
 LDCM0302  AC40 HCT 116 C503(1.55)  LDD0619  [3]
 LDCM0303  AC41 HCT 116 C503(1.11)  LDD0620  [3]
 LDCM0304  AC42 HCT 116 C503(0.98)  LDD0621  [3]
 LDCM0305  AC43 HCT 116 C503(1.37)  LDD0622  [3]
 LDCM0306  AC44 HCT 116 C503(1.85)  LDD0623  [3]
 LDCM0307  AC45 HCT 116 C503(2.62)  LDD0624  [3]
 LDCM0308  AC46 HCT 116 C503(1.02)  LDD0625  [3]
 LDCM0309  AC47 HCT 116 C503(0.90)  LDD0626  [3]
 LDCM0310  AC48 HCT 116 C503(1.02)  LDD0627  [3]
 LDCM0311  AC49 HCT 116 C503(1.14)  LDD0628  [3]
 LDCM0312  AC5 HCT 116 C503(0.80)  LDD0629  [3]
 LDCM0313  AC50 HCT 116 C503(0.94)  LDD0630  [3]
 LDCM0314  AC51 HCT 116 C503(0.76)  LDD0631  [3]
 LDCM0315  AC52 HCT 116 C503(0.92)  LDD0632  [3]
 LDCM0316  AC53 HCT 116 C503(1.17)  LDD0633  [3]
 LDCM0317  AC54 HCT 116 C503(1.12)  LDD0634  [3]
 LDCM0318  AC55 HCT 116 C503(1.11)  LDD0635  [3]
 LDCM0319  AC56 HCT 116 C503(1.58)  LDD0636  [3]
 LDCM0320  AC57 HEK-293T C455(1.44)  LDD0918  [3]
 LDCM0321  AC58 HEK-293T C455(1.21)  LDD0919  [3]
 LDCM0322  AC59 HEK-293T C455(1.37)  LDD0920  [3]
 LDCM0323  AC6 HCT 116 C503(0.82)  LDD0640  [3]
 LDCM0324  AC60 HEK-293T C455(1.81)  LDD0922  [3]
 LDCM0325  AC61 HEK-293T C455(1.82)  LDD0923  [3]
 LDCM0326  AC62 HEK-293T C455(1.26)  LDD0924  [3]
 LDCM0327  AC63 HEK-293T C455(0.92)  LDD0925  [3]
 LDCM0328  AC64 HEK-293T C455(1.68)  LDD0926  [3]
 LDCM0329  AC65 HEK-293T C455(1.53)  LDD0927  [3]
 LDCM0330  AC66 HEK-293T C455(1.41)  LDD0928  [3]
 LDCM0331  AC67 HEK-293T C455(1.23)  LDD0929  [3]
 LDCM0332  AC68 PaTu 8988t C238(0.89); C251(0.89); C503(1.03)  LDD1211  [3]
 LDCM0333  AC69 PaTu 8988t C238(0.85); C251(0.85); C503(1.10)  LDD1212  [3]
 LDCM0334  AC7 HCT 116 C503(0.87)  LDD0651  [3]
 LDCM0335  AC70 PaTu 8988t C238(0.90); C251(0.90); C503(1.05)  LDD1214  [3]
 LDCM0336  AC71 PaTu 8988t C238(0.83); C251(0.83); C503(0.89)  LDD1215  [3]
 LDCM0337  AC72 PaTu 8988t C238(0.78); C251(0.78); C503(0.94)  LDD1216  [3]
 LDCM0338  AC73 PaTu 8988t C238(0.82); C251(0.82); C503(1.05)  LDD1217  [3]
 LDCM0339  AC74 PaTu 8988t C238(0.86); C251(0.86); C503(1.00)  LDD1218  [3]
 LDCM0340  AC75 PaTu 8988t C238(0.93); C251(0.93); C503(1.21)  LDD1219  [3]
 LDCM0341  AC76 PaTu 8988t C238(0.83); C251(0.83); C503(0.94)  LDD1220  [3]
 LDCM0342  AC77 PaTu 8988t C238(0.87); C251(0.87); C503(0.96)  LDD1221  [3]
 LDCM0343  AC78 PaTu 8988t C238(0.88); C251(0.88); C503(1.01)  LDD1222  [3]
 LDCM0344  AC79 PaTu 8988t C238(0.88); C251(0.88); C503(1.24)  LDD1223  [3]
 LDCM0345  AC8 HCT 116 C503(0.79)  LDD0662  [3]
 LDCM0346  AC80 PaTu 8988t C238(0.84); C251(0.84); C503(0.95)  LDD1225  [3]
 LDCM0347  AC81 PaTu 8988t C238(1.05); C251(1.05); C503(0.98)  LDD1226  [3]
 LDCM0348  AC82 PaTu 8988t C238(0.97); C251(0.97); C503(0.94)  LDD1227  [3]
 LDCM0349  AC83 HCT 116 C503(1.34)  LDD0666  [3]
 LDCM0350  AC84 HCT 116 C503(1.26)  LDD0667  [3]
 LDCM0351  AC85 HCT 116 C503(1.22)  LDD0668  [3]
 LDCM0352  AC86 HCT 116 C503(1.49)  LDD0669  [3]
 LDCM0353  AC87 HCT 116 C503(1.35)  LDD0670  [3]
 LDCM0354  AC88 HCT 116 C503(1.50)  LDD0671  [3]
 LDCM0355  AC89 HCT 116 C503(1.45)  LDD0672  [3]
 LDCM0357  AC90 HCT 116 C503(1.52)  LDD0674  [3]
 LDCM0358  AC91 HCT 116 C503(1.16)  LDD0675  [3]
 LDCM0359  AC92 HCT 116 C503(1.13)  LDD0676  [3]
 LDCM0360  AC93 HCT 116 C503(1.21)  LDD0677  [3]
 LDCM0361  AC94 HCT 116 C503(0.98)  LDD0678  [3]
 LDCM0362  AC95 HCT 116 C503(1.50)  LDD0679  [3]
 LDCM0363  AC96 HCT 116 C503(0.90)  LDD0680  [3]
 LDCM0364  AC97 HCT 116 C503(1.40)  LDD0681  [3]
 LDCM0365  AC98 HCT 116 C503(1.54)  LDD0682  [3]
 LDCM0366  AC99 HCT 116 C503(1.06)  LDD0683  [3]
 LDCM0248  AKOS034007472 HCT 116 C503(0.92)  LDD0565  [3]
 LDCM0356  AKOS034007680 HCT 116 C503(0.91)  LDD0673  [3]
 LDCM0275  AKOS034007705 HCT 116 C503(0.71)  LDD0592  [3]
 LDCM0156  Aniline NCI-H1299 12.78  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C534(1.02); C540(1.02)  LDD0078  [3]
 LDCM0632  CL-Sc Hep-G2 C455(2.28)  LDD2227  [9]
 LDCM0367  CL1 HCT 116 C503(1.03)  LDD0684  [3]
 LDCM0368  CL10 HCT 116 C503(1.13)  LDD0685  [3]
 LDCM0369  CL100 HCT 116 C503(0.94)  LDD0686  [3]
 LDCM0370  CL101 HCT 116 C503(0.79)  LDD0687  [3]
 LDCM0371  CL102 HCT 116 C503(0.77)  LDD0688  [3]
 LDCM0372  CL103 HCT 116 C503(0.80)  LDD0689  [3]
 LDCM0373  CL104 HCT 116 C503(0.80)  LDD0690  [3]
 LDCM0374  CL105 HCT 116 C503(1.19)  LDD0691  [3]
 LDCM0375  CL106 HCT 116 C503(1.63)  LDD0692  [3]
 LDCM0376  CL107 HCT 116 C503(1.19)  LDD0693  [3]
 LDCM0377  CL108 HCT 116 C503(1.80)  LDD0694  [3]
 LDCM0378  CL109 HCT 116 C503(1.42)  LDD0695  [3]
 LDCM0379  CL11 HCT 116 C503(1.34)  LDD0696  [3]
 LDCM0380  CL110 HCT 116 C503(1.35)  LDD0697  [3]
 LDCM0381  CL111 HCT 116 C503(1.62)  LDD0698  [3]
 LDCM0382  CL112 HCT 116 C503(1.11)  LDD0699  [3]
 LDCM0383  CL113 HCT 116 C503(1.23)  LDD0700  [3]
 LDCM0384  CL114 HCT 116 C503(1.12)  LDD0701  [3]
 LDCM0385  CL115 HCT 116 C503(1.28)  LDD0702  [3]
 LDCM0386  CL116 HCT 116 C503(1.29)  LDD0703  [3]
 LDCM0387  CL117 HCT 116 C503(1.72)  LDD0704  [3]
 LDCM0388  CL118 HCT 116 C503(0.83)  LDD0705  [3]
 LDCM0389  CL119 HCT 116 C503(1.03)  LDD0706  [3]
 LDCM0390  CL12 HCT 116 C503(1.13)  LDD0707  [3]
 LDCM0391  CL120 HCT 116 C503(1.69)  LDD0708  [3]
 LDCM0392  CL121 HCT 116 C503(1.17)  LDD0709  [3]
 LDCM0393  CL122 HCT 116 C503(0.99)  LDD0710  [3]
 LDCM0394  CL123 HCT 116 C503(0.92)  LDD0711  [3]
 LDCM0395  CL124 HCT 116 C503(1.17)  LDD0712  [3]
 LDCM0396  CL125 HEK-293T C455(2.78)  LDD0994  [3]
 LDCM0397  CL126 HEK-293T C455(1.70)  LDD0995  [3]
 LDCM0398  CL127 HEK-293T C455(1.46)  LDD0996  [3]
 LDCM0399  CL128 HEK-293T C455(1.09)  LDD0997  [3]
 LDCM0400  CL13 HCT 116 C503(0.80)  LDD0717  [3]
 LDCM0401  CL14 HCT 116 C503(0.90)  LDD0718  [3]
 LDCM0402  CL15 HCT 116 C503(0.84)  LDD0719  [3]
 LDCM0403  CL16 HCT 116 C503(1.11)  LDD0720  [3]
 LDCM0404  CL17 HCT 116 C503(0.88)  LDD0721  [3]
 LDCM0405  CL18 HCT 116 C503(1.03)  LDD0722  [3]
 LDCM0406  CL19 HCT 116 C503(0.89)  LDD0723  [3]
 LDCM0407  CL2 HCT 116 C503(1.12)  LDD0724  [3]
 LDCM0408  CL20 HCT 116 C503(0.90)  LDD0725  [3]
 LDCM0409  CL21 HCT 116 C503(0.85)  LDD0726  [3]
 LDCM0410  CL22 HCT 116 C503(0.84)  LDD0727  [3]
 LDCM0411  CL23 HCT 116 C503(1.13)  LDD0728  [3]
 LDCM0412  CL24 HCT 116 C503(0.91)  LDD0729  [3]
 LDCM0413  CL25 HCT 116 C503(1.03)  LDD0730  [3]
 LDCM0414  CL26 HCT 116 C503(1.06)  LDD0731  [3]
 LDCM0415  CL27 HCT 116 C503(1.01)  LDD0732  [3]
 LDCM0416  CL28 HCT 116 C503(0.97)  LDD0733  [3]
 LDCM0417  CL29 HCT 116 C503(0.98)  LDD0734  [3]
 LDCM0418  CL3 HCT 116 C503(1.06)  LDD0735  [3]
 LDCM0419  CL30 HCT 116 C503(0.92)  LDD0736  [3]
 LDCM0420  CL31 PaTu 8988t C238(0.82); C251(0.82); C503(0.99)  LDD1299  [3]
 LDCM0421  CL32 PaTu 8988t C238(0.61); C251(0.61); C503(1.06)  LDD1300  [3]
 LDCM0422  CL33 PaTu 8988t C238(3.09); C251(3.09); C503(0.98)  LDD1301  [3]
 LDCM0423  CL34 PaTu 8988t C238(0.75); C251(0.75); C503(1.22)  LDD1302  [3]
 LDCM0424  CL35 PaTu 8988t C238(0.73); C251(0.73); C503(1.45)  LDD1303  [3]
 LDCM0425  CL36 PaTu 8988t C238(0.70); C251(0.70); C503(1.74)  LDD1304  [3]
 LDCM0426  CL37 PaTu 8988t C238(0.95); C251(0.95); C503(1.49)  LDD1305  [3]
 LDCM0428  CL39 PaTu 8988t C238(0.69); C251(0.69); C503(1.11)  LDD1307  [3]
 LDCM0429  CL4 HCT 116 C503(0.92)  LDD0746  [3]
 LDCM0430  CL40 PaTu 8988t C238(1.19); C251(1.19); C503(1.15)  LDD1309  [3]
 LDCM0431  CL41 PaTu 8988t C238(1.31); C251(1.31); C503(0.98)  LDD1310  [3]
 LDCM0432  CL42 PaTu 8988t C238(0.96); C251(0.96); C503(1.18)  LDD1311  [3]
 LDCM0433  CL43 PaTu 8988t C238(1.05); C251(1.05); C503(1.17)  LDD1312  [3]
 LDCM0434  CL44 PaTu 8988t C238(1.27); C251(1.27); C503(1.44)  LDD1313  [3]
 LDCM0435  CL45 PaTu 8988t C238(0.95); C251(0.95); C503(1.25)  LDD1314  [3]
 LDCM0436  CL46 HCT 116 C503(1.05)  LDD0753  [3]
 LDCM0437  CL47 HCT 116 C503(1.01)  LDD0754  [3]
 LDCM0438  CL48 HCT 116 C503(1.14)  LDD0755  [3]
 LDCM0439  CL49 HCT 116 C503(1.03)  LDD0756  [3]
 LDCM0440  CL5 HCT 116 C503(0.89)  LDD0757  [3]
 LDCM0441  CL50 HCT 116 C503(1.32)  LDD0758  [3]
 LDCM0442  CL51 HCT 116 C503(1.20)  LDD0759  [3]
 LDCM0443  CL52 HCT 116 C503(1.34)  LDD0760  [3]
 LDCM0444  CL53 HCT 116 C503(0.96)  LDD0761  [3]
 LDCM0445  CL54 HCT 116 C503(0.93)  LDD0762  [3]
 LDCM0446  CL55 HCT 116 C503(1.01)  LDD0763  [3]
 LDCM0447  CL56 HCT 116 C503(0.85)  LDD0764  [3]
 LDCM0448  CL57 HCT 116 C503(1.04)  LDD0765  [3]
 LDCM0449  CL58 HCT 116 C503(0.93)  LDD0766  [3]
 LDCM0450  CL59 HCT 116 C503(0.96)  LDD0767  [3]
 LDCM0451  CL6 HCT 116 C503(0.99)  LDD0768  [3]
 LDCM0452  CL60 HCT 116 C503(1.04)  LDD0769  [3]
 LDCM0453  CL61 HCT 116 C503(1.53)  LDD0770  [3]
 LDCM0454  CL62 HCT 116 C503(1.19)  LDD0771  [3]
 LDCM0455  CL63 HCT 116 C503(1.27)  LDD0772  [3]
 LDCM0456  CL64 HCT 116 C503(1.89)  LDD0773  [3]
 LDCM0457  CL65 HCT 116 C503(1.54)  LDD0774  [3]
 LDCM0458  CL66 HCT 116 C503(1.87)  LDD0775  [3]
 LDCM0459  CL67 HCT 116 C503(1.86)  LDD0776  [3]
 LDCM0460  CL68 HCT 116 C503(1.63)  LDD0777  [3]
 LDCM0461  CL69 HCT 116 C503(1.63)  LDD0778  [3]
 LDCM0462  CL7 HCT 116 C503(0.95)  LDD0779  [3]
 LDCM0463  CL70 HCT 116 C503(1.23)  LDD0780  [3]
 LDCM0464  CL71 HCT 116 C503(1.34)  LDD0781  [3]
 LDCM0465  CL72 HCT 116 C503(1.39)  LDD0782  [3]
 LDCM0466  CL73 HCT 116 C503(1.22)  LDD0783  [3]
 LDCM0467  CL74 HCT 116 C503(2.00)  LDD0784  [3]
 LDCM0469  CL76 HCT 116 C503(0.90)  LDD0786  [3]
 LDCM0470  CL77 HCT 116 C503(0.75)  LDD0787  [3]
 LDCM0471  CL78 HCT 116 C503(0.90)  LDD0788  [3]
 LDCM0472  CL79 HCT 116 C503(0.87)  LDD0789  [3]
 LDCM0473  CL8 HCT 116 C503(0.77)  LDD0790  [3]
 LDCM0474  CL80 HCT 116 C503(0.87)  LDD0791  [3]
 LDCM0475  CL81 HCT 116 C503(0.80)  LDD0792  [3]
 LDCM0476  CL82 HCT 116 C503(0.77)  LDD0793  [3]
 LDCM0477  CL83 HCT 116 C503(0.86)  LDD0794  [3]
 LDCM0478  CL84 HCT 116 C503(0.86)  LDD0795  [3]
 LDCM0479  CL85 HCT 116 C503(0.89)  LDD0796  [3]
 LDCM0480  CL86 HCT 116 C503(0.92)  LDD0797  [3]
 LDCM0481  CL87 HCT 116 C503(0.88)  LDD0798  [3]
 LDCM0482  CL88 HCT 116 C503(0.95)  LDD0799  [3]
 LDCM0483  CL89 HCT 116 C503(0.86)  LDD0800  [3]
 LDCM0484  CL9 HCT 116 C503(1.06)  LDD0801  [3]
 LDCM0485  CL90 HCT 116 C503(0.91)  LDD0802  [3]
 LDCM0486  CL91 HCT 116 C503(0.91)  LDD0803  [3]
 LDCM0487  CL92 HCT 116 C503(0.83)  LDD0804  [3]
 LDCM0488  CL93 HCT 116 C503(0.78)  LDD0805  [3]
 LDCM0489  CL94 HCT 116 C503(0.94)  LDD0806  [3]
 LDCM0490  CL95 HCT 116 C503(0.86)  LDD0807  [3]
 LDCM0491  CL96 HCT 116 C503(0.81)  LDD0808  [3]
 LDCM0492  CL97 HCT 116 C503(0.80)  LDD0809  [3]
 LDCM0493  CL98 HCT 116 C503(0.95)  LDD0810  [3]
 LDCM0494  CL99 HCT 116 C503(0.91)  LDD0811  [3]
 LDCM0634  CY-0357 Hep-G2 C555(0.76); C455(0.38)  LDD2228  [9]
 LDCM0495  E2913 HEK-293T C503(0.96); C385(1.04)  LDD1698  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C285(0.14)  LDD1702  [13]
 LDCM0468  Fragment33 HCT 116 C503(1.35)  LDD0785  [3]
 LDCM0427  Fragment51 PaTu 8988t C238(1.83); C251(1.83); C503(1.34)  LDD1306  [3]
 LDCM0022  KB02 HCT 116 C455(2.24); C534(3.01); C540(3.01); C503(2.09)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C455(1.50); C534(2.11); C540(2.11); C503(1.07)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C455(2.49); C534(2.30); C540(2.30); C503(2.29)  LDD0082  [3]
 LDCM0019  Staurosporine Hep-G2 N.A.  LDD0083  [11]
 LDCM0021  THZ1 HCT 116 C534(1.04); C540(1.04)  LDD2173  [3]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761